RU2232501C2 - Method for increasing natural resistance in animals - Google Patents

Abstract

FIELD: animal science.

SUBSTANCE: for the purpose to solve the problem of prophylaxis and complex therapy of different animal disease, such as bronchopneumonia, intestinal infections, hepatitis, wounds and operational sutures complicated with purulent infection and others it is suggested to inject immunostimulating preparation consisting of ACD-f2, ascorbic acid, cyancobalamin, sodium chloride, potassium chloride, sodium hydrocarbonate and distilled water. His preparation should be injected intramuscularly - at the age of 1 mo and 5 mo. The method enables to increase bactericidal and lysozymic activity of blood serum in animals. It, also, stimulates their growth and development.

EFFECT: higher efficiency.

2 ex, 3 tbl

Description

The invention relates to veterinary medicine, namely to increasing the general non-specific resistance in animals, and can be used for the prevention of various diseases of infectious and non-infectious etiology, in the treatment of the same diseases, as well as to increase the weight gain of animals that are fed.

Known use of the drug “mastim” (Veterinary drugs in Russia: a Handbook, 2000), consisting of biogenic stimulants of tissue origin and biologically active substances (ASD f-2, ascorbic acid, sodium chloride). However, “mastim” is used to stimulate immunological reactivity and regenerative processes that occur in the animal’s body during the period of illness with diseases of infectious and non-infectious etiology, in addition, “mastim” showed low stimulating activity, as well as the need for large doses of administration.

The authors were faced with the task of prevention and complex therapy of various diseases, such as bronchopneumonia, intestinal infections, hepatitis, complicated by purulent infection of the wound, surgical sutures, etc., as well as cost reduction due to the shorter period of the disease of animals and the use of small doses of the drug.

This problem was solved by administering to animals an immunostimulating drug consisting of f-2 ASD - 10000 ± 100 mg / 100 ml, ascorbic acid - 3000 ± 50 mg / 100 ml, cyancobalamin - 15 ± 1 mg / 100 ml, sodium chloride - 500 ± 25 mg / 100 ml, potassium chloride - 100 ± 5 mg / 100 ml, sodium bicarbonate - 400 ± 20 mg / 100 ml and distilled water - the rest (up to 100 ml). We offer to call our drug “Vitstim”.

At the same time, for the prevention of calf diseases and for obtaining additional gains, the introduction is carried out in two stages. The first - at the age of one month - five injections in three days at 2.5 ml per head, the second - at the age of 5 months - five injections in three days at 5.5 ml per head.

The method of use and preparation of the drug is presented by examples 1,2 and tables 1,2.

Example 1. To prepare 100 ml of the drug, 10 ml of f-2 ASD, 3 g of ascorbic acid, 15 mg of cyancobalamin, 500 mg of sodium chloride, 100 mg of potassium chloride, 400 mg of sodium bicarbonate are necessary and the solution volume is brought to 100 ml with distilled water. The ingredients of the preparation were mixed in the following sequence: first, distilled water was introduced into a sterile flask, in which sodium chloride, potassium chloride, sodium bicarbonate, ascorbic acid and cyancobalamin were successively dissolved. Then, after thorough dissolution of the above components, ADS f-2 was added.

To prevent diseases, increase immunity, this drug is administered intramuscularly at a dose of 0.02-0.1 ml per kg of animal weight with an interval of 1-5 days, 5-6 injections in a row.

Example 2. To compare the effectiveness of the proposed drug used a similar drug mastim, consisting of ASD f-2, ascorbic acid and physiological saline.

For this purpose, three groups of calves were formed at the age of one month, 7 animals each. The first group was the control. The second group was injected intramuscularly with mastim at a dose of 3.5 ml per head every three days five injections. And the third is a test drug of 2.5 ml per head every three days with five injections.

At the age of five months, repeated injections of the above drugs were carried out at the same interval, but only the second group was injected with 7.0 ml per head, and the third group 5.5 ml per head. The effectiveness of the drug was judged by hematological parameters, bactericidal and lysozyme activity of blood serum, as well as weighed and determined average daily gains. The bactericidal activity of blood serum was determined according to the method developed by O.V.Smirnova and T.A. Kuzmina (1966), lysozyme activity by the method proposed by Yu.M. Markov et al. (1974). During the development of calves, they revealed different dynamics of hematological parameters and growth energy (Table 1-2).

The number of red blood cells in calves of the third group is greater than that of peers of the first and second groups at 1.5-17.5%. According to hemoglobin, the difference in favor of animals of the third group was 2.7-17.5%. The bactericidal and lysozyme activity of blood serum in all groups increases evenly, but a more intensive growth of these indicators is in the third group.

In addition, bulls of the third group in all periods of the study exceeded peers of other groups in growth energy by 7-25%. They daily increased live weight by 60-170 g more than analogues of other groups. Therefore, at 215 days, their live weight averaged 210 kg, or 11-20 kg more than the calves of other groups.

As follows from the data presented in the tables, as a result of exposure to the test drug in the body of the calves, changes occur, indicating an increase in natural resistance, which subsequently leads to a more rapid increase in body weight,

Due to the fact that in the third group, higher gains were obtained and less test drug was spent, this led to a reduction in unit costs.

This drug has also been tested on dogs, cats, pigs and horses. In all animal species, the bactericidal and lysozyme activity of blood serum significantly increased, and piglets gained weight faster than their peers. Summarizing the data obtained on the above animals, the average doses of the drug were derived depending on the weight (Table 3).

Based on the foregoing and based on the results of the patent information search, we believe that our proposed “Method for increasing the natural resistance of animals” meets the criteria of “novelty”, “inventive step”, “applicability” and can be protected by a patent of the Russian Federation.

Claims (1)

A method of increasing the natural resistance of animals, which consists in the administration of an immunostimulating drug containing ASD f-2, ascorbic acid and sodium chloride, characterized in that the preparation additionally contains cyancobalamin, potassium chloride, sodium bicarbonate, distilled water and components are taken in the following ratio, mg / 100 ml: ASD f-2 10000 ± 100 Ascorbic acid 3000 ± 50 Cyanocobalamin 15 ± 1 Sodium Chloride 500 ± 25 Potassium chloride 100 ± 5 Sodium bicarbonate 400 ± 20 Distilled Water Else moreover, the drug is administered twice - for the first time at the age of five months, five injections in three days at 2.5 ml per head, and again at five months of age five injections in three days at 5.5 ml per head.