RU2370262C1 - Medication for treatment and prevention of diseases, induced with deficit of selenium for agriculture animals - Google Patents

Abstract

FIELD: medicine.

SUBSTANCE: invention relates to veterinary. Medication for treatment and prevention of diseases, induced with deficit of selenium for agriculture animals, includes sodium selenite, levamisole base and ascorbic acid and water for injections, with following component ratio in wt %: sodium selenite 0.05-0.4; levamisole base 3.0-8.0; ascorbic acid 5.0-15.0; water for injections - the remaining part.

EFFECT: invention provides immunoprotecting, anti-stress and antioxidant action of medication and reduction of costs for its production.

8 tbl, 4 ex, 1 dwg

Description

FIELD OF THE INVENTION

The invention relates to veterinary medicine, in particular to drugs for the treatment and prevention of diseases associated with selenium deficiency for farm animals.

State of the art

Known sodium selenite. In animal husbandry, sodium selenite has long been used, which is a white or yellowish free-flowing powder. Sodium selenite is classified as a poisonous potent substance, since a drug at a dose above 1 mg / kg in animals causes signs of acute poisoning, therefore, the drug must be accurately dosed and used with great care (See Kalnitsky B.D. Mineral substances in animal feeding. - M., 1985. - S.12-15, 24-28). In disadvantaged areas and farms, it is recommended that pregnant females and offspring be treated with selenium preparations. Cows from the second half of pregnancy are injected subcutaneously with 30-40 mg of sodium selenite in the form of a 0.1% aqueous solution, the injections are repeated after 30-40 days and stopped 15-20 days before calving (See Frolova N.A., Nazarenko I.I., Suslova R.G. Selenium content in feed of the Non-chernozem zone of the RSFSR // Sb. Scientific tr. MVA, 1980. - PO. - P.55).

The disadvantage of this drug is the low effect of increasing the overall resistance of the body.

A known drug by the method of feeding piglets, providing for the introduction into the diet based on barley, corn, wheat premix containing vitamins A, D, E, B ₁₂ , manganese, cobalt, zinc, iron, copper, iodine, antioxidant, antibacterial drug, filler - extruded corn, at the same time selenium and citric acid are added to the premix, and biovit is used as an antibacterial drug and the obtained premix is fed to piglets at the age of 21-60 days in the amount of 0.9-1.5 from the main diet, and the indicated comp Prex premixes in the following ratio, wt.%:

Vitamin A 640 660 million I.E. Vitamin D 64 66 million I.E. Vitamin E 0.1-0.3 Vitamin B ₁₂ 0.0001-0.0006 Manganese 0.2-0.7 Zinc 1.1-1.4 Iron 0.6-0.8 Copper 1.1-1.4 Selenium 1.1-1.4 Iodine 0.005-0.009 Cobalt 0.001-0.004 Biovit 1.0-1.5 Lemon acid 0.1-0.4 Antioxidant 0.04-0.06 Filler - Extruded Corn  - the rest

(see Pat. RU No. 2083131, class A23K 1/00, A23K 1/16, publ. 07/10/1997)

The disadvantage of this drug is the high cost, insufficient increase in body resistance, inconvenience of dosage.

The closest in technical essence and the achieved positive effect and adopted by the authors for the prototype is a preparation containing 1 ml of 50 mg of tocopherol acetate and 0.5 mg of sodium selenite in the form of a water-soluble complex (See Orobets V.A., Belyaev V.A. , Kireev IV Influence of E-selenium on the reproductive function of cows - Russian Veterinary Journal. - Special issue, May - 2007. - P.18-19).

The disadvantages of this drug are the low effect of increasing the overall resistance of the body and the ability to redox processes.

Disclosure of invention

The objective of the invention is to develop a drug for the treatment and prevention of diseases associated with selenium deficiency for farm animals, with anti-stress effect, immunoprotective effect and lower manufacturing costs.

The technical result, which can be achieved using the present invention, is reduced to the immunoprotective effect, anti-stress and antioxidant effect and lower manufacturing costs.

