RU2330886C2 - NEW FLUORESCENT PROTEINS Aequorea coerulscens AND METHODS OF APPLICATION THEREOF - Google Patents
NEW FLUORESCENT PROTEINS Aequorea coerulscens AND METHODS OF APPLICATION THEREOF Download PDFInfo
- Publication number
- RU2330886C2 RU2330886C2 RU2004125585/13A RU2004125585A RU2330886C2 RU 2330886 C2 RU2330886 C2 RU 2330886C2 RU 2004125585/13 A RU2004125585/13 A RU 2004125585/13A RU 2004125585 A RU2004125585 A RU 2004125585A RU 2330886 C2 RU2330886 C2 RU 2330886C2
- Authority
- RU
- Russia
- Prior art keywords
- nucleic acid
- cell
- polypeptide
- protein
- fluorescent
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims abstract 6
- 102000034287 fluorescent proteins Human genes 0.000 title abstract 5
- 108091006047 fluorescent proteins Proteins 0.000 title abstract 5
- 241000243290 Aequorea Species 0.000 title 1
- 108020004707 nucleic acids Proteins 0.000 claims abstract 20
- 102000039446 nucleic acids Human genes 0.000 claims abstract 20
- 150000007523 nucleic acids Chemical class 0.000 claims abstract 20
- 108090000623 proteins and genes Proteins 0.000 claims abstract 8
- 102000004169 proteins and genes Human genes 0.000 claims abstract 7
- 238000002372 labelling Methods 0.000 claims abstract 6
- 241001531066 Aequorea coerulescens Species 0.000 claims abstract 3
- 238000004519 manufacturing process Methods 0.000 claims abstract 3
- 230000001105 regulatory effect Effects 0.000 claims abstract 3
- 230000002068 genetic effect Effects 0.000 claims abstract 2
- 230000003834 intracellular effect Effects 0.000 claims abstract 2
- 230000002103 transcriptional effect Effects 0.000 claims abstract 2
- 230000009261 transgenic effect Effects 0.000 claims abstract 2
- 108090000765 processed proteins & peptides Proteins 0.000 claims 17
- 229920001184 polypeptide Polymers 0.000 claims 12
- 102000004196 processed proteins & peptides Human genes 0.000 claims 12
- 125000003275 alpha amino acid group Chemical group 0.000 claims 5
- 230000004927 fusion Effects 0.000 claims 3
- 239000002773 nucleotide Substances 0.000 claims 3
- 125000003729 nucleotide group Chemical group 0.000 claims 3
- 238000006467 substitution reaction Methods 0.000 claims 3
- 230000007762 localization of cell Effects 0.000 claims 2
- 102220549285 CBY1-interacting BAR domain-containing protein 1_F64L_mutation Human genes 0.000 claims 1
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 1
- 108700026244 Open Reading Frames Proteins 0.000 claims 1
- 108010076504 Protein Sorting Signals Proteins 0.000 claims 1
- 108020001507 fusion proteins Proteins 0.000 claims 1
- 102000037865 fusion proteins Human genes 0.000 claims 1
- 102220306159 rs185266383 Human genes 0.000 claims 1
- 102200054160 rs28941775 Human genes 0.000 claims 1
- 230000015572 biosynthetic process Effects 0.000 abstract 1
- 239000003814 drug Substances 0.000 abstract 1
- 230000000694 effects Effects 0.000 abstract 1
- 238000010353 genetic engineering Methods 0.000 abstract 1
- 239000000126 substance Substances 0.000 abstract 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
- C07K14/43595—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from coelenteratae, e.g. medusae
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
- A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Toxicology (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Gastroenterology & Hepatology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
FIELD: medicine.
SUBSTANCE: invention refers to genetic engineering and biotechnology and can be used for labelling of proteins, cells and organisms. Substitutes added to nucleic acid Aequorea coerulescens, coding colourless GFP-like protein acGFP is considered as means of production of nucleic acid coding fluorescent protein. Expression cassette containing this nucleic acid under regulatory elements control provides in-cell biosynthesis of fluorescent protein. This genetic structure is used for production of transgenic organism and cell producing fluorescent protein, coded by specified nucleic acid. This fluorescent protein is applied in methods for labelling of cells, intracellular structure and protein, as well as for promotor transcriptional activity registration.
EFFECT: invention application ensures widen range of labelling means used in biochemistry, molecular biology and medical diagnostics.
