RU2207571C1 - Method for predicting disorders of menstrual function in young girls - Google Patents

Abstract

FIELD: medicine, gynecology. SUBSTANCE: due to infrared spectroscopy one should test saliva in the range of wave length being 1543-1425 cm^-1, determine average degree value for infrared radiation permeability at norm (from 47.8% to 56.3%) and its value within 38.7-47.7% one should predict disorders of menstrual function. Pronounced disorders are determined at 23.9- 38.6% value. EFFECT: higher information value, more simplified noninvasive method. 3 ex

Description

 The invention relates to medicine, namely to gynecology.

 The formation of a woman’s reproductive system is determined by her morphofunctional maturation in adolescence. Menstrual function in adolescent girls is an indicator of reproductive health. In this regard, the question of finding opportunities for predicting and early diagnosis of menstrual dysfunction at this stage of the development of the body becomes very relevant.

 As an analogue, we used the method of ultrasound examination of the uterus and ovaries using two methods: transabdominal and in addition to it transvaginal (in girls who have sex). Scanning was carried out on a Sonoace 4800 apparatus (South Korea) equipped with transabdominal (3.5 MHz) and transvaginal (6.5 MHz) sensors.

 The disadvantage of this method is the diagnosis is already expressed, i.e. organic pathology.

As a prototype, the authors propose using the determination of protein and steroid hormones (PRL, FSH, LH, E2, testosterone) of blood serum from the cubital vein by enzyme-linked immunosorbent assay (ELISA). The procedure for conducting ELISA of gonadotropic (protein) hormones was as follows: 20 μl of test serum, standard solutions and control serum were added to the tubes. 200 μl of conjugate and 1 bead coated with antibodies to FSH are added to each tube. Incubated for 30 minutes in an incubator with constant shaking. Using a washer, balls are washed from the reagents. Add 250 μl of the working solution of the substrate. Incubated in the dark with constant shaking for 15 minutes, after which 1 ml of sulfuric acid is added. Photometer at a wavelength of 450 nm using a Roche photometer. The method for determining steroid hormones (estradiol and testosterone) was carried out as follows: 25 μl (when determining estradiol) and 50 μl (when determining testosterone) of standard solutions, controls and test serum were added to the wells. 200 μl of conjugate are added and incubated for 120 minutes at 37 ^{° C.} without covering the plate with a film. The tablet is washed three times with washing buffer, carefully removing the buffer after each washing. Add 100 μl of the working solution of the substrate to each well. Incubated for 15 minutes at 25 ^o C, after which add 100 ál of stopping solution. Take readings using a tablet reader at a wavelength of 450 nm.

 The disadvantages of this method are its high cost, laboriousness, invasiveness, the need for multiple studies and insufficient informational content of the study due to the extreme instability of hormonal parameters in this age group.

 The authors propose the prediction of menstrual dysfunction according to the analysis of the average transmittance of infrared radiation from saliva, based on the study of the functional state of the body at the molecular level. To accomplish this task, the IKAR hardware-software system was used (Patent for invention 2137126 of 09/10/1999 "Method for the study of biological fluids and a device for its implementation"; Certificate of approval of the type of measuring instruments "IKAR-9/1 infrared analyzers" RU.C .010 5745 dated 11/20/1998).

The features of the infrared spectrum of the saliva of teenage girls were revealed by a 10-fold study of saliva by cyclic (cycle time of about 1 second) measurement of the degree of transmission of infrared radiation in nine sub-ranges: 1- (3500-3200 cm ^-1 ), 2- (3085- 2832 cm ^-1 ), 3- (2120-1880 cm ^-1 ), 4- (1710-1610 cm ^-1 ), 5- (1600-1535 cm ^-1 ), 6- (1543-1425 cm ^-1 ), 7- (1430-1210 cm ^-1 ), 8- (1127-1057 cm ^-1 ), 9- (1067-930 cm ^-1 ) and averaging the values obtained for each channel.

 The object of the study was the saliva of 114 adolescent girls, of which 84 girls had menstrual dysfunctions (main group), manifested by hypo-, hypermenstrual syndrome, irregular cycle and algomenorrhea, and 30 adolescent girls had normal menstrual function (control group).

It was found that the most informative for the diagnosis of menstrual dysfunction in adolescent girls is channel 6 with a wavelength range of 1543-1425 cm ^-1 , where the greatest differences in the degree of transmission of infrared radiation are found.

