RU2490008C1 - Disinfectant - Google Patents

Abstract

FIELD: medicine.

SUBSTANCE: disinfectant refers to veterinary medicine, particularly to disinfectants used for air sanitation and surface disinfection in livestock houses in the presence of animals, including poultry. The agent has an antiseptic effect, which allows using it in acute, chronic and sub-clinical mastitis, vaginitis, endometritis and other inflammatory processes of varying aetiology. An active ingredient is presented by a complex of dimethyl sulphoxide and crystalline iodine, succinic acid, fumaric and citric acids which provides better drug absorption and prolonged action. It is prepared in the form of mother liquor diluted in water immediately before use.

EFFECT: invention enables prolonging the shelf life when in use.

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Description

The invention relates to veterinary medicine, in particular to disinfectants used for sanitation of air and disinfection of surfaces of livestock buildings in the presence of animals, including birds, hatching and hatchers, as well as hatching eggs. Also, iodoprotectin has an antiseptic effect, which allows it to be used in acute, chronic and subclinical mastitis, vaginitis, endometritis and other inflammatory processes of various etiologies.

A large number of iodine-containing agents are known, which are characterized by various properties. They are used as disinfectants, antiseptics, hemostatic, irritating, distracting, anti-inflammatory and wound healing agents. [Mashkovsky M.D. Medicines - 15th ed., Rev., Rev. and add. - M: Novaya Volna Publishing House LLC, 2006. - S.701-703, 937-938.]

Means containing or releasing iodine in free form (alcohol iodine solution, iodine monochloride) have a wide range of well-defined antimicrobial (antibacterial, fungicidal, antiviral and antiprotozoal) effects. In microorganisms, such drugs, unlike antibacterial ones, do not have resistance even with prolonged use. However, they are highly toxic when introduced into the body of animals and humans, which narrows the scope of their application. They are mainly used as antiseptic and disinfectants.

At the same time, funds containing iodine in bound form, as a rule, do not have an antimicrobial effect and therefore their use for disinfection, as well as therapy and prevention of infectious and invasive diseases, makes no sense. They are mainly used as hemostatic, irritating, distracting, anti-inflammatory and wound healing agents.

Currently, iodine-containing agents are known that combine a wide range of well-defined antimicrobial effects and low toxicity to animals. This allows them to be used as disinfectants, antiseptics, hemostatic, irritating, distracting, anti-inflammatory and wound healing agents. The most famous among them are citrosol [RU 2189238 C2, 7 A61K33 / 18, A61K33 / 00, A61K31 / 00, A61P15 / 00, 06/19/2000], monclavit-1 - [http://www.monclavit.ru], saveiodim [UA 59974 A, 7 A61K9 / 08, 02.01.2003], iodine-neonol [UA 60088 A, 7 A61K33 / 18, 01.27.2003], iodine [http://www.vetlek.ru/directions/?id= 303] and others.

Monclavit-1 is known - a broad-spectrum medicinal antiseptic and disinfectant, which is an iodine-water polymer system in the form of a complex of poly-N-vinylamidacyclosulfoiodide. Monclavit-1 has a wide range of pronounced antimicrobial activity and low toxicity, which allows it to be used for:

- effective treatment and prevention of infectious diseases of animals;

- prevention and treatment of iodine-deficient animal diseases;

- increase non-specific immunity, stimulate growth;

- sanitation of the air in the presence of animals;

- disinfection of livestock buildings, hatching and hatchers, hatching eggs;

- Improving the hygiene of milk.

The use of monclavite-1 can reduce the cost of treatment and prevention of infectious diseases, increase production efficiency and improve the quality of livestock products according to the main indicators. However, it has a relatively short shelf life.

Saveyodim is an anti-inflammatory and antiseptic drug based on a complex of dimethyl sulfoxide with crystalline iodine. Designed for washing and disinfecting milking equipment and other milk containers while maintaining sanitary standards for milk quality. It can also be used in acute, chronic and subclinical mastitis, vaginitis, endometritis, retention of the placenta, inflammatory processes and lesions of various etiologies. This solution is the closest analogue and can be used as a prototype.

