RU2356557C2 - Homeopathic medicine for treatment and prevention of endometritis in animals - Google Patents

Abstract

FIELD: veterinary.

SUBSTANCE: invention relates to medications, which are applied in obstetric-gynecological practice, namely for treatment and prevention of endometritis in animals. Homeopathic medicine contains components with following ratio, ml: Endometrium bovis D16 10; Silver metallic D29 10; Quartz D29 10; - Narrow-leaf Coneflower D2 10; mountain arnica D2-D4 10; Ethanol- rectified product 18-20; Solution of sodium chloride 0.9% to 100.

EFFECT: invention ensures increase of therapeutic effect and term of medication use.

6 tbl, 3 ex, 2 dwg

Description

The proposed homeopathic remedy "Endometricomp" refers to medicines used in obstetric and gynecological practice, namely for the treatment and prevention of animal endometritis.

Known homeopathic remedy "Mastometrin" used in the treatment of acute and chronic endometritis of animals (see Lebedeva N.V. Treatment of endometritis in dogs with homeopathic medicines // Veterinary clinic, February 2004, Yekaterinburg. - P.8-9). "Mastometrin" is also used for vaginitis, dysmenorrhea, mastitis, retention of the placenta, eclampsia, because its components (Pulsatilla D15, ASD-2 D9, Lachesis mutus D8; Sepia D6; Sabina D3) have an anti-inflammatory effect, positively affect reparative processes, activate tissue immunity and increase the body's resistance (http: //dolina.sitecity. ru). However, it does not have a specific effect on the uterine mucosa.

As a prototype, a potentized homeopathic remedy of the PlantaVet firm (Germany) Endometrium Compositum was selected and tested (see Yepanchintseva OS, Yeremeyeva AG Diagnosis and treatment of cows with hidden endometritis // Actual problems of veterinary medicine in modern conditions: Materials of the international scientific-practical conference dedicated to the 60th anniversary of the Krasnodar NIVI. - Krasnodar, 2006. - P.344-347).

"Endometrium compositum" is a homeopathic remedy that is used to treat acute and chronic diseases of the uterine mucosa. Composition: Endometrium G1 D16 10 ml, Argentum metallicum D29 10 ml, Quarz D29 10 ml, Echinacea angustifolia planta tota D2 10 ml, isotonic sodium chloride solution up to 100 ml.

The bovine endometrium is the lining of the uterus of a healthy cow. Its action is due to the pathogenetic effect and is aimed at mobilizing and stimulating the body's defenses to eliminate the pathological process in the uterus. A targeted effect on pathogenesis is accompanied by a weakening of the influence of the etiological factor. The bovine endometrium comprehensively affects the body in various ways: humoral, through the central and autonomic nervous system, by activating immunobiological protection, and also stimulates the restoration of pathologically altered uterine mucosa.

Silver metal is a widespread element, the solutions of which have a bactericidal effect. The mechanism of action of silver on microorganisms is the inactivation of certain enzymes, that is, it acts as an enzyme poison. The saturation of water with silver ions is strictly dosed, since silver is toxic in large doses. The bactericidal effect of silver depends not only on its concentration, but also on the nature of the medium: it is the same at a silver nitrate concentration of 1: 5,000,000 in distilled water and 1: 80,000 in blood serum. Silver is a part of homeopathic remedies in high dilutions and is used with equal effectiveness in the treatment of acute and chronic diseases.

Quartz - SiO ₂ - one of the most common minerals, has well-defined piezoelectric properties. Quartz has a wide therapeutic range, a positive effect on the course of chronic diseases.

Echinacea narrow-leaved contains active substances (polysaccharides, essential oil, echinacoside glycoside, betaine, resins, phytosterols, organic acids: palmitic, linoleic, cherotin), which enhance the body's defenses and act as non-specific stimulants. Active substances inhibit the growth of pathogens by increasing the number of granulocytes and activating phagocytosis. In addition to antibacterial action, echinacea has an antiviral and antimycotic effect, reduces the body's sensitivity to stressful effects.

"Endometrium compositum" is available in 50 ml bottles, does not contain preservatives, therefore its use is limited. After violating the integrity of the cork during the collection of the drug, the solution must be used for a short time (no more than 1 month), otherwise the drug loses its sterility. In the treatment of small animals, a small dose of the drug is used, so the use of a large bottle is not economically justified. "Endometrium compositum" - a drug of selective action on the uterine mucosa, which has anti-inflammatory, antimicrobial, analgesic and immunostimulating effects. However, Endometrium Compositum does not have a hemostatic effect, which is important in the treatment of endometritis that occurs with damage to the vessels of the uterine wall, accompanied by bleeding.

