CN212780197U

CN212780197U - Full-automatic polyacrylamide protein gel dyeing and decoloring instrument

Info

Publication number: CN212780197U
Application number: CN202021350284.4U
Authority: CN
Inventors: 孙鹏; 康雅明; 孙世平
Original assignee: Wuhan Bobaiou Biotechnology Co ltd
Current assignee: Wuhan Bobaiou Biotechnology Co ltd
Priority date: 2020-07-10
Filing date: 2020-07-10
Publication date: 2021-03-23
Anticipated expiration: 2030-07-10

Abstract

The utility model discloses a full-automatic polyacrylamide protein gel dyeing decoloration appearance, the on-line screen storage device comprises a base, the seat is placed to the top fixedly connected with of base, the cylinder appearance chamber has all been seted up to the both sides of placing a top portion, two the inside in cylinder appearance chamber all is provided with the activity and removes liquid mechanism, the activity removes liquid mechanism including collecting the lid, the surface of collecting the lid and the inner wall sliding connection that the cylinder held the chamber, the ring groove has been seted up at the top of collecting the lid, and the center department of collecting the caping portion runs through and has seted up the through-hole, collects the top fixedly connected with pull rod of lid, the utility model relates to an albumen glue processing technology. This full-automatic polyacrylamide protein gel dyeing decoloration appearance is convenient for hold the intracavity wall to the cylinder and scrape the material through setting up of pull ring, pull rod, collection lid, then the accessible ring groove is collected remaining the coloring agent, further through the cooperation of small-size pump, even pipe, U type pipe, is convenient for extract remaining the coloring agent, can not influence next dyeing decoloration work.

Description

Full-automatic polyacrylamide protein gel dyeing and decoloring instrument

Technical Field

The utility model relates to an albumen glue processing technology field specifically is a full-automatic polyacrylamide protein gel dyeing decoloration appearance.

Background

Polyacrylamide gel electrophoresis is a common electrophoresis technology which takes polyacrylamide gel as a supporting medium and is used for separating protein and oligonucleotide, and the polyacrylamide gel has a net structure and a molecular sieve effect. It has two forms: Native-PAGE (Native-PAGE) and denaturing polyacrylamide gel, wherein proteins can be kept in an intact state and are gradually separated in a gradient manner according to the molecular weight of the proteins, the shapes of the proteins and the charge quantity attached to the proteins; in the native polyacrylamide gel electrophoresis of DNA, the DNA migrates in a double-stranded state, and the mobility is influenced by the base composition and sequence.

Polyacrylamide protein gel electrophoresis is a common protein component analysis method in the biological field, the principle is simple, but the dyeing and the decoloring of the polyacrylamide protein gel are manually completed, the time is consumed, the existing dyeing and the decoloring of the polyacrylamide protein gel are generally carried out in a container, residual decoloring agents are easily adhered to the inner wall of the container after the decoloring work is completed, and the decoloring agents are not easily removed, so that the normal use of the device is influenced.

SUMMERY OF THE UTILITY MODEL

Not enough to prior art, the utility model provides a full-automatic polyacrylamide protein gel dyeing decoloration appearance has solved the inner wall and has easily adhered to remain the decolorant, and these decolorants are difficult to get rid of, influence the normal use of device and albumen glue dyeing decoloration and all need artifical manual completion, problem that expends time.

In order to achieve the above purpose, the utility model discloses a following technical scheme realizes: the utility model provides a full-automatic polyacrylamide protein gel dyeing decoloration appearance, includes the base, the seat is placed to the top fixedly connected with of base, the cylinder appearance chamber has all been seted up to the both sides of placing a top portion, two the cylinder holds the inside in chamber and all is provided with the activity and removes liquid mechanism.

The movable liquid removing mechanism comprises a collecting cover, the outer surface of the collecting cover is in sliding connection with the inner wall of a cylindrical containing cavity, a circular groove is formed in the top of the collecting cover, a through hole is formed in the center of the top of the collecting cover in a penetrating mode, a pull rod is fixedly connected to the top of the collecting cover, a placing seat is formed in the top end of the pull rod in a penetrating mode and extends to the outside of the placing seat, and a pull ring is fixedly connected to the top end of the pull rod.

