RU210037U1 - TEMPERATURE MAINTENANCE DEVICE FOR SYNCHRONOUS CULTIVATION OF MICROORGANISMS - Google Patents

Abstract

The utility model relates to additional equipment for thermostating installations and can be used to maintain and control the temperature during the cultivation of microorganisms. The technical result is the possibility of setting and maintaining several temperature regimes during synchronous cultivation of microorganisms. To achieve it, a device for maintaining temperature conditions for synchronous cultivation of microorganisms is proposed, including Peltier modules configured to be connected to a power source, a control module containing a measuring unit connected to a sensor, a microprocessor connected to an indicator that fixes temperature values, while containing an aluminum radiator profile, on which ten Peltier TEC-12706 modules are soldered on top, made with the ability to connect to five power supplies 12 V 5 A, and three fans are soldered from below, made with the ability to connect to a power supply 12 V 6 A, while controlling Peltier modules are carried out through five field-effect transistors IRL3705Z fixed by soldering on top of an aluminum radiator profile using the Arduino Nano platform, the basis of which is a microcontroller based on the ATmega328, while ten waterproof digital temperature sensors are connected to the Arduino Nano platform by wires DS18B20 and five LCD 1602 displays via II2C/IIC adapter, soldered to the top side of the aluminum radiator profile. 1 ill.

Description

Technical field

The utility model relates to additional equipment for thermostating installations and can be used to maintain and control the temperature during the cultivation of microorganisms.

State of the art

Various designs of microorganism culture devices based on Peltier elements are known from the prior art and are commonly used in the microbiological industry.

A device for determining the microbial contamination of overalls is known (patent for invention RU 2495924). The device contains a Peltier module having cooled and heated surfaces, a power supply unit with stabilized and regulated outputs, a generator, a temperature control device, a recording device, an electrothermometer, and a thermostat. The thermostat contains a heating element, a test tube with culture fluid and electrodes in it, connected to a generator. The Peltier module is made with the possibility of placing a strip of filter paper on its cooled surface and then pressing the part of overalls contaminated with microbes to the filter paper strip, and placing a strip of filter paper with microbes from overalls in a test tube located in a thermostat. A thermostat, a temperature control device, a recording device, a generator are connected to the stabilized output of the power supply, and an electrothermometer and a Peltier module are connected to the regulated output of the power supply. The invention provides a simplification of the design of the device, the preservation of the viability of microorganisms, which will allow you to quickly assess their accumulation in the material of workwear.

The main disadvantage of the described design is that it is adapted to conducting experiments in the same temperature range within the framework of one experiment. The device is not an installation for cultivating microorganisms with a wide temperature range within a single experiment. Such a system is focused on solving predominantly biotechnological problems and does not allow experiments with a small amount of inoculum. Also, the device is bulky, difficult to sterilize and complex in design.

The closest in technical essence to the claimed utility model is a temperature control device (RU 72770 U1), containing a heat-insulating housing, inside which a temperature control object is placed, on the bottom of which there is a thermoelectric battery powered by a power source, one surface brought into thermal contact with an external radiator, characterized in that the other surface of the thermoelectric battery is brought into thermal contact with an internal radiator bathed in a heat-stabilizing substance and is made on Peltier elements, a sensor is additionally introduced into the thermostating device, located inside the thermally insulating housing, a control module containing a measuring unit connected to the sensor, a microprocessor , connected to an indicator that fixes the temperature values of the thermostated object, and to n-solid-state relays, which, through an LC filter, connect the power source to the Peltier elements included in the thermoelectric battery, and switching node that connects temperature-controlled objects.

The disadvantage of this device is the complexity of the design. Also, such a system is limited in the study of only one object of temperature control in the range of one temperature.

The technical problem to be solved by the claimed utility model is to maintain the high stability of the required parameters for cultivating microorganisms and to expand the functionality of the device.

Utility Model Disclosure

The technical result of the claimed utility model is the possibility of setting and maintaining several temperature regimes during synchronous cultivation of microorganisms.

To achieve the technical result, a device for maintaining temperature conditions for synchronous cultivation of microorganisms, including Peltier modules configured to be connected to a power source, a control module containing a measuring unit connected to a sensor, a microprocessor connected to an indicator that fixes temperature values, while containing an aluminum radiator a profile on which ten Peltier TEC-12706 modules are soldered on top, configured to be connected to five 12 V 5 A power supplies, and three fans are soldered to the bottom, configured to be connected to a 12 V 6 A power supply, while controlling Peltier modules are carried out through five IRL3705Z field-effect transistors fixed by soldering on top of an aluminum radiator profile by the Arduino Nano platform, the basis of which is a microcontroller based on the ATmega328, while ten waterproof digital dates are connected to the Arduino Nano platform by wires temperature sensors DS18B20, and five LCD 1602 displays via II2C/IIC adapter, soldered to the top side of the aluminum radiator profile.

Brief description of the drawings

The figure shows a diagram of the claimed device, where:

1 - Peltier elements TES-12706;

2 - Temperature sensor DS18 B20;

3 - Aluminum radiator profile;

4 - Arduino Nano platform;

5 - Field effect transistor IRL3705Z;

6 - Fans;

7 - Power supplies 12 V 5A;

8 - LCD display 1602;

9 - Power supply 12 V 6A.

