RU2061961C1 - Method for diagnosing periodontium tissues diseases - Google Patents

Abstract

FIELD: medicine. SUBSTANCE: method involves determining R-protein contents in gingival fluid. EFFECT: improved functional results.

Description

 The invention relates to medicine, namely to therapeutic dentistry.

 A known method for the diagnosis of periodontal disease, which consists in determining the content of lysozyme in mixed saliva according to the method of K. A. Kagramanova, Z. V. Ermolieva.

The study is carried out before breakfast. Mixed unstimulated saliva is collected in a test tube (by spitting). Whole unstimulated saliva is bred by physical. a solution in a ratio of 1 to 10, multiplying the result by 10. Saliva of 0.1 ml is applied to standard wells (10 mm in diameter) cut by a punch in the thickness of the agar. Samples are placed in a thermostat and incubated at 37 ^° C for 24 hours, after which the Micrococcus lysodeikticus lysis zones are measured around the well and the lysozyme concentration is determined by graded curves (constructed from a series of twofold dilutions in a physical solution of crystalline egg white lysozyme: from 1.56 to 250 μg / ml). The diameter of the lysis zones of Micrococcus lysodeikticus is directly dependent on the concentration of lysozyme in the test fluids (Kagramanova K. A. Ermolyeva Z. V. Comparative characteristics of methods for determining the activity of lysozyme. Antibiotics, 1966, N 10, p. 917 919).

 However, this method has drawbacks, because the concentration of saliva lysozyme during the day is subject to strong fluctuations that do not coincide in time in different people and even in one person on different days.

 The amount of lysozyme in saliva changes rapidly under the influence of ordinary physiological stimuli, neuropsychic influences, physical activity. In addition, the amount of lysozyme depends on the time of the year, on gender, in women on the phase of the menstrual cycle (D. Pletsy. Biological role of lysozyme and its therapeutic use. Karaganda, 1972, p.163 167; Fedortsov K. K. Kuzmin S. N. Kozlova N.N. Pershin B.B. Laboratory work. 1981. N 12, p. 735 736).

 The concentration of lysozyme in a single sample of saliva is not an objective criterion of magnitude. To do this, it is recommended to take 3 samples of saliva within 10 minutes each with a 30-minute interval from the start of collection (Yu. S. Varenko, V.T. Shevchenko, L.P. Zhovnirenko. Laboratory. 1987, N 5, p. 366 370).

 There is a method of determining the level of R-protein in peripheral venous blood, used in the clinic of infectious diseases (Regulatory R-proteins for infectious and other diseases. Sat. N. Proceedings of the NIIEM named after N. F. Gamalei of the Academy of Medical Sciences of the Russian Federation. Moscow. 1990).

 The purpose of the invention is the simplification of the method for the diagnosis of periodontal tissue diseases, improving accuracy with minimal time.

 This goal is achieved by determining the R-protein in the gingival blood.

 Studies were carried out in 2 groups of patients: 1 group with periodontal diseases without background pathology, 2 group with background pathology. The sampling was carried out as follows: 0.05 ml of gingival blood (obtained as a result of curettage of pathological tooth-gingival pockets) was taken with an elongated Pasteur pipette into a test tube containing 0.05 ml of 0.9 solution of NaCl with the blood preservative Glyugitsir ( 1 volume of the solution "Glyugitsir" + 3 volumes of 0.9 solution of Na-solution), 0.05 ml of blood from the patient’s finger was taken with a scarf pen and capillary. They were introduced into another test tube with the Glugitsira solution 1 4, both tubes were centrifuged for 10 min at 2000 rpm, the supernatant was separated and used to determine R-proteins. The erythrocyte sediment was discarded.

 Next, the studied sera were added to the test system (anti R-serum + red blood cells 0/1 / Rh / + /) and the titer of the R-protein was determined.

 The R-protein titer is considered the last dilution of blood serum, which gives an inhibition of the test system (the inhibition of hemagglutination inhibition between anti-R-serum and human red blood cells 0/1 / Rh / + /).

 Example. Patient N. (medical history N 6604) complained of bleeding and sore gums, bad breath. An objective examination of the oral cavity revealed hyperemia, swelling of the papillary and marginal gums. When probing, the interdental papillae bleed. The supra and subgingival tartar and pathological pocket up to 4 mm were determined.

 Diagnosis: exacerbation of chronic generalized periodontitis of moderate severity.

. В результате чего была получена десневая кровь, которая удлиненной пипеткой вносилась в пробирку с глюгициром и физ. р-ром в соотношении 1 4. Из пальца периферическую кровь брали скарификационным пером и капилляром и вносили в другую пробирку с глюгициром и физ. р-ром в соотношении 1 4. Обе пробирки центрифугировали 10 мин при 2000 об/мин, отделяли надосадочную жидкость и использовали ее для определения R-белков в десневой и периферической крови.Additional research methods: an excavator performed curettage of pathological gingival pockets in the region

. As a result, gingival blood was obtained, which was added with an elongated pipette into a test tube with glugicir and physical. r-rum in the ratio of 1 4. From the finger, peripheral blood was taken with a scarification pen and capillary and introduced into another test tube with glugicir and physical. p-rum in the ratio of 1 4. Both tubes were centrifuged for 10 min at 2000 rpm, the supernatant was separated and used to determine R-proteins in the gingival and peripheral blood.

 The research results are shown in the table.

Data analysis:
1) In the gingival blood of patients with periodontal tissue diseases of 100, an increased level of R-protein is detected (norm 11.6 12.6).

 2) Concomitant pathology does not affect the level of R-protein in the gingival blood, since in both groups of patients the average R-protein in the gingival blood is statistically significantly higher than the R-protein from the peripheral blood.

 3) Determining the level of R-protein in the gingival blood can be used as an immunological test for the diagnosis of periodontal disease.

 The advantage of the proposed method is: objectivity of the assessment, simplicity of technical performance, the minimum amount of material to be studied, the possibility of early diagnosis, the established production of reagents and their low cost. TTT1

Claims (1)

 A method for the diagnosis of periodontal tissue diseases, which consists in determining the protein in the biological fluid of the oral cavity, characterized in that they take blood from the gums and determine the R-protein and diagnose pathology at a level of more than 12.6.

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