The aim of the invention is to increase the therapeutic effect and the term of use of the drug.

This goal is achieved in that the homeopathic remedy "Endometricomp" additionally contains mountain arnica D2-D4 and ethyl alcohol 18-20%.

The proposed homeopathic remedy has the following composition.

Bovis Endometrium D 16 ml 10 Silver metal D29, ml 10 Quartz D29, ml 10 Echinacea narrow-leaved D2, ml 10 Arnica mountain D2-D4, ml 10 Ethyl rectified spirit, ml 18-20 Sodium Chloride Solution 0.9%, ml up to 100

Mountain arnica flowers contain up to 4% arnitsin, up to 0.15% essential oil, 4-5% tannins, organic acids (fumaric, malic, lactic), 19-21 mg /% ascorbic acid, up to 2.5% fructose, up to 1% sucrose, gum, inulin, proteins, chlorophylls, carotenoids, cinarin, choline, betaine, alkaloids. The healing effect of arnica is due to the presence of a variety of biologically active substances. Arnica has a pronounced stimulating effect on blood coagulation and hemostasis. Essential oil exhibits a bactericidal property, and in combination with carotenoids promotes the regeneration of damaged tissues. In addition to hemostatic and anti-inflammatory effects, arnica strengthens the tone and contractions of the uterus. In the form of tincture, arnica is used for subinvolution, acute and chronic inflammatory diseases of the uterus, for the prevention of pyemia.

Ethyl alcohol is added as a preservative. We have experimentally established that its presence does not affect the therapeutic and prophylactic effect, however, it significantly increases the shelf life and use of a homeopathic remedy after breaking the integrity of the cork, which is especially important when working with small doses.

The literature describes a method for preparing homeopathic remedies (Klenova I.O., Yaremenko N.A. Veterinary preparations in Russia: A Handbook. - M.: Selkhozizdat, 2000. - P.480-482), according to which raw materials for their preparation can be medicinal plants, minerals, metals, inorganic and organic acids, as well as vitamins, enzymes and animal tissues. The starting materials (essences, tinctures, solutions) are mixed with a carrier substance and potentiated. Mixing with a carrier substance (water, milk sugar) is carried out at each stage in accordance with 1 + 9 = 10 (decimal scale, D) or 1 + 99 = 100 (hundredth scale, C). This method allows you to minimize the dose and at the same time increase its activity due to the treatment of the drug (dilution with shaking). The dosage form obtained in this way has the optimal healing property in the absence of side effects and is called potency, and the working process of preparing such drugs is called potentiation. As a result of these manipulations, a persistent change in the structure of the carrier (water or milk sugar) occurs, which does not change during further stages of potentiation. The prepared first dilution (D1 or C1) is then used to obtain a dilution of D2, D3 or C2, SZ, etc. Therefore, a quantitative assessment of a homeopathic remedy is characterized not by concentration, but by potency or dilution, while a qualitative assessment is due to the physicochemical properties of water, changed in accordance with the structure of the active principle. Depending on the degree of dilution, the drug may affect various levels of the pathological process. Low dilutions up to D12 are indicated in acute reactive; medium - up to D30 - with subacute and chronic; above D30 - in atypical chronic processes.

Example No. 1. Determination of the degree of dilution of the tinctures of Arnica montana in the composition of the "Endometricomp.

In the experiment to determine the degree of dilution of the tincture of Arnica montana as part of the Endometricomp, the course of the pathological process in endometritis (acute and exacerbation of chronic) was taken into account. Animals of different types (cattle, dogs and cats) with catarrhal-hemorrhagic endometritis were selected, of which six groups of five animals each were formed. Experimental animals were injected with a test drug, which included tincture of Arnica mountain in various dilutions: D2, D3, D4, D6, D8, D10. Observation of the animals was carried out during the entire course of treatment on the basis of a clinical examination, gynecological examination, and data from hematological studies. We took into account the general condition of the animal, the number and nature of the outflows, the dynamics of hematological parameters: the number of red blood cells in 1 mm ^{3 of} blood, the erythrocyte sedimentation rate (ESR), the level of hematocrit (Ht) and the hemoglobin content (Hb). The control group consisted of clinically healthy animals.

