RU2372944C2 - Wound healing coating - Google Patents

Abstract

FIELD: medicine.

SUBSTANCE: invention concerns medicine, namely surgery and aims at pathogenetically grounded healing techniques for various wounds in the first stage of wound process. The invention represents a wound healing coating characterised by that it contains a hydrophilic fabric base, a hydrogel layer containing acrylic acid and acrylamide with a coupling agent with the hydrogel layer having pH 7.0-7.5 and absorbability 36-44 g/g, a water-soluble biodegradable polymer containing mixed gelatine and poly-N-vinylpyrrolidone, and as biologically active components: fullerene C₆₀, antimicrobic, necrolytic, antifermental and haemostatic agents in certain component relation in the coating, wt %.

EFFECT: invention provides extended range of hydrogel-coated bandaging materials possessing ability to accelerate wound healing and simultaneously to reduce number of complications in treatment, particularly to prevent development of pyodestructive processes.

3 cl, 3 tbl, 7 dwg

Description

The invention relates to medicine, namely to surgery, is intended for pathogenetically substantiated treatment of wounds of various etiologies in the first stage of the wound process. Hydrogel bioactive coating for the treatment of wounds can be used in the treatment of uninfected and infected wounds, including purulent and non-healing, granulating wounds after deep thermal, chemical and radiation burns, in the complex treatment of trophic ulcers and pressure sores in inpatient, outpatient and field conditions .

It is known that sorption of wound discharge, tissue and microbial decay products, neutralization of toxic substances (free radicals, products of lipid peroxidation, etc.) is one of the main tasks of treating wounds in the first phase of the wound process. To implement a multicomponent pathogenetic effect on the wound in surgery, biologically active drainage sorbents with immobilized drugs that provide chemotherapeutic cleansing of the wound are used.

Various drainage sorbents based on polyvinyl alcohol crosslinked with glutaraldehyde are known. For example, “Colladiosorb” with antimicrobial and proteolytic action, “Anilodiovin” with antimicrobial and analgesic effect are such sorbents. Their disadvantages include the absence of antioxidant preparations in their composition, as well as the complications they cause, which are mainly associated with fragmentation of the sorbent in the wound and difficulties in completely removing swollen sorbent granules from the wound, which leads to a relapse of the purulent process [Goryunov S. V ., Romashov D.V., Butivshchenko I.A. Purulent Surgery: Atlas. - M .: Medicine, 2004. - S. 504-510].

Known RF patent No. 2004137811, where a bandage with an adhesive composition is protected for fixing on the skin a two-phase system consisting of hydrophobic and hydrophilic layers. The system is complex enough to be applicable in surgical practice.

It is known that in surgical practice, the most promising means of treating wounds of various etiologies, burns, trophic ulcers, bedsores are hydrogel dressings, which, thanks to the properties of the hydrogel, provide a plasticizing effect on wound tissues, soften necrotic formations due to tissue rehydration, facilitate their mechanical removal and prevent development infections on the surface of the wound and under the scab. Hydrogel dressings create a moist environment in the wound that is optimal for the normal course of regeneration processes. Hydrogels contribute to the elimination of wound and microflora. Dressings fit well and are modeled on wounds with complex relief. They are atraumatic, painlessly removed.

Known gelling dressing material "Activitex-HL" with a sorption capacity of up to 4.5 g / g, which prevents its use in profusely exudating wounds, as well as burns in the hydration stage [RF Patent No. 2101033].

A known dressing for closing and treating burns, in which the sorption layer is represented by a nonwoven material that provides sorption capacity of up to 10 g / g, which prevents the complete absorption of wound discharge during abundant exudation of wounds [RF Patent No. 2275179].

