RU2290958C1 - Device for removing leucocytes from blood products - Google Patents

Abstract

FIELD: medicine.

SUBSTANCE: device can be used for removing of leucocytes from donor's blood or from its components, namely, erythrocyte-mass and plasma. Device has filter and connecting tubes. There are protrusions on case and cap of device to place inlet and outlet openings for fluid. Protrusions are provided with connecting members. Connecting tubes are connected to inlet and outlet openings for fluid by means of two-step bushing. Two cylinders of different sizes are made outside bushing. Cylinder with bigger diameter is connected with connecting member, disposed inside openings of protrusions of case and cap. Cylinder of smaller diameter is connected with connecting tubes, which has internal diameter to be smaller than internal diameter of bushing. Glue is not used for fastening.

EFFECT: reliable fastening of tubes to filter; reduced cost of device.

4 cl, 2 dwg

Description

The invention relates to medicine, more specifically to transfusion, and can be used in the process of processing blood for subsequent storage or infusion. The device is designed to remove white blood cells from donated blood or its components: erythromass and plasma, in order to prevent immunological reactions and complications, as well as to prevent a number of infectious diseases, prevent the formation of microclots and improve storage conditions of red blood cells. The device can find application at blood transfusion stations, donor points, in medical institutions.

Known filter device for removing white blood cells [European patent No. 155003 A2], comprising a housing and a cover with a filter material located in the cavity between the housing and the cover. On the housing and the cover of the device protrusions are made to accommodate the inlet and outlet openings for liquids, equipped with connecting elements. In this case, the inlet is connected by means of tubes and a tee to containers for blood and an anticoagulant, and the outlet is connected by means of a tube to a container for collecting leukemic blood. Sealing the inner cavity of the filter, and therefore the filter material, is done by gluing or welding the housing and cover. An adhesive joint is used to connect the filter to the tubes of the blood supply line and the anticoagulant.

The disadvantage of this device is that it is impossible to provide a reliable tight connection of the tubes and the filter without the use of glue, the cost of such a product is quite high, since expensive materials are used, in particular polycarbonate, which can be glued and welded, but is difficult to process.

In addition, the assembly of the known filter presents certain difficulties and belongs to the category of harmful production, since it uses gluing operations.

A device for removing viral pathogens from the blood according to the application of the Russian Federation No. 98114098 A1, including a filter housing, a cover and filter material. In the housing and the cover there is a liquid inlet connected to an external line connection means, a liquid outlet connected to a container for collecting the filtered product, and an air vent, in addition, the device includes a tube bypassing the filter with the filter installed it has a one-way valve designed to vent air from the container to collect the filtered blood product. The tubes are connected to the filter by gluing.

However, this device also has the same disadvantages: when connecting the tubes to the filter, toxic glue is used, expensive, difficult to process materials.

Currently, the removal of leukocytes from the blood and its components is one of the most effective ways to prevent well-known immunological reactions and complications, as well as the transfer of viruses associated with white cells and a number of other infections. In this regard, the need for filtering devices is increasing. Accordingly, they are subject to requirements, not only regarding the provision of high filtering properties, but also requirements of an economic and technological nature. For a widespread and widespread implementation of the filtration procedure, it is necessary to reduce the cost of the product, to ensure manufacturability and safety of its assembly and use. Compliance with these conditions should not lead to a deterioration of the operating parameters of the filter, i.e. decrease in filtering ability.

The removal of white blood cells from blood transfusion media using white blood filters produced by foreign manufacturers has now become a mandatory procedure in transfusion practice of developed foreign countries. Until the end of the last century, leukofiltration was practically unavailable for the domestic blood service due to the high cost of imported filtering devices.

Therefore, the task was to create a cheaper reliable domestic technological device for filtering blood and its products by removing gels, microaggregate particles and leukocytes that carry viral pathogens, which will prevent pyrogenic reactions and reduce the risk of graft versus host disease and the transmission of a number of viral infections.

