RU2216346C1 - Preparation and method for biogenic body stimulation - Google Patents

Abstract

FIELD: medicine. SUBSTANCE: the present innovation deals with biomedicinal chemistry of natural proteins and elaboration of biogenic stimulant of new generation and, also, method for biogenic body stimulation for the purpose to correct its state. The suggested multi-purpose biogenic stimulant is a somatotropic hormone based upon natural, synthetic or recombinant preparation treated by incubation in darkness and under cold conditions for 3-6 h, and, also, method for biogenic body stimulation dealing with introduction of activated somatotropic hormone in combination with different biologically active substances, for example, vitamins or vitamin-like preparations. So, biogenic stimulant has been developed of broadened range of biologically active action, therapeutic and sanitation-cosmetic action to be used for obtaining different curative- sanitation effects. EFFECT: higher efficiency. 5 cl, 5 dwg, 36 ex

Description

 The invention relates to medicine, more specifically to the biomedical chemistry of natural proteins, and is associated with the development of a biogenic stimulator based on growth hormone, as well as the development of a method of biogenic stimulation of the body in need of correction of the condition.

 The name "biogenic stimulants" (BS) (translated from Greek as "bios" - life, "geneo" - I give birth) was proposed by academician V.P. Filatov (at the end of the 30s) for a group of substances formed under certain conditions in natural biosubstrates, for example, tissues of animal or plant origin, and which, when introduced into the body, have a stimulating effect and accelerate regeneration processes. The formation of these substances occurs mainly when tissues are placed in adverse conditions (cooling, exposure to various physical factors, etc.). The appearance of these substances is considered as a result of the adaptation of these biosubstrates.

 Biogenic stimulants used in modern medical practice include preparations from plants (aloe extract), animal tissues (placenta suspension), as well as from estuary mud (for example, peloidin, humisole) and peat (peat). It is believed that the formation of biologically active substances in estuary mud and peat is due to extinct microflora and microfauna. The mechanism of action of biogenic stimulants has so far been little studied [1].

 Currently BS is successfully used in medical practice. For example, plasmol for injection is obtained from human blood with the addition of 0.68% sodium chloride, it is a colorless or slightly opalescent transparent or slightly opalescent liquid with a specific odor, slightly salty taste, soluble in water; pH 6.0-7.0. Plazmol has a nonspecific desensitizing effect and anti-inflammatory effect. Plazmol is prescribed in the treatment of gastric ulcer and duodenal ulcer, asthma, radiculitis, neuralgia, neuritis occurring with pain, chronic inflammatory processes, arthritis and polyarthritis.

 Another well-known representative of the BS group is tintal. This drug is made on the basis of bioactivated aloe by a unique technique and is very effective in the following diseases: atherosclerosis, pneumonia, asthma, pulmonary tuberculosis, colds, bronchitis, tracheitis, gastric and duodenal ulcer, gastritis, enteritis, colitis, constipation hemorrhoids, heartburn. Cancer Prevention. Decreased visual acuity and eye diseases. Metabolic disorder, obesity. Skin diseases (psoriasis, eczema, furunculosis). The main action: increasing nonspecific resistance (natural defenses) of the body. Medical and preventive medicine shows particular interest in biogenic stimulants - substances that stimulate the protective and other adaptive properties of the body. The sources of such substances include tintal, prepared on the basis of aloe. Tintal has a wide spectrum of action, is devoid of allergenic properties and is safe to use. Using a unique method of bioactivation of aloe allows you to accumulate a high amount of BS in the tintal to sufficiently stimulate the body's defenses. Due to the ability to quickly repair damaged tissues, tintal can be used for a variety of inflammatory and non-inflammatory diseases. The use of tinctal significantly slows the development of atherosclerosis. In diseases of the bronchopulmonary system, the use of tinctal helps soften and quickly disappear cough, improve overall health, appetite and sleep, reduce weakness and shortness of breath. In case of diseases of the gastrointestinal tract shortly after the start of treatment, pain disappears, heartburn stops, healing of ulcers accelerates, digestion normalizes. Tintal is advisable to use in the complex treatment of patients with psoriasis, eczema and furunculosis. Installed. that bioactivated aloe is able to restore the integrity of the cells of the visual apparatus, so tintal is recommended for all eye diseases. Tintal has a calming effect on the nervous system, healing and rejuvenating effect on the skin.

 Another known BS is Suspensio Placentae pro injectionibus. A suspension of finely ground cold-preserved human placenta in an isotonic sodium chloride solution. Homogeneous (after shaking) a reddish-brown suspension with a characteristic odor; pH 5.8-6.9. It is used as a biogenic stimulant for various eye diseases (myopia, keratitis, clouding of the cornea, iritis, clouding of the vitreous body) and other indications for the use of biogenic stimulants [1].

 Our studies are not aimed at studying substances of tissue origin (animal or plant), but at studying a natural biosubstrate — substances of a protein structure (its structure is quite well known), whose properties are modified by additional factors. This allows you to use the new drug in many other directions to improve the condition of the body in which this substance has not been previously used. We are talking about growth hormone, a protein substance with a studied structure.

An object of the present invention is to provide a BS with an expanded spectrum of biologically active action, namely, possessing not only therapeutic, but also cosmetic and cosmetic effects, which can be used to obtain various therapeutic and therapeutic effects. These include:
Correction of the state of the body with gerontological problems
Fat loss
Correction under stress and exposure to short-wave frequencies (EHF)
Heart function improvement
Stimulation of the immune system
Improving physical performance and endurance
Kidney function improvement
Blood pressure normalization
An improvement in cholesterol with an increase in high-density lipid (HDL) and a decrease in low-density lipid (LDL)
Strengthening bones, accelerating bone healing in fractures
Accelerate Wound Healing
Rejuvenation, skin firming and thickening
Hair regrowth
Wrinkle reduction
Cellulite removal
Visual acuity
Mood boost
Improving memory, learning
Sleep normalization
Acceleration of regeneration of damaged brain tissue
The problem is solved by creating an activated drug based on protein - somatotropin, both natural and synthetic, or obtained by genetic engineering (recombinant).

 The initial drug is somatotropic hormone (SG) - a growth hormone secreted by the anterior pituitary gland, which is a polypeptide consisting of 191 amino acids. The main effect of SG is the stimulation of protein synthesis in the body, due to which its growth action is carried out. In contrast to sex hormones, SH enhances skeletal growth, but does not accelerate the rate of ossification of growth zones. Its introduction to animals is accompanied by increased growth, as a result of which large individuals appear. A person’s growth depends on the activity of his SG, which is determined by hereditary factors.

