RU2396074C1 - Complex preparation for treating acute gastrointestinal disturbances in calves - Google Patents

Abstract

FIELD: medicine.

SUBSTANCE: invention refers to veterinary science, particularly to veterinary protozoology and can be used for treating gastrointestinal disturbances in newborn calves. A complex preparation for treating associative course of cryptosporidiosis, colibacteriosis, salmonellosis, rota- and coronaviral enteritis in calves, characterised by the fact that it represents a bolus consisting of agar pectin 6.0, metronidazole 1.5 and aethonium 0.5 grams introduced in a calf orally in the morning and in the evening for 3 days.

EFFECT: invention provides higher clinical effectiveness.

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Description

The invention relates to veterinary medicine, in particular to the use of a complex preparation in the fight against acute gastrointestinal diseases of newborn calves aged 2-30 days (cryptosporidiosis, colibacteriosis, salmonellosis, rota- and coronavirus infections), which in association may cause clinical similar the course of the disease with diarrheal syndrome.

These diseases in calves are accompanied by a depressed state, diarrhea, refusal to take colostrum, milk, progressive emaciation, anemia and general weakness, and body temperature is elevated.

A known method of using the combined preparation for the treatment of acute gastrointestinal diseases of calves with signs of diarrhea (see patent for invention No. 2226390 C2. Urguyev K.R. and others).

The disadvantages of this method is that polymyxin M, chloramphenicol, furazalidone, which are part of the preparation, with frequent use lead to the selection of drug-resistant microflora, their accumulation in human food products, animal intestinal dysbiosis and environmental pollution.

There is also a method of treating acute intestinal diarrheal infections in humans by ingestion of a 3-5% aqueous pectin solution of 25 ml 4 times a day (see ed. St. No. 1790412. Shavakhabov Sh. Sh. Et al.).

The aim of this invention is to develop a method of preparing a comprehensive preparation for the treatment of gastrointestinal diseases of calves.

It includes agar E 406, consisting of 2 parts: 70% non-sulfonated agarose-poly- (β-D-galactose- (1,4) -3,6-anhydro-α-L galactose), partially methylated at position 6 , and about 30% agaropectin of a similar structure containing galacturonic acid, methyl groups, residues of pyruvic acid and 5-10% sulfate ester. We were interested in the second component (as a means for preparing boluses and a promising herbal preparation against salmonellosis and colibacteriosis). This also includes a drug against cryptosporidiosis - metronidazole powder, manufactured by the industry in accordance with TU 932-016-18450726-98, and etonium, being a bis-quaternary ammonium compound, has antibacterial and antiviral effects.

For the manufacture of boluses from these preparations, a plastic mold with holes with a diameter of 2.5-3.0 cm from under the chocolates was used. First, the required amount of agaropectin was added to them, which was poured with boiling water until a thick slurry was obtained, then metronidazole and etonium were added. The use of such water in the preparation of boluses made it possible to reliably inactivate from the ingress of foreign microflora and achieve better solubility of the drugs used.

Identification of virus associations was carried out by studying fecal samples taken from intestinal contents. In this case, the identification of rotavirus was carried out by diffuse precipitation and ELISA, coronavirus was detected in the hemagglutination reaction with red blood cells of white mice.

For bacteriological studies, crops were made in Petri dishes on nutrient broth, nutrient agar, bismuth sulphite agar and Endo agar. Isolated cultures of Salmonella, Escherichia were subjected to further serological studies up to the determination of serovars, using a set of polyvalent and monoreceptor O-serums.

In total, 150 sick calves with diarrheal syndrome were treated in dysfunctional farms, of which 2 groups were formed (experimental - 100 and control - 50 goals).

Each sick calf of the experimental group was given orally with milk or boiled water in the morning and evening a complex preparation in the form of a bolus containing agaropectin 6.0, metronidazole - 1.5 and ethonia - 0.5 g for 3 consecutive days.

The calves of the control group used polymyxin M at a dose of 20-25 thousand units in combination with furazolidone - 6-10 mg / kg 2 times a day for 6-7 days in a row.

When providing them with preventive medical care in both cases, it was assumed that the average live weight of one head of a given age was 40 kg.

The results of the experiments showed that on the second day after the use of the complex preparation, the general condition of the calves of the experimental group improved markedly, diarrhea stopped, and no pathogenic agents were isolated from the seed. The number of cryptosporidium oocysts in feces samples decreased from 1200 to 316. On the third day, their number did not exceed 3-5 specimens in 20 fields of view of the microscope.

All animals of the experimental group recovered; no phenomena indicating the side effect of the drugs used were noted.

Among the calves of the control group, 5 goals fell ill and fell.

Those animals were considered to have recovered, in which, by the 3rd day (experimental group) or the 6th day (control group), the clinical signs of the disease completely disappeared from the beginning of the use of the drugs (see table).

Thus, in comparison with the analogue, the present invention allows to save the number of calves by more than 2.5-5.0%; exclude the use of antibiotics (chloramphenicol, polymyxin M) and nitrofuran drugs (furazolidone), prevent environmental pollution.

Table The therapeutic efficacy of a complex preparation for the treatment of gastrointestinal diseases of calves (cryptosporidiosis, colibacteriosis, salmonellosis, rota- and coronavirus infections) Indicators Groups experienced control The number of animals in the group one hundred fifty Average live weight, kg 40 40 Single dose of the drug: agaropectin, g / goal 6 - metronidazole, g / goal 1,5 - ethonia, g / goal 0.5 - polymyxin M, thousand units - 20-25 furazolidone, mg / kg - 6-10 The frequency of giving per day, times 2 2 Duration of application, days 3 6 Fell animals, goal - 5 Animals recovered, goal one hundred 45 Therapeutic efficacy,% one hundred 90

List of references

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2. Zarosa V.G. Colibacteriosis of newborn calves / V. G. Zarosa, G. A. Burov, V. G. Burov // J. “Veterinary of farm animals”. M. 2008. - No. 4, - S.10-17.

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6. Potievsky E.G., Shavakhabov S.Sh., Bondarenko V.M., Ashubaeva Z.D. Experimental and clinical study of the effect of pectin on the causative agents of acute intestinal infections / E.G. Potievsky, S.Sh. Shavakhabov, V.M. Bondarenko, Z.D. Ashubaeva // J. Microbiology, Epidemiology and Immunology. Appendix - August - September. M., 1994 .-- pp. 106-109.

7. Chervyakov D.K., Evdokimov P.D., Vishker A.S. / Medicines in veterinary medicine // D.K. Chervyakov, P.D. Evdokimov, A.S. Vishker - M .: Kolos. 1977. P.441.

8. Shavakhabov Sh.Sh., Potievsky E.G., Rashidova R.A. et al. / Method for the treatment of acute intestinal diarrheal infections // A.S. No. 1790412. Bull. - Number 3. 1993.

9. Urguyev K.R., Nuratinov R.A., Nazhalov M.I. / Combined preparation for the treatment of acute gastrointestinal diseases of calves occurring with signs of diarrhea // Patent for the invention No. 2226390 C 2. Bull. - 10, dated April 10, 2004.

Claims (1)

A complex drug for the treatment of the associative course of cryptosporidiosis, colibacteriosis, salmonellosis, rota- and coronavirus enteritis of calves, characterized in that it is a bolus consisting of, g: agaropectin 6.0, metronidazole 1.5 and ethonium 0.5, which are administered the calf orally in the morning and evening for 3 days.