RU2490892C1 - GROWTH STIMULANT FOR Rhododendron L TYPES - Google Patents

Abstract

FIELD: chemistry.

SUBSTANCE: invention relates to agriculture. One of the compounds 2,2,4-trimethyl-1,2,3,4-tetrahydroquinoline and 2,2,4-trimethyl-1,2-dihydroquinoline in concentration of 0.1% is used as a growth stimulant for Rhododendron L (Ericaceae D.C. family) types.

EFFECT: invention increases stimulating effect on seedling height.

2 tbl, 1 ex

Description

The invention relates to synthetic chemical compounds of the quinoline series, which can be used as growth promoters for species of the genus Rhododendron L.

Due to the deterioration of the ecological situation in large cities of the Russian Federation, climate change and the aging of urban tree-shrub plantings, new types of plants and effective ways of stimulating their growth and development are required for landscaping. At present, various researchers are developing new stimulants for the growth and development of tree-shrub plants (Gushchina E.N., Shamanskaya L.D. Use of succinic acid as a growth stimulator in the cultivation of sea buckthorn seedlings // Achievements of Science and Technology of Agriculture. - 2009. - No. 7. - P.12-14; Korovin V.V., Payamnor V.P., Aksenov P.A.Anatomical study of the rooting process of maple cuttings during treatment with growth stimulants // Moscow State Forest University Bulletin - Lesnoy Vestnik. - 2010 . - No. 3. - S.101-107). Growth stimulants (Zircon, Epin, Fumar, Krezatsin) that increase the seed germination energy of the pine family of Far Eastern conifers (VV Ostroshenko. The effect of presowing seed treatment with growth stimulants on their sowing qualities / V.V. Ostroshenko, L.Yu. Ostroshenko // Bulletin of the Krasnoyarsk State Agrarian University. - 2011. - No. 5. - S.12.-15).

The compounds of the quinoline series, for example, dihydro- and tetrahydroquinolines, have proven themselves as stimulants of the growth and germination of seeds of woody plants, namely poplar (Shmyreva Zh.V., Rusina L.M., Frolova V.V., Alferova S.I. Rooting and growth of poplar shoots in the presence of some heterocycles // Regional Scientific Conference on Organic Chemistry: Abstracts, Lipetsk, November 26-28, 1997 - Lipetsk, 1997. - 101. Shmyreva et al., 1997; Shmyreva Zh.V. 2,2,4-trimethyl-hydroquinolines / Zh.V. Shmyreva - Voronezh: Voronezh State University, 2000.124 s.).

The seeds of the annual salvia brilliant (Salvia splendens Ker Gawl.) Varieties “Hot Fire” were tested for their germination by treatment with quinoline compounds: dihydroquinolines and tetrahydroquinolines and a number of pyrimidine-carboxylic acids (Vostrikova T.V., Devyatova T.A. and other. Growth activity of ornamental plants under the influence of chemical stimulants // Fundamental Sciences and Practice: Collection of scientific papers - Tomsk, 2010, T.1 - No. 4. - P.25-26). The most effective stimulator of seed germination from the tested compounds proved to be a stimulator from the pyrimidine-carboxylic acid group, and quinoline-type stimulants (tetrahydroquinoline and digiroquinoline) had an indicator 3-4 times lower.

In the article by I.M. Koksheeva (Koksheeva IM Optimization of the method of seed germination of representatives of the genus Rhododendron // Bulletin of the Krasnoyarsk State Agrarian University. - 2009. - No. 3. - P.80-82.) The results of experiments on optimizing the methods of seed germination for species of the genus Rhododendron L . (Ericaceae DC family). Data on the effect of the growth stimulator epin and AVA mineral fertilizer for sowing seeds are presented, their positive effect on germination, seed germination energy of representatives of the genus rhododendron is proved.

The objective of the invention is the creation of a new growth promoter for species of the genus Rhododendron L. (family Ericaceae D.C.), which is a widespread object of landscaping of urban areas.

The technical result consists in increasing the stimulating effect on the height of seedlings for species of the genus Rhododendron L. (family Ericaceae D.C.).