11 cl, 34 dwg, 2 ex
Description
Claims (11)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US35151802P | 2002-01-22 | 2002-01-22 | |
US60/351,518 | 2002-01-22 |
Publications (2)
Publication Number | Publication Date |
---|---|
RU2004125585A RU2004125585A (en) | 2005-04-20 |
RU2330886C2 true RU2330886C2 (en) | 2008-08-10 |
Family
ID=27613506
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
RU2004125585/13A RU2330886C2 (en) | 2002-01-22 | 2003-01-17 | NEW FLUORESCENT PROTEINS Aequorea coerulscens AND METHODS OF APPLICATION THEREOF |
Country Status (12)
Country | Link |
---|---|
US (4) | US7432053B2 (en) |
EP (1) | EP1485481B1 (en) |
JP (3) | JP4510464B2 (en) |
AT (1) | ATE458816T1 (en) |
AU (1) | AU2003208520B2 (en) |
CA (1) | CA2474108C (en) |
DE (1) | DE60331422D1 (en) |
DK (1) | DK1485481T3 (en) |
ES (1) | ES2340154T3 (en) |
IL (2) | IL162568A0 (en) |
RU (1) | RU2330886C2 (en) |
WO (1) | WO2003062270A2 (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2010107405A1 (en) * | 2009-03-20 | 2010-09-23 | Razumenko Mihail Viktorovich | Method for producing peptides which specifically recognize cells of particular types and can be used for therapeutic purposes |
RU2602452C2 (en) * | 2014-05-14 | 2016-11-20 | Федеральное государственное бюджетное учреждение науки Институт биологии гена Российской академии наук | Method of substances and their compositions detection with anticancer activity |
Families Citing this family (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE60331422D1 (en) * | 2002-01-22 | 2010-04-08 | Evrogen Ip | FLUORESCENT PROTEIN FROM AEQUOREA COERULESCENS AND ITS USES |
ATE474922T1 (en) | 2002-12-26 | 2010-08-15 | Zakrytoe Aktsionernoe Obschest | FLUORESCENT PROTEINS FROM COPEPODA SPECIES AND METHOD FOR USE THEREOF |
EP1820024B1 (en) | 2004-12-01 | 2012-06-13 | Proteologics, Ltd | Ubiquitin ligase assays and related reagents |
US8268550B2 (en) * | 2009-06-26 | 2012-09-18 | Massachusetts Institute Of Technology | Compositions and methods for identification of PARP function, inhibitors, and activators |
WO2010151664A2 (en) | 2009-06-26 | 2010-12-29 | Massachusetts Institute Of Technology | Compositions and methods for treating cancer and modulating stress granule formation |
US8435961B2 (en) | 2009-06-26 | 2013-05-07 | Massachusetts Institute Of Technology | Methods and compositions for increasing the activity of inhibitory RNA |
KR101800878B1 (en) * | 2010-03-31 | 2017-11-27 | 전남대학교 산학협력단 | Methods for Analyzing a Genotoxicity Using Non-fluorescent Fluorescence Proteins |
WO2011135040A1 (en) | 2010-04-30 | 2011-11-03 | F. Hoffmann-La Roche Ag | Fluorescent antibody fusion protein, its production and use |
US9134301B2 (en) * | 2010-11-22 | 2015-09-15 | Bio-Rad Laboratories, Inc. | Sorting of adherent cells by selective transformation of labels |
US9040248B2 (en) | 2012-06-06 | 2015-05-26 | The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Kits for detecting and monitoring endocrine disrupting chemicals (EDCs) |
US20140024066A1 (en) * | 2012-06-20 | 2014-01-23 | California Institute Of Technology | Animal model having photo-activatable mitochondria |
JPWO2022215532A1 (en) * | 2021-04-07 | 2022-10-13 | ||
CN116023460B (en) * | 2022-10-28 | 2023-11-10 | 无锡佰翱得生物科学股份有限公司 | Staygold yellow fluorescent protein and application thereof |
Family Cites Families (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5777079A (en) * | 1994-11-10 | 1998-07-07 | The Regents Of The University Of California | Modified green fluorescent proteins |
US5625048A (en) * | 1994-11-10 | 1997-04-29 | The Regents Of The University Of California | Modified green fluorescent proteins |
US5668750A (en) * | 1995-07-28 | 1997-09-16 | Nec Corporation | Bipolar multiplier with wide input voltage range using multitail cell |
US5874304A (en) * | 1996-01-18 | 1999-02-23 | University Of Florida Research Foundation, Inc. | Humanized green fluorescent protein genes and methods |
