RU2067450C1 - Method of cow endometritis treatment - Google Patents

Abstract

FIELD: veterinary. SUBSTANCE: method involves intrauteric administration of preparation containing lactobacilli of the strains Lactobacillus plantarum "Kirov-1" and Lactobacillus buchnery "Kirov-4". Preparation is administrated 2-3 times at interval for 24-48 hr. EFFECT: enhanced effectiveness of treatment.

Description

 The invention relates to veterinary medicine and is intended for the treatment of endometritis in cows. There are no analogues in veterinary medicine close to biosan.

 In the treatment of inflammatory processes in the uterus of cows, antibiotics, sulfonamide and nitrofuran drugs are currently widely used. However, the use of such drugs leads to negative results of addiction of microbes to them, a decrease in the effectiveness of treatment, and a deterioration in the quality of milk.

 A known method of treating inflammatory diseases of the vagina in women by intravaginal administration to patients of gelatin cylinders with a culture of vaginal rods / Doderlein sticks /. These bacilli are normal inhabitants of the vagina in women and have antagonistic properties against conditionally pathogenic microbes that are foreign to the vagina. However, the identification of the used microbial culture / lactobacilli / was not carried out, and the proposed method for the treatment of vaginitis and colpitis in women did not find practical application.

 The prototype for biosan according to the mechanism of action is the bacterial preparations lactobacterin and bifidumbacterin, which are a lyophilized microbial mass of living antagonistically active lactobacilli and bifidumbacteria. These drugs have been used for a long time to treat children and adults suffering from chronic colitis and dysbacteriosis. A number of authors have found that lactobacterin and bifidumbacterin can be used in the treatment of inflammatory diseases of the female genital area. The proposed method of treatment has received official recognition in medicine and is recommended for use in case of inflammation of the vaginal mucosa in women / vaginitis, colpitis /. In the treatment of inflammatory processes in the uterus in women / endometritis / lactobacterin and bifidumbacterin are not used. The feasibility of their use in endometritis in cows has not been studied.

 The purpose of the invention is to increase the efficiency of treatment of endometritis in cows, reduce the time and reduce the cost of treatment.

 This goal is achieved by the fact that in the treatment of cows with endometritis of cows, a new bacterial preparation biosan is used, which includes the association of two strains of animal-friendly lactobacillus-lactobacillus plantarum / Kirov-1 / and lactobacillus buchneri / Kirov-4 /, grown for meat-peptone hepatic broth / MPPB / with the addition of glucose and sodium chloride / 4 /.

 Lactobacilli used for the preparation of biosan are isolated from the vagina of healthy women with a first degree of purity. They have high antagonistic activity against Escherichia coli, Pseudomonas aeruginosa, staphylococci, streptococci and Proteus. After the introduction of lactobacilli into the uterus of the cows, the biosan take root on the mucous membrane for 15 or more days and inhibit the development of microbes that cause the inflammatory process.

When studying the cultural and biochemical properties of the strain of lactobacillus plantarum / Kirov-1 / it was found that lactobacilli are motionless gram-positive rods in smears are located singly or in uneven clusters. The strain grows well on MPPB, wort broth, medium MPC. MPC forms a uniform turbidity on the broth with the appearance of an abundant loose sediment at the bottom of the tube. On agar, MPC forms medium / 1.0-2.0 mm / convex, smooth, opaque white or light beige R- and S-colonies. Catalase-negative. Spore and capsules do not form. Indole and hydrogen sulfide does not emit, hemolysis of red blood cells does not cause. Sterile skim milk coagulates after 48-72 hours with the formation of a weak clot without separation of serum. The acid content in milk is 1.2%. The maximum acidity in degrees Turner is 120 ± 20 ^o T. The strain decomposes lactose, glucose, maltose, sucrose, sorbitol, mannitol, hydrolyzes galactose to acid without formation of gas, and does not cause the death of white mice with subcutaneous or intraperitoneal the introduction of daily culture at a dose of 500 million microbial cells, ferments glycogen.

 Cultural and biochemical properties of the strain of lactobacillus buchneri / Kirov-4 /.

