RU2224508C2 - Preparation for treating purulent-necrotic lesions of limbs in cattle and method for its application - Google Patents

Abstract

FIELD: veterinary science. SUBSTANCE: the present innovation deals with antimicrobial preparations applied for treating purulent-necrotic lesions of limbs in cattle and methods for their applications. The suggested preparation includes antimicrobial substance of tetracyclines' group represented by foundation's oxyteteracycline salt. Moreover, additionally, the preparation contains Novocain, sodium sulfite (or sodium formaldehyde sulfoxylate), magnesium chloride, polyvinyl alcohol, propylene glycol and distilled water at the following ratio of components, g/l solution: oxytetracycline hydrochloride 120-160, novocaine 1.5- 2.5, sodium sulfite or sodium formaldehyde sulfoxylate 4.0-6.0, magnesium chloride 12-16, polyvinyl alcohol 30-40, propylene glycol 80-120, distilled water the rest. Method for treating purulent-necrotic lesions of limbs in cattle deals with introducing medicinal preparation at the dosage of 1 ml/7 kg body weight twice at 7-d-long interval into affected limb, moreover, injection should be performed into soft tissues of affected limb being above lesion site into several points. The present innovation enables to shorten terms of recovery by 1.5 times and increase efficiency of therapy by 116.4-18.1%. EFFECT: higher efficiency of therapy. 3 cl

Description

 The invention relates to the field of veterinary medicine, in particular to antimicrobial agents used for the treatment of purulent-necrotic lesions of the limbs in cattle, and methods for their use.

 Known drugs for the treatment of purulent-necrotic lesions of the extremities in cattle - antibiotics of the penicillin and tetracycline series and methods of use (Diagnosis, prevention and control measures for necrobacteriosis of cattle. - M .: MBA. 2000. - S. 10).

 The disadvantage of these drugs is that they are administered 2-4 times a day, which is quite difficult and time-consuming. Their treatment is long and not always effective.

 The closest solution adopted for the prototype is a well-known drug for the treatment of purulent-necrotic lesions of the extremities in cattle - levotetrasulfin and the method of its application (Rules for the prevention and elimination of animal necrobacteriosis. - M.: Ministry of Agriculture of the Russian Federation, 2001. - P. 5 ), which is used in a dose of 0.2-0.3 ml per 1 kg of body weight 2-3 times with an interval of 4-6 days.

 The disadvantages of this drug are its insufficiently high efficiency in purulent necrotic lesions of the limbs in cattle, a large administered dose and a long course of treatment.

 The basis of the invention is the task of creating such an antimicrobial drug for the treatment of purulent-necrotic lesions of the extremities in cattle and the method of its use with such active substances, solvents and an auxiliary component that, acting in combination, can have an effective antimicrobial therapeutic effect in the foci of purulent necrotic lesions.

The problem is solved in that the antimicrobial preparation for treating purulent-necrotic lesions of the limbs in cattle, containing the antimicrobial substance according to the invention, contains oxytetracycline hydrochloride as antimicrobial substances, novocaine, sodium sulfite (or sodium formaldehyde sulfoxylate) are used as other active substances , magnesium chloride, propylene glycol and distilled water were used as solvents, watering was used as auxiliary substances Nilova alcohol, with the following component ratio, g / l solution:
Oxytetracycline hydrochloride - 120-160
Novocaine - 1.5-2.5
Sodium sulfite or sodium formaldehyde sulfoxylate - 4.0-6.0
Magnesium Chloride - 12-16
Polyvinyl alcohol - 30-40
Propylene glycol - 80-120
Distilled Water - Else
The problem is also solved by the fact that in the known method of treating purulent-necrotic lesions of the extremities of cattle, which consists in introducing into the soft tissue of the affected limb an antimicrobial preparation, according to the invention, the antimicrobial preparation is administered in a dose of 1 ml per 7 kg of body weight twice with interval of 7 days.

 The problem is also solved by the fact that the introduction of the drug is carried out in the soft tissue of the affected limb above the lesion at several points.

 The proposed drug is easy to use and does not require large labor costs from veterinarians.

