JP2009101187A - Blood component sampling circuit - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a blood component sampling circuit for filtering out a predetermined cell in a predetermined blood component with high precision without elongating a working time. <P>SOLUTION: The blood component sampling circuit 1 is provided with a leucocyte filter unit 40 which is formed by integrating a reserver 42 capable of storing platelet-rich plasma centrifugally separated by a centrifugal separator 20 by a single sampling cycle; with a leucocyte filter 41 filtering out the leucocyte. The platelet-rich plasma separated by the centrifugal separator 20 is temporarily stored in the reserver 42 for each sampling cycle and the platelet-rich plasma is guided to the leucocyte filter 41 to filter out the leucocyte. <P>COPYRIGHT: (C)2009,JPO&INPIT

Description

  The present invention relates to a blood component collection circuit used in blood component blood collection. More specifically, the present invention relates to a blood component collection circuit that can efficiently collect high-quality blood components.

  At the time of blood collection, for the purpose of effective use of blood and reduction of burden on blood donors, the blood sample is separated into each blood component by centrifugation, etc., and only the components necessary for the transfuser are collected. Ingredients are collected to return the ingredients to the blood donor.

  In such component blood collection, when obtaining a platelet preparation, it is necessary to remove blood components (white blood cells) that are not desired to be collected and that are mixed in the collected platelet concentrate. This is to prevent fever, alloantigen sensitization, or viral infection. Thus, in order to remove leukocytes mixed in the platelet concentrate, a blood component collection circuit incorporating a leukocyte removal filter is used.

  An example of such a blood component collection circuit is described in Japanese Patent Application Laid-Open No. 2002-218772. As shown in FIG. 9, the blood component blood collection circuit disclosed herein includes a centrifuge 520 that separates blood into a plurality of blood components, a first line 521 that introduces blood into the centrifuge 520, A second line 522 that discharges blood components from the centrifuge 520, a temporary storage bag 527 that is connected to the second line 522 and temporarily stores concentrated platelet plasma, and separates white blood cells from the concentrated platelet plasma A leukocyte removal filter 561 to be removed and a platelet collection bag 526 for storing the concentrated platelet plasma after passing through the leukocyte removal filter 561 are provided.

  In this blood component collection circuit, the concentrated platelet plasma collected in a plurality of cycles is temporarily stored in the temporary storage bag 527, and the concentrated platelet plasma is filtered by the leukocyte removal filter 561 when the blood is returned in the final cycle. Concentrated platelet plasma after filtration is stored.

JP 2002-218772 A

  However, in the blood component collection circuit disclosed in the above-mentioned Japanese Patent Application Laid-Open No. 2002-218772, filtration by the leukocyte removal filter 561 is performed only once, and 200 to 250 mL of concentrated platelets is limited when returning blood in the final cycle If it is not done in time, the work time will be long. For this reason, in general, the working time is prevented from becoming long by providing a large head or increasing the filtration rate using a pump. However, the performance of the leukocyte removal filter is generally dependent on the flow rate, and there is a problem that leukocytes are more likely to leak out when the filtration rate is high than when the filtration rate is low.

  Accordingly, the present invention has been made to solve the above-described problems, and provides a blood component collection circuit capable of removing leukocytes in concentrated platelet plasma with high accuracy without extending the working time. The task is to do.

  A blood component collection circuit according to the present invention made to solve the above problems is a blood component collection circuit that separates blood into a plurality of blood components and collects the separated blood components. A centrifuge for separating blood components; a first line for flowing blood into an inlet of the centrifuge; a second line connected to an outlet of the centrifuge; and the second line A blood component reservoir that temporarily stores concentrated platelet plasma of blood components separated by the centrifuge, and a blood component reservoir that is connected to the blood component reservoir and separates and removes white blood cells from the concentrated platelet plasma And a blood component collection bag that is connected to the cell separation filter and stores the concentrated platelet plasma after passing through the cell separation filter. As a connection passage between the reservoir and the cell separation filter, an inflow passage through which the concentrated platelet plasma flows into the cell separation filter and a discharge passage through which air from the cell separation filter is discharged are provided independently. It is characterized by.

