RU2443783C2 - Method for production of feed hydrolysates of wheat grains - Google Patents

Abstract

FIELD: food industry.

SUBSTANCE: method envisages production of a water-and-grain suspension in a cavitation disperser of flattened wheat grains and acid water solution with pH equal to 4.0-4.5 units which solution is produced by addition of formic or citric acid. In the cavitation disperser flattened grains undergo disintegration and disagglomeration in water medium with simultaneous self-heating during 15-20 minutes. Cereal starch gelatinisation is performed at a temperature of 60-90°C, dilution - at 60-85°C with multi-enzymic compositions, saccharification is performed at a temperature of 60-70°C to produce the ready product. For dilution one uses multi-enzymic compositions consisting of amylosubtiline and CelloLux or Liquozyme Supra and Accellerase 1000, for saccharification -glucolux or Dextrozyme DX with calculated activity in one cavitation apparatus.

EFFECT: simplification and acceleration of hydrolysis of starch-containing raw material, reduction of energy consumption and decrease of enzymic preparations quantity.

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Description

The invention relates to the agro-industrial complex, namely to animal husbandry and fodder production, and provides for the enzymatic hydrolysis of wheat grains to produce feed hydrolysates (molasses) containing products of deep starch hydrolysis: glucose and maltose.

Known methods for producing feed molasses from wheat grain [1, 2]. The closest analogue of the proposed method is a method for producing fodder molasses from crushed grain and its enzymatic hydrolysis at a grain: water ratio of 1: 1, when after supplying half the grain to the cavitator add enzymes: α-amylase in an amount of 1.0-1.5 units. / 1 g of starch and xylanase 1-2 units / 1 g of cellulose. The process is carried out at 43-50 ° C, pH 6.2-6.4 with the addition of hydrochloric acid or soda ash. After 30-40 minutes, cavitation reaches a temperature of 62-65 ° C and can withstand 30 minutes without cavitation. The end of the cavitation process is determined by the iodine test, after which the reaction mass is transferred to another larger tank with a saccharification agitator in the presence of glucavamorin G3x in an amount of 3 units. / 1 g of starch. The saccharification process is carried out at 55-58 ° C, pH 5.5-6.0 for 4-6 hours and then concentrated in vacuo to a concentration of solids of 40-45% [2].

The objective of the present invention is the exclusion from the technological cycle of strong mineral acids that degrade the quality of the finished product, increasing the completeness of bioconversion of starch-containing raw materials, reducing the duration of the process, reducing the number of processing equipment.

The solution of the problem of the present invention is achieved by the fact that:

- exclude strong mineral acids from the technological cycle, replacing them with organic acids: formic or citric;

- reduce the duration of technological operations due to the intensification of the process of enzymatic hydrolysis of grain starch by carrying out hydrolysis in a cavitation apparatus DKD-PM-22 pre-processed by the method of crushing wheat grain by crushing.

The method is as follows. The calculated amount of formic or citric acid is added to the water pre-purified at the filtration unit to a pH of 4.0-4.5 units and mixed in a cavitation apparatus for 3-5 minutes at a temperature of 18-40 ° C. Then, the rolled grain is uniformly fed into the cavitation apparatus and the gelatinization stage is carried out by self-heating the water-grain suspension during cavitation treatment at 60-90 ° C for 15-20 minutes. Liquefaction of the grain suspension is carried out at a temperature of 60-85 ° C under the action of multi-enzyme composition I (IEC-1), including amylosubtiline with an activity of 1300 EA at the rate of 0.3-0.5 units / g of starch and celllux with an activity of 2000 EA at the rate of 0 , 5-1.0 units / g of cellulose or multi-enzyme composition II (IEC-II), consisting of Liquozyme Supra with an activity of 135 EA / g and Accellerase 1000 with an activity of 2932 EA / G. The duration of the liquefaction stage in the cavitation apparatus is 5-10 minutes. Saccharification is carried out in the same cavitation apparatus under the influence of glucalux with an activity of 5000 EA based on 2-3 units / g of starch or Dextrozyme DX with an activity of 170 EA / g, the duration of saccharification is 2-3 hours at a temperature of 60-70 ° C.

Get feed hydrolyzate containing solids of 29-34% with a total sugar content of 21-24%.

Example 1. In pre-purified water weighing 100 kg with a working cavitation apparatus, formic acid is added to achieve a pH of 4.0-4.5 units and 50 kg of rolled grain are added with constant stirring for 15-20 minutes. When cavitation is applied to the grain, its disintegration, heating and gelatinization of the reaction mixture occur at 60-90 ° С. Next, the water-grain suspension is cooled to 60-85 ° C and make MEK-I, consisting of 20 g of amylosubtiline with an activity of 1300 EA and 2.5 g of cellulux with an activity of 2000 EA. The liquefaction stage is carried out for 5-10 minutes, after which 15 g of glucalux with an activity of 5000 EA are added and saccharification is carried out in a cavitation apparatus for 2-3 hours at a temperature of 60-70 ° C.