The technical result is achieved using a drug for the treatment and prevention of diseases associated with selenium deficiency, for farm animals, including sodium selenite and water for injection, while the drug additionally contains levamisole base and ascorbic acid in the following ratio, in wt.%:

Sodium Selenite 0.05-0.4 Levamisole base 3.0-8.0 Ascorbic acid 5.0-15.0 Water for injections - the rest

The essence of obtaining the drug for the treatment and prevention of diseases associated with selenium deficiency for farm animals is as follows: starting materials, in wt.%, Namely: sodium selenite 0.05-0.4, levamisole base 3.0-8 , 0, ascorbic acid 5.0-15.0 and water for injection - the rest is mixed under aseptic conditions and packaged.

The pharmacological effect of the drug is that the biological role of selenium is determined by the presence of selenoproteins in the active centers, such as glutathione peroxidase, thioredoxin reductase, etc. (See Burk RF, Hil KE, Motley AK Selenoprotein metabolism and function: evidence for more that one function for selenoprotein . J. Nutr., 2003, 33: P.1517-1520). Selenium, entering the body in the form of selenite or selenium-containing amino acids, is included in a large number of proteins - selenoproteins. (See Bamsal M.P., Ip C., Medina D. // Proc. Soc. Environ. Biol. Med. - 1991. - 196. - P.147-154.) (See Burk RF // J .Nutr. - 1989. - 119, N 7. - P.1051-1054.) (See Sunde RA OARDC Special Circular. - 1999. - N 167. - P.45-57.) Currently, selenium along with vitamins A, E and C are considered one of the four major components of the non-enzymatic pathway of the antioxidant-antiradical system of body protection. (See Maistrov V.I., Galochkina V.P., Schevelev N.S. Antioxidant-antiradical and thioldisulfide systems of breeding bulls under the influence of a complex of biologically active substances // Agricultural Biology. - 2006, No. 2. - P.64- 68). The biochemical functions of selenium are very similar to those of vitamin E. Selenium and vitamin E act together and have antioxidant ability (See Diplock A.T. Antioxidant nutrition and disease prevention - an overview // American journal of clinical nutrion. - 1991, V.53 . - S.189-193). One selenium atom can replace 700-1000 molecules of vitamin E. The antioxidant activity of selenium-containing proteins is 500 times higher than that of vitamin E (See Evdokimov P.V., Artemiev V.I., Vitamins, trace elements, biostimulants and antibiotics in animal husbandry and veterinary medicine. - L., 1974. - 217 p.).

With selenium deficiency, the proliferative activity of T cells during their stimulation by mitogens, concanavalin A, phytohemagglutinin, etc., sharply decreases. Adult ruminants exhibit greater resistance to the effect of selenium deficiency on lymphocyte proliferation compared to young animals (See Turner RJ, Finch JM Selenium and the immune response. Proceedings of the Nutrition Society, 50: 275-285, 1990). A high positive correlation was found between a decrease in plasma selenium concentration and a parallel decrease in the number of T cells in immunodeficiency syndrome (See Taylor EW Selenium and cellular immunity: evidence that selenoproteins may be encode in the +1 reading frame everlapping the human CD4, CDS and HLA-DR genus // Biological Trace Element Research. - 1995, N49. - P.73-78).

Biological functions of ascorbic acid: participation in redox processes, regulation of carbohydrate metabolism, blood coagulation, stimulation of hematopoiesis, the formation of steroid hormones of the adrenal glands, normalization of capillary permeability (See Melnichenko V.I. Antioxidants in veterinary medicine: new opportunities. - Veterinary clinic. - 2006, No. 7). One of the main properties of the vitamin is the ability to redox transformations. Ascorbic acid is able to oxidize to dehydroascorbic acid, and thus together with it it represents a redox system that loses and attaches electrons and protons. Vitamin C restores vitamin E but oxidizes itself (See Yi OS, Han D., Shin HQ Synergistic antioxidative effects of tocopherol and ascorbic acid in fish oil (lecitin) water system. J. Am. Oil Cyem. Soc. - 1991; 5 (8) .- S.881-883). Vitamin C supplements improve immune responses by maintaining levels of vitamin E in the blood and tissues; vitamin E is also a pronounced stimulant of the immune system (See Bendich A., D'Apolito P., Gabriel E., Machlin IJ Modulation of the immune system function of guinea pigs by dietary vitamin E and C following exposure to oxygen // Fed. Proc. - 1983. - 42. - P. 923).

Vitamin C restores selenite to elemental selenium, while elemental sulfur and selenium are easily reduced, forming sulfides and selenides containing two or more sulfur and selenium atoms. Protein selenocystin can be coupled to elemental selenium via electron transfer to form selenium-containing bonds.