 SG reveals a wide range of biological effects in the body, which is manifested in an increase in body size, stimulation of skeletal growth, activation of anabolic processes, in particular protein biosynthesis, in fat-mobilizing action and other effects. Growth hormone not only stimulates, but regulates and coordinates metabolic processes, depending on the changing needs of the body. Secretion of hypertension by the pituitary gland sharply increases under various extreme situations and changes especially strongly with a decrease in blood sugar.

 Recently, certain information has been obtained not only about the sequence of amino acids in growth hormone, but also about the spatial structure of the molecule. A study of the dispersion of optical rotation and circular dichroism spectra shows that 55% of the growth hormone peptide chain has an α-helix structure. In human hypertension, a rather stable tertiary structure is found, which is completely preserved after the restoration and alkylation of two disulfide bridges.

 The study of the primary structure of SG in animals and humans is of significant interest, since SG has species specificity and SG of animal origin is inactive in humans. Therefore, receptors that perceive the effect of hypertension in humans differ from animal receptors and corresponding differences exist in the structure of hormones. The primary structure of human growth hormone and sheep is distinguished by the location of 70 amino acid residues. These differences are significantly larger than those observed in SH sheep and bovine. They may well explain the fact that growth hormone of animal origin is inactive in humans. At the same time, human and sheep SG has approximately the same activity in rats.

 Studies by foreign scientists have shown that enzyme-modified growth hormone, in which there is no fragment of the peptide chain 135-146, has high growth and lactogenic activity, which is several times greater than the activity of the intact hormone.

 These data allow us to think that protein hormones, like fragments, can be activated in the body, and their biological effect is carried out by a special activated form of the hormone [2].

 Our studies have shown that SG can be activated in another way. In particular, we held the SG preparation for 3-6 hours in the dark in the cold and received a drug with wider capabilities. We proceeded from the following reasoning.

 A wide range of previous studies showed that SG is one of the most important hormones that is responsible for renewal, and therefore for cell rejuvenation. Its maximum level in the blood is observed in humans during the period of embryonic development - at this time its content is approximately 40 times higher than that in adults.

 SG starts a number of processes that, firstly, suppress destructive processes in the body (inhibit the action of free radicals, etc.), and, secondly, a number of processes that promote cell renewal. SG increases the rate at which amino acids enter the cell. It also stimulates the synthesis of RNA, with the help of which new proteins are assembled instead of worn ones. All this leads to an increase in total protein synthesis and cell division. Moreover, building material and energy for the construction of new proteins and tissues of the SG takes from the body's fat reserves. Due to this, the use of the hormone leads to an increase in muscle and a decrease in body fat mass.

 SG causes an increase in protein synthesis in the body. Strengthening protein synthesis is achieved by enhancing the synthesis of DNA, RNA and increasing the rate of incorporation of amino acids into matrix synthesis. With the use of hypertension, muscle growth and bone thickening are observed. Strengthening of protein synthesis also occurs in the heart, liver, and kidneys, which positively affects their work. In addition to the proteanabolic effect, somatotropin enhances the intake of sodium, magnesium, potassium, sulfur, phosphorus, etc. The introduction of SG is accompanied by an increase in the oxidation of fats in the body with a general decrease in the content of adipose tissue and cholesterol [3].

 The main side effect of the drug is a diabetic effect. The drug can cause the development of diabetes in people who have a hereditary predisposition to it. Therefore, treatment with growth hormone should be carried out under the strict control of blood sugar. The diabetic effect of SG has a complex nature and depends mainly on the following points: 1) Switching of insulin from carbohydrate to protein metabolism. 2) Increased breakdown of insulin in the liver by the action of the enzyme insulinase. 3) Decreased absorption of glucose by tissues with a one-day increase in the absorption of amino acids by them.

 To reduce the side effect of SG-diabetogenic effect, we carried out a special effect on the preparation of SG, which we designated as "activation of SG" with the preparation of the drug, which is conditionally called activated somatotropic hormone (ASH), i.e. biogenic stimulant from a protein preparation.

Activation was carried out by keeping the SG in the dark at t ^o = 1-5 ^o C for 3-6 hours. The drug can be administered per se or in combination with other bioactive substances. It is intended for use in adult patients for the correction of various pathological as well as uncomfortable conditions, as well as in gerontological practice.

 The expansion and enhancement of the spectrum of action of activated somatotropin (ACT) occurs presumably due to modification of the spatial structure of the protein, consisting of a sufficiently long amino acid sequence.

To do this, the SG-containing preparation (natural or synthetic) is dissolved in water for injection, cooled to 2 ^o C, for example, directly in an ampoule or sterile dishes, then the solution is placed in sterile conditions and incubated in dark conditions at a temperature of 1-5 ^o C within 3-6 hours.

 In this case, the molecular mass does not change, and the change in activity occurs, most likely, due to the modification of the spatial configuration of the protein molecule.

 The table below shows the data characterizing some of the physicochemical properties of the initial (STH) and activated drug (ASH).

 The absence of disulfide bridges in the structure connecting the terminal phenylalanine residues with cysteine residues in the central region of the hormone molecule indicates a change in its configuration after activation.

 Experimental studies in laboratory animals, as well as some studies with the participation of a voluntary contingent of patients, have confirmed the presence of a number of positive properties in the ACH preparation, which were not found in the SG or showed very little.

 To illustrate the solution of the technical problem, the effect of ASH was checked for various pathologies and for some uncomfortable or physiologically stressed states of the body in order to identify the therapeutic and healing effects of our BS.

 The invention is illustrated by the following examples.

 EXAMPLE 1. An experimental study of the action of ASH on physical performance, endurance to adapt the physical condition of the body to intense physical activity (for example, the dynamics of biogenic amines and reactive properties of blood).

 For research, white laboratory rats of the Wistar strain were used. Studies of reactive blood indices, the effect of ASH and STH in a dose of 0.05 U per animal on the bioamine structures of the thymus and spleen by the luminescent method (the luminescence intensity was measured in some histological structures) under the influence of physical activity in dynamics (from 1 to 14 days) were studied. The results are shown in FIG. 1 (in% of control, i.e., without the introduction of any drug). Catecholamines (CA) and serotonin (ST) were studied by luminescence.

 According to the results of FIG. 1, it is seen that administration of STH to intact rats (without physical activity) leads to a decrease in CA and an increase in CT by 8-10 days and a return to normal by 13 days. Physical activity (running) exacerbates the picture, we see a sharp decrease in CA in the very first days after the introduction of STH and a simultaneous increase in ST, at the same time this effect, but with the introduction of ASG does not cause a strong deviation of the CA and ST, while the return of these indicators to normal occurs when exposed to ASH earlier (on day 12) than when STH is introduced (on day 14). These studies confirm the strengthening of adaptive-compensatory mechanisms with the introduction of ASH during exercise.