The technical result, which the claimed invention is directed to, is to ensure reliable tight fastening of the tubes to the filter without the use of glue, and therefore, to increase the reliability of the device as a whole and the filtering procedure.

An additional technical result is to reduce costs through the use of materials (polyethylene, polypropylene) with low cost (instead of expensive and difficult to process polycarbonate used abroad), which are practically not amenable to welding and gluing, but due to their low cost and manufacturability, they reduce production time parts i.e. reduce the cost of the product and increase labor productivity.

The technical result is achieved due to the fact that in the device for purification of blood and its products, including connecting tubes and a filter, on the body and cover of which there are protrusions for placing the inlet and outlet openings for liquids equipped with connecting elements, the connecting tubes are connected to the input and output liquid holes with a two-stage sleeve, on the outside of which two cylinders of different diameters are made, a cylinder of a larger diameter is connected to the connecting element, located inside the holes of the protrusions of the cover and the housing, and a cylinder of a smaller diameter is connected to the connecting tube, the inner diameter of which is less than the outer diameter of the sleeve.

The two-stage sleeve is made of hard material, and the filter housing and cover with protrusions located on them and connecting tubes are made of elastic.

The larger cylinder has a sharp annular edge, and the connecting elements are cylindrical holes with an annular protrusion, while the sharp annular edge of the sleeve is located below the annular protrusion of the cylindrical hole.

An additional protrusion is made in the lid or housing to accommodate the air hole, also provided with a connecting element.

The invention will be more clear from the description with reference to the position of the drawings, where Fig. 1 shows a diagram of a device for cleaning blood products, Fig. 2 shows a sectional view of a filter protrusion with connecting elements.

The device 1 includes a connector 2 for sterile connection, tees 3, clamps 4, connecting tubes 5 and a filter 6. The filter 6 has a housing 7 and a cover 8. On the housing 7 and the cover 8 of the filter 6 protrusions 9, 10 are made to accommodate the input 12 and output 13 fluid holes. Between the housing 7 and the cover 8 placed filter material.

An additional protrusion 11 is made on the lid 8 or the housing 7 of the filter 6 to accommodate the air hole 14. The holes for liquid and air are provided with connecting elements. The connecting tubes 5 are connected to the inlet 12 and the outlet 13 for the liquid holes and the air hole 14 using a two-stage sleeve 15, on the outside of which two cylinders of different diameters are made, a cylinder of a larger diameter 16 is connected to the connecting element located inside the holes of the protrusions of the cover 8 and the housing 7, and a cylinder of a smaller diameter 17 is connected to the connecting tube 5, the inner diameter of which is less than the outer diameter of the sleeve 15.

The two-stage sleeve 15 is made of hard material, and the housing 7 and the cover 8 of the filter 6 with the protrusions 9-11 located on them and the connecting tubes 5 are made of elastic. This allows you to tightly connect the tube 5 with the sleeve 15, without using glue or welding.

The cylinder of larger diameter 16 has a sharp annular edge 18, and the connecting elements are cylindrical holes 19 with an annular protrusion 20, while the sharp annular edge 18 of the sleeve 15 is located behind the annular protrusion 20 from the inlet of the cylindrical hole 19. Due to this embodiment of the connected parts, a high reliable connection between the filter 6 and the bushings 15 without the use of glue or welding.

The liquid inlet 12 through the corresponding tubes 5, tee 3 and roller clip 4 is connected to the connector 2. The roller clip 4 at the inlet of the device is used to smoothly control the speed of the transfused plasma or erythromass, since the filtration quality and the capture rate of leukocytes and other filtered particles are also determined and feed rate of the filtered medium. A tube, a second tee, a tube with a first clamp connected to a first container for a leukocyte-free plasma 21. are connected in series to the second inlet of the tee 3. The second outlet of the second tee is connected to the air hole 14 by a tube with a second clamp 14. The liquid outlet 13 of the filter 6 is connected to a third tee, the first outlet of which is connected by a tube with a third clamp to a container for a leukocyte-free plasma 21. The second outlet of a third tee is connected by a tube with a fourth clamp to a second container 22 for a profile Trovan product: bezleykotsitnoy red blood cell.

