JP2001276181A - Blood bag with unnecessary matter removal filter - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a blood bag with an unnecessary matter removal filter making it possible to recover, through a simple operation, blood that can be preserved for a long time, from which unnecessary matter has been removed. SOLUTION: The blood bag with an unnecessary matter removal filter includes the unnecessary matter removal filter 10, a blood sampling bag 20, a recovery bag 30, and a blood inflow tube 40 and a blood outflow tube 50 which connect them together. A branch tube 52 is installed in a portion of the blood outflow tube 50 and a bypass tube 60 communicating with the blood sampling bag 20 is provided to extend from the branch tube 52. The bypass the 60 is provided with a hydrophobic filter 70 which is permeable to gas while impermeable to blood.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

The present invention relates to a method for removing at least one of unnecessary substances such as leukocytes, platelets, microaggregates, viruses, bacteria, prions, and pathogenic substances from blood collected from a donor. Autoimmune disease to preserve for a certain period of time, or to remove at least one of unnecessary substances such as compounds and excess drug produced in the process for inactivating the virus from blood products and to preserve for a certain period of time, Removal of at least one of pathogenic substances such as autoantibodies, activated leukocytes, abnormally-proliferated excess plasma protein fractions, etc. from autologous blood transfusion blood collected from a patient, and removal of unnecessary substances used for storage for a certain period of time The present invention relates to a blood bag with a filter.

[0002]

2. Description of the Related Art Blood transfusions using blood collected from donors have been performed for the purpose of treating various diseases and improving pathological conditions.

[0003] Conventionally, blood (whole blood) collected from a donor has been stored as it is and used for transfusion. In recent years, such storage with whole blood and transfusion have been performed only for some purposes. In general, whole blood is separated for each predetermined blood component by a separation method using centrifugal force, etc.
They are stored under conditions suitable for each component, and only blood components required by patients are transfused. Typical conditions used for storage and transfusion of separated blood components include concentrated red blood cell components used to treat anemia and maintenance of hemoglobin concentration due to massive bleeding, and concentrated platelets used to treat thrombocytopenia There are components, plasma components used to improve or maintain circulating plasma volume reduction. In addition, there are albumin used for burns and hemorrhagic shocks based on plasma components, globulin used in combination with antibiotics for severe infections, and blood coagulation factor VIII used for hemophilia.

[0004] In order to use each blood component whenever necessary, it is required that the blood components can be stored for as long as possible as long as their clinical efficacy is maintained. Therefore, for example, in an anticoagulant, an ACD-A solution (aqueous solution containing sodium citrate, citric acid, and glucose) and a CPD solution (sodium citrate, citric acid, phosphoric acid) having a longer anticoagulation duration than heparin Aqueous solution containing sodium dihydrogen, dextrose). In addition, in the storage container for blood components, there is less risk of breakage than glass bottles,
Plastic bags made of polyvinyl chloride or polyolefin with excellent gas permeability have been developed. Furthermore, in order to prevent bacteria from entering the blood components by opening to the atmosphere,
Due to the development of a closed bag system (closed system) in which several of the above-mentioned polyvinyl chloride bags are connected in an air-tight state, anticoagulants to be used, erythrocyte preservation solution, preservation containers, and blood by storage Depending on the regulations of each country regarding the properties of the components, the concentrated red blood cell component can be stored for 42 days, the concentrated platelet component can be stored for 5 days, and the plasma component can be stored for about one year by freezing.

On the other hand, these blood components for transfusion are generated by various undesired substances, for example, leukocytes mixed into each blood component in the process of separation from whole blood, and denatured coagulation factors when stored. Gels, microaggregates, viruses, bacteria, prions, endotoxins, etc. may be present in the mixture, and these unnecessary substances may cause problems in clinical practice. One or two or more are removed using an instrument such as a filter.

[0006] To give a typical example, at the time of blood transfusion, leukocytes mixed in blood components cause immunological side effects such as inconsistency of HLA type, and pathogens and the like incorporated into leukocytes of a donor. It is known that transmission of the virus causes an infection. Non-hemolytic pyrogenic reactions, platelet refractory reactions due to alloantibody production and side megavirus (CMV) infections.
The prion, which is a pathogen of Jacob disease, has been a problem due to the risk of being transmitted to patients by transfusion using leukocytes as a medium.

[0007] In order to prevent side effects caused by leukocytes mixed in these blood components for transfusion, a blood set provided with a leukocyte removal filter has been developed, and in recent years has been widely used due to its clinical value. In Europe, the Council of Europe's G
uide to the preparation, use and qualityassurance
450mL in of blood components 4th edition
The number of leukocytes mixed in one unit of blood component derived from blood collection is 1 × 1
0 defines ^six as below. In the United States, Amer
STANDA issued by icanassociation of blood banks
RDS for blood banks and transfusion services17th e
In the dition, the number of white blood cells mixed in one unit of a blood component derived from 450 mL of blood is set to be 5 × 10 ⁶ or less.

In a blood set provided with a leukocyte removal filter, a tube provided with a bottle needle at the tip thereof is provided upstream of the leukocyte removal filter, and the bottle needle is used to discharge a bag containing whole blood or blood components. It punctures the outlet, flows whole blood or blood components from the bag, and passes through a leukocyte removal filter to capture and separate leukocytes. For this reason, when a bottle needle is connected to the outlet of the bag, it becomes an open-to-atmosphere system (open system). Therefore, in consideration of the risk of invasion and propagation of bacteria and the like, blood from which leukocytes have been removed is usually used. The components are required to be transfused directly into the patient or transfused within 24 hours after leukapheresis. Therefore, the blood component from which leukocytes have been removed by such an open-to-atmosphere system cannot be stored for more than 24 hours.

In order to solve this problem,
For example, Japanese Patent Publication No. 6-59304, Japanese Patent Publication No. 6-59
No. 305 (US Pat. No. 4,985,153, EP 349,188) and an air-closed bag system and leukocyte used for separating each blood component from whole blood. An in-line filter system (blood bag with a leukocyte removal filter) in combination with a removal filter has been developed.

The in-line filter system includes a blood collection bag provided with a blood collection needle for collecting blood from a blood donor, a leukocyte removal filter for removing leukocytes from blood, and a collection for storing blood from which leukocytes have been removed. A bag and a separation bag for storing blood components are connected by a tube. However, in the in-line filter system as described above, the system is not opened to the atmosphere. (1) Since a bag (collection bag) for storing blood from which leukocytes have been removed contains almost no sterilized air. After the end of filtration,
Blood components other than leukocytes remaining in the tube connecting the blood collection bag and the blood inlet of the leukocyte removal filter, the housing of the leukocyte removal filter, and the filter medium cannot be collected.

(2) When blood flows into the leukocyte removal filter, in the tube connecting the blood collection bag and the leukocyte removal filter, in the housing of the leukocyte removal filter, or in the tube connecting the leukocyte removal filter and the collection bag. Large amount of sterilized air existing in the collection bag flows into the collection bag together with the blood after the filtration (after the leukocyte removal). Therefore, after the blood after filtration is centrifuged to separate each blood component, when the blood is removed from the centrifuge, the interface between the blood components is disturbed. Furthermore, when the supernatant component after centrifugation is transferred to another bag (storage bag) via a tube, the air itself is transferred to the storage bag at high speed because the flow resistance of the air itself is very small. In addition, the interface between the blood components in the collection bag rises rapidly, the interface fluctuates, and in the worst case, the blood components tend to be mixed before being transferred.

In order to solve these problems, there is provided a bypass tube connecting an upstream tube and a downstream tube of a leukocyte removal filter so as to bypass a leukocyte removal filter provided in an in-line filter system. Is disclosed in Japanese Patent Publication No. 4-504532 (International Publication WO 91/08820). Japanese Patent Application Laid-Open Publication No. Hei 8-500532 (International Publication) discloses a similar device, which is provided with a bypass tube connecting the upstream tube of the leukocyte removal filter and the collection bag so as to bypass the leukocyte removal filter. WO94 / 28996).

Here, a description will be given of an inline filter system provided with a bypass tube. The inline filter system (blood bag with leukocyte removal filter) is a blood collection bag provided with a blood collection needle for collecting blood from a donor. A leukocyte removal filter for removing leukocytes from blood and a collection bag for storing leukocyte-removed blood are connected by a tube, and further upstream of the leukocyte removal filter so as to bypass the leukocyte removal filter. A bypass tube is provided for connecting the side tube and the downstream tube or the collection bag.

According to such a blood processing method for a blood bag with a leukocyte removal filter, the blood in the blood collection bag is passed through a leukocyte removal filter through an upstream tube to remove leukocytes, and the blood from which leukocytes have been removed. Is collected in a collection bag via a downstream tube. Next, the air in the tube or the leukocyte removal filter that has flowed into the collection bag together with the blood from which leukocytes have been removed is transferred to the blood collection bag using a bypass tube. However,
Such an in-line filter system can transfer the air in the collection bag to the blood collection bag using the bypass tube, but even if the bypass tube is provided with a means for closing the flow path such as creme or the like. By mistake, blood before filtration flows into the bypass tube,
There was a risk of being stored in the collection bag.

To solve this drawback, a filter in which a hydrophobic filter is provided in the middle of a bypass tube that bypasses a leukocyte removal filter provided in an in-line filter system is disclosed in JP-A-8-50050.
No. 9 (International Publication WO94 / 05344). This hydrophobic filter can block blood that has entered the bypass line from the blood collection bag without passing through the leukocyte removal filter.