Known hydrogel wound coverings "APPOLO-PAK-AM", "APPOLO-PAK-AI" and others, created on the basis of a copolymer of acrylamide and acrylic acid with the inclusion of antiseptics miramistin or iodovidone and local anesthetic anilokain [Goryunov S. V., Romashov D .V., Butivshchenko I.A. Purulent Surgery: Atlas. - M .: Medicine, 2004. - S. 504-510] and having a sorption capacity of 2-3 g / g, which is not enough for effective treatment of wounds. Such coatings have antimicrobial, anti-inflammatory and analgesic effects, but do not contain antienzyme and antioxidant agents, which reduces their effectiveness in purulent-destructive processes. When opening the package, a significant part of the gel remains on the inner surface (more than 50%), and is not transferred to the wound with a carrier net, which is a significant constructive disadvantage. These wound coverings have low pH values in the range of 5.5-6.0, which in the conditions of local tissue acidosis also reduces their effectiveness.

Closest to the invention is a hydrogel composition for the treatment of wounds of various etiologies according to the patent of the Russian Federation No. 2157243. It contains acrylamide and / or sodium acrylate. The composition is intended for the rehydration of a necrotic substrate and the maintenance of a moist environment in the wound, it is equipped with bioactive drugs. Its disadvantage is the presence of glycerol and a large amount of water in the hydrogel, which reduces its effectiveness in purulent-destructive processes.

These dressings and compositions are contraindicated for use on profusely exudating wounds. They are not able to prevent secondary destructive phenomena and wound complications associated with the accumulation of aggressive metabolic products in the wound: free radicals, proteolytic enzymes, which cause secondary tissue damage and lead to slower healing of wounds.

The aim of the invention is to expand the range of dressings with a hydrogel coating, and with the ability to accelerate wound healing and at the same time reduce the number of complications in their treatment, in particular to prevent the development of purulent-destructive processes.

The problem was solved using a three-layer hydrogel bioactive coating. In the treatment of wounds, they use an absorbent hydrogel with a neutral pH and an absorption capacity of 40 g / g ± 10%, which is obtained on the basis of acrylic acid and acrylamide, bioactive ingredients of multidirectional action, included in a water-soluble biodegradable polymer deposited on a porous cotton base, and most importantly Fullerene immobilized in its composition, maximally showing its reparative, antioxidant and other properties in experimentally selected amounts. Used tissue base allows you to remove the wound cover from the wound when changing dressings as a single unit.

When creating the invention, the authors used an absorbent based on acrylic acid and acrylamide with a crosslinking agent, ammonium persulfate, as a hydrogel coating. Absorbent - gel was applied on a fabric base.

The hydrogel coating for the treatment of wounds in an animal experiment is non-toxic, does not have a local irritating and skin-resorptive effect, has elasticity, a high degree of modeling on a wound. The hydrogel does not fragment, does not exfoliate from the tissue base, which facilitates wound care.

The hydrogel absorbent was synthesized according to a known method by reacting neutralized acrylic acid (AK) and acrylamide (AA) with N, N'-methylene-bis-acrylamide (MBAA) as a crosslinking agent in the presence of a redox system (OVS) ammonium persulfate ( PSA) - tetramethylethylenediamine (TMED) "[J. Appl. Polym. Sci. 1997. V. 64, No. 7. R.1345-1353]. Pre-purified acrylic acid was dissolved in water at a concentration of 30 wt.%. Its neutralization was carried out with a solution of sodium bicarbonate (NaHCO ₃ ) under the control of a pH meter. A pre-calculated volume of NaHCO ₃ solution was gradually added until the pH of the mixture was in the range of 7.0-7.5. The neutralization reaction is accompanied by the release of a large amount of carbon dioxide, therefore, after its completion, the mixture was kept for 5-10 minutes. According to the method, the calculated amount of AA, MBAA, TMED and water was added to the mixture [J. Appl. Polym. Sci. 1997. V. 64, No. 7. R.1345-1353]. The mixture was thoroughly mixed until all components were completely dissolved, after which a repeated control of the pH value was carried out. Then the mixture was cooled to a temperature of 5-10 ° C in order to prevent spontaneous polymerization. After cooling, the calculated amount of PSA [J. Macromol. Sci. Part.C. 1999. V.39, No. 3. P.507-525]. The mixture was thoroughly mixed and poured into a cuvette, at the bottom of which a fabric base of the required size was placed. As a result, a gelation and setting process of the textile carrier with the hydrogel composition takes place. The resulting hydrogel-tissue system was placed in a thermostat (T = 45-55 ° C) for 2-3 hours to achieve a more complete degree of monomer conversion [J. Macromol. Sci. Part.C. 1999. V.39, No. 3. P.507-525].