The inventive antimicrobial preparation is obtained and used as follows:
To obtain 1.0 l of the antimicrobial drug, we take 400 ml of distilled water and bring to a boil. In boiling water, add 80-120 ml of propylene glycol and a suspension of polyvinyl alcohol (30-40 g per 350 ml of cold distilled water).

The resulting mixture of components is heated to 75 ^o C and dissolve the active substances in it in the following quantities, g:
Oxytetracycline hydrochloride - 120-160
Novocaine - 1.5-2.5
Sodium sulfite or sodium formaldehyde sulfoxylate - 4.0-6.0
Magnesium Chloride - 12-16
Then add distilled water to a volume of 1 liter.

 The resulting preparation is a viscous opaque solution from dark yellow to light brown in color, the presence of a yellow finely divided precipitate is permissible.

 The antimicrobial effect of the claimed drug was studied in an in vitro experiment on cultures of St. aureus, St. albus, Str. fecalis, P. vulgaris, F. necroforum isolated from foci of purulent-necrotic lesions of the extremities in cattle. The bacteriostatic effect of the drug was determined by the method of serial dilutions in liquid nutrient media and was evaluated by the minimum microbial growth inhibiting concentration (MZK) of the whole drug and its active antimicrobial substance. Two serial dilutions in water were prepared from the preparation, which were introduced into a liquid nutrient medium with a specific microbial culture. It was found that at nominal values of antimicrobial substances, the growth of the test microbes is suppressed in MZK 16-32 μg / ml. This indicates a high bacteriostatic activity of the drug - its MPC is 100-150 times lower than a single antimicrobial substance.

 A test of the therapeutic effect of the known and claimed drug was carried out on cattle with purulent-necrotic lesions of the extremities of mild and moderate severity. St. was isolated from the lesion aureus, St. albus, Str. fecalis, P. vulgaris, F. necroforum.

 In the first experimental group there were 126 cows. The animals used the preparation according to example 1 intramuscularly at several points of the diseased limb above the lesion area at a dose of 1 ml per 7 kg of body weight twice with an interval of 7 days.

 In the control group (54 heads), animals were treated with levotetrasulfine according to the instructions at a dose of 0.3 ml / kg body weight three times after 5 days.

 When using the claimed drug, recovery was observed on days 7-9 in 75 animals (59.5%), on days 10-12 in 26 animals (20.6%), on days 13-15 in 13 animals (10.3%) . A total of 114 cows recovered (90.5%).

 When using the well-known (control) drug, recovery was noted on days 10-12 in 24 animals (44.4%), on days 13-15 in 12 animals (22.2%), and later in 4 animals (7.4%). A total of 40 cows recovered (74.1%).

 Thus, the claimed drug provided recovery of 90.5% of patients within 7-14 days.

 The control drug levotetrasulfine was effective in 74.1% of cases and only for 10-20 days.

 The use of the claimed drug and method for the treatment of purulent-necrotic lesions of the limbs in cattle can increase the cost-effectiveness of treatment by reducing the recovery time by 1.5 times and increasing the effectiveness of treatment by 16.4-18.1%.

Claims (3)

1. A drug for the treatment of purulent-necrotic lesions of the extremities in cattle, including an antimicrobial substance of the tetracycline group, characterized in that the antimicrobial substance includes a salt of oxytetracycline base and, in addition, contains novocaine, sodium sulfite (or sodium formaldehyde sulfoxylate), magnesium chloride, polyvinyl alcohol, propylene glycol and distilled water in the following ratio of components, g / l of solution: Oxytetracycline hydrochloride 120-160 Novocaine 1.5-2.5 Sodium sulfite or sodium formaldehyde sulfoxylate 4.0-6.0 Magnesium chloride 12-16 Polyvinyl alcohol 30-40 Propylene glycol 80-120 Distilled water 2. A method of treating purulent-necrotic lesions of limbs in cattle, comprising administering a drug to the affected limb, characterized in that the drug according to claim 1, which is administered at a dose of 1 ml per 7 kg of body weight twice with interval of 7 days. 3. The method according to claim 2, characterized in that the introduction of the drug is produced in the soft tissue of the affected limb above the lesion at several points.