However, in any in-line filter system provided with a bypass tube,
(1) Since the air in the tube and the filter is temporarily stored together with the blood from which leukocytes have been removed in the collection bag, the capacity of the collection bag needs to be increased accordingly, resulting in an increase in waste. (2) If the volume of the collection bag is made larger than usual, when the blood in the collection bag is centrifuged, it is difficult to put the collection bag into the centrifugation cup, and the centrifugal separation has an excess volume. Therefore, it is not easy to remove the collection bag from the centrifuge cup while maintaining the separation interface between the blood components. (3) When transferring the air in the collection bag to the blood collection bag through the bypass tube, always position the air in the collection bag above the bag so that the blood after leukocyte removal is not discharged from the collection bag. Extreme caution is required.

(4) Further, after the leukocyte removal, blood remains in the tube between the leukocyte removal filter and the collection bag. Therefore, when the air in the collection bag is transferred to the bypass tube, the residual blood Since the flow resistance increases and the collection bag needs to be compressed with a strong force, the operation becomes complicated. In the case where a hydrophobic filter is provided in the middle of the bypass line, the blood remaining in the tube between the leukocyte removal filter and the collection bag must be collected in the collection bag using roller pliers. Since the blood reaches the hydrophobic filter and blocks the filter surface, the air in the collection bag cannot be transferred to the bypass tube, which further complicates the operation.

On the other hand, Japanese Patent Application Laid-Open No. H11-76397 discloses a blood set provided with the leukocyte removal filter described above, which is provided with a bypass tube for removing air in the tube and the filter. .
The blood set includes a bottle needle having two flow paths,
A bypass tube is provided for connecting the bottle needle to the downstream tube of the leukocyte removal filter.

However, as described above, the blood component from which leukocytes have been removed by the open-to-air system cannot be stored for more than 24 hours, so that an appropriate amount of blood cannot be transfused in an emergency. In addition, a bottle needle having two flow paths is an outlet of a bag containing blood components before removing white blood cells, usually a cylinder made of a soft polyvinyl chloride. The outer diameter of the bottle needle needs to be reduced to about 7 mm or less. If the outside diameter is larger than this, the puncture resistance will be too high, and it will not be possible to insert enough so as not to come off. For this reason, the two flow paths formed inside the bottle needle are inevitably very thin in consideration of the strength against the breakage of the bottle needle, and the passage resistance of the blood component increases, which is necessary. There is a serious problem that a sufficient flow rate cannot be obtained and there is a risk of activating blood passing therethrough. Further, molding and quality control of such a bottle needle having two flow paths are troublesome and labor intensive.

In the blood set disclosed in Japanese Patent Application Laid-Open No. 11-76397, the clamp provided in the tube communicating with the leukocyte removal filter is first opened for use. If the clamp provided on the bypass tube is opened first, blood flows into the hydrophobic filter provided on the bypass tube. In this case, since the entire surface of the hydrophobic filter is filled with blood, it is difficult to return all blood flowing into the hydrophobic filter to the blood collection bag. Also, even if some blood can be returned to the blood collection bag, air cannot flow from the blood collection bag to the collection bag via the bypass tube, so the blood flowing into the bypass tube is collected in the collection bag. There is also a problem that it cannot be done.

[0021]

SUMMARY OF THE INVENTION An object of the present invention is to provide a blood bag with an unnecessary substance removing filter which can recover blood from which unnecessary substances can be stored for a long period of time with a simple operation.

[0022]

The above-mentioned object is achieved by the blood bag with an unnecessary matter removing filter according to the present invention. The present invention relates to a first bag for storing blood containing unnecessary materials, a housing, and a filter for separating unnecessary materials provided to divide the inside of the housing into a blood inflow chamber and a blood outflow chamber. Waste removal filter,
A first tube having one end communicating with the first bag and the other end communicating with a blood inflow chamber in the housing, a second bag for containing blood from which unnecessary matter is separated, A blood bag with an unwanted matter removal filter, comprising: a second tube communicating with the second bag and the other end communicating with a blood outflow side in the housing, one end of which is a blood outflow side in the housing or A third tube communicating with the second tube and having the other end communicating with the first bag is provided. A hydrophobic filter that allows gas but does not allow blood is provided in the middle of the third tube. It is a blood bag with a filter for removing unnecessary substances.

It is preferable that the unnecessary matter is at least one of leukocytes, platelets, microaggregates, viruses, bacteria, prions, and pathogenic substances. Further, the hydrophobic filter includes a housing, and a filter provided so as to partition the inside of the housing into a blood inflow chamber and a blood outflow chamber, but allows gas to pass therethrough but does not allow blood to pass therethrough. Preferably, they are arranged. Preferably, the third tube extends into the first bag. Further, it is preferable that the inside of the first bag is partitioned into a connecting portion side of the first tube and a connecting portion side of the third tube. It is preferable that a seal portion extending into the first bag is provided between a connection portion of the first tube and a connection portion of the third tube of the first bag. Furthermore, it is preferable that a sealing member is provided near the connection between the first tube, the third tube, and the second tube with the third tube.

[0024] The present invention also provides a first bag for storing blood containing unnecessary substances, a housing, and a housing for dividing the interior into a blood inflow chamber and a blood outflow chamber. A filter that separates an object; a filter that separates the housing blood outflow chamber and a gas outlet of the housing; an unnecessary substance removal filter that includes a hydrophobic filter that allows gas to pass therethrough but does not allow blood to pass; A first tube communicating with the first bag and having the other end communicating with the blood inflow chamber in the housing; a second bag for storing blood from which unnecessary matter has been separated; A second tube communicating with the second bag and having the other end communicating with the blood outflow side in the housing; and a fourth tube having one end communicating with the gas outlet and the other end communicating with the first bag. Tube and body It is an unnecessary object removing blood bag with filter.

It is preferable that the unnecessary matter is at least one of leukocytes, platelets, microaggregates, viruses, bacteria, prions, and pathogenic substances. Further, it is preferable that the hydrophobic filter of the unnecessary substance removing filter is provided at an upper portion on the blood outflow side of the housing. Preferably, the fourth tube extends into the first bag. Further, it is preferable that the inside of the first bag is partitioned into a connection portion side of the first tube and a connection portion side of the third tube. Further, it is preferable that a sealing member is provided in the vicinity of a communicating portion of the first tube, the fourth tube, and the second tube with the fourth tube.

Further, it is preferable that a fifth tube having a blood collection needle at the tip is connected to the first bag. In addition, a separation bag for storing blood components
Is preferably connected to the second bag via the tube.

[0027]

BEST MODE FOR CARRYING OUT THE INVENTION The blood bag with an unnecessary substance removing filter according to the present invention can be used to remove unwanted substances, such as leukocytes, platelets, microaggregates, viruses, bacteria, prions, pathogenic substances, from blood or blood components. At least one of
It is applied to separate and store for a certain period of time. When the unnecessary matter is a leukocyte, it may be one that separates at least one of lymphocytes, granulocytes, and monocytes. Also,
If the unwanted matter is a virus, HAV, HBV, H
It isolates at least one of CV, HIV, HTLV-I, CMV, Parvovirus B19, Filou Louis, Hantavirus and the like.

In the present invention, the filter for removing unwanted substances that can be used for separating the target unwanted substances includes a leukocyte removal filter, a microaggregate (fine aggregate) removal filter, a virus removal filter, and the like.
Etc. Also, a plurality of these unnecessary substance removing filters can be combined or connected.

There are two types of leukocyte removal filters, one of which separates only white blood cells and the other of which separates white blood cells and platelets, and it is also possible to separate fine aggregates at the same time. Some microaggregate removal filters separate platelets in addition to those that separate only microaggregates. Some virus removal filters separate not only viruses but also endotoxins and / or microaggregates together, and can also selectively separate leukocytes and platelets. Some endotoxin removal filters separate not only endotoxin but also one or both of viruses and microaggregates, and can also selectively separate leukocytes and platelets.

As the leukocyte removal filter, it is possible to use a filter provided with a housing and a filter provided so as to divide the inside of the housing into a blood inflow chamber and a blood outflow chamber and separating at least white blood cells. The housing is formed from a rigid plastic material and has a blood inlet and a blood outlet. Further, as a filter for separating leukocytes, a three-dimensional mesh-like continuous porous body made of a material having low platelet adsorption can be used. Further, as a filter for separating leukocytes and platelets, a filter using a polyester nonwoven fabric as a prefilter and a laminate of a three-dimensional mesh-like continuous porous body made of a material having platelet adsorption properties as a main filter can be used.

The micro-aggregate removal filter includes a housing, and a filter provided so as to divide the inside of the housing into a blood inflow chamber and a blood outflow chamber and separating at least fine aggregates (microaggregates). Can be used. The housing is formed from a rigid plastic material and has a blood inlet and a blood outlet. In addition, as a filter for separating fine aggregates, a deep-capture type filter using a nonwoven fabric made of a thermoplastic resin can be used. Specifically, it is preferable to use a nonwoven fabric made of nylon.

As the virus removal filter, it is possible to use a filter provided with a housing, and a filter provided so as to divide the inside of the housing into a blood inflow chamber and a blood outflow chamber and separating at least viruses. The housing is formed from a rigid plastic material and has a blood inlet and a blood outlet. As a filter for separating viruses, a filter in which mannose-binding lectin is bonded to a hydrophobic polymer substrate formed on a porous body, woven fabric, nonwoven fabric, or the like via a hydrophilic polymer chain is used. Can be used. Specifically, a glycidyl methacrylate chain having an epoxy group is bonded to a polypropylene porous membrane via a methoxyethyl acrylate chain, and concanavalin A (ConA) is further bonded to an epoxy group.

Hereinafter, a blood bag with an unnecessary matter removing filter according to the present invention will be described in detail with reference to the preferred embodiment shown in the accompanying drawings. In addition, the case where a leukocyte removal filter is used as a representative example will be described as the waste removal filter, but the waste removal filter used in the blood bag with the waste removal filter of the present invention is not limited to the leukocyte removal filter. May be separated.