The technical result is achieved by the fact that as a growth promoter for species of the genus Rhododendron L. (Ericaceae DC family) one of the compounds 2,2,4-trimethyl-1,2,3,4-tetrahydroquinoline is used at a concentration of 0.01-0, 05% or 2,2,4-trimethyl-1,2-dihydroquinoline at a concentration of 0.05%.

We chose the seeds of the rhododendron Ledebur (Rhododendron ledebourii Pojark.), Which naturally grows in Altai, in Mongolia, as the object of our research. Rhododendron Ledebur is a semi-evergreen shrub up to 2 m tall, with high decorative properties. According to many years of observation in the conditions of the Botanical Garden. B.M. Kozo-Polyanskiy Voronezh State University Rhododendron Ledebur is a winter-hardy, drought-resistant and fruit-bearing plant.

To test the effect on the growth processes of Ledebour's rhododendron, we, together with the Department of Organic Chemistry of Voronezh State University, tested synthesized organic compounds of the quinoline series:

1. 2,2,4-trimethyl-1,2,3,4-tetrahydroquinoline, crystalline powder, brown (A.S. SU 548603, 1977);

2. 2,2,4-trimethyl-1,2-dihydroquinoline, a beige crystalline powder (Ivanov Yu.A. et al. Synthesis and properties of derivatives of 2,2,4-trimethyl-substituted hydrogenated quinolines and some of their analogues // Izv. USSR Academy of Sciences, Ser.Chemistry - 1981. - No. 3. - S.628-633).

- дисперсия; S_x - стандартное отклонение); на фиг.2 представлена Таблица 2. Высота проростков рододендрона Ледебура при их обработке химически синтезированными соединениями хинолинового ряда (Обозначения: * - различия с контролем достоверны (Р<0,05); ** - различия с контролем достоверны (Р<0,01); *** - различия с контролем достоверны (Р<0,001); ^а - различия дисперсий в опыте и контроле достоверны (Р<0,05); ^в - различия дисперсий в опыте и контроле достоверны (Р<0,01); ^с - различия дисперсий в опыте и контроле достоверны (Р<0,001); C_v - коэффициент вариации; $$S_x^2$$

- дисперсия; S_x - стандартное отклонение).Figure 1 presents Table 1. The strength of the effect of chemical compounds on the height of the Ledebur rhododendron seedlings (Legend: * - differences with control are significant (P <0.05); ** - differences with control are significant (P <0.01); * ** - differences with the control are significant (P <0.001); ^а - differences in the variances in the experiment and control are significant (P <0.05); ^c - differences in the variances in the experience and control are significant (P <0.01); ^s - differences the variances in the experiment and control are significant (P <0.001); C _v is the coefficient of variation; $$S_x^2$$

- variance; S _x - standard deviation); figure 2 presents Table 2. The height of the seedlings of Ledebur rhododendron when they are treated with chemically synthesized compounds of the quinoline series (Designations: * - differences with control are significant (P <0.05); ** - differences with control are significant (P <0.01 ); *** - differences with the control are significant (P <0.001); ^а - differences in the variances in the experiment and control are significant (P <0.05); ^c - differences in the variances in the experience and control are significant (P <0.01); ^c - differences in variance in the experiment and control are significant (P <0.001); C _v is the coefficient of variation; $$S_x^2$$

- variance; S _x - standard deviation).

Example

For each of the tested compounds, we took three concentrations: 0.1%, 0.05%, 0.01%. For each concentration, the experiment was carried out in triplicate of 100 seeds. The seeds were treated with 18 hours of soaking in each of the tested solutions, after which the seeds were placed in Petri dishes on filter paper. As a control, Ledebour's rhododendron seeds, soaked in tap water in triplicate, 100 seeds each, were taken. To compare the effect of the test compounds on the growth processes of Ledebur rhododendron, we used the standard stimulator epinbrassinolite (Epin-Extra, NNPP NEST M, RF). "Epin-Extra" was used in the concentration recommended by the manufacturer (0.05%). Statistical processing of the results was carried out on an IBM PC / AT type PC using the Stadia software package. Comparison of the height of the seedlings was carried out using Student's t-test. The influence of the influence factor of chemical compounds on the growth parameters of the studied plants was determined using parametric variance analysis. The influence of the factor was calculated according to the recommendations of the Snedecor (in%). The procedure for grouping data and their processing are described in the work of A.P. Kulaicheva [5].