In this blood component collection circuit, concentrated platelet plasma among the blood components separated by the centrifuge is temporarily stored in the blood component storage unit for each cycle. The concentrated platelet plasma stored in the blood component storage unit passes through the cell separation filter and is collected in a blood component collection bag for each cycle. In other words, in this blood component collection circuit, the concentrated platelet plasma is filtered by the cell separation filter every cycle.
Since the blood component reservoir and the cell separation filter are independently connected to each other, an inflow path for allowing concentrated platelet plasma to flow into the cell separation filter and an exhaust path for discharging air from the cell separation filter are provided. In each cycle, the concentrated platelet plasma stored in the blood component reservoir can be reliably introduced into the cell separation filter and filtered.
Therefore, the predetermined blood component stored in the blood component storage section may be filtered through the cell separation filter before returning blood in the current cycle and collecting concentrated platelet plasma in the next cycle. For this reason, the flow rate of the concentrated platelet plasma when passing through the cell separation filter can be lowered, and the cell removal performance by the cell separation filter can be improved. Moreover, the work time does not increase. Therefore, leukocytes in the concentrated platelet plasma can be removed with high accuracy without extending the working time.

In the blood component collection circuit according to the present invention, the inlet of the blood component reservoir in the second line is provided above the liquid level of the predetermined blood component stored in the blood component reservoir. It is desirable that
This is because it is possible to reliably prevent the predetermined blood component stored in the blood component storage unit from flowing backward.

In the blood component collection circuit according to the present invention, it is desirable that the volume of the blood component reservoir is variable.
By doing so, the air in the blood component collection circuit can also be temporarily stored in the blood component storage part, so that it is not necessary to provide a separate filter or airbag as described above. Moreover, a completely closed circuit configuration can be obtained.

In the blood component collection circuit according to the present invention, the discharge port in the blood component storage part of the discharge path is provided above the liquid level of the predetermined blood component stored in the blood component storage part. It is desirable that
This is because when the predetermined blood component is introduced into the cell separation filter, the air of the cell separation filter can be surely discharged.

  A blood component collection device according to the present invention includes any one of the blood component collection circuits described above and a clamp provided in the blood component collection circuit, and separates blood into a plurality of blood components by the centrifuge. A blood component collection device that collects concentrated platelet plasma from the separated blood components and returns the red blood cells for a plurality of cycles, wherein the clamp is switched and separated by the centrifuge for each cycle. The concentrated platelet plasma is stored in the blood component storage unit, and the stored concentrated platelet plasma is filtered through each cell separation filter and collected in the blood component collection bag for each cycle. To do.

  Since this blood component collection device includes the above-described blood component collection circuit, leukocytes in the concentrated platelet plasma can be removed with high accuracy without extending the working time.

  In addition, the filter unit according to the present invention includes a blood reservoir that temporarily stores concentrated platelet plasma and a cell separation filter that is connected to the blood reservoir and separates and removes predetermined cells from the blood. A filter unit, an inflow port through which the concentrated platelet plasma flows into the blood reservoir, an inflow path through which the concentrated platelet plasma flows from the blood reservoir into the cell separation filter, and the cell separation filter It comprises an outflow port through which concentrated platelet plasma flows out, and a discharge path through which air is discharged from the cell separation filter to the blood reservoir.

  In this filter unit, an inflow path for allowing blood to flow from the blood storage section to the cell separation filter and a discharge path for discharging air from the cell separation filter to the blood storage section are provided separately, so that the air is stored in the blood storage section. The concentrated platelet plasma can be reliably introduced into the cell separation filter and filtered.

In the filter unit described above, it is desirable that the discharge port in the blood storage part of the discharge path is provided above the liquid level of the concentrated platelet plasma stored in the blood storage part.
Thereby, when blood is introduced into the cell separation filter, the air of the cell separation filter can be surely discharged.

Moreover, in each filter unit described above, it is desirable that the inflow port of the blood reservoir in the inflow port is provided above the liquid level of the concentrated platelet plasma stored in the blood reservoir.
This is because it is possible to reliably prevent the concentrated platelet plasma stored in the blood storage part from flowing backward.

  According to the blood component collection circuit according to the present invention, as described above, leukocytes in the concentrated platelet plasma can be removed with high accuracy without extending the working time.

It is a circuit block diagram which shows the blood component collection circuit which concerns on embodiment. It is a front view which shows the cell removal filter unit which concerns on embodiment. It is a side view which shows the cell removal filter unit which concerns on embodiment. It is sectional drawing which shows the cell removal filter unit which concerns on embodiment. It is a front view which shows the cell removal filter unit which concerns on another form. It is a front view which shows the cell removal filter unit which concerns on another form. It is a front view which shows the cell removal filter unit which concerns on another form. It is a front view which shows the cell removal filter unit which concerns on another form. It is a circuit block diagram which shows the conventional blood component collection circuit.