Levamisole (Levamisolum) - 2,3,5,6-tetrahydro-6-phenylimide-zo [2,1-b] thiazole hydrochloride. White amorphous or crystalline powder. Easily soluble in water. This drug was originally proposed as an anthelmintic. When studying the anthelmintic action of levamisole, it was found that it increases the general resistance of the body and can be used as a means for immunotherapy. Experiments on isolated cells and observation of healthy and sick people showed that the drug is able to restore the altered functions of T-lymphocytes and phagocytes and, due to its timomimetic effect, can regulate the cellular mechanisms of the immunological system. More detailed studies have shown that levamisole, selectively stimulating the regulatory function of T-lymphocytes, can act as an immunomodulator that can enhance a weak reaction of cellular immunity, weaken a strong one and not act on a normal reaction. In connection with these properties, levamisole has been proposed for the treatment of various diseases, in the pathogenesis of which they attach importance to immunogenesis disorders: primary and secondary immunodeficiency states, autoimmune diseases, chronic and recurrent infections, tumors, etc. Levamisole as an immunostimulating drug can be effective in the complex therapy of various diseases (See Mashkovsky M.D. - M38 Medicines: In 2 volumes. T.2. - 11th ed. ster. M: Medicine, 1988. - 576 p. ISBN 5 - 225 - 00036- 3).

Levamisole is a highly effective anthelmintic used in gastrointestinal and pulmonary nematodes. The drug causes an increase in the number of both 1 - and B-lymphocytes, the titer of natural antibodies and the process of antibody formation in intact animals. The data obtained showed that levamisole in intact animals mainly stimulates cellular immunity. It causes a quick effect: after a day, the number of T-lymphocytes increases 2 times in comparison with control animals. In spontaneously infected on the same days, levamisole at a dose of 10 mg / kg by ADV caused a 5-fold increase in the level of T-lymphocytes. The increasing activity of T-lymphocytes, which occurs under the influence of levamisole, obviously plays an important role in the ability of the drug to increase the immunobiological properties of the body (See Daugalieva E.Kh. Filippov V.V. Immune status and ways of its correction in helminthiases of farm animals. M., VO Agropromizdat. - 1991. - 188 p.).

Water was chosen as a solvent, since all components of the drug are water-soluble compounds.

The implementation of the invention

Examples of specific performance, obtaining and testing the drug for the treatment and prevention of diseases associated with selenium deficiency for farm animals.

Example 1

The drug for the treatment and prevention of diseases associated with selenium deficiency for farm animals is prepared by mixing the components under aseptic conditions and dissolving in water for injection in the following ratio of components, in wt.%:

Sodium Selenite 0,03 Levamisole base 2.5 Ascorbic acid 4,5 Water for injections - the rest

with subsequent packaging and sterilization.

The obtained preparation, when applied to cows during pregnancy 60 and 30 days before calving, while analyzing the morphological and biochemical parameters of blood, showed relatively low stabilization of hematopoiesis, protein and lipid metabolism, and concentration of γ-globulins in the blood.

Example 2

Carried out analogously to example 1, but take the following ratio of components, in wt.%:

Sodium Selenite 0.05 Levamisole base 3.0 Ascorbic acid 5,0 Water for injections - the rest

The preparation obtained when applied to cows during pregnancy 60 and 30 days before calving, when analyzing the morphological and biochemical blood parameters of experimental animals, showed that the preparation stabilizes hematopoiesis, protein and lipid metabolism, increases the concentration of γ-globulins in the blood by 9.0%, while reducing the content of α-globulins by 7.0%, he activated the body's antioxidant defense system, which was manifested by a decrease in the blood concentration of malondialdehyde in animals of the experimental group by the end of pregnancy by 27%, while as in the cows of the control group, it increased from 1.25 μmol / L to 1.47 μmol / L.

Example 3

Carried out analogously to example 1, but take the following ratio of components, in wt.%:

Sodium Selenite 0.4 Levamisole base 8.0 Ascorbic acid 15.0 Water for injections - the rest

The obtained preparation, when applied to cows during pregnancy 60 and 30 days before calving, while analyzing the morphological and biochemical blood parameters of experimental animals, showed that the preparation stabilizes hematopoiesis, protein and lipid metabolism, and increases the concentration of γ-globulins in the blood by 9.3% while a decrease in the content of α-globulins by 7.1%, it activates the body's antioxidant defense system, which was manifested by a 36% decrease in the blood concentration of malondialdehyde in animals of the experimental group by the end of pregnancy, while to the cows of the control group there was an increase from 1.29 μmol / L to 1.47 μmol / L, i.e. by 12.5%.

