RU2515048C1 - Method of culturing bifidobacteria in milk - Google Patents

Abstract

FIELD: chemistry.

SUBSTANCE: invention relates to biotechnology and can be used in the dairy industry. Pasteurised milk is cooled to a given temperature and MnSO₄, ZnSO₄, KJ, CuSO₄, FeSO₄ and selexen are then added in a given ratio, followed by addition of bifidobacteria. The mixture is stirred and soured until a clot of given titratable acidity is obtained.

EFFECT: improved method.

1 tbl, 1 ex

Description

The invention relates to biotechnology and can be used in the dairy industry in the production of dairy products with bifidobacteria.

A known method of growing bifidobacteria (Patent RU 2205217 C1, 2003), comprising adding to skim milk as a growth promoter of bifidobacteria serum syrup in an amount of 5.0-6.0% by weight of milk obtained by fermentation of clarified condensed serum β-galactosidase.

The disadvantage of this method of cultivation is the duration of the process, obtaining insufficiently high amounts of bifidobacteria and a significant increase in the carbohydrate component of the resulting product.

The closest to the invention in technical essence and the achieved result is a method for cultivating bifidobacteria (Patent RU 2375442 C2, 2009), where a natural mixture of oligofructose and inulin Beneo ™ Synergy 1 (BS1) is used as a stimulant for the growth of bifidobacteria when cultivated in skim milk.

The disadvantage of using this method of cultivation in an industrial environment is the high cost of a growth promoter.

The objective of the invention is the development of a method for culturing bifidobacteria in milk using a mixture of nutritionally acceptable mineral and organic compounds as a growth promoter, the cost component of which in a unit of the finished product is lower than in the analogue.

The technical result of the invention is to increase the reproductive properties of bifidobacteria when grown in milk.

The essence of the invention lies in the fact that the quality of the proposed stimulant for bifidobacteria is a mixture of mineral and organic compounds: manganese sulfate, zinc sulfate, ferrous sulfate, copper sulfate, potassium iodide and selexene (selenoxanthene).

One of the most important reasons for the stimulating effect of manganese is associated with nucleic acids, which are part of many compounds that increase enzymatic activity. Zinc stimulate the synthesis of cellular protein due to the intense incorporation of nitrogen into synthetic reactions, enhance the division process. Potassium and copper contribute to the utilization of glucose for the growth and reproduction of bacteria. Selenium is actively involved in the biochemical and physiological processes of microorganisms (in a number of sources, selenium compounds - selenates are indicated as antimetabolites. In addition, selenomethionine is one of the components of the protein of microorganisms). It is also known that bacteria containing proteins in their active center contain iron.

The strains of Bifidobacterium longum L and Bifidobacterium longum D-1 were obtained from the collection of the Museum of Pure Microorganism Culture of the Vologda State Dairy Academy named after N.V. Vereshchagin.

Microorganisms are cultured in pasteurized skim milk, to which mineral and organic compounds are added, each separately, in doses of 0.2 to 3 mg / dm ³ .

The invention is illustrated by the following examples.

Example 1

To implement the proposed method using milk subjected to pasteurization at a temperature of (87 ± 2) ° C with a holding time of (10-15) min, cooled to 37 ° C and used as a nutrient medium for the cultivation of bifidobacteria.

Strains of Bifidobacterium longum are seeded in pasteurized milk in amounts of 5% of the volume of skim milk.

Mineral and organic compounds are introduced into milk, each separately, to the required concentration, moreover, the mineral salts are pre-dissolved in distilled water and boiled, and selexene is dissolved in butter, heated to a temperature of 60-80 ° C. The fermented mixture is stirred and fermented at a temperature of 37 ° C until a clot forms (titratable acidity of 60 ° T). Control options do not contain additives of mineral and organic compounds.

Quantitative accounting of bifidobacteria was carried out by the method of limiting dilutions and sowing to standard corn-lactose medium GMK-1.

A technological assessment of the effect of a mixture of mineral and organic compounds was carried out according to the duration of fermentation and the number of bifidobacteria.

The data are given in the table.

The results obtained indicate that a mixture of mineral and organic compounds have a stimulating effect on the growth of bifidobacteria when cultured in milk at concentrations of mg / DM ³ : MnSO ₄ - (0.25 ± 0.05); ZnSO ₄ - (3 ± 0.2); KJ - (2.5 ± 0.1); CuSO ₄ - (2.0 ± 0.1), FeSO ₄ - (1.0 ± 0.1), Selexen - (0.2 ± 0.05)

The effect of a mixture of mineral and organic compounds on the growth of bifidobacteria in milk Test strains of bifidobacteria cultures Reduction of ripening time in comparison with the control,% The change in the number of bacteria compared with the control, ln CFU / cm ³ Bifidobacterium longum L 15.9 +1.41 Bifidobacterium longum D-1 16.9 +2.23

Information sources

1. RF patent No. 2205217, 2003.

2. RF patent No. 2375442, 2009.

Claims (1)

A method of cultivating bifidobacteria Bifidobacterium longum in milk, characterized in that a mixture of nutritionally acceptable mineral and organic compounds, the content of which is, mg / dm ³ , is introduced into the milk:
MnSO ₄ - (0.25 ± 0.05);
ZnSO ₄ - (3.00 ± 0.2);
KJ - (2.5 ± 0.1);
CuSO ₄ - (2 ± 0.1);
FeSO ₄ - (1 ± 0.1);
Selexen - (0.2 ± 0.05).