CN205420440U - A portable constant temperature culture apparatus for zebra fish embryo and juvenile fish - Google Patents
A portable constant temperature culture apparatus for zebra fish embryo and juvenile fish Download PDFInfo
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- CN205420440U CN205420440U CN201620233007.2U CN201620233007U CN205420440U CN 205420440 U CN205420440 U CN 205420440U CN 201620233007 U CN201620233007 U CN 201620233007U CN 205420440 U CN205420440 U CN 205420440U
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Abstract
The utility model relates to a portable constant temperature culture apparatus for zebra fish embryo and juvenile fish, belong to biological assay equipment technical field, the device is including hollow casing, the temperature control subtotal is located the electrical heating part of casing, the casing outside is equipped with the cultivation card slot, electrical heating part is including aqueous medium liquid and be arranged in the electrothermal tube of aqueous medium liquid, the temperature control part is including being located the inside temperature control circuit of casing, temperature sensor and the temperature regulation button that is located housing face, the temperature display screen, be equipped with switch and USB interface on the casing. This device simple structure, safe in utilization, convenient, through the USB interface connection portable power source power supply, the energy acquires the convenience, can guarantee the experimentation in the zebra fish embryo invariable with the ambient temperature that the juvenile fish locate, the accuracy of improvement experimental result, the while can be at the required normal temperature within range of embryonic development, according to experiment needs temperature regulation to satisfy the temperature demand of different experiments.
Description
Technical field
This utility model relates to a kind of portable constant temperature culture apparatus for zebrafish embryo Yu juvenile fish, belongs to biotic experiment equipment technical field.
Background technology
Brachydanio rerio is a kind of low profile thermal Trichiurus haumela.Owing to egg laying amount is big, the feature such as easily collecting, raising be simple, become the recommendation test species of ecotoxicological standard.Additionally, Brachydanio rerio is also the ideal model of research developmental biology, molecular genetics, medicine evaluating drug effect in early days and toxicity screening etc., purposes is quite varied.
Brachydanio rerio in embryo and juvenile fish stage to the resistivity of variations in temperature well below the adult fish stage.Standard temperature for Zebrafish Embryo is 28.5 DEG C both at home and abroad.In the range of 25～33 DEG C embryo still can normal development, beyond this temperature range, hatch for a long time and will appear from exception.But in normal development temperature, even the physiological functions such as zebrafish embryo and the rate of development of juvenile fish, consumption rate, heart rate also can be impacted by temperature fluctuation by a small margin.
Owing to zebrafish embryo is ectogenesis and transparent, can directly viviperception, therefore zebrafish embryo and juvenile fish are commonly used for all kinds of Coloration experiment.As neutral red staining live body Brachydanio rerio studies Macrophage Inflamatory behavior, Nile red dyeing Brachydanio rerio research gastrointestinal motility situation, calcein dyeing Brachydanio rerio research mineralization of skeleton situation etc..In all kinds of Coloration experiments, under identical dyeing time, ambient temperature is bigger on Color impact.Ensure that in experimentation, dyeing temperature is consistent, can improve accuracy and the repeatability of experimental result.
Therefore in practical operation, experimenter should be according to experiment needs, and one side regulate and control the speed of fetal development by suitably adjusting temperature, on the other hand should ensure that and operates the constant of temperature and unification in identical experiment, improves the repeatability of experiment, the reliability of result.In existing experimental implementation, embryo juvenile fish is positioned in incubator cultivation, and incubator volume is bigger, it has not been convenient to carry；When needs are tested, generally the juvenile fish in incubator or embryo are taken out in the environment being placed in laboratory, the heating indoor devices such as air-conditioning are relied on to ensure that laboratory has certain temperature environment, but air-conditioning is the most insensitive the most uneven for the heating of room temperature, the embryo or the juvenile fish that are exposed in room temperature are highly prone to temperature fluctuations affect, bring puzzlement greatly to existing experimentation, therefore, need a kind of portable constant temperature culture apparatus for zebrafish embryo Yu juvenile fish of design badly.
Utility model content
For the deficiencies in the prior art, this utility model provides a kind of portable constant temperature culture apparatus for zebrafish embryo Yu juvenile fish, temperature scalable, can guarantee that the temperature constant cultivating the zebrafish embryo in board slot and juvenile fish local environment in experimentation simultaneously.
The technical solution of the utility model is as follows:
A kind of portable constant temperature culture apparatus for zebrafish embryo Yu juvenile fish, including the housing of hollow, temprature control unit and the heating partial being positioned at housing, hull outside is provided with cultivation board slot, heating partial includes aqueous medium liquid and the electrothermal tube being positioned in aqueous medium liquid, temprature control unit includes being positioned at the temperature-control circuit of enclosure interior, temperature sensor and being positioned at the temperature adjustment buttons of surface of shell, temperature display, and housing is provided with on and off switch and USB interface.Cultivating board slot to be used for placing culture plate or culture dish, USB interface can be directly connected to computer by power line, it is possible to accesses power supply by power line and attaching plug, is convenient for carrying.Aqueous medium liquid is high energy storage pyrogenicity liquid and the mixture of water, quick heating, and persistently, the heat of available buffer electrothermal tube generation, makes the temperature of cultivation board slot inner space reach to test required design temperature by heat transfer, it is to avoid local temperature is too high the most again in insulation.Temprature control unit can regulate temperature in the range of 15.00～40.00 DEG C.
