RU2268055C1 - Method for obtaining disinfecting powder of fibrin - Google Patents

Abstract

FIELD: medicine, hematology.

SUBSTANCE: the present innovation deals with developing new ways for obtaining fibrin preparations. Method for obtaining disinfecting powder of fibrin out of human and animal blood clots deals with drying and mechanical reducing, moreover, raw mass of fibrin should be precept for 3-5 d in 0.05%-chlorohexidine bigluconate solution at 20-25°C. The innovation enables to obtain sterile powder of fibrin protected against the action of microorganisms, out of blood clots.

EFFECT: higher efficiency.

Description

The invention relates to medicine, in particular to hematology, and for the development of new methods for producing therapeutic preparations from human and animal blood and improving the quality of the proposed preparations.

A developed and proposed method for producing fibrin powder is its isolation from a blood clot, followed by drying, mechanical grinding and sterilization [1]. However, the proposed method has disadvantages due to the fact that the fibrin powder obtained by this method is a protein that is not protected from the action of microorganisms. Therefore, after applying it to the wound surface, it can be destroyed under the action of the microflora of the wound, and a protective fibrin crust, under which epithelization occurs, is not formed. In addition, this method includes a sterilization step.

The purpose of the invention is to obtain fibrin powder with the exception of the sterilization step, which includes disinfectants that do not interfere with the polymerization of fibrin into a fibrin crust, but which prevent the development of microflora.

The goal is achieved as follows. After examining donors for the carriage of HIV and hepatitis B and C viruses, blood is collected in sterile bottles, and animals are collected in clean dishes during slaughter. After blood coagulation, fibrin is isolated from blood clots. The obtained white fibrin flakes are washed with distilled water, filtered, squeezed from the remaining water and dried with filter paper. After that, the resulting mass of fibrin is placed in a vessel and a 0.05% chlorhexidine bigluconate solution is added based on 1 g of fibrin, 3.0-4.0 ml of solution. The resulting mass is kept for 3-5 days at a temperature of 20-25 ° C, filtered, and then the fibrin is dried at a temperature of 35-40 ° C until the liquid is completely removed. After drying, the fibrin is mechanically converted to powder.

The characteristics of the final product. The obtained fibrin powder has a grayish color, odorless, does not dissolve in water, but when mixed with water with subsequent drying it turns into a dense crust. To test its disinfectant properties, sterility was inoculated from the powder. 5-fold inoculation on the microflora gave a negative result.

Using the present invention allows to obtain a sterile powder of fibrin, protected from the action of microflora with a disinfectant, and to exclude the stage of sterilization.

Literature

1. A method of producing fibrin powder. Patent No. 1811845. Authors: V.A.Figurnov, E.V.Figurnova.

Claims (1)

A method of obtaining a disinfectant fibrin powder from blood clots of humans and animals, followed by drying and mechanical grinding, characterized in that the wet mass of fibrin is pre-incubated for 3-5 days in a 0.05% solution of chlorhexidine bigluconate at a temperature of 20-25 ° C.