RU2391967C1 - Method of radiation sickness treatment in utility dogs and method of radiation sickness preventing in utility dogs - Google Patents

Abstract

FIELD: medicine.

SUBSTANCE: batch of inventions belongs to the field of veterinary radiobiology, notably to method of radiation sickness treatment and prevention in utility dogs. Treatment method consists of intrapulmonary injection of 1% collargol solution, diluted with 0.5% officinal solution of novocain in amount of 1-3 mg/kg 2 hours after radiation exposure, and of hypodermal injections of 0.5% collargol solution, diluted with 0.5% officinal solution of novocain in amount of 3-5 mg/kg of body mass 15, 24 and 48 hours after radiation exposure. On this background, from 1^st up to 25^th day dogs receive injections of ascorbic acid and 1 ml of 1% vicasol solution and ingest Phytochides - 02 and Mumiyo 5 tablets per dog each as general tonic treatment. Starting from the 5^th day after radiation exposure dogs receive 3 ml of 3% Baytril solution additionally. Prevention method consists of intrapulmonary injections of 1% collargol solution, diluted with 0.5% officinal solution of novocain in amount of 3-5 mg/kg on 5^th, 3^th and 1^st day before radiation exposure; as general tonic treatment, dogs receive injections of ascorbic acid and 1 ml of 1% vicasol solution and ingest Phytochides - 02 and Mumiyo 5 tablets per dog each. On this background, starting from the 5^th day after radiation exposure dogs receive 3 ml of 3% Baytril solution additionally.

EFFECT: methods are highly effective.

3 cl, 3 tbl

Description

The present invention relates to the field of veterinary radiobiology, in particular to a method for the treatment and prevention of radiation sickness of dogs.

Ionizing radiation is a stream of gamma rays and neutrons. The effect of ionizing radiation on the human and animal organism causes radiation sickness, characterized by profound functional and morphological changes in all its systems and organs.

A known method for the treatment and prevention of radiation sickness in humans using intramuscular injection of a 0.5% solution of deoxinate in a volume of 15 ml and prodigiosan intramuscularly in the form of a 0.005% solution of 50-100 μg per person (1).

Known anti-inflammatory and bactericidal agent that strengthens the immune system - collargol. Collargol is used externally in the form of solutions (1-2%) or ointments for the treatment of infected wounds, orally and intravenously for septicemia and pyemia (2).

There is also a method for the treatment and prevention of acute radiation sickness in white rats and rabbits using a 0.5% solution of collargol administered subcutaneously at a dose of 3 mg / kg body weight (3). (Prototype)

The objective of our research was to develop an effective method for the treatment and prevention of radiation sickness in service dogs.

This was achieved due to the fact that collargol with fortifying agents was used to treat and prevent acute radiation sickness in dogs.

The proposed method for the treatment of acute radiation sickness of dogs consists in the following: 2, 15, 24 and 48 hours after irradiation (LD _{90-95 / 45} ), dogs are administered collargol in a 0.5% official solution of novocaine - after 2 hours intrapulmonary (after intercostal space - 4-5 intercostal spaces from the last rib along the shoulder-shoulder joint) in the form of a 1% solution at a dose of 1-3 mg / kg, after 15, 24 and 48 hours - subcutaneously in the form of a 0.5% solution in dose of 3-5 mg / kg body weight.

In addition to collargol, vitamins, antibiotics and nutritional supplements are administered to irradiated dogs. The timing of the introduction of these drugs and their doses are given in table 1.

Specific examples of the therapeutic efficacy of the proposed method are given in table.2. As can be seen from the table, the effectiveness of the method substantially depends on both the dose and the method of application of collargol. The optimal combination is the dose of collargol 2 and 3 mg / kg body weight, respectively, with intrapulmonary and subcutaneous administration. With this combination, 100% survival of dogs in the experimental group was noted (out of 5 dogs, 5 survived) with the death of all dogs in the control group (2 out of 2 dogs).

The proposed method for the prevention of acute radiation sickness of dogs is as follows: 1, 3 and 5 days before exposure to dogs (at a dose of LD _{90-95 / 45} ) intramuscularly (through the intercostal space - 4-5 intercostal spaces from the last rib along the shoulder-joint) into the diaphragmatic lobe using a syringe with a needle, a 1% solution of collargol in a 0.5% official solution of novocaine at a dose of 3-5 mg / kg.

In addition to collargol, vitamins, antibiotics and nutritional supplements are administered to irradiated dogs similarly to the treatment method (Table 1).