 FIG. 2 reflects the results of studies of myogenic leukocytosis in the contingent of volunteers involved in athletics. The results are also presented in% of control (measurement of leukocytosis during exercise without drug administration) compared with volunteers who were administered ASH and SG. Figure 2 presents the dynamics of changes in the leukocyte phase with the introduction of ASH, STH and in control. It is seen that the introduction of ASH in the shortest possible time restores the normal functional state of the body, thereby increasing the stamina of the body and restoring physical performance.

 EXAMPLE 2. The study of the dynamics of biogenic amines (BA) under the influence of stress and the influence of ASH on this dynamics.

 In the modern world, human life is constantly exposed to environmental stresses that cause changes in the level of biogenic amines, in particular, in the red bone marrow. We have studied the following BA: CA, serotonin by the method of luminescent histochemistry. The results are shown in FIG. 3. The experiments were performed on white laboratory rats. The result is presented in% of control, i.e. level of CT and CA during standard experiments on the stress response of rats without drug administration.

 Figure 3 shows that in the control the stressful situation increased the level of CT and CA in the first day. The introduction of STH created a protective system, which affected the later and lower levels of both BA. The best results in protecting against stress were given by the administration of ASH.

 EXAMPLE 3. The study of the dynamics of asthma under the influence of ASH on the background of the body load in the short-wave range of low-frequency electromagnetic radiation (EMR).

 The urbanization of modern life in all areas leads to harmful effects on the body from all sides: at work, on the street, in everyday life, the action of such an important component as electromagnetic cannot be ignored today with the ever-increasing number of computers, TVs, radiotelephones surrounding us, EHF ovens, etc.

 We conducted studies on the effect of ASH and STH on the dynamics of AD (serotonin and catecholamines) in the red bone marrow of white laboratory rats.

 The results are presented in figure 4 (in% relative to control). As a control, a group of rats exposed to EHF radiation without exposure to test drugs was used. Irradiation was carried out on the first day, then the level of CT and KA was determined in dynamics from 1 to 16 days using the luminescent method, the test drugs were also administered on the first day of EHF irradiation.

 From FIG. Figure 4 shows that ASH provides the most significant protection for the body when exposed to EHF.

 EXAMPLE 4. The effect of ASH in comparison with STH on the cellular composition of the spleen in an experimental bone fracture (tibia in mice under nembutal anesthesia) was studied. The observation was carried out for 2 weeks. Figure 5 shows the results of a study of the system of macrophages (MS) and blast cells (CD). It can be seen from the graph that the administration of ASH during an experimental fracture (0.05 U per mouse) had the most optimal immunomodulating effect (p <0.001) on the body during a bone fracture by the example of stimulation of the immune system of the spleen of a laboratory animal.

 EXAMPLE 5. Overweight, obesity of varying degrees, an increase in blood cholesterol is also an urgent problem today.

Recent studies show that a very important role is played by the ratio of the levels of high density lipids (HDL) and low density lipids (LDL). Tests on the contingent of volunteer patients who were injected with our ASH preparation showed that, in a comparative aspect, with the introduction of STH, the ASH preparation gives a 10-15% greater result than STH administration for the same period (28-30 days) (p <0 , 01)
EXAMPLE 6. Environmental degradation, deforestation, concentration of exhaust gases and smok in the air, the harmful effects of a number of other xenobiotic influences leads to disruption of normal metabolic processes in the cell, often to the destruction of structural elements of both the cell and tissues living organism. All this leads to accelerated aging of the body, gives rise to gerontological problems at an early age. Studies of lipid peroxidation (lipid peroxidation) allow in modern conditions to monitor and identify destructive processes in cell membranes [3].

 We have studied the level of malondialdehyde (MD) in the blood plasma of volunteer patients living in environmentally disadvantaged areas (in areas heavily contaminated with chemical emissions). Under the action of cytotoxic substances, the body's antioxidant system is not able to neutralize LPO activation. The introduction of our drug ASH in a daily dose of 0.5-1.0 Units led to a decrease in blood pressure by 20-25% for 2-3 weeks in patients compared with those who did not receive ASH.

 EXAMPLE 7. Studies on volunteer patients with coronary heart disease (coronary heart disease). We examined 21 patients (men aged 45-65 years), 7 healthy men aged 45-55 years as a control group. Of 21 patients, 10 patients received ASH in a daily dose of 0.5-1.0 units 2 times a week for 4 weeks. 11 patients received STH in the same dose. All patients with coronary artery disease received basic therapy. The following rheological properties of blood were measured: hematocrit, platelet aggregation, thrombin time, blood viscosity, cholesterol and fibrinogen levels. The comparison was carried out in dynamics over 28 days according to the control with healthy patients. It was found that in patients who did not receive ASH, fluctuations in rheological indices were observed throughout the entire period, while in patients who received ASH, by 20-26 days, these indices were almost normal (p <0.01).

 EXAMPLE 8. The dynamics of the quantitative indicators of peripheral blood cells and their correlation with the healing time after a mechanical injury of the musculoskeletal system was investigated. The experiments were carried out on white outbred rats. It was found that the administration of the ASH drug at a dose of 0.03 U per animal per day significantly - by 17-20% (p <0.001) accelerated the healing of muscle and skin wounds in comparison with animals that did not receive the drug. The picture of a general analysis of peripheral blood confirmed the result.

EXAMPLE 9. Study of the effect of ASH on the course of thrombocytosis in rheumatoid arthritis. Rheumatoid arthritis is closely associated with systemic damage to the connective tissue and in the acute stage is accompanied by thrombocytosis by 30-120% in comparison with remission. In the acute stage, a significant (p <0.001) increase in the number of macro-platelet forms (by light-optical microscopy) was detected. 19 voluntary patients took part in our experiments - 9 healthy men aged 42 to 56 years and 10 sick patients aged 45 to 60 years. Sick patients (7 out of 10) were administered ASH at a dose of 0.5-1.0 U per day (for 14 days, every other day). On days 6–7, thrombocytosis decreased by 35–50%, by day 14, the platelet level approached normal, while patients who did not receive ASH had a high thrombocytosis percentage of 17–20% on days 13–14 (p < 0.01)
EXAMPLE 10. The study of the effect of ASH on the immune system in a chronic inflammatory process with an immunodeficiency state. Patient volunteers with peritonitis were studied. The level of T-cell immunity was measured by the ratio of T-helper cells (CD4) to the level of T-killer cells (CD8). Injection of ASH of 1.5 U per day every other day for 7-8 days improved the ratio of CD4 / CD8 in favor of T-helper cells. The presented examples characterize the drug as a powerful multifunctional biogenic stimulator, which can be used with great effect in many pathologies, as well as for practically healthy patients who need to correct the functional state of various organs and systems.

