CN114617193A - Preparation method of eupatorium adenophorum feed - Google Patents

Preparation method of eupatorium adenophorum feed Download PDF

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Publication number
CN114617193A
CN114617193A CN202210104147.XA CN202210104147A CN114617193A CN 114617193 A CN114617193 A CN 114617193A CN 202210104147 A CN202210104147 A CN 202210104147A CN 114617193 A CN114617193 A CN 114617193A
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percent
eupatorium adenophorum
small molecule
feed
molecule fracture
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皋玉功
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Eupatorium Adenophorum Traditional Chinese Medicine Agricultural Technology Yunnan Co ltd
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Eupatorium Adenophorum Traditional Chinese Medicine Agricultural Technology Yunnan Co ltd
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Priority to CN202210104147.XA priority Critical patent/CN114617193A/en
Publication of CN114617193A publication Critical patent/CN114617193A/en
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/10Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • A23K10/18Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K40/00Shaping or working-up of animal feeding-stuffs
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/70Feeding-stuffs specially adapted for particular animals for birds
    • A23K50/75Feeding-stuffs specially adapted for particular animals for birds for poultry
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L5/00Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
    • A23L5/20Removal of unwanted matter, e.g. deodorisation or detoxification
    • A23L5/28Removal of unwanted matter, e.g. deodorisation or detoxification using microorganisms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/11Lactobacillus
    • A23V2400/113Acidophilus
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/51Bifidobacterium

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Animal Husbandry (AREA)
  • Physiology (AREA)
  • Biotechnology (AREA)
  • Birds (AREA)
  • Molecular Biology (AREA)
  • Nutrition Science (AREA)
  • Mycology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Botany (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

The invention discloses a preparation method of a eupatorium adenophorum feed, which comprises the following steps; (1) roughly pulverizing picked fresh Eupatorium adenophorum, detoxifying with microorganism, and mixing with 0.5% Glycyrrhrizae radix powder and 0.5% semen Phaseoli Radiati powder; (2) and (2) feeding the uniformly mixed material obtained in the step (1) into steam explosion equipment, pressurizing and heating for 5 minutes under 20 standard atmospheric pressures to explode the material into small molecule fracture shapes at one time, and then outputting the small molecule fracture shapes to a drying system to dry the small molecule fracture shapes until the water content is below 13%. Compared with the prior art, the invention has shorter detoxification treatment time and saves space conditions, thereby being beneficial to industrial large-scale production and use. By combining the current situation that the eupatorium adenophorum is widely distributed in southwest and southeast Asia areas of China, the plant is decomposed into the feed which can be used for animals in a mass production mode, and the method is a big strategy for solving the problem of killing the first plant in the world.