Preferably, described temperature display includes setting temperature value display screen and measuring temperature value display screen.
Preferably, described temperature adjustment buttons includes that temperature setting button, temperature down button and temperature raise button.
Preferably, described electrothermal tube is to be located at the coil bottom enclosure interior.Coil is roundabout is arranged in housing bottom, makes heating the most evenly.
Preferably, described temperature sensor is located at outside cultivation board slot.Cultivate board slot to be located on housing, the inner side cultivating board slot is to place culture plate or the space of culture dish, cultivate the aqueous medium fluid space being the heating being positioned at housing outside board slot, make temperature sensor nearest from cultivating board slot, it is simple to measure the temperature conditions cultivated in board slot for holding zebrafish embryo or juvenile fish more accurately.
Preferably, described housing also includes groove lid, and groove lid is connected with housing outer surface by hinge.Groove lid, for the space cultivated in board slot being maintained temperature further in experimentation, reduces heat material and scatters and disappears.
It is further preferred that the size of described constant temperature culture device is length × width × height is 300mm × 200mm × 100mm.
It is further preferred that the size of described cultivation board slot is length × width × height is 150mm × 100mm × 40mm.
The beneficial effects of the utility model are:
1, the portable constant temperature culture apparatus for zebrafish embryo Yu juvenile fish described in the utility model, heating partial and temprature control unit can be passed through, in controlling to cultivate board slot, temperature keeps constant, ensure zebrafish embryo and juvenile fish local environment temperature constant in experimentation, improve the accuracy of experimental result.
2, the portable constant temperature culture apparatus for zebrafish embryo Yu juvenile fish described in the utility model, can need to regulate temperature according to experiment in the normal temperature range needed for fetal development, high temperature promotes Zebrafish Embryo, low temperature delays fetal development, to meet the demand of different experiments.
3, the portable constant temperature culture apparatus for zebrafish embryo Yu juvenile fish described in the utility model, simple in construction, use conveniently, safely, heater connects portable power source by USB interface and powers, and the energy obtains convenient.
Accompanying drawing explanation
Fig. 1 is the structural representation of this utility model portable constant temperature culture apparatus；
Fig. 2 is the section fluoroscopy schematic diagram of this utility model portable constant temperature culture apparatus；
Wherein: 1, cultivate board slot, 2, cultivate board slot inwall, 3, housing exterior walls, 4, on and off switch, 5, electrothermal tube, 6, temperature adjustment buttons, 61, temperature setting button, 62, temperature down button, 63, temperature raise button, 7, temperature value display screen is set, 8, temperature value display screen is measured, 9, USB interface, 10, power line, 11, attaching plug, 12, groove lid.
Detailed description of the invention
Below by embodiment and combine accompanying drawing this utility model is described further, but it is not limited to this.
Embodiment 1,
nullA kind of portable constant temperature culture apparatus for zebrafish embryo Yu juvenile fish，As shown in Fig. 12，Housing including hollow、Temprature control unit and the heating partial being positioned at housing，Hull outside is provided with cultivation board slot，Heating partial includes aqueous medium liquid and the electrothermal tube being positioned in aqueous medium liquid，Temprature control unit includes the temperature-control circuit being positioned at enclosure interior、Temperature sensor and be positioned at the temperature adjustment buttons of surface of shell、Temperature display，Temperature-control circuit sets for the temperature controlling whole device，Temperature sensor is for measuring the temperature regime cultivated near board slot，Temperature display includes setting temperature value display screen and measuring temperature value display screen，Temperature sensor is located at outside cultivation board slot，Cultivate board slot to be located on housing，The inner side cultivating board slot is to place culture plate or the space of culture dish，Cultivate the aqueous medium fluid space being the heating being positioned at housing outside board slot，Make temperature sensor nearest from cultivating board slot，It is easy to measure the temperature conditions cultivated in board slot for holding zebrafish embryo or juvenile fish more accurately.Electrothermal tube is the roundabout coil being located at bottom enclosure interior, as it is shown in figure 1, to add hot liquid, the heat that liquid available buffer electrothermal tube produces, make the temperature of cultivation board slot reach to test required design temperature by heat transfer.