Implementation of the utility model

Peltier elements TES-12706 1 in the amount of 10 pcs. designed to maintain certain temperature conditions. Temperature sensors DS18B20 2 in the amount of 10 pcs. designed to measure temperature in an environment with microorganisms. The Arduino Nano 4 platform is designed to process information received from temperature sensors DS18B20 2, as well as to control field-effect transistors IRL3705Z 5 in the amount of 5 pieces, which are designed to regulate the operation of Peltier TEC-12706 1 elements and transmit information to LCD displays 1602 number of 5 pieces, which are designed to display information about temperatures. Fans 6 in the amount of 3 pcs. designed to remove heat from the aluminum radiator profile 3. Power supplies 12 V 5A in the amount of 5 pcs. designed to power Peltier elements TES-12706 1. Power supply 12 V 6A is designed to power fans 6.

The device for maintaining temperature conditions for synchronous cultivation of microorganisms, the scheme of which is shown in the figure, consists of an aluminum radiator profile 3 to which Peltier elements TEC-12706 1 connected in pairs (5 pairs in total) are attached by soldering, one of the outputs of which is connected to power supplies 12 V 5A 7. The second output is connected to the IRL3705Z 5 field effect transistors through the drain. The sources of field-effect transistors IRL3705Z 5 are connected to ground (not shown in the figure). The drains and sources of field-effect transistors IRL3705Z 5 are interconnected using resistors (not shown in the figure). The gates of the IRL3705Z 5 field-effect transistors are connected through resistors (not shown in the figure) to the digital pin (D3-D7) of the Arduino Nano 4 platform.

The 1602 8 LCD display is soldered to the aluminum radiator profile 3 and connected via an II2C/IIC adapter (not shown in the figure) to the Arduino Nano 4 platform. SDA is connected to pin A4(SDA), SCL output is connected to pin A4(SCL). The VVC output of the DS18 B20 2 temperature sensors is connected to the 5V pin, the DATA output is connected to the D2 pin, the GND output is connected to ground, the GND and DATA outputs are connected to each other by a resistor (not shown in the figure).

The fans 6 are soldered to the underside of the aluminum radiator profile 3 and connected in parallel to the 12V 6A power supply 9.

Device for maintaining temperature regimes for synchronous cultivation of microorganisms operates as follows.

One pair of Peltier elements TES-12706 1 maintains one temperature, that is, a total of five temperature regimes are maintained in two repetitions. To change the heating mode to the cooling mode, and vice versa, change the polarity of connecting a pair of Peltier elements TEC-12706 1.

Flasks with the medium (not shown in the figure) in the amount of 10 pieces, in which the microorganisms under study are located, are installed on Peltier elements TEC-12706 1 connected in pairs.

Waterproof digital temperature sensors DS18B202 2 in the amount of 10 pcs. are placed in flasks in the medium with cultures to monitor and control the temperature in them. A barbotage is also installed for mixing crops with a humidifier, which looks like a sealed container with water through which air flows using a compressor (not shown in the figure).

Next, the sketch with the program in which the temperatures for the experiment are set is loaded onto the Arduino Nano 4 platform.

Arduino Nano 4 via USB port, 12V 5A power supply 7 and 12V 6A power supply 9 are connected to the network.

The temperatures measured by the DS18B202 2 temperature sensors and the temperatures set for the experiment are displayed on the 1602 8 LCD displays.

The sketch with the Arduino Nano 4 program is developed in the Arduino IDE development environment for Arduino projects. The displays use the LiquidCrystal_I2C.h library and the standard Wire.h library, which is used to communicate the microcontroller with devices and modules via the I2C interface. From the first library, the init() and backlight() functions are used, which are responsible for initializing displays and turning on the backlight, respectively. They also use the setCursor() and print() functions, which allow you to move the screen cursor to display characters to a specific place on the screens and print characters, respectively.

To operate the DS18B20 temperature sensors, the DallasTemperature.h and OneWire.h libraries are used. A OneWire object and a DallasTemperature object are created to work with sensors. The requestTemperatures() function requests the temperatures that the sensors detect. The getTempCByIndex() function allows you to write the temperature received from the sensors to a variable by its index.

They use the standard pinMode() function, which sets the mode of operation of the given input/output as an input or as an output, and the digitalWrite() function, which gives a HIGH or LOW value to a digital input/output.

After writing the sketch, the program is compiled and uploaded via USB port to the Arduino Nano 4 platform.

Temperatures are specified as float constants in the program sketch before the mandatory setup() and loop() functions and are displayed on the 1602 8 LCD displays. 127061 and is displayed on the LCD displays 1602 8. If, in the case of heating, the set temperature is lower than the temperature measured by the temperature sensors DS18 B20 2, then the Arduino Nano 4 platform suspends the operation of the Peltier TEC-127061 1 elements, that is, it suspends heating. In the case of cooling, if the temperature specified in the sketch is higher than the temperature measured by the DS18B20 2 temperature sensors, then the cooling stops and the Peltier elements TEC-127061 1 are turned off. Checking these conditions is repeated cyclically to maintain the set temperatures.