FIG. 1 is a circuit diagram showing a first embodiment of a blood bag with an unnecessary matter removing filter according to the present invention. As shown in the figure, the blood bag 1 with a leukocyte removal filter, which is a blood bag with an unwanted matter removal filter, is an unneeded matter removal filter (leukocyte removal filter) for capturing and separating leukocytes or leukocytes and platelets from blood collected from a donor. A filter) 10, a first bag (blood collection bag) 20 for containing blood collected from a donor, a second bag (collection bag) 30 for containing blood separated and captured leukocyte components, A first tube 40 for introducing the blood in the first bag 20 to an unnecessary matter removing filter (leukocyte removing filter) 10 and a blood in which leukocytes or leukocytes and platelets are captured and separated are introduced to a second bag 30. And the gas in the first tube 40 and the leukocyte removal filter 10 are transferred to the first bag 20. Third tube 60 to avoid the
And a hydrophobic filter 70 for blocking passage of blood and passing gas.

The hydrophobic filter 70 is provided in the middle of the third tube 60, and its position is located above the waste removal filter 10 (the first bag 20).
Side). In other words, it is located within the length range of the first tube 40 parallel to the third tube 60.

A blood collection needle 22 for collecting blood from a donor is connected to the first bag (blood collection bag) 20 via a fifth tube 21. The bag) 30 includes a separation bag 32 for storing blood components after centrifuging the blood in the second bag 30 and a storage solution bag 33 containing a storage solution for blood components. Sixth tube 3 with 34
1 are connected. And each tube has
A sealing member for closing and opening the blood flow is provided.

Specifically, the first tube 40 has the first
The sealing member 41 at a position close to the bag 20, the sealing member 51 at the second tube 50 on the downstream side of the branch tube 52 with the third tube 60, and the hydrophobic filter 70 at the third tube 60. Also, a sealing member 61 is provided at a position near the first bag 20, a sealing member 35 is provided at a position near the second bag 30 for the sixth tube 31, and a sealing member 36 is provided at a position near the storage liquid bag 33. Have been. The blood bag 1 with the leukocyte removal filter is sterilized by high-pressure steam sterilization (autoclave sterilization).

As shown in FIG. 1, the first
Bag 20, second bag 3 containing filtered blood
0, separation bag 3 containing blood components (eg, plasma)
Each bag of the preservation solution bag 33 that stores the preservation solution of the blood solution 2 and blood components (for example, red blood cells) will be described. Each bag is formed by laminating flexible sheet materials made of a soft resin such as polyvinyl chloride resin, and high frequency fusion, heat fusion or adhesion of the peripheral portion to form a bag. The inner portion surrounded by the seal portion is formed so that blood can be stored therein, and each bag has an internal volume suitable for the amount of blood to be stored.

Preferably, each bag is provided with one or more outlets (not shown) which are releasably sealed by peel tabs. In addition, the first bag 20
The blood collected from the donor using the blood collection needle 22 is stored through the fifth tube (blood collection tube) 21. Therefore, the first bag (blood collection bag) 20
ACD-A solution, CPD solution, C
An anticoagulant such as a PDA solution is pre-filled. Inside the storage liquid bag 33, a second bag (collection bag) 3
A preservative solution for preserving a specific blood component for a long time after centrifuging the blood in 0 is pre-filled. Specifically, MAP solution, SAGM solution, OP used for preserving red blood cells
It is filled with a red blood cell storage solution such as a TISOL solution.

First tube (blood inflow tube) 4
0, the second tube (blood outflow tube) 50, the third tube (bypass tube) 60, the fifth tube (blood collection tube) 21, and the sixth tube (connection tube) 31 are easy to handle. It is preferable to be formed of a soft polyvinyl chloride resin or a material mainly composed of a flexible polyvinyl chloride resin which has sufficient flexibility and flexibility when considered and has excellent compatibility with the sheet material of each bag when considering liquid tightness. . In addition, among the tubes, the third tube 60
Is preferably set smaller than the inner diameters of the other tubes.

Specifically, the inner diameter of each tube except for the third tube 60 is too small so as not to affect the blood passing therethrough such as activity, and it is too thick to be easily kinked or complicated to handle. In order to avoid
It is 2.5-4.0 mm, preferably about 3.0 mm. The inner diameter of the third tube 60 is 0.7 to 3.0 m.
m, preferably 1.0 to 2.0 mm. By doing so, the amount of blood flowing from the first bag 20 into the third tube 60 is reduced, which is convenient when blood is returned into the first bag 20 again. Further, since the flow rate of blood flowing from the first bag 20 into the third tube 60 is lower than the flow rate of blood flowing from the first bag 20 into the first tube 40, a third flow rate described later will be described. Filter 70 provided in the middle of the tube 60
Position can be easily set.

The length of each tube is 150 to 600 mm, preferably 200 to 200 mm.
About 350 mm, the second tube 50 is 150 to 100
0 mm, preferably about 200 to 800 mm, and the third tube 60 is 250 to 800 mm, preferably 300 to 800 mm.
About 550 mm, the fifth tube 21 is 800 to 180
0 mm, preferably about 1000 to 1600 mm.

Further, the branch pipes 34 and 5 shown in FIG.
Each of the branch pipes 2 is a T-shaped pipe molded using soft polyvinyl chloride. However, the branch pipes 34 and 52 in the present invention are not limited to this as long as they are three-way branch pipes.
The cross-shaped tube, the Y-shaped tube, or the inverted shape of the cross-shaped tube or the Y-shaped tube may be used.

Next, an example of the configuration of the unnecessary substance removing filter (white blood cell removing filter) 10 will be described. FIG.
It is a longitudinal section showing an example of composition of an unnecessary thing removal filter in the present invention. As shown in the figure, the leukocyte removal filter 10 which is an unnecessary substance removal filter is provided with a blood inlet 13.
And a filter 12 provided so as to divide the inside of the housing 11 into a blood inflow chamber 15 into which blood flows in and a blood outflow chamber 16 into which blood flows out. It is configured to flow into the blood inlet chamber 15 from the blood inlet 13, pass through the filter 12 without fail, and flow out of the blood outlet 14 via the blood outlet chamber 16.

Specifically, the peripheral portion of the filter 12 is liquid-tightly sandwiched between two members constituting the housing 11, a lid having a blood inlet 13 and a vessel having a blood outlet 14. The lid of the housing and the container are joined by ultrasonic fusion or the like. A first tube (blood inflow tube) 40 is connected to the blood inlet 13 in a liquid-tight manner, and a second tube (blood outflow tube) 50 is connected to the blood outlet 14 in a liquid-tight manner. It is configured as follows. Further, on the inner surface of the housing 11, a vertically long rib (not shown) for regulating the flow of blood and for holding down the filter 12 is provided, and is subjected to a hydrophilic treatment to prevent blood cells from adhering. You may. Housing 11
As a molding material of, hard plastic materials such as polycarbonate, polyolefin, and hard polyvinyl chloride resin can be suitably used. Further, it is preferable that the inner surface is subjected to antithrombotic treatment using heparin or the like.

As the filter 12, there are a type that passes red blood cells, plasma and platelets and removes only white blood cells when blood flows, and a type that passes red blood cells and plasma and removes white blood cells and platelets. The former type is preferably formed of a three-dimensional mesh-like continuous porous material having low platelet adsorption, such as polyether urethane and polysulfone, or a nonwoven fabric. Further, a material having a platelet-adsorbing property, for example, a polyester nonwoven fabric or the like may be coated with hydroxyethyl methacrylate or a copolymer of hydroxyethyl methacrylate and dimethylaminoethyl methacrylate.

On the other hand, as the latter type, a type composed of a laminate of a three-dimensional mesh-like continuous porous material having platelet adsorption properties can be used. Examples of the porous body having platelet adsorption properties include, for example, polycarbonate urethane, aliphatic polyamide, aromatic polyamide, polyester, and the like, and these may form a nonwoven fabric or a three-dimensional network continuous porous body. The surface of the porous body formed of the material having low platelet adsorption as described above is subjected to a cationization treatment,
A plate having a platelet adsorption property may be used. By adding platelet-adsorbing properties to such a low-platelet-adsorbing porous body, platelets are also trapped, and the leukocyte-trapping ability is further improved. Note that the cationization treatment is to chemically bond, coat, or contain a cationic compound on the surface of a substrate. Further, it is preferable to use such a porous body as a main filter and use a coarse nonwoven fabric or the like as a prefilter. For this prefilter, a polyester nonwoven fabric having a diameter of about 8 to 14 μm can be suitably used.

The filter 1 made of a porous material as described above
2 has a porosity of 75 to 95%, preferably 80 to 95%
It is. In addition, a single layer having a predetermined thickness may be used, but a flat layer having a thickness of about 0.2 to 10 mm and a stack of 2 to 8 sheets may be used. it can. When the porosity is 75% or more, the operation of removing leukocytes can be performed in a short time, and when it is 95% or less, there is no problem in the strength of the filter.

Next, a configuration example of the hydrophobic filter 70 will be described. FIG. 3 is a longitudinal sectional view showing a configuration example of the hydrophobic filter in the present invention. As shown in the figure,
The hydrophobic filter 70 includes a housing 71 having a gas inlet 73 and a gas outlet 74, and a filter 72 provided so as to divide the inside of the housing 71 into a gas inflow chamber 75 and a gas outflow chamber 76. Such a gas flows into the gas inflow chamber 75 from the gas inflow port 73, always passes through the filter 72, and flows out of the gas outflow port 74 through the gas outflow chamber 76. It is also possible for gas such as air to flow in the opposite direction, and air flows into the gas outflow chamber 76 from the gas outflow port 74, passes through the filter 72, passes through the gas inflow chamber 75, and flows through the gas inflow port 73.
Can be spilled from.