Example 4

Carried out analogously to example 1, but take the following ratio of components in wt.%:

Sodium Selenite 0.45 Levamisole base 9.5 Ascorbic acid 18.0 Water for injections - the rest

The preparation obtained when applied to cows during pregnancy 60 and 30 days before calving, when analyzing morphological and biochemical blood parameters, did not show pronounced dynamics towards stabilization of hematopoiesis, lipid and protein metabolism, and also contributed to a decrease in the number of red blood cells by 7.6% compared with the control group and in this regard, an increase in the color index to values above the physiological norm.

Thus, examples 2 and 3 are the most optimal, since as a result of the use of the drug, the concentration of selenium in the blood increased 4.7 times, the activity of catalase and peroxidase in the blood increased to 13 and 18.5%, respectively, and the concentration of malondialdehyde decreased, respectively up to 36% and was significantly lower than in animals of the control group.

For toxicological studies and evaluation of the action of the drug for the treatment and prevention of diseases associated with selenium deficiency for farm animals, shown in tables 1-8 and on the graph (see drawing 1), the following ratio of components was taken, in wt.%:

Sodium Selenite 0.2 Levamisole base 4.0 Ascorbic acid 7.5 Water for injections - the rest

Toxicological studies of the drug were performed for the treatment and prevention of diseases associated with selenium deficiency for farm animals. To determine lethal doses of the drug, clinically healthy white mice weighing 20-22 g were used, the studies were carried out according to the “Guidelines for the toxicological evaluation of new drugs for the treatment and prevention of non-communicable animal diseases” (All-Russian Research Institute of Non-communicable Animal Diseases, 1987). The active substance of the drug is a combination of sodium selenite, levamisole base and ascorbic acid. The intramuscular route was chosen as the route of administration. This medication is intended for intramuscular administration to farm animals. Table 1 presents the research data of the drug for the treatment and prevention of diseases associated with selenium deficiency for farm animals for acute toxicity.

Table 1 Acute toxicity of the drug for the treatment and prevention of diseases associated with selenium deficiency for farm animals with a single intramuscular injection (mg / kg DV) (n = 5) Animal species Toxicity parameters SLD ₅₀ MTD LD ₀ LD ₁₆ LD ₅₀ LD ₅₀ LD ₁₀₀ White mice 11.11 2.92 27.78 34.51 45.63 55.57 ± 0.006

Laboratory white mice were used to evaluate the effect of the drug for the treatment and prevention of diseases associated with selenium deficiency for farm animals on hematological parameters; animals of the experimental groups were injected intramuscularly with the test drug in various doses (see table 2).

table 2 Hematological blood counts of white mice (n = 5) Group Dose, mg / kg DV Red blood cells, mln / μl Color indicator, units Hemoglobin, g / l White blood cells, thousand / μl control - 7.8 ± 0.7 1.14 ± 0.09 150 ± 7 5.3 ± 1.1 1 experienced 1.17 8.9 ± 1.3 1.05 ± 0.04 158 ± 2 8.0 ± 0.8 2 experienced 1,6 7.6 ± 1.1 1.08 ± 0.07 139 ± 11 9.83 ± 1 3 experienced 2.05 6.0 ± 0.5 1.12 ± 0.1 116 ± 19 9.12 ± 1.8

Analyzing the results obtained, it is possible to note the positive effect of the drug for the treatment and prevention of diseases associated with selenium deficiency for farm animals, at a dose of 1.17 mg / kg DV for some hematological parameters: an increase in the number of red blood cells, hemoglobin level, stabilization of the number of leukocytes. This served as a starting point for determining the dose of the drug in assessing the effect of the drug on the body of farm animals.