  Hereinafter, a most preferred embodiment in which the blood component collection circuit of the present invention is embodied will be described in detail with reference to the drawings. The blood component collection circuit according to the present embodiment is suitable for being attached to a device for collecting a platelet preparation. Therefore, FIG. 1 shows the configuration of the blood component collection circuit according to the present embodiment. FIG. 1 is a circuit configuration diagram of a blood component collection circuit 1.

  The blood component collection circuit 1 includes a centrifuge 20 for centrifuging blood, a first line 21 connected to the inlet 143 of the centrifuge 20, and a first line 21 connected to the outlet 144 of the centrifuge 20. 2, a third line 23 connected to the first line 21, a plasma collection bag 25, a platelet collection bag 26, an air bag 27, and a leukocyte removal filter unit 40.

  The first line 21 includes a blood collection needle side first line 21 a to which the blood collection needle 30 is connected, and a centrifuge side first line 21 b connected to the inlet 143 of the centrifuge 20. The blood collecting means is not limited to the blood collecting needle 30 and may be, for example, a connecting portion (for example, a metal or synthetic resin needle) for connecting to a blood pool such as a blood bag. As this blood collection needle 30, for example, a known metal needle may be used.

  The blood collection needle side first line 21a is formed by connecting a plurality of soft resin tubes. The blood collection needle side first line 21a includes a branch connector 21c for connection with the third line 23 and a chamber 21d for removing bubbles and microaggregates from the blood collection needle 30 side. The other end of the blood collection needle side first line 21a is connected to the centrifuge side first line 21b connected to the first tube 25a of the plasma collection bag 25 and the inlet 143 of the centrifuge 20. It is connected to the connector 21f. The chamber 21d is connected to a filter 21i that is air permeable and impermeable to bacteria. Further, a clamp is attached between the blood collection needle 30 and the branch connector 21c of the blood collection needle side first line 21a.

  The second line 22 is a branch connector for connecting to the tube including the second tube 25b of the plasma collection bag 25, the third tube 26a of the leukocyte removal filter unit 40, and the bubble removal filter 22f from the centrifuge 20 side. 22a.

  One end of the third line 23 is connected to a connection branch connector 21 c provided on the first line 21. The third line 23 includes a foreign substance removing filter 23b, a bubble removing chamber 23c, and an anticoagulant container connecting needle 23d from the branch connector 21c side.

  The plasma collection bag 25 is a container for collecting (storing) plasma. The plasma collection bag 25 includes a first tube 25a and a second tube 25b. The first tube 25a is connected to the branch connector 22a. On the other hand, the second tube 25 b is connected to the branch connector 21 f of the first line 21. Thus, a plasma collection branch line for collecting plasma is constituted by the plasma collection bag 25 and the second tube 25b. A clamp is attached to each of the first tube 25a and the second tube 25b.

  The platelet collection bag 26 is a container for collecting (storing) plasma (predetermined blood component) containing platelets after passing through a leukocyte removal filter unit 40 described later. The platelet collection bag 26 includes a fourth tube 26b connected to the leukocyte removal filter unit 40, and an air bag 27 for storing the air in the bag via the tube 26c.

  In the following description, plasma containing platelets (predetermined blood component) is referred to as “rich platelet plasma”, and the concentrated platelet plasma collected (stored) in the platelet collection bag 26 is referred to as “platelet preparation”. .

  A leukocyte removal filter unit 40 for separating and removing leukocytes (predetermined cells) from the concentrated platelet plasma is installed between the third tube 26a and the fourth tube 26b. That is, the third tube 26a, the leukocyte removal filter unit 40, the fourth tube 26b, and the platelet collection bag 26 constitute a branch line for collecting platelets for collecting platelets (platelet preparation). A clamp is attached to each of the fourth tube 26 b that connects the platelet collection bag 26 and the leukocyte removal filter unit 40 and the tube 26 c that connects the platelet collection bag 26 and the air bag 27.