The device creates a closed system when it is connected to containers with previously separated blood components from a single donor (plasma and red blood cells) due to connector 2, which is specially designed to provide a connection that guarantees the preservation of the sterility of the transfused components.

The device is assembled and operates as follows.

The bushings 15 of hard material are pressed into the holes 19 of the protrusions 9-11 of the housing 7 and the cover 8 of the filter 6, which, as mentioned earlier, are made of elastic material. Connecting tubes 5 with clamps mounted on them are put on cylinders of a smaller diameter 17 of the bushings 15, the tubes 5, in addition, are connected to the corresponding tees 3, connector 2 and containers 21, 22 for filtered blood products. The device is ready to go.

Before filtration, the suitability of the filtered media — plasma and erythromass — is checked; the shelf life of preserved blood and its products before filtration should not exceed 48 hours at 4 ° C. Device 1 is removed from the package. Close all clamps 4. Containers with filtered media are hung to a height of at least 1.5 m with a vertical filter. In this case, the container with the plasma should be 0.2 m lower than the container with the erythrocyte mass. To displace air from the filter 6, it has an outlet 13 for the liquid up, open the corresponding clamps 4. Under the action of gravity, the filter is filled 6. The plasma filtering process continue until its entire volume is filtered out of the plasma container. At the end of plasma filtration, close the roller clip 4.

To filter the erythromass, it is transferred from the erythrocyte mass container to the plasma container by removing the corresponding clip. Open the roller clamp 4 and displace the remaining plasma from the tubes 5 and the filter 6 into a container for a leukocyte-free plasma 21. Then the container for a leukocyte-free plasma 21 is placed in a plasma extractor (not shown). Using a plasma extractor, they press on the container for a leukocyte-free plasma 21, the remaining erythromass from the tubes 5 and filter 6 (from the inlet side) are pressed into the container for leukocyte-free erythromycas with air collected in it 22. Open the second clamp and again gently press on the container for a leukocyte-free plasma 21. The collected air transfers the remaining erythromass from the filter 6 (from the inlet side) and tubes 5 into the container for leukocyte-free erythromass 22. Open the clamp and gently press on the container again I have a leukocyte-free plasma 21. The remaining air in it transfers the residues of filtered erythromass from filter 6 and tubes 5 into a container for leukocyte-free erythromass 22. Close the fourth clamp.

The device according to the claimed invention has the following advantages:

A reliable tight connection of the connecting tubes to the filter is carried out.

They use cheap easily processed materials - polyethylene, polypropylene, which are not amenable or difficult to weld and glue, but due to their manufacturability they can reduce the manufacturing time of filter parts and reliably connect parts without using glue and welding.

The cost of the device is reduced through the use of cheap polymer materials.

Better working conditions are ensured by eliminating the use of toxic adhesives.

Claims (4)

1. A device for cleaning blood and its products, including connecting tubes and a filter, on the housing and the cover of which there are protrusions for placing the inlet and outlet openings for liquids equipped with connecting elements, characterized in that the connecting tubes are connected to the inlet and outlet openings for liquid using a two-stage sleeve, on the outside of which two cylinders of different diameters are made, a larger diameter cylinder is connected to a connecting element located inside the holes ystupov cover and housing, and the cylinder of smaller diameter is connected to the connection tube having an inside diameter smaller than the outer diameter of the sleeve. 2. The device according to claim 1, characterized in that the two-stage sleeve is made of hard material, and the housing and the filter cover with protrusions located on them and the connecting tube are made of elastic. 3. The device according to claim 1, characterized in that the cylinder of larger diameter has a sharp annular edge, and the connecting elements are cylindrical holes with an annular protrusion, with a sharp annular edge located below the annular protrusion of the cylindrical hole. 4. The device according to one of claims 1 to 3, characterized in that an additional protrusion is made in the lid or casing to accommodate the air hole, also provided with a connecting element.

Images