The objective of the invention is the development of an antiseptic, anti-inflammatory and disinfectant for sanitation of air and disinfection of the surfaces of livestock buildings in the presence of animals, including birds, hatching and hatchers and hatching eggs with a long shelf life; expansion of the product range. The proposed tool has an antiseptic effect, which allows it to be used in acute, chronic and subclinical mastitis, vaginitis, endometritis and other inflammatory processes of various etiologies.

For this, a complex of dimethyl sulfoxide with crystalline iodine, succinic, fumaric and citric acids was used as an active substance in the iodoprotectin disinfectant. This complex provides better absorption of the drug and prolonging effect. In addition, a distinctive feature of the iodoprotectin disinfectant is that it is released in the form of a mother liquor, diluted with water immediately before use. This can significantly increase its shelf life, since iodine in concentrated form is less susceptible to oxidation.

The proposed disinfectant iodoprotectin contains crystalline iodine, dimethyl sulfoxide, sodium phosphate disubstituted, potassium iodide, ethyl alcohol, succinic acid, citric acid, fumaric acid, glycerin, savenol and distilled water in the following ratio of components, wt.%:

crystalline iodine 5,200-5,400 dimethyl sulfoxide 5,200-5,400 disubstituted sodium phosphate 0,800-1,200 potassium iodide 0.005-0.010 ethanol 0.005-0.100 succinic acid 0,005-0,200 lemon acid 0.005-0.100 fumaric acid 0.005-0.100 glycerol 0,100-0,200 savenol 32,000-35,000 distilled water rest.

Comparison of the disinfectant iodoprotectin with the drug saveiodim allows us to conclude that the proposed tool differs from the indicated by the presence of new components (succinic, citric and fumaric acids, potassium iodide, glycerol) and the mass percentage of the same components. These differences make iodoprotectin a more effective disinfectant than saveiodim.

Case Studies

In the course of the research, the iodoprotectin disinfectant was used with the following ratio of its constituent components, wt.%:

crystalline iodine 5.20 dimethyl sulfoxide 5.20 disubstituted sodium phosphate 2.00 potassium iodide 0.01 ethanol 0.10 succinic acid 0.20 lemon acid 0.10 fumaric acid 0.10 glycerol 0.20 savenol 35.00 distilled water rest.

Experience 1. Conducted a study on the effectiveness of the use of iodoprotectin disinfectant in the direction of sanitation of air in the presence of birds. In the experiment, 4 houses were taken. Another 4 houses served as a control, where air sanitation was carried out with iodine monochloride. The treatment was carried out at the age of the bird 3, 4, 8, 9, 13, 14, 19 and 20 days, i.e. between vaccinations.

A working solution of iodoprotectin disinfectant was prepared by adding ordinary water to its mother liquor in a ratio of 1:99. Aerosol spraying was carried out using jet spray cold fog generators at the rate of 3.0 ml / m ³ according to the scheme: 10-15 minutes spraying and 30 minutes exposure. The total number of birds in experimental houses on the 20th day of the study was 260,304 goals.

Air sanitation in control houses was carried out with iodine monochloride by exothermic sublimation of iodaluminium and chloraluminium vapors obtained from the combination of iodine monochloride with aluminum. The consumption of iodine monochloride was 3 ml / m ³ . Exposure from the start of the steam reaction is 35-37 minutes. The total number of birds in the control houses on day 20 of the study was 258,957 goals.

As a result of the studies, it was found that:

- studies on the intensity of post-vaccination immunity against Newcastle disease, infectious bursal disease, and chicken infectious bronchitis did not reveal a negative effect of iodoprotectin on the level and uniformity of antibody titer;

- After vaccination, no complications in the form of second infections (colibacillosis) were noted, which indicates a decrease in the bacterial background in the house. This has reduced the use of antibacterial drugs;

- studies of poultry blood serum for non-specific resistance revealed an increase in the level of lysozyme activity by an average of 30.2%, bactericidal activity - by 13.0%.

