RU2528779C1 - Unit for increasing suspensions of microorganisms - Google Patents

Abstract

FIELD: biotechnology.

SUBSTANCE: unit comprises successively mounted on the transport platform 1 and interconnected by pipelines 2 with locking and regulating device, at least one container for the nutrient medium 3, at least one container for increasing the suspensions 4, and the at least one container for increasing the stock culture of microorganisms 5. The container for increasing the stock culture of microorganisms 5 and the container for increasing the suspensions 4 have thermostatic jackets 6, 7 made on infrared heating elements, are provided with a system of sensors: sensor of temperature 8, 9, of acidity regulation 10, 11 and level 12, 13, the additional containers to discharge gases 14, 15, and the liquid 16, 17 controlling the acidity with the metering pumps 18, 19. The container for increasing suspensions and microorganisms has a pipeline for transferring the finished product by a pump and is provided with the compressor connected to it through the valve for supplying air by the compressor for pumping liquids using displacement with a gas "cap". The unit is provided with an electronic control unit 24 and a power supply unit 25. To increase the productivity of the unit, several containers for increasing microorganisms 4 can be mounted on the platform, which are connected in parallel or in series. On the platform one container for increasing the suspensions can be mounted, in which the nutrient medium is poured and the microorganisms are placed to obtain a large volume of microbial suspension with low concentration of microbial cells. The system of sensors, pumps and valves is made with the ability to connect to the power supply unit 25 through the controller 33 to the PC 34 which inputs are connected to the unit of audio and light indication 35, the display 36, the remote monitoring unit 37 and the GPS antenna 38.

EFFECT: improving serviceability and expanding the technological capabilities due to the use of the unit for increasing the suspensions of microorganisms in the field, reduction of energy consumption during the technological cycle.

4 cl, 5 dwg

Description

The invention relates to the field of microbiology and biotechnology, and in particular to mobile complexes for increasing the suspension of microorganisms in the field, and can be used in methods of biological land reclamation, cleaning of water surfaces and / or biological methods of increasing oil recovery.

There are a very large number of devices for the growth of microorganism cells, each of which is developed depending on the field of application, the microorganisms used, the composition of the nutrient medium, the intended use of the products obtained, the type of product obtained, the design features of containers, sensors, mixing devices, process control and monitoring systems process.

As an analogue of this invention, patent RU No. 2144952, IPC: С12М 1/00, publ. 01/27/2000 "Apparatus for growing microorganisms", includes two horizontal sealed containers of the same volume, communicated by overflow pipes. They are equipped with nozzles for supplying a nutrient medium and inoculum and for discharging the culture fluid with means for supplying sterile air and a device for alternately venting air from them. It contains exhaust pipes mounted on the upper parts of the tanks, solenoid valves and timers installed on them. The tanks are divided along the length of the vertical partitions into separate zones. Means for supplying sterile air to them consist of spargers located in the zones. Overflow pipes are mounted in pairs in pairs, and their outlet sections are located above the bubblers. The tank from which the culture fluid is discharged is equipped with a circulation pipe for the continuous flow of the latter from its middle zone to the feed zone of the other tank and a vertically installed overflow partition with a height of 0.6-0.7 tank heights to separate the culture fluid drainage zone from the growing zones . A disadvantage of the known installation are:

- operation only in stationary conditions, which prevents the buildup of a suspension of microorganisms in the field;

- significant consumption of electric grid energy;

- the need to connect to the main water supply, complexity, the need for qualified personnel to service the installation.

