RU2108106C1 - Pectinic preparation for treating diarrhea infections in humans and animals - Google Patents

Abstract

FIELD: medicine; treatment of gastrointestinal disorders. SUBSTANCE: inventive novelty of preparation is that it contains pectic. Administration of preparation reduces recovery time and excludes antibiotics and chemical drugs from treatment course. EFFECT: higher efficiency. 1 tbl

Description

 The invention relates to medical and veterinary microbiology and therapy, and in particular to means for influencing diarrheal conditions and pathogens of intestinal diarrheal infections, and can be used in medical and veterinary medical institutions.

 Known drugs (enteroseptol, etc.), which are used as etiotropic drugs [1].

 The disadvantage of using these drugs is the development of complications such as allergization of the body, the development of dysbiosis, which requires, in turn, additional treatment. In this case, drug-resistant variants of pathogens are quickly formed, which also complicates and lengthens the treatment. Economic damage will increase due to temporary loss of human disability or animal death.

 The closest in effectiveness to the proposed drug is the antibiotic chloramphenicol [2], used to treat intestinal infectious diarrhea. A mandatory standard course of treatment involves ingestion of 0.5 g four times a day for 5 days. In case of inefficiency, the course of treatment is extended to 7 days or the antibiotic is completely replaced and the course of treatment is repeated.

 A frequent complication of such intensive antibiotic therapy is the development of dysbiosis, which is accompanied by persistent diarrhea, exhaustion, and a decrease in the body's immune status.

 The objective of the invention is to obtain an antidiarrheal bactericidal drug that actively affects the causative agents of infectious diarrhea, the use of which is not accompanied by the development of complications characteristic of antibiotic and chemotherapy (dysbiosis, allergies, the development of resistant forms of pathogens, etc.).

 The problem is solved by using a pectin preparation, which is a food pectin (GOST 29186-91), highly purified, in a soluble form, which dramatically increases its bactericidal activity and healing properties. The drug is used in the form of 3-5 wt.% Aqueous solution.

The following uses of pectin are known:
1 - for the prevention of occupational diseases in industries associated with the use of toxic metals: people get fruit juices enriched with pectin, which binds metal ions in the human intestine and helps to remove them from the body [3];
2 - for the prevention of radiation injuries, for example, during the liquidation of the consequences of the Chernobyl accident; pectin interacts with radionuclides and this complex is naturally excreted from the body [4].

 When applying a 3-5% solution of pectin as a therapeutic agent for the treatment of infectious doors, pectin, being an organic acid (D-galacturonic acid), creates an increased acidity in the intestinal zone (pH 3.5-4.0), which is bactericidal factor for pathogens of intestinal infections in vitro and in vivo.

 The effect of the use of pectin is that the body achieves an effect on the causative agents of infectious diarrhea, which is comparable to antibiotic or chemotherapy, but in a shorter time period (on average, for 2-3 days) and without serious complications such as dysbiosis, etc.

 Antibiotics and chemotherapy are completely excluded from the course of treatment, which dramatically reduces the cost of treatment and gives a pronounced economic effect in conditions of massive intestinal infections. Cases of chronic intestinal infections with this method of treatment were not observed.

To prepare a pectin solution, the corresponding sample of the preparation is dissolved in water heated up to 70-80 ^o C or physiological saline using a mixer (mixer) until a homogeneous preparation is obtained for 20-25 minutes. Then the solution is heated in a water bath at 90-100 ^o C for 20 minutes, after which the preparation is ready for use. The solution should be stored in the refrigerator at a temperature of 6 ± 2 ^o C for 90 days. without losing its properties.

Example 1. 1 ml of a 5% solution of pectin is mixed with 1 billion microbial bodies of the causative agents of dysentery or other diarrheal infections. The mixture is incubated in a thermostat at 37 ^o C. The viability of pathogens is checked hourly by plating on the optimal nutrient medium (nutrient agar, broth, etc.). The viability of pathogens can be determined during the first hour of incubation. Then comes the irreversible death of microorganisms.