More specifically, the periphery of the filter 72 is liquid-tightly sandwiched between two members constituting the housing 71, a lid having a gas inlet 73, and a vessel having a gas outlet 74. The lid of the housing and the container are joined by ultrasonic fusion or the like.

As shown in the drawing, the filter 72 is disposed vertically (in a vertical state) in the housing 71, and the gas outlet chamber 76 and the gas outlet 74 are provided.
Are formed below the housing 71 (at the lower part in the direction of gravity) so as to join between the gas outlet chamber 76 and the gas outlet 74. Because of this structure,
The blood flowing from the first bag 20 into the third tube (bypass tube) 60 and reaching the hydrophobic filter 70 can be discharged into the first bag 20 by the air flowing from the gas inlet 73. . Further, the blood that has passed through the leukocyte removal filter 10 and has reached the hydrophobic filter 70 of the bypass tube 60 can be collected in the second bag 30 by the air that has flowed in from the gas outlet 74.

It is assumed that a filter 72 is provided in the housing 71.
Is installed horizontally, the first bag 2
It is extremely difficult to exhaust all the blood that has flowed into the bypass tube 60 from 0 into the first bag 20 by air. For this reason, the blood that has flowed into the bypass tube 60 after passing through the leukocyte removal filter 10 cannot be collected in the second bag 30 by air, and the collection rate of the filtered blood decreases. Further, even if the filter 72 is installed along the vertical direction in the housing 71, there is no wall that separates the gas outflow chamber 76 from the gas outflow port 74, and the gas outflow chamber 76 and the gas outflow port 74 are separated. Even in the case of being configured to merge above the housing 71 (upper portion in the direction of gravity), all blood flowing into the bypass tube 60 from the first bag 20 is discharged into the first bag 20 by air. It is difficult to do.

A gas inflow tube 601 which is a connection side of the bypass tube 60 with the blood outflow tube 50 is liquid-tightly connected to the gas inflow port 73, and a gas outflow port 74.
The gas outlet tube 602, which is the connection side of the bypass tube 60 with the blood collection bag 20, is liquid-tightly connected. Preferably, the gas outflow tube 602 is shorter than the gas inflow tube 601 and the hydrophobic filter 70 is installed at a position close to the blood collection bag 20.

The housing 71 is made of, for example, a hard material such as polycarbonate, polyolefin, or hard polyvinyl chloride, and its inner surface is preferably subjected to antithrombotic treatment using heparin or the like. Also,
The filter 72 is a membrane filter having an average pore size of about 0.1 to 1.0 μm, preferably 0.22 to 0.8 μm, and made of a material having hydrophobicity such as polytetrafluoroethylene, polysulfone, and polypropylene. Yes, it allows gas to pass but not blood.

Next, the sealing members 35, 36, 41, 51 and 61 will be described. In addition, in place of each sealing member, a one-touch clamp, a slide clamp, a roller clamp, a Kochel, by using a member or a device that closes the tube from the outside of the tube, such that the flow path can be blocked. Is also good.

Here, since the configuration of each sealing member is almost the same, only the sealing member 41 will be described representatively.
FIG. 4 is a longitudinal sectional view showing a configuration example of the sealing member in the present invention. As shown in the figure, a sealing member 41 is composed of, for example, a short tube 410 made of a flexible resin such as soft polyvinyl chloride, and a short tube 4.
And a cylindrical body 411 whose one end is closed by a solid columnar part 412. In addition, the upstream side 401 of the blood inflow tube 40 connected to the blood collection bag 20 is connected to one end of the short tube 410 in a liquid-tight manner, and the other end of the short tube 410 is connected to the leukocyte removal filter 10. The downstream side 402 of the blood inflow tube 40 is connected in a liquid-tight manner.

Note that one end of the short tube 410 may be directly connected to the blood collection bag 20 without passing through a part of the blood inflow tube 40.
The short tube 410 is connected to a discharge port provided at the upper end of the short tube. Similarly, the sealing member 61 is connected to the gas outflow tube 60.
1 is connected to the blood collection bag 20 through a part of
The blood collection bag 2 does not pass through a part of the gas inflow tube 601 but through an outlet provided at the upper end of the blood collection bag 20.
0 may be directly connected.

The sealing member 35 may be connected to the collection bag 30 through a part of the sixth tube (connecting tube) 31, but may be connected to an outlet provided at the upper end of the collection bag 30. Therefore, it is preferable to connect directly to the collection bag 30. Similarly, the sealing member 36 may be connected to the storage solution bag 36 via a part of the connection tube 31, but may be connected to the storage solution bag 33 via an outlet provided at the upper end of the storage solution bag 33. Is preferably connected directly to Further, the sealing member 51 is connected to the blood outflow tube 5.
Although it may be connected to the branch pipe 52 via a part of 0, it is preferable to be directly connected to the branch pipe 52.

A thin and brittle rupture portion 413 is formed on the outer periphery of the cylindrical body 411, and the solid tube portion 41 together with the short tube 410 from outside the short tube 410 by a finger or the like.
2 is bent to break the broken portion 413, and the solid columnar portion 41 is broken.
By separating 2, the flow path is opened. Cylindrical body 41
Examples of the constituent material 1 include hard polyvinyl chloride,
Hard materials such as polycarbonate and ABS resin can be suitably used. Further, as a constituent material of the short tube 410, for example, a flexible material such as soft polyvinyl chloride can be suitably used.

The tip of the solid column 412 has a wedge shape, and the upper end 414 has a width dimension smaller than the outer diameter of the cylindrical body 411 and smaller than the inner diameter of the first tube 40. It is preferable to adopt a configuration in which the solid columnar portion 412 is large in size and does not block the first tube 40 after the solid columnar portion 412 is broken and separated. Further, as shown in the drawing, a groove 415 for promoting the flow of blood may be provided at the upper end 414 of the solid column 412.

With reference to FIG. 1, the operation of the blood bag 1 with a leukocyte removal filter of the first embodiment (an unnecessary object removing method using the blood bag 1 with a leukocyte removal filter) will be described.
In advance, blood is collected from a donor using the blood collection needle 22 of the blood collection bag 20 by a known method, and for example, 500 mL of blood (whole blood) is stored in the blood collection bag 20, and the blood is collected in the blood collection bag 20 in advance. Contained sterile anticoagulant,
For example, mix well with the CPD solution.

First, the blood collection bag 20 containing whole blood is hung on a stand (not shown), and the collection bag 3
Put 0 on the table. Then, the solid columnar portion 412 of the sealing member 41 is bent to break the break portion 413 to open the flow path. Thereby, the blood in the blood collection bag 20 flows out of the blood collection bag 20 and the blood inflow tube 4
It flows into 0. At this time, the gas in the blood inflow tube 40 and the leukocyte removal filter 10 is flushed downstream by the blood (to the branch pipe 52). This gas is
Since it is sterilized by air existing in the blood inflow tube 40 and the leukocyte removal filter 10 before blood collection, there is no problem in safety.

Next, in the same manner as the sealing member 41, the broken portion of the sealing member 61 is broken to open the flow path. The breaking and opening of the sealing member 61 may be performed simultaneously with the breaking and opening of the sealing member 41. Thus, the blood in the blood collection bag 20 flows into the bypass tube 60, reaches the gas outlet chamber 76 of the hydrophobic filter 70, and stops. On this occasion,
The air in the bypass tube 60 is separated from the branch tube 5 by the blood.
It is washed away to two sides.

The blood that has flowed into the blood inflow tube 40 flows further into the leukocyte removal filter 10 on the downstream side, passes through the filter 12, and the leukocytes and platelets are filtered. At this time, in addition to the air in the blood inflow tube 40 and the leukocyte removal filter 10, the air upstream of the branch tube 52 in the blood outflow tube 50 is the blood pressure because the sealing member 51 is not opened. By T
Through the pipe 52, it is compressed toward the hydrophobic filter 70 side of the bypass tube 60.

Gradually, the filtered blood also flows into the blood outflow tube 50, passes through the T-shaped tube 52, and flows into the bypass tube 60. When a predetermined amount of blood flows into the bypass tube 60, the hydrophobic filter 7
Since a gravitational pressure difference (difference between potential energy and kinetic energy) is generated between the position of the blood in the gas outflow chamber 76 and the blood in the gas outflow chamber 76 of the hydrophobic filter 70, The compressed air pushes back into blood collection bag 20 again. At this time, the bypass tube 6
The air inside 0 is also avoided in the blood collection bag 20.

When the blood flowing into the bypass tube 60 reaches the gas inflow chamber 75 of the hydrophobic filter 70 and stops, the blood in the blood inflow tube 40, the leukocyte removal filter 10, and the blood outflow tube 50 (T-shaped) Tube 5
1) and the air in the bypass tube 60 are all removed by the hydrophobic filter 7 of the bypass tube 60.
0 (the gas outflow chamber 76 side). That is, most of the air is kept inside the blood collection bag 20. The remaining air remains between the blood collection bag 20 of the bypass tube 60 and the hydrophobic filter 70.

Next, the broken portion of the sealing member 51 is broken to open the flow path. Thereby, the blood in the blood collection bag 20 starts flowing out of the blood collection bag 20 again by gravity and flows into the leukocyte removal filter 10. On this occasion,
The blood in the blood collection bag 20 flows again into the bypass tube 60 and the gas outflow chamber 7 of the hydrophobic filter 70.
It reaches 6 and stops. At this time, the air in the bypass tube 60 (upper part than the hydrophobic filter 70) moves below the hydrophobic filter 70, and the blood below the hydrophobic filter 70 in the bypass tube 60 is also reduced by gravity. , Through the T-tube 52 and into the blood outflow tube 50.