Experience 2. Conducted a study on the effectiveness of the use of iodoprotectin disinfectant in the direction of sanitation of pig air in the presence of pigs.

A working solution of iodoprotectin disinfectant was prepared by adding ordinary water to its mother liquor in a ratio of 1:99. Aerosol spraying was carried out using jet spray cold fog generators at the rate of 5.0 ml / m ³ according to the scheme: 10-15 minutes spraying and 45 minutes exposure. The total number of pigs in the pigsty is 307 goals.

To control the quality of aerosol disinfection, Petri dishes with sterile Endo and Chapek media were placed diagonally at 6 different points. The microbiological contamination of the air of the pigsty was determined by the sedimentation method immediately before aerosol disinfection, 5 and 45 minutes after aerosol disinfection by counting the grown colonies on the indicated media of colonies of microorganisms after incubation in a thermostat (for Endo medium - 1 day at 37 ° C, for Chapek's medium - 3 days at a temperature of 26 ° C). The results of assessing the quality of air sanitation with a disinfectant iodoprotectin are presented in table 1.

Table 1. The quality of aerosol disinfection of indoor air in the presence of pigs Before aerosol disinfection After aerosol disinfection after 5 minutes after 45 minutes Sample number Wednesday Endo Chapek Wednesday Sample number Wednesday Endo Chapek Wednesday Sample number Wednesday Endo Chapek Wednesday 1-1 eleven 25 1-2 four 10 1-3 one 6 2-1 eighteen 9 2-2 8 four 2-3 2 3 3-1 fourteen 10 3-2 5 four 3-3 2 2 4-1 17 23 4-2 eleven 8 4-3 3 3 5-1 fifteen 19 5-2 7 9 5-3 2 four 6-1 16 13 6-2 13 6 6-3 3 3 Average

As a result of the studies, it was found that the disinfectant iodoprotectin:

- can be successfully used for sanitation of indoor air in the presence of pigs;

- harmless to animals, as does not have any negative effect on them (increased breathing, changes in behavioral reactions, irritation of the mucous membranes or skin, etc.).

Experience 3. Conducted a study of the effectiveness of iodoprotectin as an antiseptic in acute, subacute and chronic endometritis of cattle in the dairy industry. In total, 360 heads of cows and heifers with various forms of endometritis were taken into the experiment.

The experimental design for the study of the antiseptic properties of iodoprotectin in acute endometritis is shown in Table 2. The animals of the experimental groups were given funds for acute endometritis intrauterine one to two times a day. The animals of the first experimental group (n = 30) were injected with an iodoprotectin at a dilution of 1:20 (diluted with distilled water) at a dose of 100-150 ml for antiseptic treatment. The animals of the second experimental group (n = 30) were injected with monclavit-1 at a dose of 150-200 ml. The animals of the third experimental group (n = 30) were injected with a ready-made solution of saveiodim in a dose of 100-150 ml. The control group of animals (n = 30) was not subjected to processing. These activities in the experimental groups were carried out for 2-3 days before clinical recovery. Before using these funds, the uterus was stimulated by the administration of oxytocin preparations.

According to the same scheme (Tables 2 and 3), groups of cows of patients with subacute and acute forms of mastitis were formed, which were treated with iodoprotectin, saveiodim and monoclovitis-1 in the same way. The duration of the experiment in different groups was 2-3 days.

