RU2112797C1 - Device for withdrawing top product phase from microorganism-growth apparatus - Google Patents

Abstract

FIELD: microbiological industry. SUBSTANCE: device comprises vertical telescopic tube installed coaxially with casing of the apparatus, discharge pipe, and means to create magnetic field in the upper part of culture broth and to isolate infecting microflora made in the form of vertical tubular framework with its top part communicating with telescopic tube. Framework-forming pipes have holes to discharge foam and liquid and are provided with wire gauzes to connect framework to constant current source and to produce negative charge in the top part of framework and positive charge in its bottom part. EFFECT: improved design. 1 dwg

Description

 The invention relates to the microbiological industry, more specifically to devices for removing the upper phase of the product from the apparatus for growing microorganisms.

 A device for removing the upper phase of the product (foam-liquid layer) from the apparatus for growing microorganisms, containing a vertically located pipe in communication with the area of the housing in which the upper layer of culture fluid is located, and an associated branch pipe connected to the selection apparatus [1 ].

 The disadvantage of this device is that it does not provide a clear separation of the phases of the culture fluid and does its job only when a certain level is reached in the apparatus.

 The closest technical solution to the proposed one is a device for draining the upper phase of the product from the apparatus for growing microorganisms, containing a telescopic tube vertically mounted on the axis of the apparatus body with a funnel in the upper part and an outlet pipe [2].

 However, during the operation of this device, the selection of the upper phase of the culture fluid is carried out not uniformly over its entire surface, but concentrated near the funnel, due to which this phase is not completely removed from the apparatus. In addition, during the operation of this device, there is no clear distinction between the phases of the culture fluid into the one that needs to be taken (removed) and the one that should remain in the apparatus.

 The technical result of the invention consists in a clearer separation of the phases of the culture fluid and timely removal of yeast and microorganisms infecting the culture fluid from the apparatus for growing microorganisms throughout the entire process and thus improving the quality of the biomass.

 This is achieved by the fact that the proposed device for draining the upper phase of the product from the apparatus for growing microorganisms, containing a telescopic tube and a branch pipe vertically mounted on the axis of the apparatus body, is equipped with a means for creating a magnetic field in the upper layer of the culture fluid and isolating the infectious microflora, made in the form vertically mounted tubular frame, the upper part of which is in communication with a telescopic discharge pipe, while the pipe frame has holes for water and foam and liquid are provided with fixed to them by wire grids for connection to a DC power source and generate a negative charge in the upper part of the frame and a positive charge in its lower part.

 The invention is illustrated in the drawing, which schematically shows the proposed device in a perspective view.

 A device for removing the upper phase of the product from the apparatus for growing microorganisms contains a telescopic tube 2 mounted along the axis of the body 1 of the apparatus with a branch pipe 3 and means for creating a magnetic field in the upper layer of the culture fluid and isolating the infectious microflora, made in the form of a vertically mounted tubular frame 4, the upper part 5 of which is communicated with a telescopic tube. The pipes of the upper part 5 of the frame have oval openings 6 for collecting foam on the lateral surfaces, and the pipes of the lower part 7 of the frame on the upper surface have slit-like openings 8 for collecting the liquid phase. The different shape of the holes is due to the different density of the selected phases. The tubes of the carcass are equipped with wire meshes 9 and 10 fixed to them to connect to a direct current source and create a negative charge in the upper part of the carcass and a positive charge in its lower part. The upper 5 and lower 7 parts of the frame 4 are rigidly interconnected using hollow racks 11.

 Communication telescopic pipe 2 with the upper part 5 of the frame provides the possibility of a vacuum in the pipes of the frame. The mass of the latter is calculated so that its upper part is above the surface of the liquid, and the lower one is immersed to the required depth.

 The device operates as follows.

 It can be installed both in continuous and periodic devices, since the presence of a telescopic pipe provides the ability to move in the housing 1 as a float.

 In most microbiological enterprises, if necessary, part of the culture fluid is taken during the process, it is carried out from the lower layers of the medium, thereby contributing to the removal of the largest cells of microorganisms from the apparatus. More rational is the selection of fluid from above. At the same time, foam, a significant part of the yeast and bacterial microflora, are involved in the flow of the selected fluid. It was also established that the concentration of dead cells in the foam phase is higher than in the liquid. Thus, during the selection of the upper phase of the culture fluid, biomass is continuously improved.

 The difference in the flotation ability of beneficial and wild yeast is known. Such differentiation allows one to selectively infect infectious microflora until a dynamic equilibrium is established with a low concentration of foreign cells in the population.

 Yeast cells always have a negative charge, and their recharge is impossible.

 Magnetic processing has a positive effect on yeast. The magnitude of the magnetic field and its duration are different for different cultures of microorganisms and must be determined in each case.

 On the wire mesh 9 and 10 serves DC voltage.

 Negatively charged yeast cells located between the grids will repel from the bottom 10 (negatively charged) and be attracted to the top 9 (positively charged). Yeast cells, which will be below the frame, will be repelled from it towards the bottom of the apparatus due to the homogeneity of the charges of the molecules and the lower wire mesh 10 of the source. After creating a magnetic field on the grids 9 and 10, which are fixed on the corresponding parts of the frame, the latter will be pushed out by the culture fluid to the phase separation level. As a result of this, in the housing 1 of the apparatus, the phases of the culture fluid are clearly separated into upper and lower.

 Simultaneously with applying voltage to the grids, a vacuum pump is switched on (not shown in the drawing). In the telescopic tube 2, a vacuum is created and the upper phase of the product begins to be removed from the housing through the openings 6 and 8 of the frame tubes.

 The level of magnetic field strength and the amount of the selected upper phase of the product are selected in a particular apparatus for growing microorganisms by changing the distance between the upper 5 and lower 7 parts of the frame, the size of the holes and the magnitude of the applied voltage.

 The proposed device can be used both for removal of the foam phase from the apparatus (during periodic cultivation of microorganisms), and for removal of the foam-liquid phase. Only the foam phase is selected by overlapping the slit-like openings 8 on the lower part of the frame.

 Thus, the design of the device provides a clear distinction between the upper and lower phases of the culture fluid and the timely removal of yeast and microorganisms infecting the culture fluid from the apparatus for growing microorganisms throughout the entire process, which improves the quality of the finished product.

Claims (1)

 A device for removing the upper phase of the product from the apparatus for growing microorganisms, comprising a telescopic tube and a branch pipe vertically mounted on the axis of the apparatus body, characterized in that it is provided with means for creating a magnetic field in the upper layer of the culture fluid and isolating the infectious microflora, made in the form of a vertically installed tubular frame, the upper part of which is connected with a telescopic discharge pipe, while the pipe frame has holes for the removal of foam and Liquids and provided with fixed to them by wire grids for connection to a DC power source and generate a negative charge in the upper part of the frame and a positive charge in its lower part.