Housing exterior walls is made up of adiabator, and housing is provided with on and off switch and USB interface.Cultivating board slot to be used for placing culture plate or culture dish, USB interface can be directly connected to computer by power line and obtain power supply, it is possible to accesses power supply by power line and attaching plug, is convenient for carrying.Aqueous medium liquid is high energy storage pyrogenicity liquid and the mixture of water of commercially available gained, quick heating, insulation is lasting, the heat that available buffer electrothermal tube produces the most again, the temperature of cultivation board slot inner space is made to reach to test required design temperature by heat transfer, it is to avoid local temperature is too high.Temprature control unit can regulate temperature in the range of 15.00～40.00 DEG C.Power-on switchs, and sets temperature value display screen and measurement temperature value display screen is bright, and whole device comes into operation.Raise button 63 by temperature setting button 61, temperature down button 62 and temperature and be adjusted to test temperature required, the temperature of overview setup temperature value display screen 7 display simultaneously is the design temperature that experiment is required, after the temperature of temperature value display screen 8 to be measured display reaches design temperature, experiment can be started.
The size of constant temperature culture device be length × width × height be 300mm × 200mm × 100mm, cultivate board slot size be length × width × height be 150mm × 100mm × 40mm, be suitable to routine experimentation operation.
Embodiment 2,
A kind of portable constant temperature culture apparatus for zebrafish embryo Yu juvenile fish, as described in Example 1, difference is its structure, and housing also includes groove lid, and groove lid is connected with housing outer surface, as shown in Figure 2 by hinge.Groove lid, for the space cultivated in board slot being maintained temperature further in experimentation, reduces heat material and scatters and disappears.
Claims (8)
1. the portable constant temperature culture apparatus for zebrafish embryo with juvenile fish, it is characterized in that, including the housing of hollow, temprature control unit and the heating partial being positioned at housing, hull outside is provided with cultivation board slot, heating partial includes aqueous medium liquid and the electrothermal tube being positioned in aqueous medium liquid, temprature control unit includes being positioned at the temperature-control circuit of enclosure interior, temperature sensor and being positioned at the temperature adjustment buttons of surface of shell, temperature display, and housing is provided with on and off switch and USB interface.
Portable constant temperature culture apparatus for zebrafish embryo Yu juvenile fish the most according to claim 1, it is characterised in that described temperature display includes setting temperature value display screen and measuring temperature value display screen.
Portable constant temperature culture apparatus for zebrafish embryo Yu juvenile fish the most according to claim 1, it is characterised in that described temperature adjustment buttons includes that temperature setting button, temperature down button and temperature raise button.
Portable constant temperature culture apparatus for zebrafish embryo Yu juvenile fish the most according to claim 1, it is characterised in that described electrothermal tube is to be located at the coil bottom enclosure interior.
Portable constant temperature culture apparatus for zebrafish embryo Yu juvenile fish the most according to claim 1, it is characterised in that described temperature sensor is located at outside cultivation board slot.
Portable constant temperature culture apparatus for zebrafish embryo Yu juvenile fish the most according to claim 1, it is characterised in that described housing also includes groove lid, groove lid is connected with housing outer surface by hinge.
Portable constant temperature culture apparatus for zebrafish embryo Yu juvenile fish the most according to claim 1, it is characterised in that the size of described constant temperature culture device be length × width × height be 300mm × 200mm × 100mm.
Portable constant temperature culture apparatus for zebrafish embryo Yu juvenile fish the most according to claim 1, it is characterised in that the size of described cultivation board slot be length × width × height be 150mm × 100mm × 40mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620233007.2U CN205420440U (en) | 2016-03-24 | 2016-03-24 | A portable constant temperature culture apparatus for zebra fish embryo and juvenile fish |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620233007.2U CN205420440U (en) | 2016-03-24 | 2016-03-24 | A portable constant temperature culture apparatus for zebra fish embryo and juvenile fish |
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| CN205420440U true CN205420440U (en) | 2016-08-03 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107460124A (en) * | 2017-09-18 | 2017-12-12 | 阜阳师范学院 | A kind of animal embryo culture apparatus |
| CN108982826A (en) * | 2018-09-28 | 2018-12-11 | 广东工业大学 | Test device and test method of a kind of zebra fish detection nano particle to embryotoxicity |
| CN111394247A (en) * | 2020-03-31 | 2020-07-10 | 哈尔滨工业大学 | Constant-temperature heating system for cell in-situ culture |
-
2016
- 2016-03-24 CN CN201620233007.2U patent/CN205420440U/en active Active
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107460124A (en) * | 2017-09-18 | 2017-12-12 | 阜阳师范学院 | A kind of animal embryo culture apparatus |
| CN108982826A (en) * | 2018-09-28 | 2018-12-11 | 广东工业大学 | Test device and test method of a kind of zebra fish detection nano particle to embryotoxicity |
| CN108982826B (en) * | 2018-09-28 | 2024-03-26 | 广东工业大学 | Test device and test method for detecting embryotoxicity of nano particles by zebra fish |
| CN111394247A (en) * | 2020-03-31 | 2020-07-10 | 哈尔滨工业大学 | Constant-temperature heating system for cell in-situ culture |
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