Table 3 Leukogram (n = 5) Group Dose, mg / kg DV Basophils Eosinophils Neutrophils Lymphocytes Monocytes YU P FROM control - - 1 ± 0.03 - 3 ± 0.05 27 ± 4 66 ± 7 2 ± 1 1 experienced 1.17 - - - 2 ± 0.07 28 ± 3 70 ± 9 4 ± 0.6 2 experienced 1,6 1 ± 4 ± 0.09 1 ± 0.05 4 ± 0.09 25 ± 4 67 ± 4 2 ± 0.3 3 experienced 2.05 - 4 ± 0.05 - 4 ± 0.05 34 ± 9 73 ± 7 2 ± 0.9

Table 3 shows the differential count of leukocytes, which is of great importance in assessing blood formation, the presence of toxic effects on the body and inflammatory processes, and the body's resistance to infections.

To assess the effect on the body and the increase in live weight, clinically healthy rabbits kept in a vivarium with a complete diet were used (see Tables 4-6).

Table 4 Biochemical parameters of blood serum of rabbits after the use of the drug for the treatment and prevention of diseases associated with selenium deficiency for farm animals at a dose of 1.17 mg / kg in terms of DV (n = 5) Group Total protein, g / l Protein fractions,% Cholesterol, mg% Albumin Globulins α β γ Before drug administration Experience 60.1 ± 7.7 55.2 ± 5.5 11.4 ± 2.0 11.4 ± 2.5 22.0 ± 4.7 87.6 ± 9.5 The control 62.7 ± 3.2 57.3 ± 3.2 10.8 ± 0.1 13.2 ± 1.7 18.7 ± 1.46 84.8 ± 7.5 8 days after drug administration Experience 62.1 ± 5.5 57.9 ± 2.7 10.5 ± 1.0 12.3 ± 1.9 19.3 ± 1.1 80 ± 4,5 The control 62.6 ± 2.9 56.5 ± 1.7 10.8 ± 1.5 13.3 ± 1.1 19.3 ± 1.3 84.8 ± 4.3 15 days after drug administration Experience 67.8 ± 4.6 58.4 ± 0.8 10.0 ± 0.7 11.3 ± 1.4 20.2 ± 2.2 74.6 ± 2.7 The control 62.8 ± 2.1 59.6 ± 1.7 11.1 ± 0.8 12.4 ± 1.0 19.6 ± 1.7 87.1 ± 2.3 23 days after drug administration Experience 68.1 ± 4.0 59.3 ± 2.5 10.7 ± 0.7 11.2 ± 0.95 19.3 ± 1.7 73.5 ± 3.6 The control 62.9 ± 1.0 56.9 ± 0.9 11.1 ± 0.2 12.2 ± 0.9 19.7 ± 1.4 86.4 ± 3.2 31 days after drug administration Experience 69.2 ± 4.1 58.2 ± 1.8 9.7 ± 0.9 10.3 ± 0.8 22.7 ± 1.2 74.7 ± 1.7 The control 63.5 ± 2.7 58.2 ± 2.8 11.1 ± 0.8 11.9 ± 0.7 18.7 ± 2.7 86.7 ± 2.9

Table 5 Biochemical and hematological blood parameters of rabbits after the use of the drug for the treatment and prevention of diseases associated with selenium deficiency for farm animals at a dose of 1.17 mg / kg DV (n = 5) Group Glucose, mmol / L Hemoglobin, g / l Color indicator Red blood cells, mln / μl White blood cells, thousand / μl Catalase activity, μm H ₂ O ₂ / l · min · 10 ³ Selenium, mcg% Before drug administration Experience 6.73 ± 1.7 105 ± 8.5 1.1 ± 0.07 4.9 ± 0.8 0.8 7.0 ± 0.9 0.3 ± 0.1 1.16 ± 0.42 The control 6.3 ± 0.8 104.7 ± 6.1 1.1 ± 0.04 4.9 ± 0.3 6.8 ± 0.4 0.32 ± 0.2 1.23 ± 0.7 8 days after drug administration Experience 5.9 ± 0.8 108.5 ± 5.2 1.04 ± 0.04 5.4 ± 0.2 9.0 ± 0.8 0.83 ± 0.17 9.18 ± 0.8 The control 6.0 ± 0.1b 105.2 ± 3.3 1.1 ± 0.07 4.9 ± 0.3 6.7 ± 0.8 0.41 ± 0.13 1.50 ± 1.01 15 days after drug administration Experience 5.31 ± 0.5 111 ± 3.9 1.0 ± 0.04 5.7 ± 0.3 9.1 ± 0.8 0.84 ± 0.08 8.9 ± 0.35 The control 6.0 ± 0.2 104 ± 2,3 1.07 ± 0.07 5.3 ± 0.4 6.6 ± 0.78 0.44 ± 0.06 1.26 ± 1.3 23 days after drug administration Experience 5.25 ± 0.4 111.7 ± 2.9 0.98 ± 0.05 5.9 ± 0.3 8.2 ± 0.8 0.75 ± 0.06 8.53 ± 0.54 The control 6.11 ± 0.34 107.5 ± 3.1 1.05 ± 0.06 5.3 ± 0.4 6.0 ± 0.7 0.35 ± 0.15 1.09 ± 1.14 31 days after drug administration Experience 5.24 ± 0.5 111 ± 2.9 0.95 ± 0.03 6.0 ± 0.3 8.1 ± 0.5 0.74 ± 0.03 8.17 ± 0.27 The control 6.21 ± 0.3 108.7 ± 3.4 1.04 ± 0.04 5.4 ± 0.3 5.9 ± 0.3 0.40 ± 0.03 1.3 ± 1.4