 Lactobacilli strain-motionless gram-positive bacilli are located in smears alone or in uneven clusters. The strain grows well on MPPB, wort broth with or without chalk and forms a white loose sediment. On the medium, the MPC causes uniform turbidity with the appearance of an abundant loose sediment at the bottom of the tube. On a dense medium, MPC forms small colonies with a diameter of 0.5-1.0 mm. In appearance, the colonies are unpigmented, translucent, granular, often R-shaped. Catalase-negative. Spore and capsules do not form. Indole and hydrogen sulfide does not emit, hemolysis of red blood cells does not cause. Sterile skim milk does not clot.

 Enzymatic properties. It breaks down to acid without gas formation arabinose, galactose, maltose and with the formation of gas glucose. Does not ferment mannitol, sorbitol, raffinose, sucrose, dulcite, mannose and lactose. Does not cause the death of white mice with subcutaneous or intraperitoneal administration of a daily culture at a dose of 500 million microbial cells.

 Biosan is a light brown liquid with a grayish precipitate at the bottom of the bottle. The sediment during agitation is easily broken and causes turbidity of the drug. Biosan is produced in veterinary laboratories and produced in packs of 12.50 or 100 ml in glass bottles closed with rubber stoppers and metal caps. In a single dose / 4 ml / contains 12-15 billion lactobacilli.

 The optimal dose of biosan was determined in an experiment in the treatment of chronic purulent-catarrhal endometritis in 17 cows of black-motley breed. At the same time, 4 ml of biosan with a content of 3.5 ml of lactobacilli in 1 ml were administered intracervically to 4 cows, to 4 cows of 4 ml of biosan with 3 billion lactobacilli in 1 ml, and to 5 and 4 cows containing less than 3 billion lactobacilli in 1 ml of the drug.

 The therapeutic efficacy of various doses of biosan turned out to be the same and amounted to 100%. However, the fertility of cows in the groups after treatment was different. In groups of cows treated with biosan containing 3-3.5 billion lactobacilli in 1 ml / 12-15 billion live microbes in a dose / after 2 inseminations, all cows were fertilized / 100% /, and in groups treated with doses containing less than 3 billion lactobacilli in 1 ml, the fertility of cows from two inseminations was 60-75%. In subsequent experiments, similar results were obtained. Therefore, the optimal dose of biosan is 4 ml, with a content of 1 ml of 3-3.5 billion lactobacilli / 12-15 billion in a dose /.

 The effectiveness of treatment of endometritis with biosan was studied in 10 cows with a purulent-catarrhal form of endometrial inflammation, and in 63 cows with chronic endometritis. Clinical recovery occurred after 1-3 treatment procedures in all animals. Of these, 62 goals were fertilized (84.9%).

 Subsequently, under the methodological guidance of the authors, experiments to study the effectiveness of treating endometritis in cows were carried out on the farms of the Gorky Region, Sverdlovsk Region, and the Stavropol Territory. At the same time, the high efficiency of treatment of endometritis in cows with biosan was confirmed. For example, on the farms of the Petrovsky district of the Stavropol Territory, the 6044th cows were treated with endometritis. Of these, recovery occurred in 5600 goals / 92.7% /, 4678 goals / 83.7% / were fertilized after treatment.

 In 1986, employees of the Kuybyshev Regional Veterinary Laboratory carried out an official production check of the effectiveness of the treatment of endometritis in cows with biosan on the instructions of the Main Veterinary Administration of 02.27.85 N 177-8. At the Samara State Farm, 802 cows were treated with biosan. Of these, 682 cows (85.03%) recovered after a double intrauterine administration of biosan. Fertilization of cows after treatment was 100%. The therapeutic efficacy of biosan was higher than with neofur treatment of cows, the dosage and frequency of application of biosan was less.

Claims (1)

A method of treating endometritis in cows by intrauterine administration of a preparation based on lactobacilli, characterized in that a preparation containing Lactobacillus plantarum Kirov-1 and Lactobacillus buchnery Kirov-4 strains grown on meat-peptone-hepatic broth is used as a preparation based on lactobacilli with 2% glucose, and the drug is administered in 2 3 doses with a single dose of 4 cm ³ 3.0 3.5 billion lactobacilli 2 3 times with an interval of 24 48 hours