As a result of the study, it was found that in the dilution of arnica D10, the drug did not have a hemostatic effect, i.e. in vaginal bleeding, blood was noted for 7-8 days. In the first 3-4 days of treatment, there was a negative dynamics in the values of hematological parameters: acceleration of ESR by 35-52% (figure 1), a decrease in the number of red blood cells by 8.7-9.5%, hemoglobin by 18-25% (figure 2 ) and hematocrit by 5.9-8.5%. Gradual stabilization and restoration of these indicators to a physiological norm occurred, starting from 8-10 days of treatment. By the 10th day of treatment, a decrease in ESR by 9.4-10.6% was recorded, by the 20th day - by 66.7-68% (Fig. 1), an increase in the number of red blood cells - by 1.8-2%, respectively and 4.8-5.6%. The hemoglobin content increased by the 20th day of treatment by 9.2-10.6% (figure 2), hematocrit - by 5.9-6.2%.

A moderate hemostatic effect was expressed in the dilution of arnica D8 and D6. Impurities of blood in vaginal outflows disappeared by the 5-6th day of treatment. Accordingly, hematological parameters were restored faster. So, starting from 6-8 days of treatment, a decrease in ESR was recorded, by the 10th day of treatment, ESR decreased by 30-34%, and by the 20th day - by 78-83% (Fig. 1). An increase in the number of red blood cells was noted by the 10th day of treatment by 3.7-4%, by the 20th day - by 8.2-9%, hemoglobin - by 2.4-4% and 18-23%, respectively (Fig. 2), hematocrit - by 5.8-6.3% and 6.6-7.4%.

The therapeutic hemostatic effect of Arnica was observed in its dilution of D2, D3 and D4, which was expressed in the decrease and disappearance of blood impurities in vaginal outflows by the 3-4th day of treatment, as a result of which the improvement in blood counts was noted from 4-5 days of treatment. By the 10th day of treatment, a decrease in ESR was recorded by 44-48%, by the 20th day - by 83-87% (Fig. 1), an increase in the number of red blood cells - by 7.5-8.2% and 15-17, respectively , 2%, hemoglobin - by 7.6-8.9% and 26-28.4% (figure 2), hematocrit - by 3.8-4.6% and 12.7-13.8%. Thus, breeding arnica D2-D4 is optimal.

Example No. 2. The experimental justification for adding tincture of mountain arnica as a hemostatic in the homeopathic remedy "Endometricomp".

In addition to mountain arnica, we tested such medicinal plants as dioecious nettle, common yarrow, and highlander pepper (water pepper) as a hemostatic agent in Endometricomp. For this, dogs with acute purulent-hemorrhagic endometritis were injected with test agents containing extracts of various plants in a D2-D4 dilution. As a result, four groups of animals, five animals each, were formed by the principle of analogues. During the entire course of treatment, animals were monitored and hematological studies were performed.

The study found that the average duration of endometritis treatment in the first group was 8.17 days, i.e. 1.5-2 days less than other groups (table. No. 1).

Table 1 Duration of treatment of acute endometritis in dogs Groups 1st 2nd 3rd 4th The medicinal plant that is part of the product Arnica mountain Stinging nettle Yarrow Highlander pepper The average duration of treatment, days 8.17 ± 0.31 10.83 ± 0.48 11.17 ± 0.48 9.5 ± 0.43

From table No. 2 it is seen that as a result of treatment, the restoration of hematological parameters (ESR, the number of red blood cells and leukocytes, hemoglobin and hematocrit) was faster in the first group than in the other groups. In the animals of the first group, in the first two days of treatment, the uterine tone increased without the use of contractions, then a decrease in the number of outflows was observed; blood impurities in the expiration stopped 2-4 days earlier.

table 2 Dynamics of hematological parameters Group number Days of treatment ESR, mm / h The number of leukocytes, × 10 ¹² / l The number of red blood cells, × 10 ⁹ / l Hemoglobin, g / l Hematocrit% Before treatment 31.63 ± 1.26 23.82 ± 1.19 5.02 ± 0.16 96.71 ± 2.62 37.29 ± 0.84 one 10 16.15 ± 1.13 14.24 ± 0.56 5.54 ± 0.33 110.31 ± 1.76 38.86 ± 1.23 twenty 6.19 ± 1.16 9.43 ± 0.29 6.19 ± 0.21 130.17 ± 1.25 42.14 ± 0.96 2 10 26.63 ± 0.89 16.34 ± 0.24 5.31 ± 0.16 95.13 ± 1.21 36.94 ± 0.87 twenty 10.24 ± 1.36 12.41 ± 0.16 5.56 ± 0.26 116.35 ± 1.14 39.12 ± 0.96 3 10 28.19 ± 1.24 16.7 ± 0.38 5.26 ± 0.21 97.32 ± 1.04 36.78 ± 0.78 twenty 12.47 ± 1.14 12.92 ± 0.76 5.48 ± 0.16 118.85 ± 1.07 39.03 ± 0.63 four 10 20.16 ± 1.39 15.24 ± 0.36 5.42 ± 0.18 104.58 ± 1.16 37.81 ± 0.95 twenty 8.27 ± 1.54 11.73 ± 0.42 5.87 ± 0.22 122.37 ± 1.0 41.36 ± 1.21 Clinically Healthy Animals 1.67 ± 0.12 8.72 ± 0.2 6.86 ± 0.11 146.67 ± 1.23 48.36 ± 1.27

Thus, unlike other tested medicinal plants, arnica has not only hemostatic, but also a reducing and anti-inflammatory effect, and also enhances the antimicrobial effect of echinacea, which is part of the drug.