A feed hydrolyzate is obtained having the following composition: humidity 69.0 ± 2.0%, total sugars 22 ± 1.0%, protein 3.7 ± 0.2%, fats 0.5 ± 0.1%.

Example 2. In pre-treated water weighing 100 kg with a working cavitation apparatus, citric acid is added until a pH of 4.0-4.5 units is reached and 50 kg of rolled grain are added with constant stirring for 15-20 minutes. In this case, the apparatus is grinding grain, disintegrating protein-starch agglomerates with simultaneous heating and further gelatinization of the reaction mass at 60-90 ° C. After that, the water-grain suspension is cooled to 60-85 ° C and make IEC-I, consisting of 20 g of amylosubtiline with an activity of 1300 EA and 2.5 g of cellulux with an activity of 2000 EA. The liquefaction stage is carried out for 5-7 minutes, after which 15 g of glucalux with an activity of 5000 EA are added and saccharification is carried out in a cavitation apparatus for 3 hours at a temperature of 60-70 ° C.

A feed hydrolyzate is obtained having the following composition: humidity 69.0 ± 2.0%, total sugars 22 ± 1.0%, protein 3.7 ± 0.2%, fats 0.5 ± 0.1%.

Example 3. Formulated acid is added to purified water weighing 100 kg with a working cavitation apparatus until a pH of 4.5-5.5 is reached and 50 kg of rolled grain are added with constant stirring for 15-20 minutes. In the apparatus during cavitation exposure to grain, it undergoes disintegration - disagglomeration and heating to 60-90 ° C, at which the gelatinization of grain starch occurs. Next, the water-grain suspension is cooled to 60-85 ° C and make IEC-II, consisting of 30 g of Liquozyme Supra with an activity of 135 EA / g and 2.0 g of Accellerase 1000 with an activity of 2932 EA / g to dilute the reaction mass, which proceeds within 5-7 minutes. After that, the temperature of the reaction mixture was brought to 60-70 ° C and the saccharification step was carried out in the same cavitation apparatus in the presence of 25 g of the enzyme preparation Dextrozyme DX with an activity of 170 EA / g.

A feed hydrolyzate is obtained having the following composition: humidity 68.0 ± 2.0%, total sugars 23 ± 1.0%, protein 3.8 ± 0.2%, fats 0.5 ± 0.1%.

Example 4. Bioconversion of flattened grains is carried out similarly to the process described in example 3, but citric acid is used instead of formic acid. Loading: 100 l of purified water, 50 kg of flattened grain, IEC-II, including 30 g of Liquozyme Supra with an activity of 135 EA / g and 2.0 g of Accellerase 1000 with an activity of 2932 EA / g and 25.0 g of Dextrozyme DX with an activity of 170 EA / g

A feed hydrolyzate is obtained having the following composition: humidity 68.0 ± 2.0%, total sugars 23 ± 1.0%, protein 3.8 ± 0.2%, fats 0.5 ± 0.1%.

Analogs of the invention

1. RF patent 2285725.

2. RF patent 2265364.

Claims (1)

A method of producing feed hydrolysates from wheat grains, comprising preparing an aqueous medium with a given pH value, preparing a water-grain suspension, disintegrating - disagglomerating protein-starch agglomerates, heating the reaction mixture during cavitation, gelatinization, enzymatic liquefaction and hydrolysis of starch, that to prepare the water-grain mixture, pre-rolled grain is used, the aqueous medium is prepared by introducing the calculated amount of lemon or ant acid to a pH of 4.0-4.5 units and mixed in a cavitation apparatus for 3-5 minutes at a temperature of 18-40 ° C, heating, disintegration of protein-starch agglomerates, gelatinization is carried out simultaneously for 15-20 minutes at a temperature 60-90 ° C, after which the liquefaction of the gelatinized mass is carried out with a multi-enzyme composition I, including amylosubtiline in an amount of 0.3-0.5 units / g of starch and cellolux in an amount of 0.5-1.0 units / g of cellulose at a temperature 60-85 ° C for 5-10 min or a multi-enzyme composition II consisting of 30 g of Liquozyme Supra with an active 135 EA / g and 2.0 g Accellerase 1000 with an activity of 2932 EA / g, and for saccharification of grain starch, the glucalux enzyme preparation is used in an amount of 2-3 units / g of starch or 25 g of the enzyme preparation Dextrozyme DX with activity of 170 EA / g, which is carried out at a temperature of 60-70 ° C for 2-3 hours to obtain the finished product.