The hydrogel layer of the wound cover determines its high sorption characteristics (36-44 g / g), allowing for a long time to sorb the wound contents, preventing its stagnation in the wound cavity and subsequent suppuration. The sorption ability of the hydrogel layer was tested in various media; at the same time, its comparative evaluation was carried out with Appolo hydrogel and Diovin draining sorbent (table 1).

The inventive hydrogel layer is elastic, with a moisture content of 75%. Gel comparison "Appolo" contains 95% moisture, does not have elasticity. Absorbent "Diovin" - powder, moisture content - less than 1%.

Table 1 Sorption capacity of absorbents Absorbable substances Hydrogel layer of wound dressing Gel "Appolo" "Diovin" Distilled water 320 g / g 70 g / g 20 g / g Saline 90 g / g 25 g / g 15 g / g Amino acid solution 40 g / g 12 g / g 10 g / g Gelatinol 30 g / g - 10 g / g Pleural exudate 40 g / g 5 g / g 10 g / g Ascitic fluid in the abdominal form of Ehrlich cancer 45 g / g 10 g / g 15 g / g

To neutralize local tissue acidosis, it is important that the hydrogel contains a solution of sodium bicarbonate, which allows you to maintain a pH in the range of 7.0-7.5.

As the fabric base used knitted material, mainly on the basis of cotton thread (yarn). The cellular structure of the knitted material is characterized by a density of 15-19 loop rows per 1 cm, mesh size 1 × 1 mm, a surface density of 110-130 g / m ² , porosity of 54%.

Preferably, a hydrophilic textile cotton thread (yarn) of low linear density ranging from 15 to 33 tex is used. It is also possible to use viscose or polyester complex filaments of the same linear density, providing the effect of capillarity, or wickness of textile material.

The tissue base performs a frame function and allows you to remove the wound cover from the wound as a single unit.

The fabric base used allows it to be saturated with a hydrogel, and has a high degree of adhesion of the hydrogel structure to it.

For a fabric base, a low-extensible knitted fabric has uniform porosity (the number of holes or cells per unit area), hydrophilicity, and low material consumption (surface density). When cutting, the canvas does not crumble and has a stable structure, its edges do not twist, do not open.

It is possible to obtain a fabric of a given width, which is necessary in the manufacture of a textile carrier in the form of a knitted tape, with a width in the range from 0.1 to 0.5 m.

In addition to the hydrogel layer and the tissue base, the third layer is used to achieve a high therapeutic effect, consisting of a biodegradable water-soluble polymer, which includes biologically active directed components and is compatible with this polymer in solubility.

As a bioactive component, the hydrogel coating for the treatment of wounds may contain substances with antioxidant, antimicrobial, antienzyme, hemostatic and necrolytic activity.

As a water-soluble polymer, the hydrogel coating for the treatment of wounds contains gelatin, or collagen, or polyvinyl alcohol.

Fullerene C _{60 was} used as an antioxidant. Its amount in the composition is insignificant, only 0.1-1.0% (wt.%), Calculated on a water-soluble polymer layer. Fullerene in a composition with other biologically active preparations (together) in a hydrogel coating has not been previously used. Its structural features do not allow predicting biological activity in such a composition in advance.