The closest in technical essence to the claimed technical solution (prototype) considered patent RU No. 2408720, IPC: S12M 1/02; S12M 1/04, publ. 01/10/2011 "Installation for the cultivation of microorganisms", including a column with a removable cover, a pipe for venting air, a nozzle for draining the culture fluid and wash water, pipes for supplying a nutrient medium, a titrating agent and a seed culture, a multi-level disk mixer with a drive placed in the column, an aerator . The column consists of two cylindrical containers placed one above the other. The upper tank is made of a larger diameter for placement in it in the transport position of the installation of the lower tank for growing the uterine culture and has a flange fastened at the bottom with fastening bolts for sealing the tanks. The shaft of the disk mixer is made compound. The lower part of the shaft is made tubular and placed in a container for growing uterine culture. The upper part of the shaft is made in the form of a continuous cylinder, mounted in a removable cover of the installation and inserted into the lower tubular part of the composite shaft of the mixer with the possibility of movement relative to each other. A disk is mounted on the upper cylindrical part of the shaft of the disk mixer with the possibility of moving along the shaft and fixing it by means of pins at a given height. On the lower tubular part of the shaft of the disk mixer, the disks are placed at the ends of the tubular lower part. The installation is equipped with hoisting mechanisms located under the flange of the upper tank for combining the tanks with each other in the working position of the plant.

The disadvantages of this invention are:

- the inability to start the technological cycle on the road, because the installation in working condition has large vertical dimensions and requires disassembly during transportation, which reduces the time of use of the installation when moving from one object to another;

- cannot be used to build up a suspension of microorganisms in the field, because requires steam sterilization at an overpressure of 0.1 MPa for 2 hours before the technological cycle and steaming after;

- due to the design of the agitator, there is a possibility of contamination on the rotating double-sided mechanical seal;

- significant consumption of electric grid energy;

- the need for additional equipment during maintenance;

- the need to connect to the main water supply.

The objective of the invention is to develop the design of the installation for the growth of microorganisms, suitable for operation in various field conditions, free from the disadvantages of the prototype.

The problem is solved using the characteristics indicated in the 1st claim, such as a plant for growing suspensions of microorganisms, which contains at least one container for a nutrient medium sequentially installed on a transport platform and interconnected by pipelines with shut-off and control equipment, at least one capacity for growing suspensions and at least one capacity for building a uterine culture of microorganisms, and a capacity for building a uterine culture tours of microorganisms and a container for growing suspensions are made with airlift mixing system and have thermostatic shirts made on infrared heating elements, equipped with a system of sensors: temperature, control acidity and level, as well as additional containers for venting gases and regulating acidity of the liquid with metering pumps, at this tank for building suspensions and microorganisms has a pipeline for pumping the finished product through a pump and is equipped with connected m thereto through the air inlet valve to pump the compressor liquid from the vessel via gas "caps", while the installation is provided with an electronic control unit and power supply unit.

According to claim 2 of the invention, the installation is equipped with several containers mounted on the platform for growing microorganisms, connected in parallel or in series to increase the productivity of the installation upon receipt of a suspension with a high concentration of microbial cells.

According to claim 3 of the claims, one platform is installed on the platform for growing suspensions with the possibility of filling the medium with it and introducing microorganisms to obtain a suspension of large volume microorganisms with a low concentration of microbial cells.

According to claim 4, the system of sensors, pumps and valves are connected to the power supply, through the controller to a personal computer, the inputs of which are connected to the sound and light indication unit, display, remote monitoring unit and GPS antenna.

The above set of essential features allows you to get the following technical result: improving ease of use, expanding technological capabilities, increasing productivity by increasing suspensions of microorganisms in the field in the process of land reclamation, cleaning water surfaces. Installation is mobile, it allows to increase the suspension of microorganisms at the place where it is necessary to use the suspension and on the way to the place of use; in this regard, it must meet a number of necessary requirements.

Firstly, it should provide optimal conditions for the growth of microorganisms with minimal energy consumption.

Secondly, the installation during the technological cycle should control: liquid level, temperature, acidity of the medium. In the variant with an optical sensor, additionally determine the density of the suspension, the value of which is used to standardize the finished product.

Thirdly, the installation should be equipped with devices with low power consumption.

Fourth, the installation should be easy to maintain during the upgrade process and after-sales service.

Fifthly, the installation should not have a need to connect to the main water supply.

These requirements are satisfied by the invention, which is illustrated by the following graphic materials:

- block diagram of the installation - figure 1;

- structural diagram of the electronic control unit - figure 2

- block diagram of the installation, options - figure 3, 4, 5.