After a certain period of time, the blood collection bag 20
The blood inside is completely excreted. Then, the blood collection bag 20
The air inside flows into the blood inflow tube 40 again. Thereby, the blood flowing into the leukocyte removal filter 10 is pushed out into the blood outflow tube 50 and collected in the collection bag 30.

In the case where the leukocyte removal filter 10 after the blood filtration can pass gas,
At this point, blood remains only in the bypass tube 60. On the other hand, when the leukocyte-removing filter 10 after blood filtration has a bubble point higher than the pressure difference due to gravity and cannot pass through the gas, at this point, the inside of the filter 12 of the leukocyte-removing filter 10 or Blood remains in the blood outflow chamber 16, the blood outflow tube 50, and the bypass tube 60.

Next, when pressure is applied to the collection bag 30 by gently grasping the collection bag 30 from the outside, the whole blood from which leukocytes have been removed in the collection bag 20 flows into the blood outflow tube 50. At this time, when the second tube 50 or the first tube 40 on the upstream side of the branch pipe 52 is closed by an instrument (not shown) for closing the tube from the outside such as a clamp, the bypass tube 60 The pressure can be concentrated on the side. As a result, the blood remaining in the blood outflow tube 50 flows into the bypass tube 60, so that the blood in the bypass tube 60 again flows into the gas inflow chamber 7 of the hydrophobic filter 70.
Reach up to 5. At this time, the air in the bypass tube 60 also moves to the gas outflow chamber 76 side (upper side) of the hydrophobic filter 70 again, and the blood above the hydrophobic filter 70 in the bypass tube 60 is removed from the blood collection bag 20. Is discharged.

When the pressure applied to the collection bag 30 is released, the blood in the bypass tube 60 passes through the blood outflow tube 50 and is collected in the collection bag 30.
Thereby, the leukocyte removal filter 10 after the blood filtration is performed.
However, if the gas can pass through, all the blood after the filtration is collected in the collection bag 30 except for a small amount of blood discharged into the blood collection bag 20. On the other hand, even when the leukocyte removal filter 10 after the blood filtration cannot pass gas, the blood remaining in the filter 12 of the leukocyte removal filter 10 and the blood outflow chamber 16 and the trace amount discharged into the blood collection bag 20 Except for the blood, all blood after filtration is collected in the collection bag 30. For this reason, the blood bag 1 with a leukocyte removal filter can recover most of the filtered blood by the above simple operation.

Next, a description will be given of a second embodiment of the blood bag with an unnecessary matter removing filter according to the present invention. FIG. 5 is a circuit diagram showing a second embodiment of the blood bag with an unnecessary matter removing filter according to the present invention. FIGS. 6 and 7 show the second embodiment of the blood bag with an unnecessary matter removing filter according to the present invention. FIG. 9 is a partial configuration diagram showing another embodiment. In addition, description of the common points with the blood bag 1 with an unnecessary matter removal filter of the first embodiment described above will be omitted, and the main differences will be described.

As shown in FIG. 5, in the blood bag 2 with a leukocyte removal filter, which is a blood bag with an unwanted matter removal filter, the third tube (bypass tube) 60
It is extended into a first bag (blood collection bag) 20.
Further, a clamp 62 is provided between the branch pipe 52 of the third tube (bypass tube) 60 and the hydrophobic filter 70. The clamp 62 is preferably a one-touch clamp, a slide clamp, a roller clamp, or the like that can close the tube from the outside of the tube, block its flow path, and open it again.

With this configuration, the blood that has entered the bypass tube 60 can be reliably collected in the blood collection bag 20.
Can be transported (avoided) within. The blood once transferred into the blood collection bag 20 is
There is no backflow inside. In FIG. 5, the third
Tube (bypass tube) 60 is extended to the vicinity of the bottom of the first bag (blood collection bag) 20, but the length of the bypass tube 60 is any length as long as it is extended into the blood collection bag 20. There is no problem even if there is. Other configurations of the blood bag 2 with a leukocyte removal filter are substantially the same as those of the blood bag 1 with a leukocyte removal filter described above.

Here, in the second embodiment shown in FIG. 5, the position of the sealing member 41 is outside the first bag 20, and the connecting portion 23 between the first bag 20 and the first tube 40 is provided.
Near the first bag 20 and the first tube 40
May be provided in the connection part 23 of the above. The position of the sealing member 61 is also outside the first bag 20 and near the connecting portion 24 between the first bag 20 and the third tube 60. May be provided at the connection part 24 of the tube 60. Further, the position of the sealing member 61 may be provided near the tip of the third tube 60 extended into the first bag 20 (see FIG. 6).

The extended third tube 60
May be co-attached to the outer peripheral sealing portion of the first bag 20 at a position facing the connecting portion 24 between the first bag 20 and the third tube 60. In this case, the sealing member 61
An opening hole is provided for the air to flow out by opening the opening.

Further, instead of extending the third tube 60 into the first bag 20, the inside of the first bag 20 is connected to the connecting portion 23 of the first tube 40 and the connecting portion of the third tube 60. It may be partitioned on the 24th side (see FIG. 7). Specifically, a seal portion 25 extending from one end inside the first bag 20 is provided between the connection portion 23 of the first tube 40 and the connection portion 24 of the third tube 60 in the first bag 20. Provided to form a partition. In the embodiment shown in FIG. 7, the seal portion 25 extends along the peripheral portion of the first bag 20 to near the bottom of the first bag 20. Any width, shape, and position may be used as long as the flow of gas discharged into the first bag 20 can be regulated.

With reference to FIG. 5, the operation of the blood bag 2 with a leukocyte removal filter of the second embodiment (a method of removing unnecessary substances using the blood bag 2 with a leukocyte removal filter) will be described.
The operation of the present embodiment is almost the same as that of the blood bag 1 with a leukocyte removal filter of the first embodiment before the operation of the clamp 62, and therefore the description will be focused on the differences.

Blood is collected from a donor using the blood collection needle 22 of the blood collection bag 20 by a known method in advance, and for example, 500 mL of blood (whole blood) is stored in the blood collection bag 20. It is well mixed with a sterilized anticoagulant, for example, a CPD solution, which is contained in advance.

First, the blood collection bag 20 containing whole blood is hung on a stand (not shown), and the collection bag 3
Put 0 on the table. After confirming that the clamp 62 is in the open state, the solid columnar portion 412 of the sealing member 41 is
Is bent to break the break portion 413 to open the flow path. Thereby, the blood in the blood collection bag 20 flows out of the blood collection bag 20 and flows into the blood inflow tube 40. At this time, the gas (air) in the blood inflow tube 40 and the leukocyte removal filter 10 is swept downstream by the blood (toward the branch pipe 52).

Next, in the same manner as the sealing member 41, the break portion of the sealing member 61 is broken to open the flow path. The breaking and opening of the sealing member 61 may be performed simultaneously with the breaking and opening of the sealing member 41. Thereby, a small amount of blood collection bag 20
The blood therein flows into the bypass tube 60 toward the gas outlet chamber 76 of the hydrophobic filter 70.

The blood that has flowed into the blood inflow tube 40 flows further into the leukocyte removal filter 10 on the downstream side, passes through the filter 12, and the leukocytes and platelets are filtered. At this time, in addition to the air in the blood inflow tube 40 and the leukocyte removal filter 10, the air upstream of the T-shaped tube (branch tube) 52 in the blood outflow tube 50 does not have the sealing member 51 opened. Therefore, the blood passes through the T-tube 52 and is compressed toward the hydrophobic filter 70 of the bypass tube 60 by the pressure of the blood.

Gradually, the filtered blood flows into the blood outflow tube 50, passes through the T-shaped tube 52, flows into the bypass tube 60, and when a predetermined amount of blood flows into the bypass tube 60, it becomes hydrophobic. A gravitational pressure difference (difference between potential energy and kinetic energy) is generated between the position of blood in the gas outlet chamber 76 of the filter 70 and the blood flows into the gas outlet chamber 76 of the hydrophobic filter 70. The collected blood is returned to the blood collection bag 20 by the compressed air.
Is pushed back in. At this time, the air in the bypass tube 60 is also avoided in the blood collection bag 20. The blood flowing into the bypass tube 60 is supplied to the hydrophobic filter 7.
It reaches the gas inflow chamber 75 of 0 and stops. At this point, the clamp 62 is closed.

Next, the broken portion of the sealing member 51 is broken to open the flow path. Thereby, the blood in the blood collection bag 20 starts flowing out of the blood collection bag 20 again by gravity and flows into the leukocyte removal filter 10. Since the bypass tube 62 is closed by the clamp 62, the blood in the blood collection bag 20 does not flow into the bypass tube 60. After a certain period of time, all the blood in the blood collection bag 20 is discharged. Then, the air in the blood collection bag 20 flows into the blood inflow tube 40 again. Thereby, the blood flowing into the leukocyte removal filter 10 is pushed out into the blood outflow tube 50 and collected in the collection bag 30. At this point, blood remains in the filter 12 of the leukocyte removal filter 10, the blood outflow chamber 16, the blood outflow tube 50, and the bypass tube 60.

Next, the clamp 62 is opened. Thereby, the blood in the bypass tube 60 passes through the blood outflow tube 50 and is collected in the collection bag 30.
By the above operation, if the leukocyte removal filter 10 after blood filtration is a gas-passable filter, all the blood after the filtration is collected in the collection bag 30. On the other hand, even if the leukocyte removal filter 10 after blood filtration is gas-impermeable, all blood after filtration is recovered except blood remaining in the filter 12 of the leukocyte removal filter 10 and the blood outflow chamber 16. Collected in the bag 30. Therefore, the blood bag 2 with a leukocyte removal filter
Can recover most filtered blood with a simple operation.

