RU2485788C2 - METHOD FOR PRODUCTION OF NUTRITIONAL MEDIUM FOR CULTURE OF LARVAE OF Cryptolaemus montrouzieri Muls PREDACIOUS BEETLE - Google Patents

Abstract

FIELD: food industry.

SUBSTANCE: invention relates to biotechnology and may be used for useful insects culture. The method envisages preset sequence mixing of soya flour, powdered milk, maize oil, palm kernel oil, sucrose, a mixture of mineral salts, agar agar, dry scales, beer yeast autolysate, inositol, tocopherol, ascorbic acid, vitamins B1, B6 and B12, metaben and distilled water taken at a preset ratio.

EFFECT: invention allows to decrease the period of nutritional medium preparation and reduce the production energy intensity.

Description

The invention relates to feed production technology, in particular to methods for producing feed for breeding beneficial insects.

A known method of producing a nutrient medium for breeding cryptolemus beetle larvae of Cryptolaemus montrouzieri Muls., Comprising dosing and mixing casein, lysine, arginine, histidine, glutamic acid, cystine, glycine, corn oil, palm kernel oil (coconut or cream, sugar) mixtures of mineral salts, agar-agar, dried worms, brewer's yeast autolysate, inositol, para-aminobenzoic acid, tocopherol, calcium pantothenate, ascorbic acid, nicotinic acid, choline chloride, bioti and B12, folic acid, sorbic acid and distilled water in the following ratio, g per 100 g of the medium:

casein 14.0 lysine 0.015 arginine 0.015 histidine 0.01 glutamic acid 0.05 cystine 0.15 glycine 0.15 corn oil 1,0 palm kernel oil (coconut or cream) 2,5 cholesterol 0.1 sucrose 13.0 a mixture of mineral salts 1,5 agar agar 1,0 dry worm 20,0 brewer's yeast autolysate 20,0 inositol 0.02 para-aminobenzoic acid 0.02 tocopherol 0.06 calcium pantothenate 0,03 vitamin C 0.15 a nicotinic acid 0,03 choline chloride 0.08 biotin 0,0004 vitamin b12 0.00006 folic acid 0.015 sorbic acid 0.12 distilled water 30,0

(Guidelines for the cultivation of the predatory beetle of the cryptolemus Cryptolaemus montrouzieri Muls. On artificial nutrient media. - Leningrad. - 1974).

The disadvantage of this method is the high consumption of expensive and time-consuming components, the duration of the preparation and the energy intensity.

The technical result of the invention is to reduce the cost of expensive and time-consuming components in the manufacture, preparation time of the nutrient medium and energy consumption.

This result is achieved in that in a method for producing a nutrient medium for breeding cryptolemus beetle larvae of Cryptolaemus montrouzieri Muls., Dosing and mixing corn oil, palm kernel oil, sucrose, a mixture of mineral salts, agar agar, dry worm, brewer's yeast autolysate, inositol , tocopherol, ascorbic acid, vitamin B12 and distilled water according to the invention, soy flour, milk powder, vitamins B1 and B6 and metaben are additionally introduced into the mixture in the following ratio of components, .% Al:

soy flour 10.0 powdered milk 2.0 corn oil 1,0 palm kernel oil 2,5 sucrose 10.0 a mixture of mineral salts 1,5 agar agar 2.0 dry worm 3.0 brewer's yeast autolysate 4.0 inositol 0.002 tocopherol 0.06 vitamin C 0.2 vitamin b1 0.006 vitamin b6 0.006 vitamin b12 0,0002 metaben 0.2 distilled water rest.

The method is implemented as follows.

Soy flour is hydrolyzed for one hour in a water bath. Then sucrose, a mixture of mineral salts, milk powder, melted agar-agar are gradually introduced. After slight cooling, add oils, autolysate of brewer's yeast, ground dried worms, inositol, ascorbic acid, tocopherol, vitamins and metaben. The listed components are dosed and mixed in the specified ratio. The preparation time of the nutrient medium is reduced, in comparison with the closest analogue, from five to two hours due to the replacement of casein with soy flour (casein is hydrolyzed for 5 hours). This simplifies the technology of production of the nutrient medium and reduces its energy consumption. The medium is poured into Petri dishes and stored in the refrigerator at a temperature of 4-6 ° C. The medium has a jelly-like consistency and is convenient to use.

A comparative analysis of the formulation of the target product according to the content of components for the proposed method and the closest analogue showed that the consumption of sucrose was reduced by 23% and brewer's yeast by 80%, animal protein (casein) was replaced by cheaper and more technologically advanced components - soy flour ( 10 g) and milk powder (2 g). The consumption of dry worms is also significantly reduced - by 85%, the cultivation and collection of which is an expensive and time-consuming process, and the drying of worms requires significant energy consumption. In this case, it was experimentally established that a change in the recipe composition of the nutrient medium according to the invention does not worsen the conditions and results of breeding larvae of the predatory beetle cryptolemus. As a result of breeding on the proposed nutrient medium, 95% of the larvae pupate, the flight of adults is 85%, which corresponds to the indices of the closest analogue.

Thus, the proposed method can reduce the cost of expensive and time-consuming components to manufacture, as well as significantly reduce its preparation time and reduce the energy consumption of production.

Claims (1)

A method for the production of a nutrient medium for breeding the cryptolemus beetle larvae Cryptolaemus montrouzieri Muls., Comprising dosing and mixing corn oil, palm kernel oil, sucrose, a mixture of mineral salts, agar-agar, dry worms, autolysate of brewer's yeast, inositol, tocopherol, vitamin A, B12 and distilled water, characterized in that it additionally uses soy flour, milk powder, vitamins B1 and B6 and metaben in the following ratio of components, wt.%:
soy flour 10.0 powdered milk 2.0 corn oil 1,0 palm kernel oil 2.5 sucrose 10.0 a mixture of mineral salts 1,5 agar agar 2.0 dry worms 3.0 brewer's yeast autolysate 4.0 inositol 0.002 tocopherol 0.06 vitamin C 0.2 vitamin b1 0.006 vitamin b6 0.006 vitamin b12 0,0002 metaben 0.2 distilled water rest