Figure 1 shows a block diagram of one of the embodiments of the proposed technical solution, where:

1 - transport platform;

2 - pipelines;

3 - capacity for a nutrient medium;

4 - capacity for increasing the suspension;

5 - capacity for building uterine culture of microorganisms;

6 - thermostatic shirt capacity for building a uterine culture of infrared thermocouples;

7 - thermostatic "shirt" capacity for increasing the suspension;

8, 9 - temperature sensors;

10, 11 - acidity sensors;

12, 13 - level sensors;

14, 15 - containers for venting gases;

16, 17 - containers for regulating the acidity of the liquid;

18, 19 - metering pumps;

20 - compressor;

21, 32 - air supply valves;

22 - pipeline pumping the finished product;

23 - pump for pumping the finished suspension of microorganisms;

24 - electronic control unit;

25 - power supply;

26, 27 - valves of the exhaust pipe;

28 - valve pipe air supply;

29 - valve piping pumping uterine culture;

30, 31 - valves for pumping a nutrient medium;

39 - container lid;

40 - container cover;

41 - container cover.

The transport platform 1 of the mobile complex is, for example, a car trailer or a semi-trailer.

Figure 2 additionally shows:

33 - controller;

34 - PC;

35 - block sound and light indication;

36 - display;

37 - block remote monitoring;

38 - GPS antenna.

In figure 3, 4, 5, the numbering is identical to figure 1.

Installation for growing suspensions of microorganisms contains sequentially installed on a transport platform 1 and interconnected by pipelines 2 with shut-off and control equipment at least one container for a nutrient medium 3, at least one container for building suspensions 4 and at least one container for building uterine culture of microorganisms 5. The capacity for building up the uterine culture of microorganisms 5 and the capacity for building suspensions 4 have thermostatic shirts 6, 7, made on infrared heating elements are equipped with a system of sensors: temperature 8, 9, regulation of acidity 10, 11 and level 12, 13, as well as additional containers for venting gases 14, 15 and regulating the acidity of the liquid 16, 17 with metering pumps 18, 19, while for pumping liquids, a method of displacing a gas “cap” formed as a result of pumping air by a compressor 20 connected through an air supply valve 21 to a container for growing suspensions 4 having a pipe 22 for pumping out the finished product using pump 23 is used that one.

In the installation, infrared thermocouples, for example, “Heat Plus” manufactured by “Sagi Century”, are used as a thermostatic element of the system for maintaining the set temperature of the suspension of microorganisms. It is known that infrared radiation is selectively absorbed by water molecules and is well reflected from metallized surfaces.

In accordance with the invention, an additional solution to simplify installation is a mixing and sparging system. The device uses airlift mixing and at the same time aeration of a suspension of microorganisms. In a simplified version, for pumping from a tank for growing a uterine culture into a tank for growing a suspension and from a tank for a nutrient medium in a tank for building a uterine culture and in a tank for growing a suspension of microorganisms, a method of displacing a gas “cap”, which occurs as a result of blocking the air exhaust valve, is used from a container and air supply by a compressor to a container with a suspension of microorganisms and a container for preparing and / or storing a nutrient medium. This solution allows you to get rid of the design of expensive energy-efficient pumps for pumping liquids.

A concentrated suspension of microorganisms is pumped from the tank for building up the uterine culture to the tank for building, which is 2-10 times larger in volume. The pumped uterine culture is diluted with a new nutrient medium and again grows in the capacity for building. Then the finished suspension of microorganisms is used for its intended purpose.

In accordance with this invention, containers for growing uterine culture and containers for growing suspension of microorganisms can be equipped with liquid robots (not shown in the drawing), which are connected to devices for determining the density and count of suspension cells, including pipes for lid and / or bottom supply aerating gas and for the removal of a gaseous medium, respectively.

The installation is equipped with an electronic control unit 24 and a power supply unit 25.

The system of sensors, pumps and valves are connected to the power supply 25, through the controller 33 to a personal computer 34, the inputs of which are connected to the sound and light indication unit 35, the display 36, the remote monitoring unit 37 and the GPS antenna 38 (Figure 2).

In accordance with the invention, for autonomy, a power supply, for example, storage batteries and / or internal combustion generators and / or autonomous solar power systems, can be installed in the installation.