 Another aspect of the invention is a method of biogenic stimulation, which consists in the introduction of an ASH preparation into an adult body in need of correction of a condition. The drug is administered parenterally, mainly intramuscularly, in a dose effective to obtain the desired result.

 The introduction of the drug can be carried out in combination with other biologically active substances to enhance and / or stabilize the desired result. The drug is best administered in discrete courses, for example, for 3-4 weeks with breaks of 8-10 weeks. The optimal daily dose of 1.5-3.0 units after 2, 3 days for 3-4 weeks. Then take a break of 8-10 weeks, after which, if necessary, repeat the course. Contraindications to the use of ASH are oncological diseases.

 ASH can be used in combination with various bioactive substances, depending on the solution of tasks to correct the state of the body. Often in such cases, the daily dose of the drug should be reduced from 0.5-1.0 to 0.01-0.03 Units. It is possible to use even lower doses, depending on the individual condition of the patient and the compatibility of our drug with another used in a combined effect. In particular, ASH can be successfully used in conjunction with certain vitamin preparations, for example, calcium pantothenate, carnitine chloride, vitamin U etc. We have given a detailed description of some of them that are most suitable for sharing with ASH in the form of specific examples.

 EXAMPLE 11: Calcium pantothenate. Calcium salt of D - (+) - pantothenic acid. Calcium pantothenate (PC) significantly reduces basic metabolism, which leads to a rapid increase in total body weight, as a result of a decrease in the proportion of oxidized proteins. Lowers blood sugar, which contributes to the natural release of growth hormone. The synthesis of acetylcholine increases, increasing the tone of the parasipatic nervous system, which contributes to an increase in the strength of the neuromuscular system.

 PK enhances the synthesis of steroid hormones and hemoglobin. PK is a “cost-effective” drug because makes the body more economical. Significantly increases overall endurance and tolerance of loads. PC is involved in the most important reactions of energy transfer and phosphorus compounds. PC improves liver function and helps to eliminate toxins, alcohol, poisons, drugs from the body. Pantothenate has pronounced radioprotective properties, the release of radioactive substances from the body increases by 2 times. Per se it is used as a detoxification, anti-allergic, anti-inflammatory and general strengthening agent. It has a strong anti-stress effect. PC is recommended during maximum training loads and in the competitive period as an anti-stress agent, primarily in individuals with increased anxiety. The sedative (calming) effect of PK is enhanced when given in conjunction with vitamin U in equal amounts. The simultaneous administration of ASH in an amount effective for each patient enhances the anti-stress properties of PCs, especially during active training loads for athletes, for recreational activities for people who actively use mobile phones and other EHF equipment.

 EXAMPLE 12: Carnitine chloride (D, L-N- (1-carboxy-2-hydroxypropyl) -trimethylammonium chloride). Carnitine chloride (KX) reduces basal metabolic rate.

 KX promotes the penetration of fatty acids through the membranes of mitochondria. This mechanism underlies the increase in the stamina of the body. The ability of CH to “burn” adipose tissue is used to reduce excess weight. It is possible to use CH in conjunction with ASH in order to correct weight for individualization of the dose of ASH. A very significant effect is achieved by comparing the combined use of ASH and CH and their use separately. The combined use of ASH and CH is effective in chronic liver diseases, thyrotoxicosis, coronary heart disease, neurasthenia.

 EXAMPLE 13. Vitamin U (d, L-2-amino-4- (dimethylsulfonium) butyric acid chloride). Vitamin U is a derivative of methionine, an essential amino acid. Therefore, the drug can be considered not only as a vitamin, but also as a crystalline amino acid. Vitamin U improves digestion, normalizes the acid-forming function of the stomach: increased acidity decreases, and lowers increases. A valuable property of vitamin U is the presence of labile methyl groups that can easily be included in the exchange, due to which fat-mobilizing and lipolytic effects are achieved. Lowers blood cholesterol. Recently, there has been evidence of the effectiveness of vitamin U in endogenous depression (mood decline), which are not amenable to treatment with psychotropic drugs. Vitamin U can be recommended for joint use with ASH in order to relieve endogenous depression, normalize the discomfort of the gastrointestinal tract, and eliminate cellulite. In this case, a significant synergistic effect is observed.

 EXAMPLE 14 Vitamin K, Vikasol (2,3-dihydro-2-methyl-1,4naphthoquinone-2sulfonate sodium). Vitamin K is a naphthoquinone derivative. For a long time, vitamin K was used only as a means of increasing blood coagulation by enhancing the formation of prothrombin in the liver.

 In recent years, its additional effect has been discovered: enhancing protein synthesis in the liver and muscles, healing of stomach and duodenal ulcers and wound healing effect. Under the influence of vitamin K, collagen synthesis is enhanced, which gives strength to ligaments and skin. As a result of the use of vitamin K, the activity of pituitary eosinophilic cells secreting somatotropic hormone is increased, the simultaneous administration of small individual doses of ASH helps to strengthen and stabilize the positive properties of vitamin K. Vitamin K significantly improves bioenergy, improving oxidative phosphorylation, synthesis of ATP and creatine phosphate in the muscles. It is especially effective when used together with ASH for healing wounds, as well as ulcers of the gastrointestinal tract. Effectively the combined use of ASH and vitamin K for sagging muscles and skin.

 EXAMPLE 15: Nicotinic acid, vitamin PP (pyridinecarboxylic acid-3). Under the influence of nicotinic acid, the content of serotonin in the body increases, which is a mediator of the parasympathetic nervous system and increases the permeability of cells for amino acids and energy substrates. Niacin increases the acidity of the gastric juice and its digestibility, thereby improving the digestibility of food. At the same time, the rate of peristalsis of the gastrointestinal tract also increases and appetite increases.

 Nicotinic acid is usually prescribed in doses of 50 to 300 mg per day. The simultaneous administration of small individual doses of ASH helps to strengthen and stabilize the positive properties of vitamin K. Together with ASG with reduced body weight using individual doses of ASH. The course of treatment with nicotinic acid together with ASH can last up to 2-3 months, after which a break is needed.

 Contraindications for the use of nicotinic acid are peptic ulcer of the stomach and duodenal ulcer, increased acidity of gastric juice, and obesity of the liver.

The drug ASH can also be used in conjunction with coenzymes:
Coenzyme is a derivative of a vitamin, an active form in which vitamins are converted into the body. In some cases, the pharmacological effect of coenzyme introduced into the body from the outside is different from the pharmacological effect of vitamin.

 EXAMPLE 16: Flavinate (P- (riboflavin-5 ') - P2 (adenosine-5') disodium phosphate disodium). Flavinate, or flavin adenine nucleotide, forms enzymes that are involved in the synthesis of amino acids, lipids and carbohydrates. Flavinate improves the course of redox processes, normalizes cholesterol metabolism, enhances hemoglobin synthesis, and improves vision. Flavinate promotes the most complete breakdown of carbohydrates and increases the energy supply of the body during heavy physical work. In practice, flavinate is used in the treatment of dystrophic processes, diseases of the retina and glaucoma, in chronic diseases of the liver, pancreas and intestines, in some skin diseases, etc.

