RU2468608C1 - Method for production of biologically active selenium-containing wheat additive - Google Patents

Abstract

FIELD: food industry.

SUBSTANCE: invention relates to food industry and may be used for prevention and adjuvant therapy of diseases caused by selenium deficit. The method involves separation of a selenium-containing amino acid component preferably - with selenomethionine) from the vegetal raw material by way of water-fermentative hydrolysis with Protosubtilin and Celloviridin fermentative preparations with subsequent centrifugation or filtration of the hydrolysate and its drying till powdery condition. The raw material is represented by flour of soft spring wheat enriched with selenium. Hydrolysis is performed under periodical stirring conditions.

EFFECT: method implementation allows to produce a complex of indispensable amino acids and selenium, preferably, in the form of organic compounds with high digestibility and low toxicity as well as maximally separate selenium organic compounds from wheat.

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Description

The invention relates to a method for producing a biologically active additive based on plant materials of soft spring wheat and can be used in the food industry for the prevention and adjunctive therapy of diseases caused by selenodeficiency.

Currently, as a result of lengthy research conducted by scientists around the world, selenium is classified as an indispensable (essential) food substance, the receipt of an adequate amount of which is a necessary condition for ensuring normal human life. The lack of this trace element in the human body not only reduces immunity and performance, but also leads to the development of cardiovascular (one of the most common diseases is Keshan’s disease, accompanied by severe dystrophic damage to the heart) and cancer, the accumulation of heavy metals and premature aging, atherosclerosis and other diseases.

Selenium atoms act as catalysts for redox reactions. Selenium is part of glutathione peroxidase, which is involved in the inhibition of free radical oxidation reactions. Therefore, the production of biologically active additives enriched with selenium is relevant.

A known method of producing a biologically active additive from chickpea (RF Patent No. 2210260, class A23L 1/30, A23L 1/305, publ. 08/20/2003). The method involves the germination and fermentation of chickpea seeds in melt water until the seeds contain a sum of aspartic and glutamic acids of at least 35% of the amount of amino acids, and selenium - 22 μg / 100 g, after inactivation of the enzymes, milk thistle seeds and aminoacetic acid are added to the resulting mass. glycine in amounts of 10-20% and not less than 0.03%, respectively. The invention allows to expand the range of biologically active additives of prophylactic action that regulate metabolism and increase the antitumor resistance of the body.

The disadvantage of this method is the expensive raw materials, the multi-stage and complexity of the technology, as well as the low content of selenium in the biologically active additive.

The closest in technical essence is a method for producing glucose-maltose-amino acid feed additives from grain cereals of wheat and rye. The invention relates to feed production. The method includes grinding the raw materials and enzymatic hydrolysis in a rotary pulsation dispersant, while the crushed raw materials are mixed with electroactivated water to form a suspension, the suspension is diluted with amylolytic enzymes, then proteolytic enzymes are added. The temperature of the hydrolyzate is maintained at a level of 60-65 ° C, pH 4.5-5.0. The method allows to obtain a product containing up to 20-30% easily digestible carbohydrates and a complex of essential amino acids (Patent No. 2346461, class A23K 1/100, A23K 1/16, publ. 02.20.2009).

The disadvantage of this method is that it contains free amino acids - a secondary product, in addition, the implementation of this method requires expensive equipment.

The task of the technical solution is the selection of the amino acid component, which includes organic selenium (selenomethionine) from wheat with a high trace element content.

The technical result is achieved by using soft spring wheat grain grown with the addition of selenium-containing fertilizer and extracting the amino acid component from it with the help of the enzyme preparations Celloviridin G20X and Protosubtilin GZH as a raw material for producing a biologically active additive.

