RU2360434C2 - Method of obtaining biologically active preparation from cows colostrum - Google Patents

Abstract

FIELD: chemistry. ^ SUBSTANCE: invention relates to producing of biologically active products, which are used in live stock farming. Method of obtaining biologically active preparation from colostrum includes collecting of summer colostrums, filtering and freezing in freezing chambers at temperature -20 - -22C. Then colostrum is defrosted at 35 - 38C. Using rennet enzyme - pepsin in amount 4-6 g of pepsin per 1 l of colostrums, from colostrums is casein is precipitated. Colostrum whey is filtered from casein clots. Obtained in precipitating colostrums mass is dried at +50, crashed to powdery condition. Then into formed colostrum powder-like preparation dihydroquercetin is introduced - in amount 0.1 mg of dihydroquercetin per 1kg. ^ EFFECT: invention allows obtaining product with increased biological activity, which is used for increasing immunity and safety of chicken-broilers. ^ 1 dwg, 1 tbl

Description

The facts of obtaining colostrum preparations, which were tested on broiler chickens, are known. The basis of such drugs is casein, the use of which is known in animal husbandry.

There is a method for producing casein from milk using rennet, including precipitation, filtering casein (Kugenev P.V., Barabanshchikov N.V. Dairy Workshop. - M.: Agropromizdat, 1998. - p. 37).

The proposed method differs from the known one in that summer colostrum is used.

The method is as follows.

Fresh colostrum of the first milk yield of cows is collected from clinically healthy animals in farms that are safe for infectious diseases.

Colostrum is filtered, poured into sterile containers of 1.0-1.5 L and frozen in a freezer at a temperature of -20 - -22 ° C. Under these conditions, colostrum is stored for 4-8 months.

Colostrum is thawed in batches gradually at a temperature of +35 - + 38 ° С.

Using rennet pepsin, colostrum is precipitated from colostrum. To do this, thawed colostrum is heated in a water bath to +38 - + 40 ° С and dry pepsin powder is added at the rate of 4-6 g per 1 liter of colostrum. After mixing, the colostrum is precipitated. To form a denser clot and to separate it from serum, the mixture is placed in a cool place (up to 10 ° C) for 2-4 hours. The colostrum is separated from the clot of colostrum by filtration through a triple layer of gauze.

The colostrum is dried in an oven at a temperature of + 50 ° C. The resulting dry mass is placed in a homogenizer and ground to a powder state.

“Lavitol food” is introduced into the powder at the rate of 0.1 mg per 1 kg. The resulting preparation is Packed in sterile plastic bags and stored until use (5-10 days) in a refrigerator

0 - + 2 ° С.

The drug is a yellowish-white hygroscopic powder, poorly soluble in water and insoluble in alcohol and ether, with a specific smell of colostrum. It contains 8.0 mg% DNA, 35.7 mg% RNA, 37.61% total proteins, 17.4% total lipids, 6.3% carbohydrates, 6.01% vitamin A.

Example. On the basis of VNIMI (Petrov A.N., Rossikhina G.A., Turovskaya S.N. Preservation of cottage cheese on the basis of lyophilization // Dairy industry. - 2004. - No. 8. - S.32-35.) Milk was produced curd rennet-acid method. The resulting product was dried by freeze-drying. 0.015% quercetin and 0.02% ascorbic acid were added to the mass of fat in the product before drying. The product was packaged under nitrogen.

Unlike the specified method:

- used colostrum;

- clot dehydration was carried out without pressing through a gauze filter;

- in order to preserve useful whey proteins and other useful components, the curd mass was not washed;

- drying the freshly prepared mass was carried out in an oven on baking sheets with a layer of 5 mm thick at a temperature of + 50 ° C, stirring occasionally, for 18 ± 2 hours;

- the dry mass was ground in a homogenizer to a powder state;

- dihydroquercetin was used as an antioxidant at the rate of 0.1 mg per 1 kg of a colostrum preparation;

- packaged the drug in plastic bags of 100 g.

Experimental studies were carried out on broiler chickens in the spring of 2005. By the principle of analogues, 2 groups of chickens of 80 heads each were formed. In the experimental group, the chickens received a colostrum preparation together with the main diet in the amount of 18.75 g per 1 kg of body weight. The control group received only the main diet used in the production of the poultry farm. Broilers arrived at the day-old for breeding from the hatchery and were housed in three-tier cell batteries of the KBU-3 brand with 27 animals each. In the room where the chickens were kept, the optimal microclimate was maintained. The clinical condition of the experimental bird and the feed intake were regularly monitored. Chicks aged 5 and 30 days were bled for analysis. Feeding and preventive measures were carried out in accordance with the farm broiler chicken rearing scheme.

The change in the protein and nucleic acid composition of the blood of broiler chickens (drawing) confirms that the colostrum preparation affects the protein indicators of the blood of chickens already on the fifth day of feeding. The synthesis of serum blood proteins in experimental chickens is intensive on all days of the experiment, while in the control bird the content of serum proteins in the blood is noticeably lower. The research results showed that the blood of control and experimental chickens contains different amounts of nucleic acids. In chickens treated with the drug, the total nucleic acid content is significantly higher on all days of the experiment compared with the control.

The effects of the colostrum preparation on the viability of broiler chickens are shown in the table.

During the observation period (42 days) in the control and experimental groups were sick and dead chickens. However, when using the drug, the safety of chickens was 96.25%, against 93.75% in the control group.

The effectiveness of the use of the drug is determined by several factors.

Freezing is the most gentle form of conservation, in which almost all components of colostrum are preserved. In the process of thawing raw materials, the bactericidal effect of the drug is formed. Preservative - “Lavitol food” is a natural substance obtained by a special technology without the use of a chemical reagent. The active substance of this drug is dihydroquercetin-3,3 ', 4', 5,7'-pentahydroxyflavanone - a flavonoid of the antioxidant group of vitamins P. Today it is one of the most powerful antioxidants that works at the level of cell membranes. The basis for its use was the complete absence of any harmful properties and high antimicrobial and antifungal activity. Dihydroquercetin does not change the organoleptic properties of the drug, does not have toxicity and does not detect carcinogenic properties. It is able to increase the immunological reactivity and detoxification ability of the body (Komov V.P. Biochemistry. - M.: Drofa, 2004).

The effect of the drug on the safety of broiler chickens Indicators Groups of chickens Control Experienced Ill,% 2.5 1.25 Fell,% 6.25 3.75 Preservation,% 93.75 96.25

Consequently, the use of a colostrum drug for prophylactic purposes stimulates the chickens' own immune defense forces and reduces their mortality. The cooking technology is cheap, available for use in poultry farms. The method of obtaining the drug is environmentally friendly, allows you to save biologically active substances and does not introduce reagents hazardous to the health of birds in raw materials.

Claims (1)

A method of producing a biologically active preparation from colostrum, including sedimentation of the colostrum, filtering off the colostrum and drying it at a temperature of 50 ° C, characterized in that the colostrum obtained in the summer is frozen at (-20) - (-22) ° C, then if necessary, the colostrum is thawed at 35-38 ° С and pepsin is added at the rate of 4-6 g per 1 liter of colostrum, and the obtained colostrum mass is dried, ground to powder, canned with dihydroquercetin at the rate of 0.1 mg per 1 kg of the preparation.

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