 Intramuscularly administered at 0.002 g once a day. The courses of treatment can last from 10 to 40 days. Breaks between courses of treatment for at least one month. Joint use with ASH helps to accelerate the healing of eye diseases, visual acuity. Accelerates recovery from skin lesions.

 EXAMPLE 17: Cobamamide. (Co- [a- (5,6-dimethylbenzimidazolyl)] - Cob-adenazylcobamide). Cobamamide is a derivative of vitamin B12. The action of cobamamide is associated with increased cell division processes and is especially pronounced in relation to rapidly dividing cells, such as, for example, cells of the bone nucleus. The pharmacological effects of cobamamide are largely associated with the presence of labile methyl groups in its molecule that can participate in synthetic processes, enhance cholesterol breakdown and fat mobilization.

 The release form of cobamamide: ampoules of 0.5 and 1 mg of dry matter. Enter 1-1 times a day at 0.5-1 mg IM, previously dissolved in 1 ml of solvent.

 Joint use with ASH enhances the synergistic effect of cobamamide in bone fractures, accelerating the process of bone growth. At the same time helps to reduce fat mass.

 The drug ASH can very successfully be used in conjunction with vitamin-like substances.

 The term "vitamin-like substances" (VP) refers to compounds whose activity is manifested in small doses, comparable to doses of vitamins, but still significantly higher than the doses of the latter. All of them have relative harmlessness and low toxicity. Below are specific examples of the joint use of LHG with EP.

 EXAMPLE 18: Methyluracil (2,4-dioxo-6-methyl-1,2,3,4-tetrahydropyrimidine). Being a derivative of pyrimidine, methyluracil can serve as a source of source material for the synthesis of nucleic acids, thereby enhancing protein synthesis in the body.

 Methyluracil is prescribed mainly to accelerate the healing of wounds, ulcers, with chronic gastritis, as well as with a decrease in immunity. A characteristic feature of methyluracil is the ability to increase the content of leukocytes and erythrocytes in the blood, as well as have a slight anti-inflammatory effect in chronic inflammatory diseases.

 Methyluracil has some fat-mobilizing effect, under its influence, the content of fatty acids in the blood decreases. Perhaps this is due to the presence of a labile methyl group. The cosmetic effect of methyluracil is noteworthy. When using sufficiently large doses, the skin becomes juicy and supple. Daily doses from 2 to 9 g / day orally. The combined use of methyluracil with ASH gives a significant effect in reducing immunity, as well as in the healing of wounds, trophic ulcers, rejuvenation and increased skin elasticity.

 EXAMPLE 19: Potassium orotate (KO) (potassium salt of uracil-4-carboxylic (orotic) acid). Like methyluracil, potassium orotate is one of the precursors of the pyrimidine nucleotides that make up the nucleic acids that are involved in the synthesis of protein molecules. Unlike metalluracil, which is destroyed in the liver (only its individual fragments are included in the nucleotides), orotic acid is incorporated into the whole pyrimidine nucleotides. Indications for the appointment of potassium orotate are heart disease, increased physical activity, and chronic inflammatory diseases. Unlike methyluracil, which has a fat-mobilizing effect, potassium orotate, on the contrary, promotes the synthesis of fats and can cause liver obesity up to the development of its fatty degeneration (fatty degeneration of the liver with an excess of orotic acid can be prevented or reversed when vitamin E is added to the diet , choline, adenine), which must be considered when prescribing the drug.

 The combined use of KO and ASH, taking into account the addition of vitamin E, choline, adenine, etc., if necessary, is indicated for heart diseases, hypertension, chronic inflammatory diseases, for example, the respiratory system.

 Used daily doses of CO: from 0.5 to 1.5 g per day. Doses of simultaneous administration are selected individually taking into account the weight, as well as the pathology of the patient.

 EXAMPLE 20: Phosphaden (adenosine-5'-monophosphoric acid). Phosphaden is a fragment of adenosine triphosphoric acid - ATP. Phosphaden stimulates the synthesis of nucleotides, enhances redox processes, serves as an energy supplier for protein-synthetic processes in the body. The drug has a pronounced vasodilating effect, lowers blood pressure. Being a derivative of purine, phosphaden can serve as a starting material for the synthesis of nucleic acids. Phosphaden lowers blood lipids, fatty acids and b-lipoproteins. A feature of the drug is its pronounced therapeutic effect in relation to liver diseases, as well as the ability to improve energy metabolism of cells.

 Use tablets of 0.025 and 0.05 g or 2% injection. Daily doses of the drug when taken orally 0.1-0.2 g / day. In / m injected 2 ml of a 2% solution 2 times a day. The treatment courses take a long time. The courses are also very effective in conjunction with ASH in patients with coronary artery disease and severe hypertension.

 EXAMPLE 21: Riboxin (9-b-0-ribofuranosyl hypoxanthine). Like phosphadenine, riboxin is a derivative of purine and can be considered as a precursor of ATP. Unlike phosphaden, it does not have a phosphorus bond rich in energy, therefore it is less effective as an energy tool. It can be used for the same purpose and for the same indications as phosphaden.

 Daily doses for oral administration from 0.6 to 2.4 g. In the first days, take 0.6 g / day, then gradually increase the dose to 2 g / day. With the on / in the introduction of the first injected 10 ml per day, then the total amount of the drug, if necessary, adjusted to 20 ml 2 times a day. ASH injections are prescribed individually to enhance the effect of riboxin in heart diseases, arterial hypertension.

 EXAMPLE 22: Choline chloride (2-hydroxyethyl) -trimethylammonium chloride). Choline chloride (XX) is a precursor of acetylcholine and can serve as a starting material for its synthesis. Therefore, the introduction of choline chloride into the body leads to a sharp increase in the activity of cholinergic structures, which leads to an increase in neuromuscular conduction, an increase in muscle strength, an increase in intestinal motility, and anabolic processes in the body. The particular value of choline chloride is that it is part of the lecithin phospholipid, which lowers cholesterol in the body and prevents the development of atherosclerotic processes. Choline prevents and cures fatty degeneration of the liver of various etiologies due to the high content of labile methyl groups, improves kidney and thymus function. XX is involved in the construction of cell membranes and the formation of membranes of nerve trunks. XX improves memory, increases thinking productivity and learning ability. For these purposes, its joint use with ASH is very effective in the individual selection of doses of the latter.