In an additional embodiment, the installation may consist of one tank for building and one tank for the nutrient medium (figure 3). In this case, building up a suspension of microorganisms in a small volume with subsequent dilution of the nutrient medium requires separate installation of heating elements to reduce energy consumption during temperature control.

Another option involves the use of only one capacity for building (figure 4), in which at the beginning of building prepared liquid nutrient medium or filled ready. In this embodiment, the invention allows to increase the suspension without the use of additional containers. The use of this option is preferable for building immediately large volumes of a suspension of microorganisms used in high-cost methods. For example, to build up the Devoroil suspension and then use it for land reclamation on the transport platform, several tanks can be located connected in series (Figs. 1, 3, 4) or in parallel (Fig. 5) according to the options indicated above and having the ability to move with using road, rail, river and sea transport to any region of the country but according to the requirements of customers.

The following are the installation options.

Option 1.

In the initial state, all installation valves are closed. If it is necessary to start the technological cycle, the cover 41 of the container for the nutrient medium 3 opens (Fig. 1), through which the nutrient medium is introduced and the cover 39 of the container for building up the uterine culture 5 opens, through which the biological product or microorganism strains are introduced. The power supply unit 25 and the electronic control unit 24 are turned on. The parameters for increasing the suspension of microorganisms are set and the cycle starts. Valves 30, 31 and 32 are opened, the compressor 20 is turned on, and from the container for the nutrient medium 3, the nutrient medium is pumped into the container for building up the uterine culture 5 and the container for building the suspension 4 by pumping air into the container and displacing the nutrient medium. The thermostatic "shirt" is turned on 6. After filling the level with the nutrient medium, the level sensor 12 is activated, which in turn sends a signal to the controller 33 (Fig. 2), after which the control signal closes valve 31, then the tank 4 is filled, and then the valves 30 and 32, the valves 26 and 28 are closed and the aeration and mixing process starts in the tank 5. If the pH value deviates from the set acidity sensor 10 sends a signal to the controller 33, which in turn supplies a control signal to the pump metering 18 and adjusts the acidity of the medium to a predetermined value by correcting pumping liquid from the container 16 into the container 5. After building stock culture in the vessel 5 is closed and valve 26 open and valves 27, 29, a stock culture is reloaded into the container 4 through the displacement gas "cap". The thermostatic “shirt” 6 is turned off and the thermostatic “shirt” 7 is turned on. After overloading the uterine culture, valves 28, 29 are closed and valve 21 is opened, the aeration and mixing process in the tank 4 starts. If the pH value deviates from the set value, the acidity sensor 11 gives a signal to the controller 33, which in turn supplies a control signal to the metering pump 19 and corrects the acidity of the medium to a predetermined value by pumping the correction fluid from the tank 17 to the tank 4. After the completion of suspension of microorganisms in the tank 4 closes the valve 27, the thermostatic "shirt" 7 is turned off and the pump 23 is turned on for the subsequent targeted use of the suspension.

Option 2

In the initial state, all installation valves are closed. If it is necessary to start the technological cycle, the cover 41 of the container for the nutrient medium 3 opens (Fig. 1), through which the nutrient medium is introduced and the cover 39 of the container for building up the uterine culture 5 opens, through which the biological product or microorganism strains are introduced. The power supply unit 25 and the electronic control unit 24 are turned on. The parameters for increasing the suspension of microorganisms are set and the cycle starts. Valves 30, 31 and 32 are opened, the compressor 20 is turned on, and from the container for the nutrient medium 3, the nutrient medium is pumped into the container for building up the uterine culture 5 and the container for building the suspension 4 by pumping air into the container and displacing the nutrient medium. The thermostatic “shirt” is turned on 6. After filling the level with the nutrient medium, the level sensor 12 is triggered, which in turn sends a signal to the controller 33, after which the control signal closes valve 31, then the tank 4 is filled, after which the valves 30 and 32 are closed and valves 26 and 28 are opened, the aeration and mixing process in the tank 5 starts. If the pH value deviates from the set value, the acidity sensor 10 sends a signal to the controller 33, which in turn gives a control signal to the dose pump uyuschy 18 and adjusts the acidity of the medium to a predetermined value by correcting pumping liquid from the container 16 into the container 5. After building stock culture in the vessel 5 the valve 26 closes and opens the valves 27 and 29, a stock culture is reloaded into the container 4 through the displacement gas "cap". Part of the uterine culture remains in tank 5 for the new cycle. The cycle repeats in the above sequence. The thermostatic “shirt” is turned on 7. After overloading the uterine culture, the valves 28, 29 are closed and the valve 21 is opened, the aeration and mixing process starts in the tank 4. If the pH value deviates from the set value, the acid sensor 11 sends a signal to the controller 33, which in turn sends a control signal to the metering pump 19 and adjusts the acidity of the medium to a predetermined value by pumping a correction fluid from tank 17 to tank 4. After closing the suspension of microorganisms in tank 4, close it etsya valve 27 and the pump 23 is activated for the subsequent target of use suspension, and the cycle is repeated in sequence as described above. Thus, the mobile complex allows you to increase the suspension of microorganisms only once, introducing a biological product or strains of microorganisms, allowing you to reduce the cost of the increased suspension.