Description

Preparation method of eupatorium adenophorum feed
Technical Field
The invention belongs to the technical field of feeds, and particularly relates to a preparation method of a eupatorium adenophorum feed.
Background
Eupatorium adenophorum Spreng is a Eupatorium adenophorum plant of Compositae, commonly called as aircraft grass, is used as a killer in plant kingdom and also belongs to an invasive plant, is deeply harmed by agricultural land and forest land, is difficult to grow vegetation, is diseased with livestock, causes the reduction of yield of grains, and is widely threatened by ecology.
If the abused eupatorium adenophorum is completely eradicated manually and the pasture is replaced by the cultivated pasture, the investment and the cost of capital are huge, and the return is too low or too slow. There is a need for a way to eliminate Eupatorium adenophorum and then have the potential to produce higher economic value. The authorization notice number is CN1182790C, and the subject names are: the Chinese patent of the preparation method of the eupatorium adenophorum feed discloses a method for detoxifying the eupatorium adenophorum by using a detoxifying agent and then preparing the feed. The method has certain advancement, but also has the problems that the stacking fermentation is needed for 1 to 3 days, and the time and the space are wasted and the fund is wasted for the large-scale production for a longer time when the temperature is lower.
Disclosure of Invention
Aiming at the situation, the invention provides the preparation method of the eupatorium adenophorum feed, which solves the problem that the prior art needs long-time fermentation, and has the effects of good detoxification effect, thorough material cracking and contribution to absorption by poultry.
In order to achieve the purpose, the invention provides the following technical scheme: a preparation method of Eupatorium adenophorum feed comprises the following steps;
(1) coarsely crushing picked fresh eupatorium adenophorum;
(2) the solid microorganism detoxication agent consists of 40 percent of Bacillus licheniformis (Bacillus licheniformis),20 percent of Bacillus cereus, 20 percent of Lactobacillus acidophilus (Lactobacillus acidophilus) and 20 percent of Bifidobacterium (Bifidobacterium), and is soaked in warm water at the temperature of 25-30 ℃ for 1 hour, and then 0.5 percent of the solid microorganism detoxication agent, 0.5 percent of licorice powder and 0.5 percent of mung bean powder are added according to the weight of coarse powder wet material and are uniformly mixed with the wet material;
(3) and (3) feeding the uniformly mixed material obtained in the step (2) into steam explosion equipment, pressurizing and heating for 5 minutes under 20 standard atmospheric pressures to explode the material into small molecule fracture shapes at one time, and then outputting the small molecule fracture shapes to a drying system to dry the small molecule fracture shapes until the water content is below 13%.
Compared with the prior art, the invention has the beneficial effects that:
firstly, the eupatorium adenophorum spreng and the detoxication agent are mixed without stacking and fermenting, and are directly put into steam explosion equipment to be subjected to steam explosion for five minutes under 20 standard atmospheric pressures, compared with the prior art, the detoxication treatment time is shorter, and the industrial large-scale production and use are facilitated; meanwhile, the space and the labor capital cost required by stacking fermentation are saved.
And secondly, compared with the prior art, the content of the nutrient components of the eupatorium adenophorum material after steam explosion is obviously improved, the content of total flavone, total phenol and total sugar is obviously improved, the application of the flavonoid compound can obviously improve the production performance of animals, the disease resistance of the animal body and the immune function of the animal body, the total phenol has an anti-oxidation effect and can play a certain role in preventing diseases of poultry, and the improvement of the total sugar can provide more energy for the poultry.
Detailed Description
The technical solutions in the embodiments of the present invention are clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments; all other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In one embodiment, a method for preparing a eupatorium adenophorum feed comprises the following steps;
(1) coarsely crushing picked fresh eupatorium adenophorum;
(2) the solid microorganism detoxication agent consists of 40 percent of Bacillus licheniformis (Bacillus licheniformis),20 percent of Bacillus cereus, 20 percent of Lactobacillus acidophilus (Lactobacillus acidophilus) and 20 percent of Bifidobacterium (Bifidobacterium), and is soaked in warm water at the temperature of 25-30 ℃ for 1 hour, and then 0.5 percent of the solid microorganism detoxication agent, 0.5 percent of licorice powder and 0.5 percent of mung bean powder are added according to the weight of coarse powder wet material and are uniformly mixed with the wet material;
(3) and (3) feeding the uniformly mixed material obtained in the step (2) into steam explosion equipment, pressurizing and heating for 5 minutes under 20 standard atmospheric pressures to explode the material to break the material into small molecules in a breaking state at one time, and then outputting the small molecules to a drying system to be dried until the water content is below 13%.
Example two, determination of total flavonoids, total phenols and total sugars:
determination of Total Flavonoids concentration
Weighing 100g of powder, adding 1500ml of 60% ethanol solution according to the material-liquid ratio of 1:15(m/v), carrying out ultrasonic treatment in a water bath at 80 ℃ for 2h, filtering, extracting with petroleum ether to remove ester substances, taking 1ml of lower-layer liquid, putting into a volumetric flask, adding 3ml of 60% ethanol, adding 1ml of 5% sodium nitrite solution, shaking up, standing for 6min, adding 1ml of 10% aluminum nitrate solution, shaking up, standing for 6min, adding 10ml of 4% sodium hydroxide, fixing the volume with 60% ethanol solution to 25ml of volumetric flask, standing for 15min, carrying out centrifugal filtration, taking 60% ethanol solution as a blank, and measuring the absorbance at 510 nm.
Standard curve a ═ 0.002C-0.0003(ug/ml) R2 ═ 0.9995
Figure RE-GDA0003643385410000031
Determination of the Total phenol concentration
The sample solution is extracted as flavone, 1ml of extracting solution is added with 5ml of water, 1ml of FC color developing agent and 3ml of 7.5% sodium carbonate, 6ml of water, 1ml of FC color developing agent and 3ml of 7.5% sodium carbonate are added into blank control, the mixture is mixed and kept stand for 2h after being sequentially added into the solution, and the absorbance is measured at 765 nm.
Standard mark A ═ 0.0011C-0.0054(ug/ml) R2 ═ 0.9994
Figure RE-GDA0003643385410000032
Figure RE-GDA0003643385410000041
Determination of the Total sugar concentration
Weighing 0.2g sample, grinding into homogenate, pouring into a triangular flask, washing the bowl with 10ml 80% ethanol solution, collecting the solution in the triangular flask, and water-bathing at 70-80 deg.C for 40min while stirring. Centrifuging, collecting supernatant, stirring the residue with about 10ml 80% ethanol solution, water bathing at 70-80 deg.C for 40min, and centrifuging to obtain supernatant. Mixing the two supernatants, diluting with 80% ethanol solution to 50ml, adding 10ml activated carbon, decolorizing in 70-80 deg.C water bath for 30min, filtering, and collecting filtrate for determination. Sucking 1ml of sugar sample extract, adding anthrone reagent, mixing, immediately placing in boiling water bath for 10min, taking out, cooling, and measuring absorbance value with spectrophotometer at wavelength of 625 nm.
Bistringa 0.0088C-0.0126R2 0.9993
Figure RE-GDA0003643385410000042
Figure RE-GDA0003643385410000051
In summary, the following steps: the contents of total flavone, total phenol and total sugar in the eupatorium adenophorum material after steam explosion are obviously improved compared with the contents of the product after the stacking fermentation treatment in the prior art without fermentation treatment.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims (1)