Previously, fullerene C ₆₀ was used to treat wounds as part of an ointment prepared on petrolatum [Krylova L.A. Reparative properties of fullerene C ₆₀ : dissertation abstract for the candidate ... Ph.D. sciences. - St. Petersburg, 2003. - P.23]. The conclusions say that fullerene C ₆₀ accelerates wound healing processes only when illuminated with 200 lux, and in an unlit room (5 lux) it almost does not work. The effect that was obtained using fullerene C ₆₀ in the experiment according to the invention was unexpected, because under an aseptic dressing, the light did not reach the water-soluble polymer layer containing fullerene C ₆₀ . The results obtained according to the claimed invention were unpredictable.

Fullerene is one of the four allotropic modifications of carbon, which has a pronounced antioxidant, immunotropic, membrane-active activity, as well as the ability to stimulate the differentiation of fibroblasts [Piotrovsky LB, Kiselev OI Fullerenes in biology. // St. Petersburg: Rostok Publishing House LLC, 2006. - 336 p .; Bioorg. Med. Chem. - 1996 .-- Vol. 4. - P.767-779].

In the coating proposed by the authors, fullerene accelerates wound healing processes in both the first and second stages of the wound healing process. In the first stage of the wound healing process, due to the biological activity of fullerene, free radical processes that can cause secondary tissue necrosis and, as a result, re-induction of inflammation are blocked. In the second stage of the wound process, fullerene, stimulating the differentiation of fibroblasts, accelerates the maturation of granulation connective tissue and epithelization.

The quantitative content of fullerene in the composition of the wound cover was calculated experimentally on the basis of data obtained in experiments on animals. A decrease or increase in the content of fullerene leads to a decrease in the therapeutic effect.

Fullerene is introduced into the polymer layer in the form of a complex with poly-N-vinylpyrrolidone (PVP), and the C ₆₀ fullerene complex contains from 0.1 to 3.0%.

In addition to fullerene, in a total amount of 8.0-20.0% (wt.%), Therapeutic action ingredients are introduced into the water-soluble polymer layer of the hydrogel coating.

As an antimicrobial component, the inventive hydrogel coating contains dioxidine, or chlorhexidine, or iodopyrone, which helps to prevent infection of "clean" wounds, and also allows the use of a hydrogel coating to treat wounds when they are infected, with the development of purulent-destructive processes caused by both gram-negative and and gram-positive flora. In a biodegradable water-soluble layer, its content is 1.0-5.0% (wt.%).

As an antiferment and hemostatic component, ε-aminocaproic acid was introduced into the hydrogel coating, which helps to suppress the activity of proteolytic enzymes in the wound that cause secondary damage to the cells, and due to pronounced hemostatic properties, quickly stops capillary bleeding. In a biodegradable water-soluble layer, its content is 2.0-5.0% (wt.%).

As a non-proliferative component, the biodegradable water-soluble layer of the claimed hydrogel coating for treating wounds contains urea. Its content in the biodegradable layer is 5.0-10.0% (wt.%).

The total content of biologically active components, excluding fullerene, ranges from 8.0 to 20.0%, which helps to prevent inflammatory processes in the wound and contributes to their full epithelization.

The manufacture of a water-soluble polymer was carried out by preparing an aqueous 30% gelatin solution in a water bath at a temperature of 75 ° C. After cooling the resulting solution to 40 ° C, fullerene and bioactive components were introduced into it. After complete dissolution of all components, the gelatin mixture was poured onto the free side of the fabric base used to form a three-layer hydrogel-fabric-gelatin coating (Fig. 1: scheme, where 1 is a hydrogel absorbent layer, 2 is a fabric base, 3 is a biodegradable water-soluble polymer with biologically active components). After cooling and curing the gelatin, a plate of the required shape and size was cut out of the coating, which was hermetically packed and sterilized by γ-radiation.

The biologically active ingredients in the composition for the treatment of wounds are present in the biodegradable water-soluble layer in the ratio, wt.%:

fullerene C ₆₀ 0.1-1.0 antimicrobial component 1.0-5.0 antienzyme and hemostatic component 2.0-5.0 necrolytic component 5.0-10.0 biodegradable water soluble polymer and PVP rest

Such a composition provides a therapeutic effect and reduces the time for complete healing of wounds by at least 1.5 times.