Preferably, the collecting box is fixedly connected to the top of the base and behind the placing seat, the small-sized suction pump is fixedly connected to the top of the collecting box, and a liquid outlet of the small-sized suction pump is communicated with the top of the collecting box through a conduit.

Preferably, the liquid inlet of the small-sized suction pump is communicated with a connecting pipe, one end, far away from the small-sized suction pump, of the connecting pipe is communicated with a U-shaped pipe, and two ends of the U-shaped pipe penetrate through the two cylindrical cavities respectively and extend to the insides of the two cylindrical cavities.

Preferably, the both sides at base top all are provided with conveying mechanism, two conveying mechanism symmetry sets up, conveying mechanism is including placing the bottle, place the bottle and fix the top at the base, the top fixedly connected with delivery pump of base, the inlet intercommunication of delivery pump has first bellows, the one end that delivery pump was kept away from to first bellows runs through the inside of placing the bottle and extending to the bottle of placing.

Preferably, a liquid outlet of the conveying pump is communicated with a second flange, and one end, far away from the conveying pump, of the second flange penetrates through the placing seat and extends into the placing seat.

Preferably, four corners of the bottom of the base are fixedly connected with supporting rods, four supporting plates are fixedly connected between the surfaces of the supporting rods, two sides of the top of each supporting plate are fixedly connected with recovery boxes, the tops of the recovery boxes are communicated with straight pipes, two surfaces of the straight pipes are fixedly connected with electric control valves, and the top ends of the straight pipes sequentially penetrate through the base, the placing seat and the cylindrical accommodating cavity.

Advantageous effects

The utility model provides a full-automatic polyacrylamide protein gel dyeing decoloration appearance. Compared with the prior art, the method has the following beneficial effects:

(1) the full-automatic polyacrylamide protein gel dyeing and decoloring instrument is characterized in that a movable liquid removing mechanism is arranged inside two cylindrical cavities, the movable liquid removing mechanism comprises a collecting cover, the outer surface of the collecting cover is in sliding connection with the inner walls of the cylindrical cavities, the top of the collecting cover is provided with an annular groove, a through hole is formed in the center of the top of the collecting cover in a penetrating manner, the top of the collecting cover is fixedly connected with a pull rod, the top end of the pull rod penetrates through a placing seat and extends to the outside of the placing seat, the top end of the pull rod is fixedly connected with a pull ring, the top of a base is fixedly connected with a collecting box behind the placing seat, the top of the collecting box is fixedly connected with a small-sized suction pump, a liquid outlet of the small-sized suction pump is communicated with the top of the collecting box through a guide pipe, a connecting pipe is communicated with a liquid inlet of the small-sized suction pump, one end, away from the small-, the setting through pull ring, pull rod, collection lid is convenient for scrape the material to cylinder chamber inner wall, then accessible ring groove is collected remaining the decolorant, further through the cooperation of small-size pumper, connecting pipe, U type pipe, is convenient for extract remaining the decolorant for device ability normal use.

(2) The full-automatic polyacrylamide protein gel dyeing and decoloring instrument is characterized in that conveying mechanisms are arranged on two sides of the top of a base respectively, the two conveying mechanisms are symmetrically arranged, each conveying mechanism comprises a placing bottle fixed on the top of the base, a conveying pump is fixedly connected to the top of the base, a liquid inlet of the conveying pump is communicated with a first folding pipe, one end, far away from the conveying pump, of each first folding pipe penetrates through the placing bottle and extends to the inside of the placing bottle, a liquid outlet of the conveying pump is communicated with a second folding pipe, one end, far away from the conveying pump, of each second folding pipe penetrates through a placing seat and extends to the inside of the placing seat, the tops of two recovery boxes are communicated with straight pipes respectively, the surfaces of the two straight pipes are fixedly connected with electric control valves, the top ends of the straight pipes sequentially penetrate through the base, the placing seat is communicated with a cylindrical containing cavity, a coloring agent is directly, through the arrangement of the straight pipe, the electric control valve and the recovery box, the treated dyeing agent is convenient to recover, manual operation is not needed, and time is saved.

Drawings

FIG. 1 is a main sectional view of the structure of the present invention;

FIG. 2 is a front view of the movable liquid removing mechanism of the present invention;

FIG. 3 is a perspective view of the collecting cover structure of the present invention;

fig. 4 is a rear view of the local structure of the present invention.