US6096865A (en) * | 1996-05-06 | 2000-08-01 | Amgen Inc. | Mutants of the green fluorescent protein having improved fluorescent properties at 37° |
WO2000034326A1 (en) * | 1998-12-11 | 2000-06-15 | Clontech Laboratories, Inc. | Fluorescent proteins from non-bioluminescent species of class anthozoa, genes encoding such proteins and uses thereof |
KR100842969B1 (en) * | 2000-12-01 | 2008-07-01 | 쓰리엠 이노베이티브 프로퍼티즈 컴파니 | Crosslinked Pressure Sensitive Adhesive Compositions, Adhesive Articles Based Thereon, Useful in High Temperature Applications, and a Process for Protecting a Substrate Using the Article |
GB0109858D0 (en) * | 2001-04-23 | 2001-06-13 | Amersham Pharm Biotech Uk Ltd | Fluorscent proteins |
DE60331422D1 (en) * | 2002-01-22 | 2010-04-08 | Evrogen Ip | FLUORESCENT PROTEIN FROM AEQUOREA COERULESCENS AND ITS USES |
-
2003
- 2003-01-17 DE DE60331422T patent/DE60331422D1/en not_active Expired - Lifetime
- 2003-01-17 JP JP2003562147A patent/JP4510464B2/en not_active Expired - Fee Related
- 2003-01-17 EP EP03706812A patent/EP1485481B1/en not_active Expired - Lifetime
- 2003-01-17 ES ES03706812T patent/ES2340154T3/en not_active Expired - Lifetime
- 2003-01-17 RU RU2004125585/13A patent/RU2330886C2/en active
- 2003-01-17 DK DK03706812.9T patent/DK1485481T3/en active
- 2003-01-17 CA CA2474108A patent/CA2474108C/en not_active Expired - Lifetime
- 2003-01-17 AU AU2003208520A patent/AU2003208520B2/en not_active Expired
- 2003-01-17 WO PCT/IB2003/000907 patent/WO2003062270A2/en active Application Filing
- 2003-01-17 US US10/501,629 patent/US7432053B2/en not_active Expired - Lifetime
- 2003-01-17 AT AT03706812T patent/ATE458816T1/en not_active IP Right Cessation
- 2003-01-17 IL IL16256803A patent/IL162568A0/en unknown
-
2004
- 2004-06-16 IL IL162568A patent/IL162568A/en not_active IP Right Cessation
-
2008
- 2008-08-28 US US12/199,983 patent/US7667016B2/en not_active Expired - Lifetime
- 2008-08-28 US US12/200,064 patent/US7897726B2/en active Active
- 2008-08-28 US US12/200,113 patent/US7879988B2/en not_active Expired - Lifetime
-
2009
- 2009-10-22 JP JP2009243614A patent/JP2010046084A/en active Pending
-
2010
- 2010-11-05 JP JP2010248679A patent/JP5465649B2/en not_active Expired - Lifetime
Non-Patent Citations (2)
Title |
---|
GREEN-FLUORESCENT PROTEIN. * |
Proteins. 2000 Dec 1; 41(4):429-37. FEBS Lett. 1995 Jun 26; 367(2):163-6. Nat Biotechnol. 1999 Oct; 17(10):969-73. Nucleic Acids Res.2000 August 15; 28(16):e78. * |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2010107405A1 (en) * | 2009-03-20 | 2010-09-23 | Razumenko Mihail Viktorovich | Method for producing peptides which specifically recognize cells of particular types and can be used for therapeutic purposes |
RU2602452C2 (en) * | 2014-05-14 | 2016-11-20 | Федеральное государственное бюджетное учреждение науки Институт биологии гена Российской академии наук | Method of substances and their compositions detection with anticancer activity |
Also Published As
Publication number | Publication date |
---|---|
WO2003062270A8 (en) | 2004-11-04 |
AU2003208520B2 (en) | 2008-06-05 |
EP1485481B1 (en) | 2010-02-24 |
IL162568A0 (en) | 2005-11-20 |
US20090148939A1 (en) | 2009-06-11 |
JP2005526495A (en) | 2005-09-08 |
US20090137033A1 (en) | 2009-05-28 |
ATE458816T1 (en) | 2010-03-15 |
US7897726B2 (en) | 2011-03-01 |
WO2003062270A3 (en) | 2003-11-27 |
DK1485481T3 (en) | 2010-06-07 |
JP2010046084A (en) | 2010-03-04 |
WO2003062270B1 (en) | 2004-04-01 |
CA2474108A1 (en) | 2003-07-31 |
US20090148940A1 (en) | 2009-06-11 |
US7879988B2 (en) | 2011-02-01 |
ES2340154T3 (en) | 2010-05-31 |
US20060167225A1 (en) | 2006-07-27 |
RU2004125585A (en) | 2005-04-20 |
JP2011087581A (en) | 2011-05-06 |
JP5465649B2 (en) | 2014-04-09 |
DE60331422D1 (en) | 2010-04-08 |
WO2003062270A2 (en) | 2003-07-31 |
EP1485481A2 (en) | 2004-12-15 |
US7667016B2 (en) | 2010-02-23 |
JP4510464B2 (en) | 2010-07-21 |
US7432053B2 (en) | 2008-10-07 |
CA2474108C (en) | 2012-06-19 |
IL162568A (en) | 2010-12-30 |
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