RU2431203C1 - Autoplasty procedure in experiment - Google Patents

Abstract

FIELD: medicine.

SUBSTANCE: wound is irrigated with a metabolite produced by growth of Bacillus subtilis 804 strain bacteria in an amount 1 ml in 1 cm². A transplant is placed.

EFFECT: method provides enhanced transplant adhesion, reduced risk of lysis.

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Description

The invention relates to medicine, to autodermoplasty methods.

Autodermoplasty is used to treat extensive wound defects in surgery. Before the last wound must be prepared. There are conservative and surgical methods for preparing wounds. Conservative methods for preparing granulating wounds in dermoplasty include the use of dressings with antiseptics, antibacterial ointments on a water-soluble basis; dressings containing collagen [1], alginates and hyaluronic acid [2]. These methods involve exposure to still unformed granulations within a few days before skin grafting. Surgical treatment of granulating burn wounds is an excision of granulations followed by simultaneous autodermoplasty [3, 4]. This is a rather traumatic operation, which may be accompanied by profuse blood loss. Criteria for the preparedness of the wound for closure by this method are the presence of clean granules without necrosis and fibrin, bright pink, fine-grained granulations, and the absence of purulent discharge [5, 6]. Despite the variety of methods for preparing wounds, dermoplasty is often accompanied by subsequent lysis of grafts, the frequency of which reaches, according to various authors, 20% [7, 8].

There is no prototype, namely, a method of simultaneous drug exposure to a wound directly during dermoplasty, which significantly reduces the frequency of transplant lysis.

The novelty of the study is that the bacteria of the strain Bacillus subtilis 804 produce human fibroblast growth factor.

A significant difference of the proposed method is that immediately before laying the graft, the wound is irrigated with the metabolite formed during the growth of bacteria of the strain Bacillus subtilis 804, in an amount of 1 ml per 1 cm ² .

The metabolite is a colorless liquid that is formed by growing a culture of Bacillus subtilis 804 and then subjected to purification by ultrafiltration. The strain Bacillus subtilis 804 is deposited in the collection of microorganisms GISK them. L.A. Tarasevich. The metabolite contains human fibroblast growth factor. This fact is confirmed in the Scientific and Production Center for Medical Biotechnology (act No. 1-13 / 10 of 03.22.89). The inventors first discovered that the use of this substance improves the engraftment of a skin graft.

The following experimental studies were conducted. All animal experiments were performed in accordance with the requirements of the "European Convention for the Protection of Vertebrate Animals Used for Experimental and Other Scientific Purposes" (Strasbourg, 1985), under the supervision of the Ethics Committee of the Orenburg State Medical Academy.

Example 1

A male rat weighing 180 g, of the August line, under fluorotan anesthesia, was deeply burned with an area of 2% on a previously shaved area of the skin of the back. 7 days after the formation of the scab, a necrectomy was performed. On the third day after necrectomy, a granulating wound formed. Autodermoplasty was performed. The graft was taken from the shaved back area in a vintage way. Immediately prior to graft placement, the wound was irrigated with 1 ml of the bacterial metabolite Bacillus subtilis 804. The graft adhesion occurred in the second minute. The rat was observed for 21 days. The wounds healed without complications 10 days after autodermoplasty.

Example 2

A male rat weighing 190 g, of the August line, under fluorotan anesthesia, a deep contact burn of 2% was applied to a previously shaved area of the skin of the back. 7 days after the formation of the scab, a necrectomy was performed. On the third day after necrectomy, a granulating wound formed and autodermoplasty was performed. The graft was taken from the shaved back area in a vintage way. Immediately prior to graft placement, the wound was irrigated with 0.9% sodium chloride in an amount of 1 ml. Graft adhesion occurred after 1 hour 50 minutes. The rat was observed for 21 days. Two days after autodermoplasty, graft lysis occurred.

Forty rats under fluorotanic anesthesia suffered deep contact burns with an area of 2% in the back. After the formation of the scab, necrectomy was performed, and after cleansing the wounds, autodermoplasty was performed. Twenty rats were treated using the test drug locally with autodermoplasty at a dose of 1 ml once, in twenty rats the graft bed was irrigated with saline in an amount of 1 ml (control group). In rats treated with a metabolite of bacteria of the strain Bacillus subtilis 804, graft adhesion occurred on average in the third minute. In animals to which physiological saline was applied, adhesion occurred after 2 hours. After 10 days, all animals treated with the experimental preparation in a dose of 1 ml, the wounds healed without complications. In the control group, lysis was observed in five animals (25%) (Table 1).

Table 1 Group of animals Lysis Healing Abs. (%) Abs. (%) Experience (received metabolite) - 20 (100%) Control (received saline solution) 5 (25%) 15 (75%) p> 0.001 p> 0.001

The positive effect of the proposal lies in the fact that when the bacteria metabolite Bacillus subtilis 804 is used to treat a wound of a bacterial metabolite, the adhesion of the transplant is significantly accelerated and the frequency of its lysis is reduced. The method can be used in conjunction with surgical preparation of the wound.

Information sources

1. Dobysh S.V., Vasiliev A.V., Shurupova O.V. Modern dressings for the treatment of wounds in the second phase of the wound process. // Materials of the International Conference, ed. V.D. Fedorov, A.A. Adamyan. - M .: 2001. - P.115.

2. Della Valle F., Rastrelli A., Calderini G., Romeo A. Preparation of highly hydrated self-supporting hyaluronic acid containing films. Patent US N 5618561; 1997.

3. Carvajal H.F., Parks D.H. Burns in children: Translation from English. - M.: Medicine, 1990 .-- 510 p.

4. Alekseev A.A., Kudzoev O.A., Tituma P.N., Klimento M.V. et al. Surgical treatment of granulated wounds in burned. // Materials of the International Congress "Combustiology at the Turn of the Century". - M .: 2000. - S.131-132.

5. Kolesnikov I.S., Vikhriev B.S. Surgical treatment of deep thermal lesions. - M.: 1962. - 177 p.

6. Petrov V.I. Free skin grafting. - L .: Medicine, Len. Dep., 1964 .-- 145 s.

7. Collection of scientific papers of the I Congress of Combustiologists of Russia. - M.: 2005.

8. Bobrovnikov A.E. Antibiotic prophylaxis of postoperative infectious complications in combustiology: Dis. Cand. honey. sciences. - M .: 2000. - 260 s.

Claims (1)

A method of performing autodermoplasty in an experiment, characterized in that before laying the graft, the wound is irrigated with a metabolite formed during the growth of bacteria of the strain Bacillus subtilis 804, in an amount of 1 ml per 1 cm ² .