  Here, the cell removal filter unit of the present invention will be described with reference to FIGS. The cell removal filter unit according to the present embodiment is a leukocyte removal filter unit that removes leukocytes from concentrated platelet plasma. FIG. 2 is a front view showing the leukocyte removal filter unit. FIG. 3 is a side view showing the leukocyte removal filter unit. FIG. 4 is a cross-sectional view showing the leukocyte removal filter unit.

  As shown in FIGS. 2 and 3, the leukocyte removal filter unit 40 includes a leukocyte removal filter 41 that removes leukocytes from the concentrated platelet plasma, a reservoir 42 that temporarily stores concentrated platelet plasma for one cycle, blood, And an air vent filter 43 for taking air in and out of the component collecting circuit 1. A reservoir 42 is disposed above the leukocyte removal filter 41, and an air vent filter 43 is disposed above the reservoir 42.

  As described above, the reservoir 42 is disposed above the leukocyte removal filter 41, and the reservoir 42 can temporarily store the concentrated platelet plasma for one cycle. Can be removed. Further, since the leukocyte removal filter 41 and the reservoir 42 are integrated to form the leukocyte removal filter unit 40, the configuration of the blood component collection circuit 1 is not complicated. Therefore, the work load does not increase in the circuit mounting work to the apparatus.

  Here, the air vent filter 43 is for taking in and out the air in the blood component collection circuit 1. Therefore, when air is taken into the blood component collection circuit 1, it is necessary to take in clean air, so the air vent filter 43 is a breathable and bacteria-impermeable filter. Further, the air vent filter is provided to allow the air in the blood component collecting circuit 1 to be taken in and out so that the pressure fluctuation in the circuit 1 does not occur.

  A discharge port 44 for discharging the concentrated platelet plasma after removing the leukocytes is provided below the leukocyte removal filter 41, and an inflow port 45 for allowing the concentrated platelet plasma before removing the leukocytes to flow in is provided below the reservoir 42. ing. An opening (inlet) 45 a of the inflow port 45 is provided so as to be positioned above the liquid level of the concentrated platelet plasma stored in the reservoir 42. This is to prevent the concentrated platelet plasma stored in the reservoir 42 from flowing backward.

Further, as shown in FIG. 4, a communication path 46 is formed for communicating the reservoir 42 and the leukocyte removal filter 41. The cross-sectional area (opening area) of the communication path 46 is ensured to be 0.7 cm ² or more. Thereby, even if the inlet side of the leukocyte removal filter 41 is replaced with air every cycle, the concentrated platelet plasma stored in the reservoir 42 can be reliably introduced into the leukocyte removal filter 41 and filtered. Yes.
The leukocyte removal filter 41 is formed by laminating one or more porous bodies such as a woven fabric, a nonwoven fabric, a mesh, and a foam made of a synthetic resin such as polypropylene, polyester, polyurethane, and polyamide in the casing 41a. The filtration member is inserted and configured.

  Here, a modified example of the leukocyte removal filter unit will be described. First, as in the present embodiment, a leukocyte removal filter unit of the type that is performed in a common passage for inflow of concentrated platelet plasma and discharge of air will be described with reference to FIGS. This type is provided with a container for taking in and out the air in the blood component collection circuit 1, and the other components are the same as the above-described leukocyte removal filter unit 40, and therefore are denoted by the same reference numerals. The description is omitted.

  The leukocyte removal filter unit 40a shown in FIG. 5 includes a leukocyte removal filter 41 that removes leukocytes from the concentrated platelet plasma, a reservoir 42 that temporarily stores one cycle of concentrated platelet plasma, and a blood component collection circuit 1 And an air bag 47 for taking air in and out. The airbag 47 is connected to the reservoir 42 by a tube 47. Thereby, since the air in the blood component collection circuit 1 enters and exits the airbag 47, it does not come into contact with the outside air. That is, by using the leukocyte removal filter unit 40a, a completely closed circuit configuration can be obtained.

  A leukocyte removal filter unit 40b shown in FIG. 6 has a leukocyte removal filter 41 that removes leukocytes from the concentrated platelet plasma, and a reservoir 42 that temporarily stores the concentrated platelet plasma for one cycle. The upper part 42a of the reservoir 42 is a variable container. Thereby, the processing of the air in the blood component collection circuit 1 can be performed in the reservoir 42. Therefore, even when the leukocyte removal filter unit 40b is used, a completely closed circuit configuration can be obtained.