 Ampoules with 20% r-rom for iv administration are diluted to 1% r-ra. Inside, the drug is taken 5 ml (1 teaspoon) 3-5 times a day. Up to 300 ml of a 1% solution is administered intravenously. The course of treatment lasts from 7 days to a month. Effective in gerontological practice.

 Nootropic drugs are a whole group of compounds used to improve the processes of memory, attention, logical thinking, increase mental performance and strength of the main nervous processes. Some drugs from the group of nootropics have anabolic effects and increase physical performance. Our work allows us to recommend some nootropics in combination with ASH.

 EXAMPLE 23: Piracetam (nootropil) (2-oxo-1-pyrrolidinylacetamide). Piracetam was invented in Belgium in 1963. With this drug, the era of nootropics began, which exert their effect on the nervous system not by suppressing any reactions, but due to the total improvement of all metabolic and plastic processes in the central nervous system. Piracetam improves memory, mental performance, higher mental activity, concentration, etc. Piracetam enhances the synthesis of nucleic acids and proteins in the body, which ultimately leads to a powerful increase in anabolism not only in the cells of the nervous system, but also in skeletal muscle fibers, liver cells, etc. As a result of increased protein synthesis, the course of recovery and adaptive reactions of the body accelerates, and physical performance increases. The energy potential of cells increases due to increased ATP synthesis and their resistance to various adverse factors increases: intoxication, oxygen starvation, high temperatures, etc. The synthesis of phospholipids that are involved in the formation of cellular memoranes and normalize cholesterol metabolism is accelerated. Piracetam has a beneficial effect on the structure of mitochondria - the main energy subunits of the cell, which underlies a significant increase in endurance and aerobic performance.

 To restore neuropsychiatric activity and performance after traumatic brain injuries, strokes, intoxications, neuropsychiatric breakdowns and depression, the drug is especially effective when combined with injections of ASH, this combination is very useful in gerontological practice.

 The course of treatment can last from several days to a year and depends on the patient's condition.

 EXAMPLE 24: Pantogam (calcium salt D - (+) - a, y, dioxi-b-b-dimethylbutyryl-aminobutyric acid). Pantogam (P) is a derivative of pantothenic and gamma-aminobutyric acids. The pharmacological properties of pantogam are a symbiosis of the effects of pantothenic and gamma-aminobutyric acids (gamma-aminobutyric acid is a inhibitory mediator of the central nervous system). Pantogam sharply lowers the main metabolism, causes a significant increase in body weight, increases the synthesis of acetylcholine and, as a result, muscle strength. It can also be recommended in combination with ASH for the same indications as for nootropil.

 The daily dose is 1.5-3 g. The course of treatment is from 1 to 6 months.

 Psycho-energizers are a relatively new group of drugs. All drugs in this group are able to increase the strength and mobility of nervous processes, mental and physical performance. In Russia, only one drug of this group is produced: acefen.

 EXAMPLE 25: Acefen (centrophenoxin) (b-dimethylaminoethyl ester of p-chlorophenoxyacetic acid hydrochloride). Acefen was discovered in the development of plant growth stimulants. The anabolic and psychostimulating effect of acefen is based on its ability to increase the content of choline in the brain and peripheral nerve structures, which leads to increased activity of cholinergic structures. At the same time, the speed of the nerve impulse along the nerve trunks increases, and the synthesis of acetylcholine is enhanced. Acefen is extremely beneficial for lipid metabolism, enhancing the synthesis of brain phospholipids, membranes of nerve cells and the liver. This mechanism underlies a significant improvement in memory with the use of acefen. Acefen reduces the content of lipofuscin in the brain cells, which is an “aging pigment”, thereby exerting a “rejuvenating” effect on the central nervous system. In gerontological practice, to improve memory, as well as to "rejuvenate" the body is very effective in combination with injections of ASH.

 Acefen is prescribed intramuscularly at 0.25 g per injection 1-3 times a day. Acefen treatment courses can last up to 3 months or more in conjunction with ASH. Side effects, as a rule, do not happen.

 Antihypoxants - a class of compounds that increase the body's resistance to oxygen deficiency. Joint use with ASH can enhance the positive activity of antihypoxants.

 EXAMPLE 26: Sodium hydroxybutyrate (sodium salt of y-hydroxybutyric acid). Sodium oxybutyrate (OK) is an exceptionally strong antihypoxant that protects the body from oxygen starvation in a rarefied atmosphere, with great physical exertion, with severe vascular diseases and damage to the respiratory apparatus. The antihypoxic properties of oxybutyrate are associated with its ability to activate the oxygen-free oxidation of energy substrates and reduce the body's need for oxygen. In addition, sodium oxybutyrate itself is able to break down with the formation of energy stored in the form of ATP. Due to all these properties, sodium oxybutyrate is by far the most effective tool for developing endurance.

 Under the influence of oxybutyrate, hypertrophy of mitochondria and muscle fibers occurs, the amount of glycogen in the muscles and in the liver increases. Co-administration of 0.01-0.03 U of ASH every other day enhances the positive effect of OK to normalize sleep.

 EXAMPLE 27: Glutamic acid (HA). 2-Aminoglutaric acid. Glutamic acid is a replaceable amino acid and takes a very important part in nitrogen metabolism, because most of the essential amino acids pass through metabolic reactions through the stage of conversion to glutamic and aspartic acids. In other words, HA is an important source material for amino acid synthesis in the body. Glutamic acid neutralizes ammonia, which in combination with HA forms glutamine, which is used for synthetic processes. HA stimulates oxidative processes, improves protein and carbohydrate metabolism, and improves the energy supply of brain functions. HA contributes to an increase in the level of endogenous aminobutyric acid. The introduction of HA reduces the accumulation of lactic acid in the blood, eliminating post-loading acidosis and increasing endurance. GK plays the role of a neurotransmitter in the spinal cord, facilitating the transmission of nervous excitation in synapses. HA promotes the synthesis of acetylcholine and ATP, as well as the transfer of potassium ions through cell membranes, which enhances muscle contraction processes. The introduction of HA improves higher nervous activity, improves mood and activity.

 To correct these conditions with stabilization of the effects obtained, it is very effective in combination with ASH, which in this case is used in an individual dosage.

 EXAMPLE 28: Aspartic acid. Potassium asparaginate + magnesium asparaginate. A combined preparation “Panangin” is made, each tablet of which contains 0.158 g of potassium asparaginate and 0.14 g of magnesium asparaginate. A similar drug called Asparkam contains 0.175 potassium and magnesium asparaginate. Panangin is also available in ampoules of 10 ml. Aspartic acid is actively involved in amino acid metabolism, being the starting material for the synthesis of essential amino acids in the body. Asparaginate increases the permeability of cell membranes for potassium and magnesium, which increases the activity of synthetic processes in cells and facilitates the process of muscle contraction. In the experiment, a mixture of potassium and magnesium salts of aspartic acid significantly increases overall endurance and activates anabolic processes in the muscles.

