RU2104024C1 - Species for prophylaxis and treatment of thrombosis - Google Patents

Abstract

FIELD: medicine, phytotherapy. SUBSTANCE: species has the following components, wt.-%: horse chestnut seeds, 7-12; licorice roots, 12-17; peppermint leaves or melissa herb, 20; haw-thorn fruits, 20; and rose fruits, the balance. Species shows effect both hemostasis system and cardiovascular and nervous and lipid and carbohydrate metabolism also. EFFECT: enhanced effectiveness of species. 8 tbl

Description

 The invention relates to medicine, namely to the creation of herbal products and can mainly be used for the prevention and treatment of thrombosis and thromboembolism of various etiologies.

 Medicinal plants with antithrombotic (anticoagulant) activity are known, such as chestnut, leuzea, patrinia, garlic, etc. (1-4).

 As antithrombotic and venotonic agents, preparations are used that contain the active ingredients of horse chestnut fruits - eskusan, eoflazide (5).

 Several multicomponent collections for the treatment of diseases of the cardiovascular system are known (6).

 The disadvantage of these fees is that they are not used to treat thrombosis and thrombotic complications. In addition, these fees are not official.

 There are several collections for this pathology, issued by the American company "ENRICH", one of which is closest to the problem being solved. The collection contains hawthorn, papaya fruit, dandelion root, fennel, bubbly fucus, shadran, licorice, echinacea, etc. (7).

 However, individual medicinal plants and extracts from them affect only certain etiological and pathogenetic links of thrombosis, while this pathology requires a complex effect, including both the effect on the hemostasis system itself, and on the cardiovascular system as a whole, the nervous system, lipid and carbohydrate metabolism.

 The objective of the invention was the development of antithrombotic collection based on available domestic raw materials, including a composition of medicinal herbs for the prevention and treatment of thrombosis and thromboembolism of various etiologies, as well as related or related diseases, such as atherosclerosis, coronary heart disease, neurosis, etc.

 This goal is achieved by the fact that for the treatment of these diseases, a collection of “casmin” is proposed, containing plants that can normalize lipid metabolism, beneficially affect the functioning of the nervous, cardiovascular and respiratory systems, gastrointestinal tract, kidneys; showing general strengthening, analgesic and anti-allergic properties.

Antithrombotic collection "casmin" is a mixture of finely ground medicinal plants of the following composition:
Seeds of horse chestnut ordinary - 7-12%
Licorice roots naked - 12-17%
Peppermint leaves or lemon balm herb - 20%
Hawthorn fruits - 20%
Rosehips - 35% (rest)
All plants included in the collection are official.

The listed plants were included in the collection based on the known spectrum of their pharmacological properties:
anticoagulant, venotonic and anti-inflammatory in chestnut;
anti-aggregation, anti-inflammatory, anti-ulcer, hypolipidemic and anti-allergic licorice;
sedative and analgesic in mint and lemon balm;
cardiotonic in hawthorn;
tonic (source of vitamins C and P), lipid-lowering and choleretic in rose hips.

 The specific action of the collection is provided primarily by the presence in its composition of triterpene glycosides, flavonoids and vitamins.

 The components of the collection are selected so that the specific antithrombotic action of chestnut, anti-aggregation and lipid-lowering activity of licorice are potentiated and supplemented. When choosing the ratio of the components of the collection also took into account the taste of the infusion of the collection and manufacturability of its manufacture.

 Experimental studies of the infusion of the proposed composition. The effect of collection on hemocoagulation was studied under in vitro experiments (0.1-5 mg / ml blood) and with single intravenous (100 mg / kg) and oral (500 mg / kg) injections in rabbits (weighing 2.5-4.0 kg). In vitro experiments have also investigated the effect of collection on ADP-induced rabbit platelet aggregation (Born, 1962).

 Assessment of the state of the hemostatic system was carried out according to generally accepted tests (Balud V.P. et al., 1980). The experimental results were processed by the variational method of variation statistics using Student's criterion (Belenky M.L., 1963).

In vitro experiments revealed a direct anticoagulant and anti-aggregation effect of the collection, which was expressed in the corresponding change (by 25-50%) in the parameters of hemocoagulation and aggregation (Table 1)
Animal experiments confirmed the presence of an anticoagulant effect in the collection. This effect was observed within 60 minutes after intravenous (Table 2) and within 120 minutes after intragastric (Table 3) injections of the infusion collection.

 The hypolipidemic effect of the infusion collection was studied on a model of hyperlipidemia in rats caused by a single intraperitoneal administration of WR-1399 triton at a dose of 250 mg / kg. The infusion was administered intragastrically at doses of 50 and 100 mg / kg. Control animals with hyperlipidemia received an adequate amount of water. Lipid levels were measured in rat serum 18 hours after the administration of newt.

 A decrease in the level of β-lipoproteins (by 13 and 58%) and triglycerides (by 21.5 and 20%) in the blood serum of rats with experimental hyperlipidemia was shown to be lower than the control, which indicates the presence of lipolipidemic collection properties (table. 4).