  Next, a leukocyte removal filter unit of a type that performs the inflow of concentrated platelet plasma and the discharge of air in independent passages, unlike the one in the present embodiment, will be described with reference to FIG. 7 and FIG. The leukocyte removal filter unit 50 shown in FIG. 7 has a leukocyte removal filter 51 that removes leukocytes from concentrated platelet plasma, and a bag (reservoir) 52 that temporarily stores concentrated platelet plasma for one cycle. .

  Below the bag 52, an inflow port 55 for allowing the concentrated platelet plasma before leukocyte removal to flow in, an introduction port 56 for introducing the concentrated platelet plasma stored in the bag 52 into the leukocyte removal filter 51, and leukocytes An exhaust port 57 for discharging the air in the removal filter 51 into the bag 52 is provided. These three ports 55, 56, 57 communicate with respective spaces 52 a, 52 b, 52 c divided into two by partition walls 58, 59 provided in the bag 52. That is, the inflow port 55 communicates with the space 52a, the introduction port 56 communicates with the space 52b, and the exhaust port 57 communicates with the space 52c. Thereby, both the opening (inlet) 55a of the inflow port 55 and the opening (exhaust) 57a of the exhaust port 57 are provided above the liquid level of the concentrated platelet plasma stored in the bag 52. . For this reason, the concentrated platelet plasma stored in the bag 52 can be prevented from flowing back, and the air can be discharged from the leukocyte removal filter 51 with certainty.

  Further, the other ends of the introduction port 56 and the exhaust port 57 communicate with each other above the leukocyte removal filter 51. Note that the openings of the introduction port 56 and the exhaust port 57 in the leukocyte removal filter 51 are disposed at a position where the introduction port 56 is lower than the exhaust port 57. In FIG. 7, the diameters of the introduction port 56 and the exhaust port 57 are the same. However, the diameters of the introduction port 56 need only be larger than the diameter of the exhaust port 57. Further, below the leukocyte removal filter 51, a discharge port 54 for discharging the concentrated platelet plasma after the leukocyte removal is provided.

  In the leukocyte removal filter unit 50, the bag 52 and the leukocyte removal filter 51 communicate with each other through the introduction port 56 and the exhaust port 57, so that the concentrated platelet plasma stored in the bag 52 is reliably transferred to the leukocyte every cycle. It can introduce into the removal filter 51 and can filter. Since the bag 52 as a reservoir is a variable container, the air in the blood component collection circuit 1 can also be processed.

  Moreover, the inflow port 65 can also be provided above the bag 52a like the leukocyte removal filter unit 50a shown in FIG. Also in this case, the opening (inlet) 65a of the inflow port 65 is installed above the liquid level of the concentrated platelet plasma stored in the bag 52a. By providing the inflow port 55a above the bag 52a in this way, it is not necessary to provide the partition wall 59.

Here, as a constituent material of each tube 25a, 25b, 26a, 26b, 26c connected to each tube used for formation of the 1st-3rd lines 21-23 mentioned above and each bag 25, 26, it is. Polyvinyl chloride is preferred. If these tubes are made of polyvinyl chloride, sufficient flexibility and softness can be obtained, so that they are easy to handle and are suitable for clogging with a clamp or the like.
Moreover, the same constituent material as each tube can be used also about the constituent material of each branch connector 21c, 21f, 22a mentioned above, respectively.

  As the plasma collection bag 25 and the platelet collection bag 26, resin-made flexible sheet materials are stacked, and the peripheral portions thereof are fused (thermal fusion, high frequency fusion, ultrasonic fusion, etc.) or bonded. What is used as a bag by bonding with an agent is used. As a material used for each bag 25, 26, for example, soft polyvinyl chloride is preferably used.

  In addition, as a sheet material used for the platelet collection bag 26, it is more preferable to use a material excellent in gas permeability in order to improve platelet storage stability. As such a sheet material, for example, polyolefin, DnDP plasticized polyvinyl chloride, or the like is used, and a sheet material of the above-described material is used without using such a material. What was thin (for example, about 0.1-0.5 mm, especially about 0.1-0.3 mm) is suitable.

  Next, the operation when collecting platelets using the blood component collection circuit 1 described above will be described. First, the blood component collection circuit 1 is attached to the blood component collection device, and the tube near the base of the blood collection needle 30 is clamped. Next, the anticoagulant container connecting needle 23d is connected to the anticoagulant container, the priming operation of the blood component collecting device is performed, and the anticoagulant liquid is filled to the lower part of the bubble removing chamber 23c. Thereafter, the clamp performed near the blood collection needle 30 is opened, and the needle tip of the blood collection needle 30 is filled with an anticoagulant.