 Panangin and aspartame are used in conjunction with ASH to normalize cardiac activity in an individual dose.

 EXAMPLE 29: Histidine. L-p-Imidazolylalanine. Histidine is an essential amino acid. When introduced into the body causes a significant increase in the secretion of growth hormone. Histidine is actively involved in the synthesis of carnosine - a nitrogenous extractive substance of muscles, improves nitrogen balance. Histidine improves liver function, increases gastric secretion and intestinal motor activity. Histidine increases immunity and weakens the effect on the body of extreme factors, normalizes heart rate. Used for peptic ulcer, gastritis, hepatitis, decreased immunity and atherosclerosis. To obtain a stable immunogenic effect and correct the state of the body, ASG is used in conjunction with injections of ASH in an individual dosage of 0.01 Units per day.

 Histidine release form: 4% solution of histidine hydrochloride in ampoules of 5 ml for i / m administration. Assign 5 ml every other day for 30 days, alternating with ASH.

EXAMPLE 30: Methionine. D, Lb-amino-y-methylthiobutyric acid. Methionine is an essential amino acid. Being the owner of a highly mobile methyl group (-CH ₃ ), methionine takes part in the synthesis of choline and phospholipids, participates in the formation and metabolism of sulfur-containing amino acids, and stimulates the release of growth hormone. Methionine helps maintain nitrogen balance in the body, enhances the synthesis of steroid hormones, protects adrenaline from oxidation, and neutralizes many toxic products. In gerontological practice, for general healing and "rejuvenation" of the body, it is effective when used together with ASH at an individual dosage of the latter.

 When introduced into the body, methionine reduces the amount of neutral fat in the liver and lowers cholesterol in the blood, is used for diseases of the liver and pancreas, for protein deficiency and dystrophy.

 Herbal preparations, which increase their performance by their properties, can surpass many synthetic preparations. At the same time, they practically do not have toxicity, are well tolerated, and have very few contraindications. They can be used both independently and with nutrients to mutually potentiate their action. This is achieved by increasing the activity of the synthesis of cAMP, cGMP and other mediators that increase the sensitivity of cells to the body's own hormones. cAMP, for example, increases the sensitivity of target cells to the action of endogenous growth hormone and insulin, enhancing the effects of the latter. All RA can be divided into two large groups: RA adaptogens and RA of hypoglycemic action.

 RA adaptogens are so named because, in addition to anabolic effects, they have the ability to increase the body's resistance to a variety of adverse factors: physical exertion, hypoxia, toxins, radioactive and electromagnetic radiation, etc.

 EXAMPLE 31: Leuzea safflower (maral root). The plant grows in the mountains of Altai, in Western and Eastern Siberia, in Central Asia. Leuzea contains phytoecdysones - polyhydroxylated steroid compounds with pronounced anabolic activity. The introduction of leuzea extract into the body enhances protein-synthetic processes, promotes the accumulation of protein in the muscles, liver, heart and kidneys. Significantly increased physical stamina and mental performance.

 A distinctive feature of leuzea is the ability to improve the composition of peripheral blood by enhancing mitotic activity in bone marrow cells. In the blood, the content of red blood cells, white blood cells, hemoglobin increases. Immunity increases. Leuzea comes in the form of an alcoholic extract from rhizomes with the roots of Leuzea in 40 ml vials. Take 1 time a day in the morning at a dose of 20 drops to 1 teaspoon. The selected individual dosage of ASH, used together, enhances and stabilizes the effect of taking levzea.

 EXAMPLE 32: Rhodiola rosea (Golden root). Rhodiola rosea grows in Altai, Sayan, in Eastern Siberia and the Far East. The pharmacological effects of the golden root are due to the presence of substances such as rhodosin and rhodioloside. In some countries, they are produced in pure form. A distinctive feature of the golden root is the most powerful action in relation to muscle tissue. Increases muscle strength and strength endurance. The activity of contractile proteins of actin and myosin increases. The size of mitochondria increases. Effectively combined with the introduction of ASH.

 EXAMPLE 33: Aralia Manchurian. A distinctive feature of aralia is the ability to cause quite noticeable hypoglycemia (a decrease in blood sugar), which exceeds the hypoglycemia caused by other RA adaptogens. Since hypoglycemia in this case is accompanied by the release of growth hormone, taking Manchurian aralia causes a significant general anabolic effect with a strong increase in appetite and weight gain. The pharmacological effects of aralia are due to the presence of a special kind of glycosides-aralosides A, B, C, etc.

 To increase efficiency, it is very effectively combined with the introduction of ASH.

 EXAMPLE 34: Eleutherococcus prickly. Prickly Eleutherococcus contains the sum of glycosides - eleutherosides. Eleutherosides increase efficiency and enhance protein synthesis. Carbohydrate synthesis is also increasing. Fat synthesis is inhibited. The oxidation of fatty acids during physical work is enhanced. Eleutherococcus is characterized by its ability to improve color vision and liver function. Eleutherococcus is produced in the form of an alcohol extract from rhizomes with roots of 50 ml. Take from 10 drops to 1 teaspoon 1 time per day in the morning. The effect is enhanced and fixed in the combined administration of ASH in an individual dosage.

 The following are other herbal preparations that are effective in combination with ASH taking into account the daily rhythm of RA. It is most advisable to take them in the morning in combination with an injection of ASH.

 Schisandra chinensis. Distributed in the Primorsky and Khabarovsk Territories. The main pharmacological effects of lemongrass are due to the content of a crystalline substance, schizandrin. The characteristic features of lemongrass are a significant increase in working capacity, an improvement in mood, and an increase in visual acuity. All these effects are due to the ability of lemongrass to improve nerve conduction, nerve cell sensitivity and enhance excitation processes in the central nervous system. Release form: alcohol tincture of 50 ml in bottles. Take 10-25 drops 1 time per day (in the morning). Combined with ASH in an individual dosage.

 Zamaniha high. Grows in the Far East. Contains saponins, alkaloids and glycosides. It has a tonic and mild anabolic effect. According to the effectiveness of the general strengthening action, it is similar to ginseng. Issued in the form of alcohol tincture of 50 ml. Take 30-60 drops 1 time per day. It is possible to administer ASH while taking the lure in individual doses.

 Sterculia is a platanifolia. Like eleutherococcus and ginseng, it stimulates performance and anabolic processes. Release form: alcohol tincture from plants in 25 ml bottles. Take 10-40 drops 1 time per day.

 In addition to RA, it is very effective to use some beekeeping products in combination with ASH injections to correct the condition of the body.

 Beekeeping products have moderate biogenic activity, they are practically harmless and have no contraindications, except in rare cases of allergies.

