WO2003040312A3 - Multifunctional green fluorescent protein and uses thereof - Google Patents

Multifunctional green fluorescent protein and uses thereof Download PDF

Info

Publication number
WO2003040312A3
WO2003040312A3 PCT/US2002/034448 US0234448W WO03040312A3 WO 2003040312 A3 WO2003040312 A3 WO 2003040312A3 US 0234448 W US0234448 W US 0234448W WO 03040312 A3 WO03040312 A3 WO 03040312A3
Authority
WO
WIPO (PCT)
Prior art keywords
green fluorescent
fluorescent protein
protein
multifunctional green
multifunctional
Prior art date
Application number
PCT/US2002/034448
Other languages
French (fr)
Other versions
WO2003040312A2 (en
Inventor
Wei Wen Su
Robert C Bugos
Rosanto I Paramban
Original Assignee
Univ Hawaii
Wei Wen Su
Robert C Bugos
Rosanto I Paramban
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Hawaii, Wei Wen Su, Robert C Bugos, Rosanto I Paramban filed Critical Univ Hawaii
Priority to AU2002363466A priority Critical patent/AU2002363466A1/en
Publication of WO2003040312A2 publication Critical patent/WO2003040312A2/en
Publication of WO2003040312A3 publication Critical patent/WO2003040312A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43595—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from coelenteratae, e.g. medusae
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Abstract

The present invention relates to a multifunctional green fluorescent protein engineered to have a purification tag inserted into a β-barrel structure of the green fluorescent protein molecule, and nucleic acid constructs, host cells, expression systems, and fusion proteins containing the multifunctional green fluorescent protein. The present invention also relates to microarrays having a tagged-green fluorescent protein. Also disclosed are methods for purifying a protein or polypeptide, identifying expression of a protein or polypeptide in biological material, and identifying a target molecule using a multifunctional green fluorescent protein of the present invention.
PCT/US2002/034448 2001-10-24 2002-10-24 Multifunctional green fluorescent protein and uses thereof WO2003040312A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002363466A AU2002363466A1 (en) 2001-10-24 2002-10-24 Multifunctional green fluorescent protein and uses thereof

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US34783301P 2001-10-24 2001-10-24
US60/347,833 2001-10-24
US37169102P 2002-04-10 2002-04-10
US60/371,691 2002-04-10

Publications (2)

Publication Number Publication Date
WO2003040312A2 WO2003040312A2 (en) 2003-05-15
WO2003040312A3 true WO2003040312A3 (en) 2004-10-28

Family

ID=26995438

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/034448 WO2003040312A2 (en) 2001-10-24 2002-10-24 Multifunctional green fluorescent protein and uses thereof

Country Status (2)

Country Link
AU (1) AU2002363466A1 (en)
WO (1) WO2003040312A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7591040B2 (en) 2003-12-18 2009-09-22 Kimberly-Clark Worldwide, Inc. Cleaning tool for removing larger and smaller sized particles
CN106492770A (en) * 2015-09-08 2017-03-15 中国科学院大连化学物理研究所 A kind of histidine-tagged protein affinity purification material and its application
CN109060744B (en) * 2018-08-14 2020-02-18 华南理工大学 Fluorescence analysis method for secretion expression quantity of pichia pastoris recombinant protein
CN109134637B (en) * 2018-09-26 2021-08-31 生工生物工程（上海）股份有限公司 Method for purifying fluorescent protein

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6319669B1 (en) * 1994-11-10 2001-11-20 The Regents Of The University Of California Modified green fluorescent proteins
US6403374B1 (en) * 1996-08-16 2002-06-11 The Regents Of The University Of California Long wavelength engineered fluorescent proteins

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6319669B1 (en) * 1994-11-10 2001-11-20 The Regents Of The University Of California Modified green fluorescent proteins
US6403374B1 (en) * 1996-08-16 2002-06-11 The Regents Of The University Of California Long wavelength engineered fluorescent proteins

Also Published As

Publication number Publication date
AU2002363466A1 (en) 2003-05-19
WO2003040312A2 (en) 2003-05-15

Similar Documents

Publication Publication Date Title
WO2005100395A3 (en) EXPRESSION SYSTEM FOR PREPARING IL-15/Fc FUSION PROTEINS AND ITS USE
WO1999064592A3 (en) Green fluorescent proteins for measuring the ph of a biological sample
WO2003020932A1 (en) Novel secretory proteins and dna thereof
WO2002070652A3 (en) Novel molecules of the card-related protein family and uses thereof
WO2005003315A3 (en) Compositions and methods for peptide-assisted transfection
WO2002022662A3 (en) Insect inhibitory bacillus thuringiensis proteins, fusions, and methods of use therefor
WO2003008449A8 (en) Ntb-a, a surface molecule involved in natural killer cells activity
WO2003040312A3 (en) Multifunctional green fluorescent protein and uses thereof
WO2002097041A3 (en) Fusion proteins of biologically active peptides and antibodies
WO2001073028A3 (en) A p-selectin glycoprotein ligand (psgl-1) binding protein and uses therefor
WO2002068459A3 (en) Non aggregating fluorescent proteins and methods for using the same
EP2354156A3 (en) Nucleic acids and proteins of insect OR83B odorant receptor genes and uses thereof
WO2002033084A3 (en) Identification of the dombrock blood group glycoprotein as a polymorphic member of the adp-ribosyltransferase gene family
WO1999054437A3 (en) Novel molecules of the t125-related protein family and uses thereof
WO2006097336A3 (en) A novel component of the wg/wnt signaling pathway
WO2001092493A3 (en) 21956 and 25856, human aminopeptidases and uses thereof
WO2005023981A3 (en) Libraries of chimeric cellulose binding proteins and methods of use thereof
WO2003012121A3 (en) Collagen xxii, a novel human collagen and uses thereof
WO2000055192A3 (en) Hemocyanin and nucleic acid sequence coding for the same
WO2004029276A3 (en) Nucleic acids encoding a mammalian raptor polypeptide and uses therefor
WO2003020912A3 (en) Novel clark protein and nucleic acid molecules and uses therefor
WO2003031590A3 (en) Nucleic acids encoding linked chromo/fluorescent domains and methods for using the same
WO2004065554A3 (en) Human saa3 nucleic acid molecule, protein, and methods of use for same
WO2001083523A3 (en) Stmst protein and nucleic acid molecules and uses therefor
WO2001059065A3 (en) Novel molecules of the card-related protein family and uses thereof

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ OM PH PL PT RO RU SD SE SG SI SK SL TJ TM TN TR TT TZ UA UG US UZ VC VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR IE IT LU MC NL PT SE SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
122 Ep: pct application non-entry in european phase
NENP Non-entry into the national phase

Ref country code: JP

WWW Wipo information: withdrawn in national office

Country of ref document: JP