A method of obtaining a biologically active additive (BAA) from soft spring wheat with a high content of selenium is implemented as follows: preparation of wheat flour with a particle size of 0.2-1.5 mm; water-enzymatic hydrolysis of flour carried out using enzymatic preparations with a proteolytic effect (for maximum extraction of free amino acids from raw materials) Protosubtilin GZH in an amount of 0.5 ± 0.002% of the dry weight of the raw material and cellulolytic action (for the destruction of the external structural elements of tissues and cells and facilitate process of extraction of amino acids) Celloviridin G20X in an amount of 0.1 ± 0.001% of the dry weight of the raw material, at pH 6.0, temperature + 45 ± 1 ° C (average optimum action of the complex of enzyme preparations) for 3 hours at iodicheskom stirring every 30 min .; centrifugation or filtration and drying of the hydrolyzate in a spray dryer at a temperature of + 95 ± 1 ° C for 1-2 seconds, since the short-term exposure to this temperature to a lesser extent destroys the selenium compounds to a powder state of the hydrolyzate with a mass fraction of moisture of 1.5-2%; packaging and labeling of dietary supplements; storage and transportation of dietary supplements.

When changing the hydrolysis conditions: temperature (for example, + 24 ° С), time (for example, 3, 4 and 5 hours), dosage of the enzyme preparations Protosubtilin and Celloviridine (for example, 0.1% and 0.1% or 0.5 and 0.5% of dry weight, respectively), the use of other enzyme preparations (for example, the complex of enzyme preparations Pepsin and Celloviridin), as well as changes in the duration and temperature of drying (higher temperature or longer exposure lead to intensive destruction of selenium compounds) - the output of dietary supplements as well as organic villages yen was less than with the totality of the features of the proposed method. Filtration or centrifugation is necessary to separate the liquid hydrolyzate that will be dried.

The specific implementation of the method:

Example 1. To obtain 1 kg dietary supplement grain enriched with selenium soft spring wheat is ground into flour to a particle size of 0.2-1.5 mm The grain must be used completely, together with the shells, due to the fact that they contain ash substances. Enzyme preparations Protosubtilin GZH, in an amount of 0.5 ± 0.002% of the dry weight of flour and Celloviridin G20X, in an amount of 0.1 ± 0.001% of the dry weight of flour, previously dissolved in water with a temperature of + 45 ± 1 ° C, take a five-fold volume water from the taken flour and then add 10 kg of flour with constant stirring. Hydrolysis is carried out at pH 6.0, temperature + 45 ± 1 ° C for 3 hours with periodic stirring with an interval of 30 minutes Samples are centrifuged for 5 minutes at a speed of 5000 rpm and dried in a spray dryer at a temperature of + 95 ± 1 ° C for 1-2 seconds to a powder state with a mass fraction of moisture of the hydrolyzate of 1.5-2%. Supplements are Packed 1 kg in sealed opaque plastic bags.

The technical result is a method for the maximum extraction of the organic form of selenium from wheat enriched with this trace element. This method allows to obtain dietary supplements with a selenium content of up to 110 μg / 100 g. The amount of selenium transferred to the hydrolyzate from flour is 55%.

Example 2. This method is completely similar to the first, with the difference that the hydrolyzate is filtered and not centrifuged. The technical result of this method is also the maximum extraction of the organic form of selenium from wheat enriched with this trace element. Using this method, it was possible to obtain dietary supplements with a selenium content of up to 110 μg / 100 g. The amount of selenium transferred to the hydrolyzate from flour is 55%.

The invention allows to expand the range of dietary supplements and food products containing selenium in a less toxic and more digestible organic form, as a result of which the biologically active additive can be used as an enriching additive for food products.

Information sources

1. Patent No. 2346461, cl. A23K 1/100, A23K 1/16, publ. 02/20/2009 - a prototype.

2. RF patent No. 2210260, cl. A23L 1/30, A23L 1/305, publ. 08/20/2003.

Claims (1)

A method of obtaining selenium-containing biologically active additives from wheat, characterized in that flour is prepared from wheat enriched with selenium, water-enzymatic hydrolysis is carried out using enzyme preparations Protosubtilin in an amount of 0.5 ± 0.002% by weight of dry raw materials and Celloviridine in an amount of 0.1 ± 0.001% by weight of dry raw materials to isolate the amino acid component with selenium in organic form, mainly selenomethionine, at pH 6.0, temperature + 45 ± 1 ° С for 3 hours with periodic stirring with an interval of 30 minutes, then carried out centrifugation or filtration of the hydrolyzate, the hydrolyzate was dried at + 1-2 95 ± 1 ° C to a powder with a mass fraction of moisture of 1.5-2%.