In the figure: 1-base, 2-placing base, 3-cylindrical containing cavity, 4-movable liquid removing mechanism, 41-collecting cover, 42-circular groove, 43-through hole, 44-pull rod, 45-pull ring, 5-collecting box, 6-small pump, 7-conduit, 8-connecting pipe, 9-U-shaped pipe, 10-conveying mechanism, 101-placing bottle, 102-conveying pump, 103-first folding pipe, 104-second folding pipe, 11-support rod, 12-support plate, 13-recovery box, 14-straight pipe and 15-electric control valve.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Referring to fig. 1-4, the present invention provides a technical solution: a full-automatic polyacrylamide protein gel dyeing and decoloring instrument comprises a base 1, wherein the top of the base 1 is fixedly connected with a placing seat 2, the right side of the placing seat 2 is fixedly provided with a vibrating motor which has a vibrating effect and is electrically connected with an external power supply and is controlled by a control switch, two sides of the top of the placing seat 2 are respectively provided with a cylindrical accommodating cavity 3, the inner parts of the two cylindrical accommodating cavities 3 are respectively provided with a movable liquid removing mechanism 4, the movable liquid removing mechanism 4 comprises a collecting cover 41, the outer surface of the collecting cover 41 is in sliding connection with the inner wall of the cylindrical accommodating cavity 3, the top of the collecting cover 41 is provided with an annular groove 42 which is convenient for collecting residual decoloring agent, the center of the top of the collecting cover 41 is provided with a through hole 43 in a penetrating way so that the coloring agent can flow down, the top of the collecting cover 41 is fixedly connected with a pull rod 44, the top end of the pull rod 44 penetrates, the collecting box 5 is fixedly connected with the top of the base 1 and positioned behind the placing seat 2, the small-sized suction pump 6 is fixedly connected with the top of the collecting box 5, the collecting box is electrically connected with an external power supply and is controlled by a control switch, a liquid outlet of the small-sized suction pump 6 is communicated with the top of the collecting box 5 through a conduit 7, a liquid inlet of the small-sized suction pump 6 is communicated with a connecting pipe 8, one end of the connecting pipe 8, which is far away from the small-sized suction pump 6, is communicated with a U-shaped pipe 9, two ends of the U-shaped pipe 9 are positioned right above the two annular grooves 42, two ends of the U-shaped pipe 9 respectively penetrate through the two cylindrical containing cavities 3 and extend to the insides of the two cylindrical containing cavities 3, conveying mechanisms 10 are arranged on two sides of the top of the base 1, the two conveying mechanisms 10 are symmetrically arranged, each conveying mechanism 10 comprises a placing bottle 101, the device is electrically connected with an external power supply and is controlled by a control switch, a liquid inlet of the delivery pump 102 is communicated with a first folding pipe 103, one end of the first folding pipe 103, far away from the delivery pump 102, penetrates through the placing bottle 101 and extends into the placing bottle 101, a liquid outlet of the delivery pump 102 is communicated with a second folding pipe 104, one end of the second folding pipe 104, far away from the delivery pump 102, penetrates through the placing seat 2 and extends into the placing seat 2, four corners of the bottom of the base 1 are fixedly connected with supporting rods 11, supporting plates 12 are fixedly connected between the surfaces of the four supporting rods 11, two sides of the top of each supporting plate 12 are fixedly connected with recovery boxes 13, the tops of the two recovery boxes 13 are communicated with straight pipes 14, and the surfaces of the two straight pipes 14 are fixedly connected with electric, with external power source electric connection and control through control switch, the top of straight tube 14 runs through base 1 in proper order, places seat 2 and holds the chamber 3 intercommunication with the cylinder.