Table 2. Scheme of conducting an experiment on the study of the antiseptic properties of iodoprotectin in comparison with analogues in acute endometritis Group of animals (No.) and their number (n) The tool used for processing Recovered Goal. 96 No. 1 (n = 30) Iodoprotectin 29th 73 No. 2 (n = 30) Monclavit 1 22 86 No. 3 (n = 30) Saveyodim 26 0 No. 4 (n = 30) - 0 96

Table 3. Scheme of conducting an experiment on the study of the antiseptic properties of iodoprotectin in comparison with analogues in subacute endometritis Group of animals (No.) and their number (n) The tool used for processing Recovered Goal. % No. 1 (n = 30) Iodoprotectin thirty one hundred Group of animals (No.) and their number (n) The tool used for processing Recovered Goal. % No. 2 (n = 30) Monclavit 1 24 80 No. 3 (n = 30) Saveyodim 27 90 No. 4 (n = 30) - 0 0

Table 4. Scheme of conducting an experiment on the study of the antiseptic properties of iodoprotectin in comparison with analogues in chronic endometritis Group of animals (No.) and their number (n) The tool used for processing Recovered Goal. % No. 1 (n = 30) Iodoprotectin 28 93 No. 2 (n = 30) Monclavit 1 21 70 No. 3 (n = 30) Saveyodim 23 76 No. 4 (n = 30) - 0 0

As a result of the studies, the high efficiency of the use of iodoprotectin as an antiseptic was established in comparison with analogues. The treatment efficiency for various forms of endometritis in cows and heifers with iodoprotectin was 93-100%, while Savedime - 76-90% and Monclavite-1 - 70-80%.

Experience 4. Conducted a study of the effectiveness of iodoprotectin as an antiseptic in acute, subacute and chronic mastitis in cattle of the dairy farm. In total, 360 heads of cows and heifers with various forms of mastitis were taken into the experiment.

Prior to the experiment, the number of somatic cells in the milk of diseased cows and heifers was checked using the California mastitis test (DeLaval company). The number of somatic cells in all groups ranged from 1.5 to 2.0 million / ml.

The experimental design for the study of the antiseptic properties of iodoprotectin in acute mastitis is shown in Table 5. The animals of the experimental groups were injected with acute mastitis intracisternally twice a day, after morning and evening milking, and the surface of the nipple skin was treated with these agents. Animals of the first experimental group (n = 30) were injected with iodoprotectin in a dose of 10-15 ml for the purpose of antiseptic treatment. The animals of the second experimental group (n = 30) were injected with monclavit-1 at a dose of 10-20 ml. Animals of the third experimental group (n = 30) were injected with saveiodim at a dose of 10-15 ml. The control group of animals (n = 30) was not subjected to processing. These activities in the experimental groups were carried out for 7-10 days before clinical recovery.

According to the same scheme (Tables 6 and 7), groups of cows of patients with subacute and acute forms of mastitis were formed, which were treated with iodoprotectin, Saveyodim and monoclovitis-1 in the same way. The duration of the experiment was 6-12 days.

Table 5. Scheme of conducting an experiment on the study of the antiseptic properties of iodoprotectin in comparison with analogues in acute mastitis Groups of animals (No.) and their number (n) The tool used for processing Recovered Goal. % No. 1 (n = 30) Iodoprotectin 28 93 No. 2 (n = 30) Monclavit 1 22 73 No. 3 (n = 30) Saveyodim 24 80 No. 4 (n = 30) - 0 0

Table 6. Scheme of conducting an experiment on the study of the antiseptic properties of iodoprotectin in comparison with analogues in subacute mastitis Groups of animals (No.) and their number (n) The tool used for processing Recovered Goal. % No. 1 (n = 30) Iodoprotectin thirty one hundred No. 2 (n = 30) Monclavit 1 25 83 No. 3 (n = 30) Saveyodim 28 93 No. 4 (n = 30) - 0 0

Table 7. Scheme of conducting an experiment on the study of the antiseptic properties of iodoprotectin in comparison with analogues in acute mastitis Groups of animals (No.) and their number (n) The tool used for processing Recovered Goal. % No. 1 (n = 30) Iodoprotectin 28 93 No. 2 (n = 30) Monclavit 1 21 70 No. 3 (n = 30) Saveyodim 25 83 No. 4 (n = 30) - 0 0

After clinical recovery, the number of somatic cells in the milk of cows and heifers was checked using a California mastitis test (DeLaval). The number of somatic cells in the first experimental group was 150-180 thousand / ml, in the second experimental - 220-230 thousand / ml, in the third experimental - 200-220 thousand / ml. Sowing milk samples from cows and first-calf heifers of the first and second experimental groups on meat-peptone agar and meat-peptone broth did not give growth.