The invention allows to expand the potential for land reclamation, to increase oil recovery due to:

1. The use of containers with infrared heating elements forming a thermostatic “shirt”, information collection elements and modules installed on a transport platform, which allows for the growth of microorganisms in the path and at the pollution site and, as a result, to increase oil recovery.

2. The presence of a tank for building up the uterine culture makes it possible to obtain a suspension with a large number of microbial bodies.

3. The use of airlift system of mixing and aeration eliminates the installation of complex mixing devices.

4. The introduction of a sound and light indication unit, a remote monitoring unit allows you to select any extension mode and provide a more efficient and reliable installation control mode.

Thus, the use of this installation can significantly improve the technical and economic indicators for increasing the suspension of microorganisms.

The economic effect of the introduction of this installation can be obtained due to the following factors:

1. Significant savings in the consumption of purchased biological product and / or strain cultures, since once a biological product and / or strain cultures are introduced into the plant, it is possible to carry out a number of technological cycles.

2. Saving the cost of increasing the suspension through the use of energy-saving and cheap devices.

3. Providing, thanks to the module for the collection of information, data transmission and the development of control actions and the remote monitoring unit (regardless of the human factor), the objectivity of the information received and the automation of maintaining the specified parameters for increasing the suspension, which ensures a reduction in losses from its unreliability, which, in this case, can reach significant amounts.

The joint use of the above factors contributes to the organization of a profitable, waste-free and environmentally friendly way of soil remediation and enhanced oil recovery.

Although the present invention is described by way of examples and drawings, the scope of the present invention is not limited to these examples, but is defined only by the claims, taking into account possible equivalents.

Claims (4)

1. Installation for growing suspensions of microorganisms, characterized in that it contains sequentially installed on a transport platform and interconnected by pipelines with shut-off and control equipment at least one tank for a nutrient medium, at least one tank for growing suspensions and at least one a capacity for building up the uterine culture of microorganisms, and a capacity for building up the uterine culture of microorganisms and a capacity for building suspensions are made with airlift the mixing system and have thermostatted shirts made on infrared heating elements, equipped with a system of sensors: temperature, control acidity and level, as well as additional containers for venting gases and regulating the acidity of the liquid with metering pumps, while the tank for building suspensions and microorganisms has a pipeline for pumping of the finished product by means of a pump and is equipped with a compressor connected to it through the air supply valve for pumping liquid from the tank and using a gas "cap", and the setting unit is provided with electronic control and power supply unit. 2. Installation according to claim 1, characterized in that it is equipped with several containers mounted on the platform for growing microorganisms, connected in parallel or in series to increase the productivity of the installation upon receipt of a suspension with a high concentration of microbial cells. 3. Installation according to claim 1, characterized in that one platform is installed on the platform for growing suspensions with the possibility of filling the medium with it and introducing microorganisms to obtain a suspension of large volume microorganisms with a low concentration of microbial cells. 4. Installation according to claim 1, characterized in that the system of sensors, pumps and valves are connected to the power supply, through the controller to the personal computer the inputs of which are connected to the sound and light indication unit, display, remote monitoring unit and GPS antenna.

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