The combined effect of fullerene with bioactive components on the main links in the pathogenesis of the wound process provides a comprehensive effect of the hydrogel coating on the course of the process, leads to faster repair processes, to a reduction in the healing time of wounds and a reduction in the number of complications.

Hydrogel coating is used for topical treatment of superficial, infected and purulent wounds, granulating wounds after deep burns. If the area of the wound exceeds the size of the hydrogel bioactive coating, it is possible to use several coatings with fixation by their aseptic dressing. Dressings are recommended every other day, taking into account the state of the wound and the volume of exudation. The hydrogel bioactive coating should be used until the discharge of the wound discharge disappears, the wound is cleansed of fibrinous and purulent-necrotic plaque and the beginning of the marginal epithelization of the wound defect.

The hydrogel coating is easily removed from the wound due to its "neurasthenia" in the regenerating surface of the wound, because the tissue base of the hydrogel coating is separated from the wound by a water-soluble polymer layer and is the skeleton of its entire composition structure.

To test the biocompatibility of the absorbent, the hydrogel layer was used in an experiment on 10 rats. A conditionally aseptic wound model was created, which was covered with a hydrogel absorbent layer. Dressings with repeated hydrogel application were performed every other day. Clinically, inflammatory and degenerative phenomena in the wound in the postoperative period were not noted. The absorbing hydrogel layer actively absorbed the wound discharge. According to histomorphological studies, on day 7, a necrotic layer with a focal hemorrhage was insignificant in volume on the wound surface, granulation tissue formation was observed in places with growths of the newly formed adipose tissue. Granulation tissue was unevenly swollen with a large number of thin-walled vessels, some of which were vertically oriented to the surface of the wound. Thus, the classical picture of the regenerative process was revealed, which allowed us to conclude that the hydrogel layer of the wound cover is biocompatible and bioinert.

The effectiveness of the hydrogel bioactive coating for the treatment of wounds in the local treatment of deep burns was studied in an experiment on 10 rabbits (experiment 1) and 10 rats (experiment 2).

Experience 1. On the back of rabbits, under local anesthesia, two burns of 4 cm ² each were modeled using a special device. As a burning surface, a copper plate 2.0 × 2.0 cm in size with a heating temperature of 180 ° C was used. The burn was applied by contact for 10 seconds. A day later, under local anesthesia, a necrectomy of a burn scab was performed, and wounds were treated with a 3% hydrogen peroxide solution. One wound was covered with a hydrogel bioactive coating (experiment) with a 1.0% fullerene content in it, and the other with a gelatin sponge without the inclusion of C ₆₀ fullerene, but containing biologically active components in concentrations similar to the invention (control). After the cessation of wound discharge, cleansing of the wound from fibrinous and purulent-necrotic plaque and the beginning of marginal epithelization of the wound defect in the experimental group, the application of the gelatin plate was applied with the inclusion of a bioactive component and C ₆₀ fullerene. The effectiveness criteria were the speed (time) of healing and the histomorphological picture of wounds on days 7, 10, 14 and 21 (table 2).

Comparison of the data of a planimetric study showed that under the influence of a hydrogel bioactive wound dressing with fullerene content

From ₆₀ 1.0%, the percentage reduction in the area of the wound defect to the initial in the experimental group on the 7th day was 45.7%, on the 10th day - 65.9%, on the 14th day - 90.4%, and the term for complete wound healing - 18 days In the control series, these indicators were as follows: on the 7th day - 17.4%, on the 10th day - 49.6%, on the 14th day - 72.8%, on the 21st day - 93.2%, the period of complete wound healing was 25 days.