For example, ten flasks were filled with microorganism culture medium (BG11). Microbial culture Synechocystis sp. was added to the medium. Since the Peltier elements TEC-127061 1 are connected in pairs, that is, one temperature is reached on one pair, five temperatures were set in the sketch for each pair of Peltier elements TEC-127061 1:16°C, 20°С, 27°С, 33°С, 40°С, and the conditions for turning on/off the Peltier elements TES-12706 were corrected 1 work as long as the temperature set for the experiment is less than the temperature determined by the temperature sensors DS18B20 2; when the medium with microorganisms is heated, the Peltier elements TEC-12706 1 work until the temperature set for the experiment is higher than the temperature determined by the temperature sensors DS18B20 2. At room temperature 26°C for temperatures of 16°C, 20°C, the Peltier elements TEC -12706 1 were set in cooling mode, for temperatures of 27°С, 33°С, 40°С - in heating mode. The sketch was uploaded to the Arduino Nano 4 platform, 12 V 5A 7 and 12 V 6A 9 power supplies were connected to the network. Flasks with a medium with microorganisms were installed on Peltier elements TEC-12706 1. Temperature sensors DS18B20 2 were placed in a medium with microorganisms, and bubbling was set for each flask. Information about the temperatures in the medium and the set temperatures was displayed on LCD displays 1602 8. Within a few hours, the temperatures in the flasks with the medium were set to the temperatures set for the experiment. Further, these temperatures were maintained until the device was turned off. At the end of the experiment, we obtain flasks with a medium with microorganisms, cultivated at different five temperatures in two repetitions.

Also, the device allows you to maintain temperature conditions for the synchronous cultivation of two types of microorganisms. For example, 5 flasks were filled with microorganism culture medium (BG11). Microbial culture Synechocystis sp. was added to the medium. Another 5 flasks were filled with microbial culture medium (BG11-N). The microbial culture Anabaena sp. was added to the medium. Since the Peltier elements TEC-127061 1 are connected in pairs, that is, one temperature is reached on one pair, five temperatures were set in the sketch for each pair of Peltier elements TEC-127061 1:18°C, 22°C, 30°C, 37°C, 45°C, and the conditions for turning on/off the Peltier elements TES-12706 were corrected. 1 work as long as the temperature set for the experiment is less than the temperature determined by the temperature sensors DS18B20 2; when the medium with microorganisms is heated, Peltier elements TEC-12706 1 work until the temperature set for the experiment is higher than the temperature determined by temperature sensors DS18B20 2. At room temperature 26°C for temperatures of 18°C, 22°C Peltier elements TEC -12706 1 were set in cooling mode, for temperatures of 30°С, 37°С, 45°С - in heating mode. The sketch was uploaded to the Arduino Nano 4 platform, the power supplies 12 V 5A 7 and 12 V 6A 9 were connected to the network. For each pair of connected Peltier elements TEC-12706 1, a flask with a medium with Synechocystis sp microorganisms and a flask with a medium with Anabaena sp microorganisms were installed. Information about the temperatures in the medium and the set temperatures was displayed on LCD displays 1602 8. Within a few hours, the temperatures in the flasks with the medium were set to the temperatures set for the experiment. Further, these temperatures were maintained until the device was turned off. At the end of the experiment, we obtain flasks with a medium with Synechocystis sp microorganisms and flasks with a medium with Anabaena sp. microorganisms, while cultivation took place for two types of microorganisms under the same temperature conditions in the range of five temperatures.

The advantages of the proposed device are:

- able to set and maintain up to 5 specified temperatures within one experiment;

- has a small size;

- has a simple design.

Depending on the experimental goals, cultivation on the device can be carried out under various conditions, for example, parameters such as the composition of the medium for cultivating microorganisms, the ratio of the liquid and gas phases, its composition, and the level of illumination can be changed.

Thus, the claimed device can be used to conduct experiments on the cultivation of microorganisms at different temperatures within a single experiment, while the temperatures lie in a wide range. The setup is simple in design and programming, as well as small in size.

Claims (1)

A device for maintaining temperature conditions for synchronous cultivation of microorganisms, including Peltier modules configured to be connected to a power source, a control module containing a measuring unit connected to a sensor, a microprocessor connected to an indicator that fixes temperature values, characterized in that it contains an aluminum radiator profile , on which ten Peltier TEC-12706 modules are soldered on top, made with the ability to connect to five power supplies 12 V 5 A, and three fans are soldered on the bottom, made with the ability to connect to a power supply 12 V 6 A, while controlling the Peltier modules through five IRL3705Z field-effect transistors fixed by soldering on top of an aluminum radiator profile using the Arduino Nano platform, which is based on an ATmega328-based microcontroller, while ten DS18B20 waterproof digital temperature sensors are connected to the Arduino Nano platform with wires and five LCD 1602 displays via II2C/IIC adapter, soldered to the top side of the aluminum radiator profile.

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