  Then, the blood collection needle 30 is punctured into the blood vessel (vein) of the blood donor and blood collection is started. Simultaneously with the blood collection, an anticoagulant such as an ACD-A solution is supplied through the third line 23 by means of a pump or the like, and this anticoagulant is mixed into the collected blood. At this time, the anticoagulant is mixed with the collected blood while maintaining the mixing ratio with the blood at a constant value.

  In this way, the collected blood (anticoagulated whole blood) is transferred via the first line 21 and introduced into the centrifuge 20 from the inlet 143. At this time, since the centrifugal separator 20 is rotating, the components in the blood are separated into plasma, buffy coat containing platelets and white blood cells, and red blood cells from the direction near the center of the rotation axis by centrifugal force. When the volume of blood introduced into the centrifuge 20 exceeds the capacity of the centrifuge 20, first, plasma is discharged from the discharge port 144 of the centrifuge 20, and the second line 22 and the second tube 25b. Is stored in the plasma collection bag 25.

  When blood is further introduced into the centrifuge 20, the interface of plasma, buffy coat, and red blood cells formed in the centrifuge 20 approaches the center of the centrifuge 20. Detected by a sensor (not shown) provided in the tube, when the interface comes to an appropriate position, the clamp provided in the blood component collection circuit 1 is switched, and the plasma previously stored in the plasma collection bag 25 is centrifuged. While being introduced from the inlet 143 of the separator, the flow rate is increased to an appropriate flow rate, and the platelets of the buffy coat layer are discharged from the outlet 144 of the centrifuge 20.

  The exhausted concentrated platelet plasma is guided to the reservoir 42 via the second line 22 and the third tube 26a. When the concentrated platelet plasma stored in the reservoir 42 reaches a desired volume per cycle (for example, about 30 to 50 mL), the rotation of the centrifuge 20 is stopped and red blood cells are returned to the blood donor. . Thereafter, blood collection is started again, and the above-described steps are repeated until a desired total amount (for example, about 100 to 250 mL) of platelets is collected.

Here, since the cross-sectional area (opening area) of the communication path 46 for communicating the reservoir 42 and the leukocyte removal filter 41 is 0.7 cm ² or more, the inlet side of the leukocyte removal filter 41 is replaced with air every cycle. Even so, the concentrated platelet plasma stored in the reservoir 42 can be reliably introduced into the leukocyte removal filter 41 and filtered. Therefore, the concentrated platelet plasma introduced into the reservoir 42 for each cycle is sequentially introduced into the leukocyte removal filter 41 by a drop, and the leukocytes are removed by the leukocyte removal filter 41. The platelets from which the white blood cells have been removed are stored in the platelet collection bag 26.

  Thus, in the blood component collection circuit 1 according to the present embodiment, the concentrated platelet plasma collected in each cycle is stored in the reservoir 42 and filtered by the leukocyte removal filter 41 each time. For this reason, the filtration of concentrated platelet plasma for one cycle may be completed between the return of blood in the cycle and the collection of platelets in the next cycle. Therefore, even if it is the same work time as before, the flow rate at the time of passing the leukocyte removal filter 41 can be set low. As a result, leukocytes can be efficiently removed, so that high-quality platelets can be collected. Moreover, when the removal performance equivalent to the conventional one is sufficient, the leukocyte removal filter 41 can be reduced in size.

  As described above in detail, the blood component collection circuit 1 according to the present embodiment includes the reservoir 42 capable of storing the concentrated platelet plasma centrifuged by the centrifuge 20 for one collection cycle, and the white blood cells. A leukocyte removal filter unit 40 integrated with the leukocyte removal filter 41 to be removed is provided. Thus, for each collection cycle, the concentrated platelet plasma separated by the centrifuge 20 is temporarily stored in the reservoir 42, and the concentrated platelet plasma is introduced into the leukocyte removal filter 41 to remove leukocytes. be able to. The filtration of the concentrated platelet plasma by the leukocyte removal filter 41 may be performed between the return of the current cycle and the collection of the concentrated platelet plasma in the next cycle. Therefore, the concentrated platelet plasma when passing through the leukocyte removal filter 41 is used. The leukocyte removal performance of the leukocyte removal filter 41 can be improved. Moreover, the work time does not increase. Therefore, according to the blood component collection circuit 1 according to the present embodiment, it is possible to remove the white blood cells in the concentrated platelet plasma with high accuracy without extending the working time.