table 2 The effect of fullerene C ₆₀ and hydrogel content in wound dressing on post-traumatic regeneration Coating The dynamics of reducing the area of wounds in% of the original 7 days 10 days 14 days 21 days Control (without inclusion of fullerene C ₆₀ ) 17.4 49.6 72.8 93.2 Hydrogel bioactive coating with fullerene content C ₆₀ 1.0% 45.7 65.9 90,4 -

According to histomorphological studies in the wounds of the experimental series on the 7th day after necrectomy, there were no signs of inflammation, the process of cleansing the wounds was completed, islands of granulation tissue appeared at the bottom of the wounds. By 10 days, granulation tissue covered the entire bottom of the wound, was well vascularized, and the epithelium was determined in the border zone. On day 14, the epithelialization was not yet completed, the epithelium did not completely cover the defect, but only crawled from the edges in the form of an epithelial wedge, in the granulation tissue, the formation of a large-loop capillary network with a predominance of vertical loops, differentiation of fibroblasts, collagen fibrogenesis with the conversion of argyrophilic fibers to fuchsinofilny testified to the mature nature of granulation tissue.

In the control group, an inflammatory reaction was noted on day 7 (a large number of neutrophilic leukocytes and macrophages, severe exudation). The appearance of granulation tissue was observed only on the 10th day, it was immature and with a small number of neutrophilic leukocytes. In the underlying tissues, lymphohistiocytic infiltration persisted around the vessels.

By 21 days in the experimental group, complete healing of the wounds was noted. In the control group, the histomorphological picture of regenerates at this time corresponded to the stage of proliferation and maturation of granulation tissue, the epithelium covered only the edges of the wound.

It was established that under the action of hydrogel bioactive wound cover, an earlier completion of the inflammatory process is observed, a distinct stimulation of the growth of granulation tissue and an increase in the processes of regional epithelization, this is facilitated by the presence of C ₆₀ fullerene and the inventive hydrogel absorbent in the composition.

Polyvinyl alcohol and collagen were tested as a water-soluble polymer layer with biologically active components. In similar hydrogel coatings, they showed similar results.

Experience 2. On the back of rats under ether anesthesia simulated two burns with an area of 1 cm ² each using a special device. As a burning surface used a copper plate with a size of 1.0 × 1.0 cm with a heating temperature of 180 ° C. The burn was applied by contact for 10 seconds. A day later, under ether anesthesia, a necrectomy of a burn scab was performed, and wounds were treated with a 3% hydrogen peroxide solution. One wound was covered with a hydrogel bioactive coating (experiment) with a 0.1% fullerene content in it, and the other with a gelatin sponge without C ₆₀ fullerene, but containing biologically active components in concentrations similar to the invention (control). After the cessation of wound discharge, cleansing of the wound from fibrinous and purulent-necrotic plaque and the beginning of marginal epithelization of the wound defect in the experimental group, the application of the gelatin plate was applied with the inclusion of a bioactive component and C ₆₀ fullerene. The effectiveness criteria were the speed (time) of healing and the histomorphological picture of wounds on days 7, 10, 14, and 20 (table 3).

Comparison of the data of a planimetric study showed that under the influence of a hydrogel bioactive wound dressing with fullerene content

From ₆₀ 0.1%, the percentage reduction in the area of the wound defect to the initial in the experimental group on the 7th day was 65.4%, on the 10th day - 96.5%, and the term for complete wound healing - 12 days. In the control series, these indicators were as follows: on day 7 - 36.3%, on day 10 - 65.1%, on day 14 - 73.8%, the term for complete wound healing was 20 days.

According to histomorphological studies, in the wounds of the experimental series on the 7th day after necrectomy, cleansing of the wounds from non-viable tissues was carried out, mainly by macrophages, the formation of well-vascularized granulation tissue and epithelization in the border zone, where the growth of the epithelial wedge under the scab between the fibrinoid and the newly formed granulation tissue (figure 2 - staining with hematoxylin-eosin, an increase of 32 times). In the control group, the histomorphological picture on day 7 indicated a more pronounced inflammatory reaction, while, like in the experimental group, the wound was cleansed of non-viable tissues, however, the processes of granulation tissue formation and epithelization proceeded much more slowly (Figs. 3, 4 - hematoxylin staining -eosin, an increase of 32 times).