In accordance with the average degree of transmission of infrared radiation in the wavelength range of 1543-1425 cm ^-1 , teenage girls were divided into three groups. The first group with an average of 47.8 to 56.3% included girls with a normal menstrual cycle. They were characterized by the absence of functional and organic disorders in the formation of the reproductive system. The second group included patients with an infrared transmittance of 23.9-38.6%. For this contingent of patients, pronounced menstrual dysfunctions were diagnosed, characterized by the presence of not only functional, but also organic disorders in the formation of the reproductive system. The third group in terms of the degree of transmission of infrared radiation (38.7-47.7%) was approaching pathology, but clinically it was still characterized by normal indicators of menstrual function, and violations in the formation of the reproductive system were episodic, not clearly expressed.

Clinical example 1
Patient A., 15 years old. No complaints.

 Anamnesis: heredity is not burdened. Past diseases: chickenpox, mumps. Menstrual function from 12 years, 3-4 days, after 28 days. Denies sexuality. The last menstruation is 5 / V.

 Objective research: general condition is satisfactory. The skin is pink, clean. Satisfactory nutrition. Formula for sexual development: Ma3Pb3Ah3Me3. For organs without features. PS - 65 beats. per minute. HELL - 120/80 mm RT. Art. Physiological administration is normal.

 P.R .: pubic hair on the female type. The external genitalia are developed correctly. The hymen is not broken. The body of the uterus is dense, of normal size, painless, mobile, in the correct position. Appendages on both sides are not determined.

 An in-depth examination of hormonal parameters (serum FSH, LH, PRL, E2, TS) and ultrasound data of the uterus and ovaries corresponded to the age norm.

 The girl underwent IR spectroscopy of mixed saliva. According to the analysis of the average infrared transmittance of 54.3%, it was assigned to the group with normal indicators of menstrual function.

 At follow-up after 10 months, the patient did not complain, and her menstrual formula corresponded to normative indicators.

Clinical example 2
Patient A., 16 years old. No complaints.

 Anamnesis: heredity is not burdened. Past diseases: measles, scarlet fever, mumps. Menstrual function from 13 years, 3-4 days, after 30 days. Denies sexuality. The last menstruation is 15 / IV.

 Objective research: general condition is satisfactory. The skin is pink, clean. Low power. Formula for sexual development: Ma2Pb3Ax3Me3. For organs without features. PS - 75 beats. per minute. HELL - 110/70 mm RT. Art. Physiological administration is normal.

 P. R.: pubic hair on the female type. The external genitalia are developed correctly. The hymen is not broken. The body of the uterus is dense, of normal size, painless, mobile, in the correct position. Appendages on both sides are not determined.

 An in-depth examination of hormonal parameters (serum FSH, LH, PRL, E2, TS) and ultrasound data of the uterus and ovaries corresponded to the age norm.

 The girl underwent IR spectroscopy of mixed saliva. According to the analysis of the average transmittance of infrared radiation, corresponding to 41.2%, it was assigned to the risk group for the development of menstrual dysfunction.

 During the observation, after 10 months, the patient developed disorders of the type of opsomenorrhea, which allows the authors to talk about the possibility of prediction.

Clinical example 3
Patient S., 17 years old. Complains of a lack of menstrual function.

 Anamnesis: heredity is burdened with maternal hypertension. Past diseases: frequent acute respiratory viral infections, flu, tonsillitis. Menstrual function is absent. Denies sexuality. Objective research: general condition is satisfactory. The skin is pink, clean. Low power. Formula for sexual development: Ma2Pb3Ah3Me0. For organs without features. PS - 79 beats. per minute. HELL - 110/70 mm RT. Art. Physiological administration is normal.

 P. R.: pubic hair on the female type. The external genitalia are developed correctly. The hymen is not broken. The body of the uterus is dense, less than normal, painless, mobile, in the correct position. Appendages on both sides are not determined.

 During an in-depth examination according to ultrasound, hypoplasia of the uterus and ovaries was diagnosed, hormonal studies indicated a decrease in the level of estradiol and gonadotropins in the blood.

 The results of the analysis of the average transmittance of infrared radiation (in this case, equal to 24.1%) characterized the state of the girl’s homeostasis as altered and noted the fact of menstrual dysfunction as an indicator of the formation of the reproductive system.

Claims (1)

A method for predicting menstrual dysfunction in adolescent girls, characterized in that the method of infrared spectroscopy in the wavelength range equal to 1543-1425 cm ^-1 examine the saliva, determine the average transmittance of infrared radiation is normal (from 47.8 to 56, 3%) and when the average transmittance of infrared radiation from 38.7 to 47.7% is predicted, menstrual dysfunction is predicted, expressed violations are determined when the average transmittance is from 23.9 to 38.6%.