Analysis of variance revealed the effect of chemical compounds on the height of the seedlings of rhododendron Ledebur (Fig. 1, table 1). The minimum values of the strength of influence are noted for the chemical compound 2,2,4-trimethyl-1,2-dihydroquinoline (0.2%), and the maximum for 2,2,4-trimethyl-1,2,3,4-tetrahydroquinoline (1 ,5%).

: от минимального 0,001 до максимального 0,009), что свидетельствует о выровненной реакции генотипов на действие стимуляторов. В целом, все исследуемые химические соединения оказывают стимулирующие действие на высоту проростков рододендрона Ледебура.When analyzing the data obtained, it was found that all the studied chemical compounds affect the growth processes of the Ledebur rhododendron seedlings (Fig. 2, table 2). The average height of seedlings in the control group was the smallest of all considered (0.45 ± 0.01 cm), variation of the trait according to the Lapin classification of medium degree (C _v = 11.1%). The impact of the commercial drug "Epin-Extra" was positive: 1.15 ± 0.05, with a slight coefficient of variation of 4.8%. The highest average height values of Ledebur rhododendron seedlings were noted for solutions of the following chemical compounds: 2,2,4-trimethyl-1,2-dihydroquinoline (at a concentration of 0.05%, 1.28 ± 0.01), 2.2.4 -trimethyl-1,2-dihydroquinoline (at a concentration of 0.1%, 1.24 ± 0.02), a concentration of 0.1 2,2,4-trimethyl-1,2,3,4-tetrahydroquinoline (at a concentration of 0 , 1%, 1.23 ± 0.01). The average height of the seedlings for the indicated concentrations of the compounds is higher than the height of the seedlings of the Ledebur rhododendron when treated with the Epin-Extra preparation. The lowest values of the average height of seedlings noted for the following chemical compounds, while they exceed the values in the control group: 2,2,4-trimethyl-1,2-dihydroquinoline (at a concentration of 0.05%, 0.46 ± 0.09) , 2,2,4-trimethyl-1,2-dihydroquinoline (at a concentration of 0.1%, 0.47 ± 0.01). It should be noted that the low values of the coefficient of variation and dispersion for all concentrations of chemical compounds, control and Epina-Extras (C _v : from a minimum of 2.6% to a maximum of 11.1%; $$S_x^2$$

: from a minimum of 0.001 to a maximum of 0.009), which indicates a aligned response of genotypes to the action of stimulants. In general, all the studied chemical compounds have a stimulating effect on the height of the seedlings of rhododendron Ledebur.

The obtained data on the effect of the studied chemical compounds allows us to conclude that they have a stimulating effect on growth processes. The average height of the seedlings when using three concentrations of the considered chemical compounds exceeded the control value. The height of the seedlings when using 2,2,4-trimethyl-1,2,3,4-tetrahydroquinoline (concentration of 0.1%), 2,2,4-trimethyl-1,2-dihydroquinoline (concentration of 0.05% and 0 , 1%) exceeded the height of the seedlings when using the recommended concentration of the commercial preparation "Epin-Extra".

For all concentrations of chemical compounds, control and Epina-Extra, low values of the coefficient of variation and dispersion are observed, which indicates a balanced reaction of the genotype to the action of chemical compounds that have a stimulating effect.

Chemical compounds 2,2,4-trimethyl-1,2,3,4-tetrahydroquinoline and 2,2,4-trimethyl-1,2-dihydroquinoline at a concentration of 0.1% have a stimulating effect on the height of the seedlings.

The growth and development of trees and shrubs occurs rather slowly, compared with other life forms of plants, therefore, the data obtained on the stimulating effect of synthesized chemical compounds on the germination and height of seedlings allows us to obtain a larger number of Ledebur rhododendron plants and accelerate their development.

Claims (1)

The use as a growth promoter for species of the genus Rhododendron L. (Ericaceae DC family) of one of the compounds 2,2,4-trimethyl-1,2,3,4-tetrahydroquinoline and 2,2,4-trimethyl-1,2-dihydroquinoline at a concentration of 0.1%.