Next, a description will be given of a third embodiment of the blood bag with an unnecessary matter removing filter according to the present invention. FIG. 8 is a circuit diagram showing a third embodiment of the blood bag with an unnecessary substance removing filter according to the present invention. FIG. 9 is a circuit diagram showing the third embodiment of the blood bag with an unnecessary substance removing filter according to the present invention. It is sectional drawing which shows the example of a structure of a filter. In addition, description of the common points with the blood bag 1 with an unnecessary matter removal filter of the first embodiment described above will be omitted, and the main differences will be described.

As shown in FIG. 8, in the blood bag 3 with a leukocyte removal filter, which is a blood bag with a filter for removing unwanted matter, the fourth tube 90 is extended into the first bag 20. Further, the clamp 62 is provided on the fourth tube 90. Since these points are the same as those of the blood bag 2 with a leukocyte removal filter of the second embodiment described above, the description is omitted.

The blood bag 3 with a leukocyte removal filter includes a leukocyte removal filter 80 instead of the leukocyte removal filter 10. As shown in FIG. 9, the leukocyte removal filter 80 includes a housing 81 having a blood inlet 83 and a blood outlet 84, similar to the leukocyte removal filter 10, and a blood inflow chamber 85 and a blood outflow chamber 86 in the housing 81. And a gas outlet 88 provided in the housing 81, a blood outlet chamber 86 of the housing 81, and a gas outlet 88 of the housing 81. And a hydrophobic filter 87 provided at the end. Therefore, the leukocyte removal filter 80 is obtained by adding the hydrophobic filter 87 and the gas outlet 88 to the leukocyte removal filter 10. For this reason, the leukocyte removal filter 80 will be described focusing on differences from the leukocyte removal filter 10.

The leukocyte removal filter 80 includes two members in which the periphery of the filter 82 constitutes the housing 81.
The lid having the blood inlet 13 is sandwiched between a vessel having the blood outlet 14 and the vessel having the blood outlet 14, and the lid of the housing and the vessel are joined by ultrasonic fusion or the like.
From the blood inflow chamber 85, always pass through the filter 82, and out of the blood outflow port 84 through the blood outflow chamber 86.
8 does not leak to the gas outlet 87. Further, a hydrophobic filter 88 is provided above the blood outflow chamber 86,
The circumferential portion is fixed by heat fusion, ultrasonic fusion, or the like, and the gas flowing into the blood inflow chamber 85 from the blood inflow port 83 passes through the filter 82 and passes through the blood outflow chamber 83. After passing through the hydrophobic filter 88, the gas flows out (exhausted) from the gas outlet 87, and flows into the blood outflow chamber 86 from the blood outlet 84. It is configured to flow out (exhaust) from the outlet 87.

A first tube (blood inflow tube) 40 is connected to the blood inlet 83 in a liquid-tight manner, and a second tube (blood outflow tube) is connected to the blood outlet 84.
50 is connected in a liquid-tight manner, and a fourth tube (bypass tube) 90 is connected to the gas outlet 87 in a liquid-tight manner. Of the tubes, the inner diameter of the fourth tube 90 is preferably set smaller than the inner diameters of the other tubes. The inner diameter of the fourth tube 90 is the same as that of the third tube 6 used in the first embodiment.
Same as the inner diameter of 0. The length of the fourth tube 90 is 250 to 950 mm, preferably 350 to 750.
mm.

The hydrophobic filter 87 used in this embodiment
As an average pore diameter is about 0.1 to 1.0 μm, preferably 0.22 to 0.8 μm, for example, by using a hydrophobic material such as polytetrafluoroethylene, polysulfone, polypropylene, etc., gas Is configured to pass but not blood. Other configurations of the blood bag 3 with a leukocyte removal filter are almost the same as those of the blood bag 1 with a leukocyte removal filter described above.

With such a configuration, the filtered blood does not flow into the bypass tube 90, so that the air can be easily discharged (avoided) from the collection bag 30 into the blood collection bag 20. . Here, FIG.
In the third embodiment, a leukocyte removal filter 80 in which a hydrophobic filter 87 and a gas outlet 88 are additionally provided is used as the leukocyte removal filter 10, but a hydrophobic filter 70 is provided in the fourth tube (bypass tube) 90.
(See FIG. 3). In this case, it is not necessary to provide the leukocyte removal filter 80 with the hydrophobic filter 87, and the sealing member 62 is provided between the hydrophobic filter 70 and the gas outlet 88. Will be installed.

Further, similarly, in the third embodiment shown in FIG.
Although the leukocyte removal filter 80 provided with an additional gas outlet 88 is used, the fourth tube (bypass tube) 90 may be provided with a hydrophobic filter 70 (see FIG. 3). Although it is not necessary to provide the filter 87, when the blood collection bag 20 has a shape as shown in FIG.
Is installed on the tube 40.

The operation of the blood bag 3 with a leukocyte removal filter of the third embodiment (a blood processing method using the blood bag 3 with a leukocyte removal filter) will be described with reference to FIG. The blood bag 1 with a leukocyte removal filter of the first embodiment
The following description focuses on differences from the leukocyte removal filter 2 of the second embodiment.

Blood is collected in advance from a donor using the blood collection needle 22 of the blood collection bag 20 by a known method, and for example, 500 mL of blood (whole blood) is stored in the blood collection bag 20. Mix well with a sterile anticoagulant, eg, a CPD solution, previously contained within.

First, the blood collection bag 20 containing whole blood is hung on a stand (not shown), and the collection bag 3
Put 0 on the table. After confirming that the clamp 62 is in the open state, the solid columnar portion 412 of the sealing member 41 is
Is bent to break the break portion 413 to open the flow path. Thus, the blood in the blood collection bag 20 flows out of the blood collection bag 20 and flows into the blood inflow tube 40 and the leukocyte removal filter 80.

Then, the white blood cells and platelets are filtered through the filter 82. At this time, since the blood outflow tube 50 is closed by the sealing member 51, the gas (air) in the blood inflow tube 40 and the leukocyte removal filter 10 passes through the hydrophobic filter 87 and flows out to the bypass tube 90. You.

Next, in the same manner as the sealing member 41, the broken portion of the sealing member 61 is broken to open the flow path. Thereby, a small amount of blood in the blood collection bag 20 flows into the bypass tube 90. Since a gravitational pressure difference (difference between potential energy and kinetic energy) occurs between the blood in the blood inflow tube 40 and the blood flowing in the bypass tube 90, the blood in the bypass tube 90 is collected again. Pushed back into bag 20. At this time, the air in the bypass tube 90 is also avoided in the blood collection bag 20. Blood outflow chamber 8 of leukocyte removal filter 80
When 6 is found in the blood, clamp 62 is closed.

Next, the broken portion of the sealing member 51 is broken to open the flow path. Thereby, the blood in the blood collection bag 20 starts flowing out of the blood collection bag 20 again by gravity and flows into the leukocyte removal filter 80. After a certain period of time, all the blood in the blood collection bag 20 is discharged. Then, the air in the blood collection bag 20 flows into the blood inflow tube 40 again. As a result, the blood that has flowed into the leukocyte removal filter 80 is pushed into the blood outflow tube 50 and collected in the collection bag 30. At this point, the leukocyte removal filter 8
Blood remains in the filter 82, the blood outflow chamber 86, and the blood outflow tube 50.

Next, the clamp 62 is opened. Thus, the blood outflow chamber 8 of the leukocyte removal filter 80
6. The blood in the blood outflow tube 50 passes through the blood outflow tube 50 and is collected in the collection bag 30.

By the above operation, if the leukocyte removal filter 80 after blood filtration can pass gas,
All the blood after the completion of the filtration is collected in the collection bag 30.
On the other hand, even if the leukocyte removal filter 80 after blood filtration is a gas impervious filter, the leukocyte removal filter 8
Except for the blood remaining in the zero filter 82, all the blood after the filtration is collected in the collection bag 30. For this reason, the blood bag 3 with a leukocyte removal filter can recover most of the filtered blood by a simple operation.

[0102]

EXAMPLES Next, specific examples of the blood bag with an unnecessary matter removing filter of the present invention will be described.

Example 1 In this example, an experiment was performed using the blood bag 1 with a leukocyte removal filter shown in FIG. 1 and a commercially available blood collection bag. First, a blood bag 1 with a leukocyte removal filter was manufactured using the following members. The storage solution bag 33 was filled with 110 mL of SAGM solution as a red blood cell storage solution, and the blood collection bag 20 was not filled with an anticoagulant.

1. Leukocyte removal filter: Imgard II
1. I-RC (manufactured by Terumo Corporation: leukocyte and platelet removal type) 2. Blood collection bag (first bag): made of soft polyvinyl chloride, 600 mL capacity 3. Collection bag (second bag): made of soft polyvinyl chloride, 550 mL capacity 4. Blood inflow tube (first tube): made of soft polyvinyl chloride, 300 mm 5. Blood outflow tube (second tube): made of soft polyvinyl chloride, 750 mm 6. Bypass tube (third tube): made of soft polyvinyl chloride, 350 mm 7. Blood collection tube (fifth tube): made of soft polyvinyl chloride, 400 mm 8. Connecting tube (sixth tube): made of soft polyvinyl chloride 10. Separation bag: Made of soft polyvinyl chloride, 400 mL capacity Storage liquid bag: Made of soft polyvinyl chloride, 400m
L capacity 11. 11. Hydrophobic filter: made of polytetrafluoroethylene, average pore size 0.65 μm 12. T-shaped tube (branch tube): made of rigid polyvinyl chloride Sealing member: Made of polycarbonate and soft polyvinyl chloride

Next, 500 mL of whole blood was collected from a healthy blood donor using the same commercially available blood collection bag (containing 70 mL of CPD solution) as described in 2 above. This blood collection bag is attached to the blood collection tube 21 of the blood bag 1 with a leukocyte removal filter using a sterile bonding machine 8 (TSCD SC-201A: manufactured by Terumo Corporation / TSCD is a registered trademark of Terumo Corporation). All the collected blood was aseptically joined and transferred to the blood collection bag 20.

