RU2176515C1 - Method of preparing total polysaccharide from vegetable raw - Google Patents

Abstract

FIELD: food industry, medicine. SUBSTANCE: raw is treated with formalin an aqueous solution to fix pigment followed by raw keeping in acidified water at pH 3.9-4.2 and temperature 48-51 C for 3-4 days. Then pectin polysaccharides are extracted with ammonium oxalate an aqueous solution at 68-70 C for 6-8 h, extract is filtered, concentrated on ultrafiltrating column with hole pores in combination with simultaneous dialysis and dried by lyophilic method. Freshly gathered plant fluorescences, for example, common tansy, Tanacetum vulgare L., containing pectin polysaccharides are used as raw source. EFFECT: improved method of preparing, increased yield of product. 3 cl, 1 tbl, 3 ex

Description

 The invention relates to the food industry and to medicine, namely to the pharmaceutical industry, and relates to a method for producing pectin polysaccharides.

Closest to the claimed is a method of obtaining polysaccharides from plant materials having an immunostimulating effect [RU patent 2149642, AKI A 61 K 35/78], which consists in extracting grass of common and duckweed grass after preliminary treatment with an aqueous formalin solution at a ratio of raw material: solution 1: 8 - 11 and keeping in acidified water at a pH of 3.8 - 4.2 for 3-4 hours and 45 - 52 ^o C at a ratio of raw materials: solution 1: 7 - 10, an aqueous solution of ammonium oxalate at a ratio of raw materials; solution 1: 8-12, respectively, for 3 to 8 hours and 65 to 70 ^° C, followed by evaporation, precipitation with 96% ethyl alcohol, filtration, dialysis and freeze drying.

However, this method is not effective enough for the isolation of polysaccharides from tansy, in addition, the disadvantage of this method is the need for evaporation in vacuum at a temperature of 40 - 45 ^o C, precipitation with 96% ethyl alcohol, followed by dialysis, which increases its time.

 The objective of the invention is to develop a new more economical and effective method for the isolation of pectin polysaccharides from plant materials, for example from tansy, to obtain the target product of high quality. The invention allows to isolate the amount of polysaccharides without the use of ethyl alcohol for precipitation.

 The technical result consists in obtaining the sum of pectin polysaccharides with a yield of the target product of 1.9 - 2.1% by weight of raw plant materials.

 The technical result is achieved by the fact that the extract is filtered, concentrated on an ultrafiltration column with hollow fibers with simultaneous dialysis, and freshly picked plant inflorescences containing pectin polysaccharides, for example, tansy, can be used as raw materials.

 The essential features of the invention.

 The method of obtaining from plant raw materials the sum of pectin polysaccharides, including processing the raw materials in an aqueous formalin solution to fix the pigment, keeping in acidified water at the appropriate pH and temperature to remove calcium and magnesium bridges, extracting polysaccharides with an aqueous solution of ammonium oxalate, after which the extract is filtered, concentrated on ultrafiltration column with hollow fibers with simultaneous dialysis and freeze-dried, while freshly collected can be used as raw material otsvetiya plants, e.g. tansy containing pectic polysaccharides.

The method was tested on the example of a higher plant - tansy, whose inflorescences are treated with a 0.5% aqueous formalin solution in the ratio of raw materials: 1: 9 - 13 solution for 16 - 18 hours to fix the pigment at a temperature of 21 - 25 ^o C. Before extraction, the raw materials are maintained, in addition, in water acidified with hydrochloric acid (pH 3.9 - 4.2; the ratio of raw materials: solution 1: 9-13) at 48 - 51 ^o C for 3-4 hours to remove calcium and magnesium bridges . The extraction of pectin polysaccharides is carried out with an aqueous 0.8 - 0.1% solution of ammonium oxalate in a ratio of 1: 9 - 13 at 68 - 70 ^o C for 6 - 8 hours. Next, the extract is filtered, concentrated on an ultrafiltration column with hollow fibers (UVA - 2 - 100 PA) with simultaneous dialysis and freeze-dried.

Example 1. 3.2 kg of freshly picked inflorescences of common tansy are poured with a 0.5% aqueous formalin solution (40 L) and left at room temperature for 18 hours. The darkened solution is drained, the raw materials are washed with water until colorless, squeezed and poured with water, acidified with hydrochloric acid (pH 4.0; 30 l). The mixture was kept at a temperature of 50 ^° C. for 4 hours. Then the solution was drained and the feed was washed with water. The polysaccharide is sequentially extracted with a 0.75% aqueous solution of ammonium oxalate (30 L), then with a 0.5% solution (30 L) and dist. water (60 l). The polysaccharide extraction is carried out at a temperature of 68 - 70 ^o C for 6 to 8 hours with regular stirring. The combined filtered extracts are concentrated on an ultrafiltration column with a hollow fiber (UVA - 2 - 100 PA) to 9 l and at the same time dialyzed by adding 55 l of dist. water. The purified polysaccharide solution is freeze-dried. The yield of 1.9% (table 1).

Example 2. 1.6 kg of freshly picked inflorescences of common tansy are poured with a 0.5% aqueous formalin solution (20 L) and left at room temperature for 16 hours. Further, the processing is carried out as described in example 1. The raw material is poured with acidified water (pH 4.0; 20 l). The mixture was kept at a temperature of 50 ^° C. for 4 hours. The polysaccharide was sequentially extracted with a 0.5% solution of ammonium oxalate (20 L), then with a 0.3% solution (20 L) and a 0.2% solution ( 10 l) under the same conditions. The combined filtered extracts are concentrated on an ultrafiltration column with a hollow fiber (UVA - 2 - 100 PA) to 6 l and at the same time dialyzed by adding 45 l of dist. water. The yield of 2.0%.

Example 3. 1.6 kg of freshly picked inflorescences of common tansy are poured with a 0.5% aqueous formalin solution (20 L) and left at room temperature for 16 hours. Further, the processing is carried out as described in example 1. The raw material is poured with acidified water (pH 4.0; 20 l). The mixture was kept at a temperature of 50 ^° C. for 3 hours. The polysaccharide was sequentially extracted with a 0.5% solution of ammonium oxalate (20 L), then with a 0.3% solution (20 L) and dist. water (10 l) under the same conditions. The combined filtered extracts are concentrated on an ultrafiltration column with a hollow fiber (UVA - 2 - 100 PA) to 6 l and at the same time dialyzed by adding 35 l of dist. water. The yield of 2.1%.

 The characteristics of the target products are given in the table.

 Thus, the present invention allows to isolate pectin polysaccharides from a common tansy plant with a significant reduction in time with a sufficiently high degree of purification of the target products. The method can be used for any higher plants containing pectin polysaccharides.

Claims (3)

1. The method of obtaining the amount of polysaccharides from plant materials containing pectin polysaccharides, including pre-treatment with an aqueous solution of formalin to fix the pigment, keeping in acidified water at pH 3.9-4.2 at 48-51 ^o C for 3-4 hours, extraction of pectic polysaccharides aqueous ammonium oxalate at 68-70 ^o C for 6-8 h, dialyzed and freeze-drying the desired product, characterized in that before the freeze dried extract was filtered and concentrated on an ultrafiltration column with simultaneous DIAL Zom.  2. The method according to claim 1, characterized in that the raw materials use freshly picked inflorescences of a plant, for example, tansy ordinary Tanacetum vulgare L.  3. The method according to claim 1, characterized in that for the concentration and dialysis of the extract using an ultrafiltration column with hollow fibers.

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