Table 6 The dynamics of the live weight of rabbits after using the drug for the treatment and prevention of diseases associated with selenium deficiency for farm animals at a dose of 1.17 mg / kg in terms of DV (n = 3) Group Before introduction After 8 days In 15 days In 23 days After 31 days Experience 2.36 ± 0.2 2,50 ± 0,2 2.61 ± 0.2 2.69 ± 0.2 2.81 ± 0.2 The control 2.40 ± 0.3 2,50 ± 0,3 2.58 ± 0.3 2.63 ± 0.3 2.73 ± 0.3

An analysis of the results indicates an increase in the total protein content in the blood serum of experimental animals by 13.2%, catalase activity in the blood by 59.5%, and selenium in the blood by 7 times, while in the control group these indicators remained at the same level. Before administration of the drug, an increase in blood glucose was observed in rabbits of both groups, the use of the drug contributed to the normalization of carbohydrate metabolism and a decrease in glucose level by 22.2%, while in the control this indicator remained almost unchanged. The serum cholesterol content during the experiment decreased in the experimental group by 14.7%, while in the control group it increased by 1.6%, and the leukocyte content in the control animals decreased by 13.2%, while in the experimental animals it increased by 13.6 (due to the presence of an immunostimulant in the composition of the drug). The increase in live weight over 31 days in the control group averaged 330 g, and in the experimental 450 g, which is 26.6% more.

To test the drug for the treatment and prevention of diseases associated with selenium deficiency for farm animals, 10 animals were selected on sheep, taking into account the principle of analogues, which were divided into two groups. The animals of the first group were injected intramuscularly with a drug for the treatment and prevention of diseases associated with selenium deficiency for farm animals at a dose of 1.17 mg / kg in DV. Sheep of the second group did not receive the drug and served as a control. The test results are presented in tables 7, 8.

An increase in the total protein content in the blood serum of the experimental sheep and a decrease in the blood glucose level to the physiological norm were found, the vitamin C content increased 1.9 times. Catalase activity in the blood of the experimental group increased by 42.6%, and in the blood of the control group - by 14.5%; peroxidase activity in the experiment and control was 42 and 5.1%, respectively. The use of the drug contributed to a decrease in the concentration of malondialdehyde in the blood by 76%.

Summing up the studies, it is noted that the use of the drug for the treatment and prevention of diseases associated with selenium deficiency for farm animals, helps to activate the antioxidant defense system of the body, which is expressed in an increase in the activity of catalase, peroxidase, and vitamin C, a decrease in the concentration of malondialdehyde in the blood - under-oxidized product of free radical reactions. When analyzing the dynamics of the content of selenium in the blood, we observe an increase in the concentration of the selenium to a physiological norm within a week. When the drug affects the body, there is a noticeable increase in the number of leukocytes associated with the presence of levamisole in its composition - a compound with immunostimulating properties. The use of the drug for the treatment and prevention of diseases associated with selenium deficiency for farm animals was characterized by a decrease in blood cholesterol to physiological norms and normalization of protein and carbohydrate metabolism.