1. A preparation method of Eupatorium adenophorum feed comprises the following steps;
(1) coarsely crushing picked fresh eupatorium adenophorum;
(2) the solid microorganism detoxication agent consists of 40 percent of Bacillus licheniformis (Bacillus licheniformis),20 percent of Bacillus cereus, 20 percent of Lactobacillus acidophilus (Lactobacillus acidophilus) and 20 percent of Bifidobacterium (Bifidobacterium), and is soaked in warm water at the temperature of 25-30 ℃ for 1 hour, and then 0.5 percent of the solid microorganism detoxication agent, 0.5 percent of licorice powder and 0.5 percent of mung bean powder are added according to the weight of coarse powder wet material and are uniformly mixed with the wet material;
the method is characterized in that: and also comprises
(3) And (3) feeding the uniformly mixed material obtained in the step (2) into steam explosion equipment, pressurizing and heating for 5 minutes under 20 standard atmospheric pressures to explode the material into small molecule fracture shapes at one time, and then outputting the small molecule fracture shapes to a drying system to dry the small molecule fracture shapes until the water content is below 13%.
CN202210104147.XA 2022-01-28 2022-01-28 Preparation method of eupatorium adenophorum feed Pending CN114617193A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2023151294A1 (en) * 2022-02-09 2023-08-17 紫茎泽兰中医农业科技(云南)有限公司 Non-grain type feed for monogastric animals and preparation method therefor

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1383731A (en) * 2002-05-18 2002-12-11 欧阳华 Production process of purple eupatoria feed
CN205455956U (en) * 2016-01-19 2016-08-17 方珣 Device that explodes production plant fodder is spouted in pressurization of cooking in succession

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1383731A (en) * 2002-05-18 2002-12-11 欧阳华 Production process of purple eupatoria feed
CN205455956U (en) * 2016-01-19 2016-08-17 方珣 Device that explodes production plant fodder is spouted in pressurization of cooking in succession

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
方芳: "蒸汽爆破预处理对籽粒苋籽实抗氧化能力的影响", 食品工业科技, vol. 39, no. 15, pages 122 - 123 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2023151294A1 (en) * 2022-02-09 2023-08-17 紫茎泽兰中医农业科技(云南)有限公司 Non-grain type feed for monogastric animals and preparation method therefor

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