Further, the reservoir 42 is provided with an air vent filter 43 that is a breathable and bacteria-impermeable filter, and the air in the blood component collection circuit 1 can be taken in and out, so that no pressure fluctuation occurs in the circuit 1. .
Further, since the opening area of the communication path 46 between the reservoir 42 and the leukocyte removal filter 41 is 0.7 cm ² or more, even if the inlet side of the leukocyte removal filter 41 is replaced with air every cycle, the reservoir 42 The stored concentrated platelet plasma can be introduced into the leukocyte removal filter 41 and filtered.
Furthermore, since the reservoir 42 and the leukocyte removal filter 41 are unitized, the operation of attaching or removing the blood component collection circuit does not become complicated.

  It should be noted that the above-described embodiment is merely an example and does not limit the present invention in any way, and various improvements and modifications can be made without departing from the scope of the invention. In the above-described embodiment, the circuit for collecting the platelet preparation is exemplified, but the present invention can be applied to a circuit for collecting other blood components.

DESCRIPTION OF SYMBOLS 1 Blood component collection circuit 20 Centrifuge 21 1st line 22 2nd line 25 Plasma collection bag 26 Platelet collection bag 40 Leukocyte removal filter unit 41 Leukocyte removal filter 42 Reservoir

Claims (8)

A blood component collection circuit that separates blood into a plurality of blood components and collects the separated blood components,
A centrifuge for separating blood into a plurality of blood components;
A first line for flowing blood into the inlet of the centrifuge;
A second line connected to the outlet of the centrifuge;
A blood component reservoir that is connected to the second line and temporarily stores concentrated platelet plasma among the blood components separated by the centrifuge;
A cell separation filter connected to the blood component reservoir and separating and removing white blood cells from the concentrated platelet plasma;
A blood component collection bag connected to the cell separation filter and storing the concentrated platelet plasma after passing through the cell separation filter;
As a connection passage between the blood component reservoir and the cell separation filter, an inflow passage through which the concentrated platelet plasma flows into the cell separation filter and a discharge passage through which air from the cell separation filter is discharged are provided independently. A blood component collection circuit characterized by comprising: In the blood component collection circuit according to claim 1,
The blood component collection circuit according to claim 2, wherein the inlet of the blood component reservoir in the second line is provided above the liquid level of the concentrated platelet plasma stored in the blood component reservoir. In the blood component collecting circuit according to claim 1 or 2,
A blood component collecting circuit, wherein the volume of the blood component reservoir is variable. The blood component collection circuit according to any one of claims 1 to 3,
A blood component collection circuit, wherein a discharge port in the blood component storage part of the discharge path is provided above a liquid level of the concentrated platelet plasma stored in the blood component storage part. Any one blood component collection circuit according to any one of claims 1 to 4;
A clamp provided in the blood component collection circuit,
A blood component collection apparatus that separates blood into a plurality of blood components by the centrifuge and collects concentrated platelet plasma out of the separated blood components and performs a plurality of cycles of returning red blood cells,
By switching the clamp, the concentrated platelet plasma separated by the centrifuge every cycle is stored in the blood component reservoir, and the stored concentrated platelet plasma passes through the cell separation filter every cycle. And collecting the blood component in the blood component collection bag. A blood reservoir for temporarily storing blood;
A cell removal filter unit connected to the blood reservoir and having a cell separation filter for separating and removing predetermined cells from the blood,
An inflow port for allowing the blood to flow into the blood reservoir,
An inflow path through which the blood flows from the blood reservoir to the cell separation filter;
An outflow port for outflowing the blood that has passed through the cell separation filter;
A cell removal filter unit comprising: a discharge path for discharging air from the cell separation filter to the blood reservoir. The cell removal filter unit according to claim 6,
The cell removal filter unit, wherein a discharge port in the blood storage part of the discharge path is provided above a liquid level of the blood stored in the blood storage part. A cell removal filter unit according to any one of claims 6 or 7,
The cell removal filter unit, wherein an inlet of the blood storing part of the inflow port is provided above a liquid level of the blood stored in the blood storing part.

Images