During operation, polyacrylamide protein gel is placed in the two cylindrical cavities 3, then the two delivery pumps 102 are started, a coloring agent in a placing bottle 101 is sequentially conveyed into the cylindrical cavities 3 through the first folding pipe 103 and the second folding pipe 104 to perform dyeing operation, a vibration motor is started during dyeing to drive the placing seat 2 to vibrate, after the dyeing operation is finished, the electric control valve 15 is started, so that the coloring agent in the cylindrical cavities 3 sequentially flows into the recovery box 13 through the through holes 43 and the straight pipes 14, then distilled water polyacrylamide protein gel is added into the cylindrical cavities 3 to be rinsed, then a decoloring agent is added to be decolored, distilled water and the decoloring agent sequentially flow into the recovery box 13 through the through holes 43 and the straight pipes 14, after the decoloring operation is finished, the decoloring agent remains in the inner cavities of the cylindrical cavities 3, the pull rings 45 are pulled upwards during the process, and the collecting cover 41 is further driven to move upwards through the pull rods 44, and scrape the material effect to cylindrical chamber 3 for remaining coloring agent can collect at ring groove 42, spur pull ring 45 always, can take out polyacrylamide protein gel this moment, then until the both ends of U type pipe 9 and ring groove 42 inner wall contact, start small-size suction pump 6 this moment, will remain the decolorant and extract to collecting in the box 5 through U type pipe 9, connecting pipe 8, pipe 7.

It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. The utility model provides a full-automatic polyacrylamide protein gel dyeing decoloration appearance, includes base (1), its characterized in that: the top of the base (1) is fixedly connected with a placing seat (2), two sides of the top of the placing seat (2) are respectively provided with a cylindrical cavity (3), and the insides of the two cylindrical cavities (3) are respectively provided with a movable liquid removing mechanism (4);

the activity removes liquid mechanism (4) including collecting lid (41), the surface of collecting lid (41) and the inner wall sliding connection that the cylinder holds chamber (3), ring groove (42) have been seted up at the top of collecting lid (41), the center department at collection lid (41) top is run through and is seted up through-hole (43), the top fixedly connected with pull rod (44) of collecting lid (41), the top of pull rod (44) is run through and is placed seat (2) and extend to the outside of placing seat (2), the top fixedly connected with pull ring (45) of pull rod (44).

2. The full-automatic polyacrylamide protein gel dyeing and decoloring instrument according to claim 1, wherein the instrument comprises: the novel vacuum pump is characterized in that a collecting box (5) is fixedly connected to the top of the base (1) and located behind the placing seat (2), a small suction pump (6) is fixedly connected to the top of the collecting box (5), and a liquid outlet of the small suction pump (6) is communicated with the top of the collecting box (5) through a guide pipe (7).

3. The full-automatic polyacrylamide protein gel dyeing and decoloring instrument according to claim 2, wherein the instrument comprises: the liquid inlet of small-size suction pump (6) intercommunication has connecting pipe (8), the one end intercommunication that small-size suction pump (6) were kept away from in connecting pipe (8) has U type pipe (9), the both ends of U type pipe (9) run through two cylinders respectively and hold chamber (3) and extend to the inside that two cylinders held chamber (3).

4. The full-automatic polyacrylamide protein gel dyeing and decoloring instrument according to claim 1, wherein the instrument comprises: the both sides at base (1) top all are provided with conveying mechanism (10), two conveying mechanism (10) symmetry sets up, conveying mechanism (10) are including placing bottle (101), place bottle (101) and fix the top at base (1), the top fixedly connected with delivery pump (102) of base (1), the inlet intercommunication of delivery pump (102) has first bellows (103), the one end that delivery pump (102) were kept away from in first bellows (103) runs through and places bottle (101) and extend to the inside of placing bottle (101).

5. The full-automatic polyacrylamide protein gel dyeing and decoloring instrument according to claim 4, wherein the instrument comprises: a liquid outlet of the conveying pump (102) is communicated with a second folding pipe (104), and one end, far away from the conveying pump (102), of the second folding pipe (104) penetrates through the placing seat (2) and extends into the placing seat (2).

6. The full-automatic polyacrylamide protein gel dyeing and decoloring instrument according to claim 1, wherein the instrument comprises: the improved cylinder support is characterized in that four supporting rods (11) are fixedly connected to four corners of the bottom of the base (1), four supporting plates (12) are fixedly connected between the surfaces of the supporting rods (11), boxes (13) are recovered through fixedly connected to two sides of the tops of the supporting plates (12), the tops of the recovering boxes (13) are communicated with straight pipes (14) and two electric control valves (15), the surfaces of the straight pipes (14) are fixedly connected with one another, and the top ends of the straight pipes (14) sequentially penetrate through the base (1), place the base (2) and are communicated with the cylinder containing cavity (3).