Example No. 3. Determination of alcohol concentration in the composition of the homeopathic remedy "Endometricomp".

To determine the optimal concentration of alcohol in the composition of the "Endometricomp" conducted clinical trials means with different alcohol content. For this, 12 experimental clinically healthy animals were injected subcutaneously with Endometricomp with 10, 14, 18, 20, 22, 26, and 30% alcohol concentrations, taking into account pain during administration, the appearance of edema, hyperemia, compaction in the injection area, and an increase local and general temperature. The test results are shown in table No. 3.

Table 3 Indicators The concentration of alcohol in the "Endometricomp",% 10 fourteen eighteen twenty 22 26 thirty Soreness when administered - - - - ± + + Edema and hyperemia - - - - - ± + Sealing at the injection site - - - - ± + + Rise in local temperature - - - - - ± + Short-term increase in body temperature - - - - - - +

The table shows that the drug with an alcohol concentration of 20% and below did not cause irritation and inflammation of the surrounding tissues with subcutaneous administration. With the introduction of funds with 22% or higher alcohol content, tissue irritation was noted, accompanied by soreness, compaction and fever.

The term of use of a homeopathic remedy with an alcohol concentration of 18-20% is extended to a year. This concentration is optimal.

To determine the therapeutic efficacy of Endometricomp, animals with acute and chronic endometritis were selected from which three groups of 30 animals each were formed (10 cats, 10 dogs and 10 cows). The tests were carried out in the clinic of the Institute of Veterinary Medicine of the Omsk State Agrarian University, the Veterinary Center of LLC Petan and the farms of the Omsk Region (SPK Pushkinskoye, ZAO Azovskoye, ZAO named after Kirov and others) during 2004-2006. In the treatment regimen of animals of the first group, the tested drug “Endometrcomp” was included as a main therapy in a dose of 1-10 ml (depending on the type of animal) subcutaneously once every 48 hours with a course of up to 10 injections. The animals of the second group were treated with the homeopathic remedy “Endometrium compositum” in a similar course. The animals of the third group used the traditional method of treatment using antibiotics. Animals of all groups were injected subcutaneously with oxytocin, intrauterine antimicrobial and multivitamin preparations for 4-5 days. Throughout the course of treatment, in the absence of pain, the uterus was massage through the abdominal wall to small domestic animals and through the rectum of the cows. The effectiveness of the treatment was evaluated on the basis of a clinical examination, the results of a microbiological analysis of cervical-vaginal mucus, data of morphological and biochemical blood tests. Observation of the animals was carried out during the next two sexual cycles, taking into account the nature of the vaginal mucus during oestrus, the occurrence or absence of relapse, the number of days of infertility and the insemination index were also taken into account in cows.

As a result of the studies, it was found that in the first and second group, a noticeable improvement in the general condition was observed after 3-4 days from the start of treatment: the appearance of appetite, a decrease in body temperature to normal, a decrease in uterine pain during palpation. It was noted that in the first two days of treatment, the uterus was cleansed - the number of secretions increased, and then gradual enlightenment and a decrease in the number of outflows were observed, and in animals of the first group, blood impurities in the outflows stopped 2-4 days earlier. In animals of the third group, an improvement in the general condition and a decrease in the number of outflows were observed on the 5-6th day of treatment.

From table No. 4 it is seen that the average duration of treatment of acute endometritis with the proposed agent in the first experimental group was 8 days in dogs, in cats - 6 days, chronic - 18 and 17 days, respectively, which is 3-5 days less than in the second group , and for 5-7 days than in the third. In 90% of recovered animals, restoration of the reproductive cycle without recurrence of the disease was recorded; in relapses (10%), a subsequent course of treatment was subsequently successfully conducted. Seven dogs and cats of the third group, after 10-60 days after the end of treatment, resumed expiration, as a result of which he underwent ovariogysterectomy.