After the blood (whole blood) was allowed to stand at 22 ° C. for 6 hours from the blood collection, the blood collection bag 20 of the blood bag 1 with a leukocyte removal filter was hung on a stand (not shown), and The blood processing operation was performed by the blood processing method using the blood bag 1 with the removal filter. The distance (head) from the blood collection bag 20 to the collection bag 30 when the blood collection bag 20 is hung on a stand is 1
It was 10 cm. The time required to filter all blood after opening the sealing member 51 was 8 minutes and 45 seconds.

After all the blood processing operations are completed, the tubes 21, 40, and 60 connected to the blood collection bag 20 and the blood outflow tube 50 connected to the collection bag 30 are sealed at predetermined locations by tube sealing. The blood collection bag 20 and the collection bag 30 were cut off using a machine (Termo Tube Sealer AC-157: manufactured by Terumo Corporation). When the amount of blood remaining in the blood collection bag 20 was confirmed, it was 0.2 mL.

Then, a collection bag 30 containing the blood after filtration (blood from which leukocyte components and platelet components have been removed), a separation bag 32 connected to this bag via a connecting tube 31, and a storage solution Put the bag 33 into a cup of a centrifuge (manufactured by Hitachi Koki Co., Ltd.)
The mixture was centrifuged at 00G for 6 minutes to separate the upper plasma component and the lower red blood cell component. After completion of the centrifugation, the connection bag is taken out of the cup, and the upper layer plasma component is collected from the collection bag 30 to the separation bag 32 using a separation stand (Termo separation stand: Terumo Corporation).
Transported through.

Then, the connection tube 31 was sealed again using the tube sealing machine, and the separation bag 32 was cut off. Next, after removing the collection bag 20 from the separation stand, the erythrocyte preservation solution in the storage solution bag 33 was transferred to the collection bag 30 through the connecting tube 31. Then, using a tube sealing machine, the connection tube 31 was sealed, the collection bag 30 was cut off, and the red blood cell component and the red blood cell preservative were mixed well.

The separation bag 3 obtained by the above operation
With respect to the leukocyte-depleted plasma in No. 2 and the leukocyte-depleted concentrated red blood cells in the collection bag 30, the collection amount and the leukocyte count were measured, and the recovery rate and leukocyte depletion rate were calculated based on the measured values and the values measured in advance. The white blood cell count was measured by the Nageotte method (10-fold dilution). The results are shown in Tables 1 and 2.

Example 2 In this example, an experiment was performed under the same conditions as in Example 1 above, using a blood bag 2 with a leukocyte removal filter shown in FIG. 5 and a commercially available blood collection bag. . First, a blood bag 2 with a leukocyte removal filter was prepared. Except for the length of the bypass tube 60 and the use of the clamp 62, the blood bag 1 is the same as the blood bag 1 with a leukocyte removal filter of Example 1, and therefore only the differences will be described.

1. 1. Bypass tube (third tube): made of soft polyvinyl chloride, 410 mm Cleanme: One Touch Cleanme

Next, in the same manner as in Example 1, 500 mL of whole blood was collected from a healthy blood donor using a commercially available blood collection bag (containing 70 mL of CPD solution). This blood collection bag is connected to a sterile bonding machine 8 (TSCD SC-201A: Terumo Corporation).
/ TSCD is a registered trademark of Terumo Corporation. ) Was aseptically joined to the blood collection tube 21 of the blood bag 2 with a leukocyte removal filter, and all the collected blood was transferred to the blood collection bag 20.

After this blood (whole blood) was allowed to stand at 22 ° C. for 6 hours after blood collection, the blood collection bag 20 of the blood bag 2 with a leukocyte removal filter was hung on a stand (not shown), and The blood processing operation was performed by the blood processing method using the blood bag 2 with the removal filter. The distance (head) from the blood collection bag 20 to the collection bag 30 when the blood collection bag 20 was hung on a stand was 110 cm, which was the same as in Example 1. Also, the sealing member 51
The time required to filter all blood after opening was 8 minutes and 12 seconds.

After all the blood processing operations are completed, the same blood component separation operation as in Example 1 is performed, and the separation bag 32
The leukocyte-depleted plasma and the leukocyte-depleted concentrated red blood cells in the collection bag 30 were obtained therein. For these blood components,
The leukocyte count was measured, and the recovery rate and leukocyte removal rate were calculated based on the measured values and the values measured in advance. The white blood cell count was measured by the Nageotte method (10-fold dilution). The results are shown in Tables 1 and 2.

Example 3 In this example, an experiment was performed under the same conditions as in Example 1 above, using a blood bag 3 with a leukocyte removal filter shown in FIG. 8 and a commercially available blood collection bag. . First, a blood bag 3 with a leukocyte removal filter was prepared. Note that the blood bag 1 is the same as the blood bag 1 with a leukocyte removal filter of Example 1 except that the leukocyte removal filter 80, the bypass tube 90, and the cleanser 62 are not used, and the hydrophobic filter 70 is not used. .

[0117] 1. 1. Leukocyte removal filter: Leukocyte and platelet removal type, with hydrophobic filter 2. Bypass tube (fourth tube): made of soft polyvinyl chloride, 350 mm Cleanme: One Touch Cleanme

Next, in the same manner as in Example 1, 500 mL of whole blood was collected from a healthy blood donor using a commercially available blood collection bag (containing 70 mL of CPD solution). This blood collection bag is connected to a sterile bonding machine 8 (TSCD SC-201A: Terumo Corporation).
/ TSCD is a registered trademark of Terumo Corporation. ) Was aseptically joined to the blood collection tube 21 of the blood bag 3 with the leukocyte removal filter, and all the collected blood was transferred to the blood collection bag 20.

After the blood (whole blood) was allowed to stand at 22 ° C. for 6 hours after blood collection, the blood collection bag 20 of the blood bag 3 with a leukocyte removal filter was hung on a stand (not shown), and The blood processing operation was performed by the blood processing method using the blood bag 3 with the removal filter. The distance (head) from the blood collection bag 20 to the collection bag 30 when the blood collection bag 20 was hung on a stand was 110 cm, which was the same as in Example 1. Also, the sealing member 51
It took 9 minutes and 6 seconds to filter all of the blood after opening.

After all the blood processing operations are completed, the same blood component separation operation as in Example 1 is performed, and the separation bag 32
The leukocyte-depleted plasma and the leukocyte-depleted concentrated red blood cells in the collection bag 30 were obtained therein. For these blood components,
The leukocyte count was measured, and the recovery rate and leukocyte removal rate were calculated based on the measured values and the values measured in advance. The white blood cell count was measured by the Nageotte method (10-fold dilution). The results are shown in Tables 1 and 2.

(Comparative Example) In this example, an experiment was conducted under the same conditions as in Example 1 above, using a blood bag 100 with a leukocyte removal filter shown in FIG. 10 and a commercially available blood collection bag.

Here, the leukocyte removal filter 100 will be described with reference to FIG. 10. A blood bag 100 with a leukocyte removal filter is a blood collection bag 120 provided with a blood collection needle 122 for collecting blood from a donor. A leukocyte removal filter 110 for removing leukocytes,
Collection bag 1 for storing blood from which leukocytes have been removed
30, a separation bag 132 for storing blood components and a storage solution bag 133 are connected by tubes 131, 140 and 150, and further upstream of the leukocyte removal filter 110 so as to bypass the leukocyte removal filter 110. Side tube 140 and downstream tube 1
It has a bypass tube 160 connecting the 50.
The bypass tube 160 is provided with a hydrophobic filter 170.

The blood processing method for the blood bag 100 with the leukocyte removal filter is as follows.
1 is opened, the blood in the blood collection bag 120 is passed through the leukocyte removal filter 110 via the upstream tube 140 to remove leukocytes, and the blood from which leukocytes have been removed is collected via the downstream tube 150 into the collection bag 130. To collect.
Next, the air in the tubes 140 and 150 and the leukocyte removal filter 110 which flowed together with the blood from which leukocytes have been removed into the collection bag 130 is removed by the bypass tube 160
Is used to transfer the blood to the blood collection bag 120.

First, a blood bag 100 with a leukocyte removal filter was prepared. Note that the bypass tube 160 is used and the bypass tube 60 is not used. Except for using the clamps 151 and 161 and not using the sealing members 51 and 61, the same members as those in the blood bag 1 with a leukocyte removal filter of Example 1 were used, so only the differences will be described.

[0125] 1. 1. Bypass tube: Made of soft polyvinyl chloride, 200 mm Cleanme: One Touch Cleanme

Next, as in Example 1, 500 mL of whole blood was collected from a healthy blood donor using a commercially available blood collection bag (containing 70 mL of CPD solution). This blood collection bag is connected to a sterile bonding machine 8 (TSCD SC-201A: Terumo Corporation).
/ TSCD is a registered trademark of Terumo Corporation. ) Was aseptically joined to the blood collection tube 21 of the blood bag 3 with the leukocyte removal filter, and all the collected blood was transferred to the blood collection bag 20.

After this blood (whole blood) was allowed to stand at 22 ° C. for 6 hours after blood collection, the blood collection bag 20 of the blood bag 100 with a leukocyte removal filter was set on a stand (not shown).
And a blood processing operation was performed by the blood processing method of the blood bag 100 with a leukocyte removal filter described above.
The distance (head) from the blood collection bag 120 to the collection bag 130 when the blood collection bag 120 was hung on a stand was 110 cm, which was the same as in Example 1. The time required to filter all the blood after opening the sealing member 141 was 9 minutes and 0 seconds.