Table 3 The effect of the content of fullerene C ₆₀ and hydrogel absorbent in the wound cover on the dynamics of reducing the area of wounds in% of the original Coating Day 7 10 12 fourteen twenty Hydrogel bioactive wound dressing with fullerene C ₆₀ - 0.1% 65,4 96.5 one hundred - - The control 36.3 65.1 - 73.8 one hundred

On day 10 in the experimental group (Fig. 5 - hematoxylin-eosin staining, 32-fold increase) epithelization was not yet completed, the epithelium did not completely cover the defect, but only crawled from the edges in the form of an epithelial wedge, the formation of a large-loop capillary was observed in the granulation tissue networks with a predominance of vertical loops, differentiation of fibroblasts, collagen fibrogenesis with transformation, in contrast to the control (Fig.6 - hematoxylin-eosin staining, 32-fold increase), argyrophilic fibers to fuchsinophilic, which indicates about the more mature nature of granulation tissue.

On the 14th day in the control group, the histomorphological picture corresponded to the stage of proliferation and maturation of granulation tissue, and the epithelium covered only the edges of the wound (Fig. 7 — hematoxylin-eosin staining, 32-fold increase).

The additional inclusion in the biologically active composition of fullerene C ₆₀ contributes to a high therapeutic effect, as well as the presence of the inventive hydrogel absorbent.

An analysis of the effectiveness of topical application of hydrogel bioactive wound dressing for deep burns showed that reparative processes under these conditions proceed faster throughout the wound process, and not in its individual stages.

It was found that under the action of the inventive hydrogel bioactive wound cover, healing takes place no less than 1.5 times faster (complete healing takes 12-14 days, while in the control it is 20 days, i.e. 8 days longer) . Under the action of a wound dressing containing C ₆₀ among the biologically active ingredients, introduced into their composition in the form of C ₆₀ / PVP, and containing as an absorbent with a pH of 7.0-7.5 a hydrogel based on acrylamide, acrylic acid and a crosslinking agent MBAA , there is an earlier completion of the inflammatory process, a distinct stimulation of the growth of granulation tissue and an increase in the processes of regional epithelization.

Claims (4)

1. The coating for the treatment of wounds, characterized in that it contains a hydrophilic tissue base, a hydrogel layer containing acrylic acid and acrylamide with a crosslinking agent, while the hydrogel layer has a pH of 7.0-7.5 and has an absorption capacity of 36-44 g / g, a water-soluble biodegradable polymer containing a mixture of gelatin with poly-N-vinylpyrrolidone, as well as biologically active components: fullerene C ₆₀ , antimicrobial, necrolytic, antienzyme and hemostatic, with the following components in the coating, wt.%:
hydrophilic fabric base 5.6-6.4 hydrogel layer containing acrylic acid and acrylamide with a crosslinking agent, 46.8-51.5 water soluble biodegradable mixture containing polymer gelatin with poly-N-vinylpyrrolidone, as well as biologically active components: fullerene C ₆₀ , antimicrobial, necrolytic, antienzyme and hemostatic 42.9-46.8 2. The coating for the treatment of wounds according to claim 1, characterized in that the water-soluble biodegradable polymer layer contains biologically active components in the following ratio, wt.%:
fullerene C ₆₀ 0.1-1.0 antimicrobial component 1.0-5.0 antienzyme and hemostatic Components 2.0-5.0 necrolytic component 5.0-10.0 3. The coating for the treatment of wounds according to claim 1, characterized in that it contains dioxidin as an antimicrobial component, ε-aminocaproic acid as an antienzyme and hemostatic component, and urea as a non-lytic component. 4. The coating for the treatment of wounds according to claim 1, characterized in that the tissue base has a porosity of 54% and a surface density of 110-130 g / m ² .

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