Table 1 Auxiliary means of the proposed method for the treatment and prevention of radiation sickness of dogs based on collargol Drug name Dose Application time after irradiation Duration of use Multiplicity of application Nutritional supplements: Phytochitodes 5 tab. After 1 day 25 days Daily Mummy 5 tab. After 1 day 25 days Daily Vitamins: Vikasol (1% solution) 1 ml After 1 day 25 days Daily Ascorbic acid (1% solution) 1 ml After 1 day 25 days Daily Antibiotics: Baytril 3 ml After 5 days 25 days Daily (5% solution) Streptomycin 1 gr With an increase in body temperature in dogs to 39.7 ° C and above 25 days Daily (three times a day) Benzylpenicillin sodium salt 1,000,000 units With an increase in body temperature in dogs to 39.7 ° C and above 25 days Daily (three times a day) Note. 1. During treatment, collargol is administered intramuscularly in the form of a 1% solution at a dose of 2 mg / kg 2 hours after irradiation, then 12-15; 48 and 72 hours subcutaneously at a dose of 3 mg / kg. 2. With prophylaxis, collargol is administered intramuscularly in the form of a 1% solution at a dose of 5 mg / kg for 1-3 days after irradiation.

table 2 The effectiveness of the therapeutic effect of the proposed method Collargol dose, mg / kg Number of dogs Dog groups Observed effect Experience The control Experienced Control Survived Fell Survived Fell in / l PC one - 5 3 2 2 one 0 2 - 2 5 3 2 one 2 0 2 2 3 5 3 2 3 0 0 2 3 5 5 3 2 3 0 0 2 Note: in / l - intrapulmonary 2 hours after irradiation; s / c - subcutaneously 24 and 48 hours after irradiation.

Specific examples of the prophylactic effectiveness of the proposed method are given in table.3. As can be seen from the table, prophylactic efficacy depended on the time of administration of collargol prior to dog irradiation and its dose. So, with the introduction of collargol at a dose of 3-5 mg / kg body weight 1 day before irradiation, 100% survived, while with the same dose for 3, 5 days, 67 and 40% of the dogs, respectively, with 70-100% their death in control.

A decrease in the dose of collargol 1.5 times reduced the effectiveness of its protective effect. So, with the introduction of collargol at a dose of 2 and 3 mg / kg body weight a day before irradiation, 67% (or 2 of 3) and 100% (3 of 3) dogs of the experimental groups survived with 67-100% death in the control, respectively.

Used domestic preparations are well tolerated by dogs. The processing of one dog is in the range of 200-300 rubles.

The proposed methods were tested with positive results in commission tests in conjunction with the specialists of 470 Methodological and Cynological Center for Service Dog Breeding of the Armed Forces of the Russian Federation in 2007.

The proposed methods of treatment and prevention will find wide application in official dog breeding during a special period (military conflict with the use of nuclear weapons or the use of radioactive substances for sabotage purposes).

This will help to keep the number of service dogs, especially of such valuable as mine search dogs. Preserving mine-search dogs in the context of nuclear terrorism will save hundreds of lives of personnel involved in the aftermath of terrorist acts using radioactive substances.

Table 3 The effectiveness of the preventive action of the proposed method The time of administration of collargol before irradiation, days Dose mg / kg Number of dogs Groups Observed effect Experience The control Experienced Control Survived Fell Survived Fell one 2 5 3 2 2 (67%) 1 (33%) 0 2 (100%) 3 5 3 2 3 (100%) 0 0 2 (100%) 5 5 3 2 3 (100%) 0 0 2 (100%) 3 5 5 3 2 3 (100%) 0 0 2 (100%) 8 6 2 4 (67%) 2 (33%) 0 2 (100%) 5 5 8 5 3 2 (40%) 3 (60%) 1 (33%) 2 (67%) Total 36 23 13 17 (70%) 6 (30%) 1 (33%) 12 (67%)

Information sources

1. Prince Vasin M.V. Means of prevention and treatment of radiation injuries. M., 2004 .-- S.149-151.

2. Uch. Mozgov I.E. Pharmacology. M., 1969 .-- 323 p.

3. Bulletin of radiation medicine, No. 3, 1968. - P.63-68.

Claims (3)

1. A method of treating radiation sickness of service dogs, comprising introducing collargol and restorative agents into the body, characterized in that 2 hours after irradiation, dogs are injected with collargol in a 0.5% official solution of novocaine intrapulmonary in the form of a 1% solution in a dose 1-3 mg / kg, and after 15, 24 and 48 hours after irradiation, collargol in a 0.5% official solution of novocaine is administered subcutaneously in the form of a 0.5% solution at a dose of 3-5 mg / kg body weight, at the same time, from the 1st to the 25th day, the dogs are also daily injected with ascorbic acid as general strengthening agents slot and 1% vicasol solution 1 ml each, phytochitodes-02 and mummy 5 tablets per dog, and from the 5th day after irradiation, dogs are additionally given a 3% solution of baytril in a dose of 3 ml. 2. The method of treating radiation sickness of service dogs according to claim 1, characterized in that, with an increase in body temperature, dogs are additionally injected with streptomycin and benzylpenicillin sodium salt of 1 g and 1,000,000 units, respectively, per dog, 3 times a day, every 7-8 hours 3. A method for the prevention of radiation sickness of service dogs, including the introduction of collargol and general restorative agents into the body, characterized in that 1, 3 and 5 days prior to irradiation, dogs are administered intragulmonary collargol in 0.5% official solution of novocaine in the form of 1% - solution in a dose of 3-5 mg / kg body weight, and as general restorative agents, dogs are injected with ascorbic acid and 1% Vikasol solution 1 ml each, phytochitodez-02 and mummy 5 tablets per dog, with 5 days after exposure, dogs are additionally injected with a 3% solution of baytril at a dose of 3 ml.