 When studying the sedative properties of the collection, it was shown that the introduction of the experimental infusion of the collection against the background of chloral hydrate reduced latent periods of sleep by 13.5-43.3%, depending on the dose compared to the control. At the same time, its duration increased by an average of 6.1-41.5% (Table 5). The experimental results indicate that the collection infusion potentiates the hypnotic effect of chloral hydrate, i.e. exhibits sedative properties.

 In the study of the analgesic activity of the collection using the model of "acetic acid cramps" in mice, it was shown that the collection infusion at a dose of 100 mg / kg shows a slight analgesic activity, which is manifested in a decrease in the number of cramps by 25.6% compared with the control (table. 6).

 When studying the gastroprotective properties of the infusion collection using the model of indomethational gastric ulcers in rats, it was shown that the introduction of the infusion collection reduced the incidence of ulcerative defects of the gastric mucosa of rats by 12%, the average area of ulcerative lesions, and 3.2 times the Pauls index compared with control (table. 7). The results obtained indicate the gastroprotective effect of the collection "casmin".

 When studying the diuretic activity of the collection, it was shown that under the influence of the infusion, urination in experimental animals is increased by 50% (p <0.01) compared with the control, combined with a slight decrease in the excretion of sodium ions in the urine (Table 8). These data indicate a pronounced diuretic effect of collecting "casmin".

When studying the acute toxicity of the collection, it was shown that, when administered intragastrically, the collection, in doses of 2.5-10 g / kg, did not cause the death of the animals during the 14 days of observation. The following parameters of acute toxicity of the infusion of the collection with its intraperitoneal administration were established: LD ₁₆ 870 mg / kg, LD ₅₀ 1800 (1538-2106) mg / kg and LD ₈₄ 3810 mg / kg, in terms of dry raw materials.

 The data obtained indicate that this collection in terms of acute toxicity for mice can be classified as practically non-toxic substances (5th toxicity category).

 Thus, experimental studies have confirmed the specific antithrombotic activity of the collection "casmin", supplemented by lipid-lowering, analgesic, sedative, gastroprotective and diuretic properties. Based on the established pharmacological activity of the collection in combination with a low level of toxicity and known data on the cardiotonic, anti-allergic, anti-inflammatory and choleretic properties included in the collection of components, the collection "casmin" can be recommended for widespread use as a therapeutic and prophylactic antithrombotic agent for thromboses of various etiology.

 The collection "casmin" is used in adults for the prevention and complex treatment of thrombosis of various etiologies, including thrombophlebitis, thromboembolic complications in myocardial infarction, embolic strokes, etc., to prevent thrombosis in the postoperative period, as well as for the prevention of atherosclerosis.

 Materials on the drug "casmin" were sent to the Pharmacological Committee of the Ministry of Health and Ministry of Health of the Russian Federation in order to obtain permission to use the collection in medical practice.

 The collection is prepared as follows: take the seeds of horse chestnut (10 g), licorice roots (15 g), peppermint leaves or lemon balm herb (20 g), hawthorn fruits (20 g) and rose hips (35 g).

 All collection components are crushed to particle sizes passing through a 4-3 mm sieve.

 In preparing the collection, the following are used: rose hips (Article 38 of the USSR State Pharmacopoeia of the X1 edition issue 2 on rose hips); fruits of hawthorn (article 32 of the State Pharmacopoeia of the USSR on the fruits of hawthorn); seeds of horse chestnut ordinary (Technical conditions TU - 64-4-75-96); licorice root bare (Article 573 of the State Pharmacopoeia of the USSR X edition on the licorice root); peppermint leaves (article 18 of the State Pharmac. USSR of the X edition on peppermint leaves); lemon balm herb (VFS project).

 The collection infusion is prepared as follows: 2 teaspoons of the collection are poured with boiling water (1 glass), covered with a lid and insisted for 30 minutes.

 As a medicinal product, the infusion of “Kasmin” is taken in a warm form, 1 glass 3 times a day after meals; as a prophylactic in 1/2 cup 2-3 times a day. The treatment course is 3-4 weeks.

 The proposed collection can be produced in filter bags and bags of laminated paper in a single dose, which expands the possibility and ease of use of the proposed tool.

Bibliography
1. Aleshinskaya E.E., Farmakol. and toxicol., 23 (4), 452-455 (1962).

 2. Kolkhir VK, et al., Abstracts (2nd Russian National Congress. "Man and Medicine".

 3. Kolkhir V.K. Abstract. Cand. Diss., M., 1983.

 4. Beretz A.J. - P. Gazenave, Planta Med., 57, (1) 68-72 (1991).

 5. Mashkovsky M.D. Medicines, M., 1993.

 6. Sokolov S.Ya., I.P. Zamotaev, Handbook of medicinal plants, M., 1984.

 7. Information collection about the products of the company "Enrich, Samara, 1996, S. 49.

Claims (1)

 A collection for the prevention and treatment of thrombosis, including the fruits of hawthorn and licorice roots, characterized in that it additionally contains horse chestnut seeds, peppermint leaves or lemon balm herb and rose hips in the following proportions, wt. Seeds of horse chestnut 7 12
Licorice roots 12 17
Peppermint leaves or lemon balm herb 20
Hawthorn fruits 20
Rose hips

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