Table 4 Comparative efficacy of endometritis therapy in domestic carnivores Indicators 1st group "Endometkomp" 2nd group “Endometrium compositum” 3rd group traditional therapy Dogs Cats Dogs Cats Dogs Cats He recovered without relapse,% 80 90 80 80 60 fifty The number of relapses,% 10 10 10 twenty thirty 40 Fell,% 10 - 10 - 10 10 The average duration of treatment of acute endometritis, days 8 6 eleven 8 fifteen eleven The average duration of treatment of chronic endometritis, days eighteen 17 22 22 23 23

Thus, the therapeutic efficacy of the Endometkomp homeopathic remedy in the treatment of domestic carnivore endometritis was to shorten the treatment period by 3-5 days compared to the prototype, as well as to restore 90% of the reproductive function of recovered animals and to avoid surgical intervention.

From table No. 5 it is seen that in the cows of the first experimental group using "Endometricomp" the average duration of treatment of acute endometritis was 5.6 days; chronic - 32.7; hidden - 9, which is 4-12 days less than in the second group, and 12-19 days less than in the third. The number of days of infertility decreased in acute endometritis by 2.4 times compared with the second and 14.4 times compared with the third group, and the insemination index - by 0.2 and 0.9, respectively. In chronic endometritis, the number of days of infertility decreased by 1.2 times compared to the second and 1.5 times compared to the third group, and the insemination index - by 0.4 and 1.7, respectively. With latent endometritis, the number of days of infertility decreased by 1.3 times compared to the second and 2.3 times compared to the third group, and the insemination index - by 0.2 and 2.5, respectively.

Table 5 Comparative effectiveness of various treatment regimens for endometritis in cows Diagnosis Indicators 1st group "Endometrikomp" 2nd group “Endometrium compositum” 3rd group traditional therapy Acute endometritis The average duration of treatment, days 5,6 9.6 eighteen Infertility days 2.7 6.6 39 Insemination index 1,1 1.3 2 Chronic endometritis The average duration of treatment, days 32,7 38,2 46 Infertility days 60 73,4 89.5 Insemination index one 1.4 2.7 Latent endometritis The average duration of treatment, days 9 21 28 Infertility days 72 94 168 Insemination index 1,5 1.7 four

Thus, the therapeutic effect of the proposed homeopathic remedy "Endometkomp" in the treatment of cow endometritis was to reduce the treatment period by 1.5-2 times, the number of days of infertility by 1.2-2.3 times, the insemination index by 0.2-0, 4, as well as in 100% recovery of the reproductive function of recovered animals.

For the experiment to determine the effectiveness of the prevention of postpartum endometritis, four groups of 20 animals each (10 cats and 10 dogs) were formed after conservative obstetric care with pathological delivery and cesarean section. Animals of all groups were injected subcutaneously with oxytocin, multivitamin preparations for 3-4 days, antimicrobials were administered intrauterine, and in the absence of pain, the uterus was massaged through the abdominal wall. The animals of the first (experimental) group used the tested homeopathic remedy “Endometricomp” with a course of 3-5 subcutaneous injections. The animals of the second group used Endometrium Compositum in a similar course. The animals of the third group were additionally treated with general antibiotic therapy. Animals of the fourth group used only local antibiotic therapy.

Table 6 Comparative effectiveness of various endometritis prevention regimens Groups 1st group 2nd group 3rd group 4th group The number of cases, goal 0 0 0 7 The effectiveness of pharmacoprophylaxis,% one hundred one hundred one hundred 65 Duration of postpartum uterine involution, days 11.2 ± 0.73 13.0 ± 0.58 16.1 ± 0.6 19.5 ± 0.65 Side effects Absent. Absent. digestive disorders, decreased immunity, dysbiosis in young animals Absent.

The effectiveness of prevention was assessed by the presence of clinical signs of physiological involution of the uterus and the absence of signs of endometritis (depression, fever, decreased or lack of appetite, profuse catarrhal-hemorrhagic or purulent outflows with an unpleasant odor from the vulva).

From table No. 6 it is seen that the prevention of postpartum endometritis in animals using potentiated homeopathic remedies (group 1 and 2) was more effective and did not cause side effects. With the use of Endometricomp, the terms of postpartum uterine involution were reduced on average by 15-20%.

Claims (1)

Homeopathic remedy for the treatment and prevention of animal endometritis, containing bovine endometrium, metallic silver, quartz, narrow-leaved echinacea, characterized in that it additionally contains mountain arnica in breeding D2-D4 and ethyl alcohol 18-20% in the following ratio of components, ml:
Bovis Endometrium D16 10 Silver metal D29 10 Quartz D29 10 Echinacea narrowleaf D2 10 Arnica mountain D2-D4 10 Rectified ethyl alcohol 18-20 Sodium Chloride Solution 0.9% up to 100

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