RU2243246C2 - Method of preparing basic dye for morphologic applications - Google Patents

Abstract

FIELD: biochemical methods.

SUBSTANCE: basic dye for histological application is prepared from vegetable raw material, in particular onion peel, which is preliminarily disintegrated and then extracted with boiling water. Extract solution is filtered and evaporated. 4-5 g of dry extract is dissolved in 100 ml of 70% ethanol, after which 12 g of potassium alum and 10 ml of ice acetic acid are added to give desired dye.

EFFECT: simplified preparation procedure.

1 dwg

Description

The invention relates to the biological industry, and in particular to a method for producing a basic dye for morphological purposes, which can be used for staining histological preparations.

A known method of producing a natural dye from onion husks, involving extraction with water and filtering the extract (RU 2021310, C1, 1994), as well as a dye based on onion husks with crushed bark of coniferous trees (RU 2041899, C1, 1995).

However, these dyes are intended for the food and textile industries.

Closer in technical essence is a method for producing a histological dye disclosed in RU 2061005, C1, 1996.

The known method involves obtaining a dye for histological purposes from plant material - the fruit of the raven eye, which includes grinding plant material, extraction of 10-12 vol. solvent - 70% ethyl alcohol with stirring, filtering the obtained extract, acidifying the obtained extract with glacial acetic acid and adding to it a 0.05% solution of hyaluronic acid.

However, in a known manner, a histological dye is obtained in a solution that has a limited shelf life, after which the coloring property of the dye deteriorates.

These disadvantages are eliminated by the invention.

The aim of the invention is to increase the coloring properties of the main dye, on the basis of integumentary scales of onion bulbs, suitable for morphological purposes.

According to the invention, a method for producing a basic dye from plant materials for histological purposes is provided, including grinding plant materials, extraction and filtration, moreover, husk coverslips of onion bulbs are used as plant materials, extraction is carried out with water while boiling, and after filtration, the resulting extract is subjected to dehydration by evaporation with the subsequent preparation of the target dye by dissolving 4-5 g of dry extract in 100 ml of 70% ethanol and adding 12 g of feces ynyh alum and 10 ml of glacial acetic acid. After chopping, boiling, setting and filtering, the onion bulb coverslips are dehydrated by evaporating the extract in an oven to obtain a dry, powdery dye.

The main dye is prepared from the obtained dry powder on the basis of covershells of onion bulbs. To 100 ml of 70% ethanol add 4-5 g of dry dye, as well as 10 ml of glacial acetic acid and 12 g of potassium alum.

Example. Peeled and crushed integumentary scales of onion bulbs are poured with water, boiled for 4 hours, infused for a day. The resulting solution is filtered, dehydrated in an oven in a spacious container until completely dehydrated. The resulting dark brown powder is suitable for morphological studies, while 4-5 g of the obtained dry dye are dissolved in 100 ml of 70% ethanol, followed by the addition of 10 ml of glacial acetic acid and 12 g of potassium alum.

Claims (1)

A method of obtaining the main dye from plant material for histological purposes, including grinding plant material, extraction and filtration, characterized in that the husk of onion onion flakes is used as plant material, extraction is carried out with water during boiling, and after filtration, the resulting extract is subjected to dehydration by evaporation with the subsequent preparation of the target dye by dissolving 4-5 g of dry extract in 100 ml of 70% ethanol and adding 12 g of potassium alum and 10 m glacial acetic acid.