Example 2. Patient M. A., 20 years old. Was admitted to the clinic of infectious diseases N 1 of Omsk on 10/15/95 at 20.30 with complaints of weakness, fever, abdominal pain, and frequent loose stools. She fell ill on the same day in the morning: her body temperature increased to 39 ^o C, vomiting - one-time, after 2 hours the abdominal pain and loose stools joined 5-6 times. Directed by the clinic to the hospital.

Upon admission: general condition of moderate severity, body temperature 38 ^o C. The skin is pale. The pharynx is calm. In the lungs, vesicular breathing. Pulse 88 beats / min, rhythmic, medium filling. AD-110/70. Heart sounds are muffled. The tongue is dry, covered with a white coating. The abdomen is soft, painful in epigastrium during palpation. Liver, spleen - without features. Liquid stool with an admixture of mucus.

Analyzes on the first day of hospital stay: feces liquid, alkaline reaction; microscopy - completely mucus and white blood cells. Urine - ud. weight 1015; acidic reaction; protein 0.165 g%; glucose - p.; ex. flat the epithelium is completely; white blood cells (8-10) / 1; red blood cells unchanged (10-15) / 1; slime - completely. Complete blood count "HB-113 g / l, red blood cells - 3.4 • 10 ¹² / l, CP-0.9 reticulocytes - 5%, platelets 217.9 • 10 ⁹ / l, white blood cells 6, 9 • 10 ⁹ / l, stab 6%, segmented 72%, lymphocytes 17%, ESR 4 mm / h. Blood nitrates 31 μg / kg. Blood glucose 4.49 mmol / l.

 From the patient's feces, a culture of salmonella typhimurium was isolated. Treatment: pectin 3% solution of 25 ml 4 times a day - two days. Rehydron 1000 ml for 8 hours

The patient's condition began to deteriorate 8 hours after the start of treatment. From 16.10.96 the general condition is satisfactory, body temperature 36.4 ^o C, no complaints. The skin is clean. In the lungs, vesicular breathing. Pulse 68 beats. / min, rhythmic, blood pressure 120/80 mm Hg The tongue is moist, coated at the root, the abdomen is soft, painless on palpation. The chair 2 times a day is mushy.

 On the third day of the patient’s stay in the hospital, the general condition of the patient is satisfactory; a chair decorated without pathological impurities; analyzes of feces and urine are within normal limits. Bacteriological analysis of feces is negative. The patient was discharged on 10.19.95 g in satisfactory condition with a diagnosis of salmonellosis (copro culture of salmonella typhimurium) gastroenterological version, moderate course.

Example 3. 1 ml of the known antidiarrheal agent SMEKTA (aluminum magnesium silicate, France) was mixed with 1 billion microbial bodies of the causative agents of dysentery and salmonellosis. Mixtures of a 5% pectin solution with the same pathogens are similarly prepared. The mixtures are incubated in a thermostat at 37 ^o C. Viability is checked by hourly seeding on sensitive nutrient media. The results are presented in the table.

 The results shown in the table show the advantage of pectin, combining antidiarrheal and bactericidal action.

Literature
1. Mashkovsky M.D. Medicines M .: Medicine, 1984, v. 2, p. 307.

 2. Ibid., Vol. 2, p. 250.

 3. Trakhtenberg I. M. et al. On the prophylactic use of pectin in professional mercurialism // Occupational health and prof. diseases, 1980, N 7, p. 3-36.

 4. Guidelines for the use of pectins and pectin-containing products for therapeutic purposes. USSR Ministry of Health, USSR Academy of Medical Sciences // Kiev: Harvest, 1990, N 50, p. 49-89.

Claims (1)

A preparation for treating diarrhea of humans and animals, containing an active substance, characterized in that the active substance is pectin in the form of a solution obtained by dissolving it in water or physiological saline at 70 - 80 ^o With subsequent heating of the solution in a water bath at 90 - 100 ^o C.

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