After all the blood in the blood collection bag 120 is filtered, before the air in the leukocyte removal filter 110 and the air in the downstream tube 150 are transferred into the blood collection bag 120, the filtered blood flows into the leukocyte removal filter 110. The clamp 151 was closed to prevent backflow.
Then, the clamp 161 is opened, a large amount of air in the collection bag 130 is collected above the collection bag 130, and the collection bag 130 is strongly grasped from outside the collection bag 130 to transfer the air into the bypass tube 160. However, when the blood remaining in the downstream tube 150 reached the hydrophobic filter 170, the hydrophobic filter 170 was blocked, so that air could not be transferred.

Therefore, the clamp 161 is once closed, and the blood remaining in the downstream tube 150 (blood outflow tube) is squeezed out into the collection bag 130 using roller pliers (manufactured by Terumo Corporation). , Collection bag 1
The air collected above 30 was replaced.

Thereafter, the clamp 161 is opened again,
The air was transferred into the bypass tube 160 by squeezing the collection bag 130 from the outside of the collection bag 130. At this time, blood (blood before filtration) remaining in the upstream tube 140 (blood inflow tube) becomes a strong resistance,
Until this blood is transferred into the blood collection bag 120,
It was necessary to grasp the collection bag 130 with considerably strong force.

[0131] The air in the collection bag 130 is
After being transferred into 20, the clamp 161 was closed. Next, the clamp 151 was opened, and the blood in the blood collection bag 120 was passed through the leukocyte removal filter 110 and flowed into the collection bag 130. At this time, the leukocyte removal filter 1
The blood after filtration remains in the filter, the blood outflow chamber, and the downstream tube 150 of the collection bag 1.
30 could not be recovered.

After all the blood processing operations are completed, the same blood component separation operation as in the first embodiment is performed.
In 2, leukocyte-depleted plasma and leukocyte-depleted concentrated red blood cells in the collection bag 130 were obtained. For these blood components, the collected amount, the white blood cell count was measured, and the
The recovery rate and leukocyte removal rate were calculated. The white blood cell count was measured by the Nageotte method (10-fold dilution). The results are shown in Tables 1 and 2.

[0133]

[Table 1]

[0134]

[Table 2]

(Experimental Results) From the above experiments, the experimental results shown in Tables 1 to 3 were obtained. It should be noted that there was no significant difference in the time required for filtering whole blood between Examples 1 to 3 and the comparative example, and it was confirmed that the experiment was performed under the same conditions. In the leukocyte-depleted plasma shown in Table 1, no remarkable difference was observed between Examples 1 to 3 and Comparative Example.
It was confirmed that in Examples 1 to 3, the leukocyte-reduced concentrated erythrocytes shown in (1) and (2) had a better erythrocyte collection rate than the comparative example. Further, in the comparative example, when the air in the collection bag 130 is avoided in the blood collection bag 120, it is necessary to collect the blood in the blood outflow tube 150 into the collection bag 130 using roller pliers. Processing took time.

[0136]

As described above, according to the blood bag with an unwanted matter removing filter of the present invention, the bypass tube has one end communicating with the blood outflow tube and the other end communicating with the blood collection bag. A hydrophobic filter that allows gas to pass through but does not allow blood to pass is provided in the middle of the process, so it is possible to avoid air in the waste removal filter, each tube, and the collection bag into the blood collection bag with a simple operation. it can. In addition, blood from which unnecessary substances have been removed can be collected at a high yield, and can be stored for a long period of time.

Further, according to the blood bag with an unnecessary matter removing filter of the present invention, one end communicates with the blood outflow side of the unnecessary matter removing filter, and the other end has a bypass tube which communicates with the blood collection bag. A hydrophobic filter that allows gas to pass but does not allow blood to pass may be provided on the way, so it is possible to avoid air in the unnecessary filter, each tube, and the collection bag into the blood collection bag with a simple operation. it can. In addition, blood from which unnecessary substances have been removed can be collected at a high yield, and can be stored for a long period of time.

Further, according to the blood bag with an unnecessary matter removing filter of the present invention, one end communicates with the blood outflow side of the unnecessary matter removing filter via the hydrophobic filter, and the other end has the first kind.
A bypass tube communicating with the bag may be provided, so that the air in the waste removal filter, in each tube and in the collection bag can be avoided in the blood collection bag by a simple operation. In addition, blood from which unnecessary substances have been removed can be collected at a high yield, and can be stored for a long period of time.

[0139]

[Brief description of the drawings]

FIG. 1 is a circuit diagram showing a first embodiment of a blood bag with an unnecessary matter removing filter according to the present invention.

FIG. 2 is a longitudinal sectional view showing a configuration example of an unnecessary substance removing filter according to the present invention.

FIG. 3 is a longitudinal sectional view showing a configuration example of a hydrophobic filter in the present invention.

FIG. 4 is a longitudinal sectional view showing a configuration example of a sealing member in the present invention.

FIG. 5 is a circuit diagram showing a second embodiment of the blood bag with an unnecessary matter removing filter according to the present invention.

FIG. 6 is a partial configuration diagram showing another embodiment of the blood bag with an unnecessary matter removing filter according to the second embodiment of the present invention.

FIG. 7 is a partial structural view showing another embodiment of the second embodiment of the blood bag with an unnecessary matter removing filter of the present invention.

FIG. 8 is a circuit diagram showing a third embodiment of the blood bag with an unnecessary matter removing filter according to the present invention.

FIG. 9 is a cross-sectional view showing a configuration example of a waste filter in a third embodiment of the blood bag with a waste removal filter according to the present invention.

FIG. 10 is a circuit diagram showing a conventional example of a blood bag with a leukocyte removal filter of the present invention.

[Explanation of symbols]

 1,2,3 Unnecessary matter removal filter blood bag 10,80 Unnecessary matter removal filter 20 First bag 22 Blood collection needle 30 Second bag 32 Separation bag 33 Storage liquid bag 40 First tube 50 Second tube 60 Third tube 70 Hydrophobic filter 90 Fourth tube

Claims (14)

[Claims] 1. A first bag for storing blood containing unnecessary matter, a housing, and a filter provided to divide the inside of the housing into a blood inflow chamber and a blood outflow chamber for separating unnecessary matter. Waste filter consisting of
A first tube having one end communicating with the first bag and the other end communicating with the blood inflow side in the housing, a second bag for storing blood from which unnecessary matter is separated, A blood bag with an unwanted matter removal filter, comprising: a second tube communicating with the second bag and the other end communicating with a blood outflow side in the housing, one end of which is a blood outflow chamber in the housing or A third tube communicating with the second tube and having the other end communicating with the first bag is provided. A hydrophobic filter that allows gas but does not allow blood is provided in the middle of the third tube. A blood bag with a filter for removing unwanted matter, wherein the blood bag is provided. 2. The blood bag with an unnecessary matter removing filter according to claim 1, wherein the unnecessary matter is at least one of white blood cells, platelets, microaggregates, viruses, bacteria, prions, and pathogenic substances. 3. The hydrophobic filter comprises a housing, and a filter provided so as to divide the inside of the housing into a blood inflow chamber and a blood outflow chamber, but allows gas to pass therethrough but does not allow blood to pass therethrough. The blood bag with an unnecessary matter removing filter according to claim 1, which is arranged in a vertical direction. 4. The blood bag with an unnecessary matter removing filter according to claim 1, wherein the third tube extends into the first bag. 5. The filter according to claim 1, wherein the inside of the first bag is partitioned into a connecting portion of the first tube and a connecting portion of the third tube. With blood bag. 6. The sealing member according to claim 1, wherein a sealing member is provided near a connection portion of the first tube, the third tube, and the second tube with the third tube. Blood bag with filter for removing unwanted matter. 7. A first bag for accommodating blood containing unnecessary matter, a housing, and a housing provided to divide the inside of the housing into a blood inflow chamber and a blood outflow chamber to separate the unnecessary matter. A filter provided to divide a blood outflow chamber of the housing from a gas outlet of the housing, the filter including a hydrophobic filter that allows gas to pass therethrough but does not allow blood to pass therethrough; A first tube communicating with the bag of the other end and the other end communicating with the blood inflow side in the housing, and a second tube for storing the blood from which unnecessary matter has been separated.
A second tube having one end communicating with the second bag, the other end communicating with the blood outlet side in the housing, one end communicating with the gas outlet, and the other end being connected to the first bag. And a fourth tube communicating with the bag. 8. The blood bag with an unnecessary matter removing filter according to claim 7, wherein the unnecessary matter is at least one of white blood cells, platelets, microaggregates, viruses, bacteria, prions, and pathogenic substances. 9. The blood bag with an unnecessary matter removing filter according to claim 7, wherein the hydrophobic filter of the unnecessary matter removing filter is provided above a blood outflow chamber of the housing. 10. The blood bag with an unwanted matter removing filter according to claim 7, wherein the fourth tube is extended into the first bag. 11. The unnecessary part according to claim 7, wherein the inside of the first bag is partitioned into a connecting portion side of the first tube and a connecting portion side of the third tube. Blood bag with object removal filter. 12. A sealing member is provided near a communication portion of the first tube, the fourth tube, and the second tube with the fourth tube. A blood bag with an unnecessary matter removing filter according to item 1. 13. A first tube having a blood collection needle at a tip thereof connected to the first bag.
3. The blood bag with an unnecessary substance removing filter according to any one of 2. 14. The blood bag with an unnecessary matter removing filter according to claim 1, wherein a separation bag for storing a blood component is connected to the second bag via a sixth tube. .