As a result of the studies, the high efficiency of the use of iodoprotectin as an antiseptic was established in comparison with analogues. The treatment efficiency of cows and heifers with various forms of endometritis with iodoprotectin amounted to 93-100%, while save-dime - 80-93% and monclavite-1 - 70-83%.

Experience 5. Study of the effectiveness of the use of iodoprotectin disinfectant for disinfection of the shell of hatching eggs and the surface of hatching cabinets in the incubator. In the experiment, an incubation egg (a total of 21,000 pieces) of broiler cross "F-15" was used. A control batch of eggs (7000 pieces) was treated with formaldehyde vapors according to the scheme adopted in the incubator. The first experimental batch of eggs (7000 pieces) was treated with iodoprotectin, the second (7000 pieces) was treated with monclavite-1 according to the manufacturer's instructions.

Before laying the eggs in the incubator, the eggs were treated by irrigating the surface of the shell with iodoprotectin from the Gloria sprayer. Consumption was 10 liters per 7000 eggs. Before laying eggs, the inner surface of the incubation cabinets (Universal-45) was irrigated using a Gloria sprayer with iodoprotectin at the rate of 1 liter per 30 m ^{2 of the} inner surface of the cabinet with an exposure time of 10 minutes.

In a similar manner, hatching eggs and cabinets were treated with monclavite-1.

The quality of disinfection of the eggshell and the inner surface of the incubation cabinets was controlled by the presence / absence of microflora growth from the washings. Flushing was done immediately before disinfection and 30 and 60 minutes after it.

The temperature and humidity regime of incubation corresponded to zoohygienic and veterinary sanitary standards.

Re-disinfected the inner surface of the cabinet and the eggshell on the twelfth day of incubation. Disinfection was carried out using a compressor and a spray through the incubator flap at a rate of 280 ml of iodoprotectin per incubation cabinet with an exposure of 10 minutes. Monclavite-1 was disinfected in a similar manner in the second experimental batch. The lack of microflora growth from swabs confirmed the quality of re-disinfection in all three batches of eggs.

The hatching of chickens in the first experimental, second experimental and control groups occurred at the standard time. In the first experimental group, hatchability was 89.3%, in the second - 88.6% and in the control - 88.1%.

Studies have established that:

- the hatchability when using iodoprotectin exceeded the same indicator when using formalin according to the scheme adopted at the incubator by 1.2%, and when using monclavit-1 - by 0.7%;

- the quality of the disinfection of eggshells and incubation cabinets with iodoprotectin complies with veterinary and sanitary standards.

Thus, the disinfectant iodoprotectin has the following advantages compared to analogues:

- longer shelf life (4 years);

- available in the form of a concentrated mother liquor, which facilitates its transportation and storage;

- the preparation of the working solution does not cause difficulties;

- more effective for sanitation of the air in the presence of animals;

- effective for disinfection of livestock buildings, hatching and hatchers, hatching eggs;

- helps to improve the hygiene of milk;

- a more pronounced effect affecting the level of growth of indicators of nonspecific immunity.

Claims (1)

A disinfectant, including crystalline iodine, dimethyl sulfoxide, sodium phosphate disubstituted, potassium iodide, ethyl alcohol, succinic acid, citric acid, fumaric acid, glycerin, savenol and distilled water in the following ratio, wt.%:
crystalline iodine 5,200-5,400 dimethyl sulfoxide 5,200-5,400 disubstituted sodium phosphate 0,800-1,200 potassium iodide 0.005-0.010 ethanol 0.005-0.100 succinic acid 0.005-0.200 lemon acid 0.005-0.100 fumaric acid 0.005-0.100 glycerol 0,100-0,200 savenol 32,000-35,000 distilled water rest