 EXAMPLE 35: Apilak (royal jelly). It is the secret of the uterine glands of working bees and serves as food for the larva of the future uterus. Since 1953, targeted research has been conducted on the properties of royal jelly in animal experiments, and since 1955 in humans. It was found that royal jelly is useful as a general strengthening agent for patients who are exhausted and weakened after serious illnesses, as well as in aging. In such patients, appetite appears, weight increases, they become peppy and cheerful.

 Apilak has anabolic, general tonic, anti-inflammatory, antispasmodic, bactericidal, antiviral effects. The anabolic effect of apilac is much stronger than the anabolic effect of methyluracil. Increased immunity, mental and physical performance. Apilac dilates the vessels of the heart, brain, etc. It has an exciting effect on the central nervous system. It reduces high blood pressure and increases low blood pressure. It improves mood, sometimes it causes euphoria. It enhances the synthesis of acetylcholine, which leads to an increase in muscle strength, and at the same time enhances the synthesis of adrenaline in the adrenal glands, which contributes to the development of endurance.

 Under the action of apilak, there is a significant increase in sexual function in men, which serves as the basis for its appointment in case of impotence. Gonadal stimulation is associated with exposure to some centers of the hypothalamus. Apilak has also proved to be quite good in the treatment of menopausal disorders in women. Royal jelly improves lipid metabolism, significantly reduces blood cholesterol.

 Apilak tablets are produced in Russia - a preparation from dried royal jelly. One tablet contains 10 mg of active substance. Take tablets strictly 1 time per day in the morning. Since apilac is destroyed in the stomach, it is taken under the tongue, where it is absorbed, bypassing the gastrointestinal tract. The dosage is strictly individual.

 For some, the tonic dose is 20 tablets taken in the morning under the tongue, and for others no more than 1 tablet. The positive effect of apilac stabilizes with the introduction of ASH. Recommended for improvement in gerontology and with a decrease in potency.

 EXAMPLE 36: Flower pollen. Flower pollen is a concentrate of male germ cells of flowering plants. Therefore, a specific property of pollen is the presence of sex hormone activity. Such hormone-like activity also determines the presence of a powerful anabolic effect in flower pollen. In addition, it is a concentrate of amino acids and hormone-like peptides. Pollen contains growth factors that have a regenerative effect. The value of flower pollen is that it does not addictive and side effects, can be used for a very long time. Pollen is recommended by a number of leading scientists in the world for the prevention of reduced working capacity and aging. The pollen diet restores lost strength and promotes detoxification. It is used for anemia, lethargy, weakness, emaciation, prostatitis, impotence. As a result of the use of flower pollen, the amount of glycogen in the liver and in skeletal muscles increases, and blood flow slightly increases.

 It is impossible to take pollen inside, because in the stomach it is destroyed by digestive juices, therefore it is taken only under the tongue, where it is absorbed into the blood, bypassing the gastrointestinal tract. In combination with microdoses, ASH (up to 0.001 units per day) can be used to stabilize positive results.

 ASH injections are very effective in combination with the administration of actoprotectors. Actoprotectors (A) is a whole group of synthetic drugs that prevent the development of fatigue and increase efficiency. The direct biogenic effect of actoprotectors on muscle tissue is weakly expressed, however, they have a strong indirect effect, since they can dramatically increase the load, which give a directly corrective effect. Under the action of actoprotectors, the glycogen content in the muscles, liver and heart increases. The efficiency of tissue respiration increases.

 In addition, by slightly lowering blood sugar levels, actoprotectors to some extent contribute to increased secretion of growth hormone. Actoprotectors improve memory, endurance, adaptation to a lack of oxygen, increase resistance to cold and heat. Actoprotectants are compounds of economizing action, contributing to the implementation of a certain amount of work with minimal cost. Actoprotectors reduce the rate of working breakdown of proteins, fats and carbohydrates. At the same time, the rate of synthesis of short-lived proteins responsible for the urgent adaptation of the body accelerates in the liver. In recent years, dozens of actoprotectors have been synthesized. All of them can be divided into 2 large groups: derivatives of guanylthiourea and derivatives of 2-mercantobenzimidazole.

 So far, only one drug from the group of 2-mercantobenzimidazole derivatives - bemitil - has entered into wide clinical practice.

 Bemitil is available in 100 mg tablets. 2 to 4 tablets per day are prescribed. Since the drug has the ability to accumulate in the body, it can be taken no more than 6 days in a row, after which they take a 3-day break, etc. Bemitil significantly increases overall stamina and body weight. Its action is stronger, the higher the physical activity. The increase in performance under the influence of boehmil can reach 200%, especially when working in conditions of oxygen deficiency. Bemitil increases resistance to high temperatures. The positive effects of boehmil are enhanced and stabilized for a long period (up to 4-6 months) when combined with ASH in micro doses (from 0.001 units per day).

 The invention allows to provide effective assistance to a wide range of patients in need of correction of the state of the body, both for pathology and for health and beauty purposes.

Literature
1. M.D. Mashkovsky "Medicines", 1986, v.2, pp. 173-176.

 2. "Biochemistry of hormones and hormonal regulation." / Ed. ON THE. Yudaev, Moscow, "Science", 1976, pp. 63-71. M. Kanungo "Biochemistry of aging", Moscow, 1986, pp. 174-189.

Claims (5)

 1. Biogenic stimulator of a multifunctional spectrum of activity, which is a growth hormone based on a natural, synthetic or recombinant drug, treated by incubation in the dark in the cold, for 3-6 hours to obtain activated somatotropic hormone (ASH).  2. The biogenic stimulator according to claim 1, characterized in that the initial preparation is somatotropin, norditropin, humatrop, genotropin, sizen.  3. The method of biogenic stimulation of the body, including the introduction into the body of a biologically active drug, characterized in that the biogenic stimulator of ASH is administered according to paragraphs. 1 and 2 in an effective amount.  4. The method according to p. 3. characterized in that the ASH is administered in combination with vitamin or vitamin-like drugs, herbal anabolics, as well as immunomodulators.  5. The method according to p. 4, characterized in that the ASH is administered in combination with vitamin or vitamin-like drugs selected from the group of calcium pantothenate, carnitine chloride, vitamin U, vitamin K, nicotinic acid, flavinate, cobamamide, methyluracil, potassium orotate, phosphaden , riboxin, choline chloride, piracetam, pantogam, acefen, sodium hydroxybutyrate, glutamic acid, aspartic acid, histidine, methionine; plant anabolics selected from the group of leuzea safflower, Rhodiola rosea, Aralia manchurian, Eleutherococcus prickly, Schisandra chinensis, high zamanicha, sterculia platanifolia, apilak, pollen, bemitil.

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