Table 7 Biochemical parameters of sheep blood serum after the use of the drug for the treatment and prevention of diseases associated with selenium deficiency for farm animals at a dose of 1.17 mg / kg in DV (n = 5) Group Total protein, g / l Protein fractions,% Vit.S, mg / l Albumin Globulins α β γ Before drug administration Experience 62.5 ± 3.8 36.2 ± 1.7 16.8 ± 0.3 9.0 ± 0.3 38.0 ± 1.6 2.13 ± 0.23 The control 59.9 ± 1.8 36.8 ± 1.2 17.4 ± 2.6 10.2 ± 1.3 35.5 ± 4.2 2.1 ± 0.1 15 days after drug administration Experience 68.1 ± 4.6 42.06 ± 2.8 15.6 ± 0.9 8.5 ± 0.35 33.7 ± 3.1 4.9 ± 0.45 The control 60.2 ± 2.4 36.7 ± 2.5 17.5 ± 3.0 9.8 ± 0.5 35.7 ± 5 2.04 ± 0.2 30 days after drug administration Experience 65.8 ± 3.6 42.4 ± 2.9 14.9 ± 2.0 8.5 ± 0.8 34.1 ± 4.3 3.97 ± 0.6 The control 60.7 ± 5.4 37.0 ± 0.5 17.2 ± 0.9 9.8 ± 0.4 35.8 ± 1.5 2.06 ± 0.1

Table 8 Biochemical and hematological blood parameters of sheep after the use of the drug for the treatment and prevention of diseases associated with selenium deficiency for farm animals at a dose of 1.17 mg / kg DV (n = 5) Group Glucose, mmol / L Hemoglobin, g / l Color indicator Red blood cells, mln / μl White blood cells, thousand / μl The activity of catalase, μm N ₂ O ₂ / l · min · 10 ³ Peroxidase activity, units opt. pl. / l sec MDA, μM / L Before drug administration Experience 4.60 ± 0.5 103.6 ± 7.6 0.95 ± 0.12 9.26 ± 0.65 9.3 ± 0.7 14.4 ± 2.4 25.1 ± 9.0 1.5 ± 0.7 The control 4.23 ± 0.7 107.3 ± 8.2 0.95 ± 0.17 9.54 ± 0.58 10.8 ± 2.4 14.7 ± 4.4 25.8 ± 10.0 1.61 ± 0.74 15 days after drug administration Experience 3.35 ± 0.19 110.3 ± 4.2 0.84 ± 0.09 11.1 ± 0.74 13.1 ± 0.8 26.0 ± 3.1 45.6 ± 3.2 0.48 ± 0.1 The control 4.09 ± 0.5 104 ± 12.7 0.93 ± 0.06 9.43 ± 0.46 10.7 ± 1.9 15.5 ± 2.6 25.7 ± 11.1 1.55 ± 0.61 30 days after drug administration Experience 3.29 ± 0.14 107.2 ± 4.8 0.81 ± 0.06 11.26 ± 0.25 11.9 ± 0/76 25.1 ± 0.89 43.3 ± 2.8 0.36 ± 0.04 The control 4.07 ± 0.31 103.6 ± 6.8 0.87 ± 0.06 10.0 ± 0.11 10.2 ± 1.45 17.2 ± 0.76 27.2 ± 8.8 1.49 ± 1.5

The advantages of the proposed drug is that it contains vitamin C and selenium in the complex - natural antioxidants, which are complementary, as well as a powerful immunostimulant - levamisole. As a result, the complex preparation has a pronounced antioxidant and immunostimulating effect. Neutrophils enriched with ascorbic acid enhance their ability to recognize and destroy (usually by phagocytosis) precancerous cells, bacterial, viral and other foreign agents. Most intensely, neutrophils absorb vitamin C during the “breathing explosion” necessary for the biosynthesis of bactericidal free radical substances (See Moser R., Weber F. Uptake of ascorbic acid by human granulocytes // Internal. J. Vit. Nutr. Res. - 1983 . - 54. - P.47.).

The invention in comparison with the prototype and other technical solutions has the following technical advantages:

- increase the overall resistance of the animal organism;

- increase antioxidant action;

- highly effective immunostimulant;

- low economic costs.

Claims (1)

A drug for the treatment and prevention of diseases associated with selenium deficiency for farm animals, including sodium selenite and water for injection, characterized in that it additionally contains levamisole base and ascorbic acid in the following ratio, wt.%:
sodium selenite 0.05-0.4 levamisole base 3.0-8.0 ascorbic acid 5.0-15.0 water for injections rest

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