RU2014154063A - METHODS FOR INCREASING YIELD RESISTANT TO 2,4-D AGRICULTURAL CROPS - Google Patents

METHODS FOR INCREASING YIELD RESISTANT TO 2,4-D AGRICULTURAL CROPS Download PDF

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RU2014154063A
RU2014154063A RU2014154063A RU2014154063A RU2014154063A RU 2014154063 A RU2014154063 A RU 2014154063A RU 2014154063 A RU2014154063 A RU 2014154063A RU 2014154063 A RU2014154063 A RU 2014154063A RU 2014154063 A RU2014154063 A RU 2014154063A
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herbicide
application
aryloxyalkanoate
aad
resistant crops
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Томас ХОФФМАН
Юньсин ЦУЙ
Малкольм ОБОРН
Дон М. ПАРКХЕРСТ
Барри УИГГИНЗ
Майкл ВЕРКАУТЕРЕН
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ДАУ АГРОСАЙЕНСИЗ ЭлЭлСи
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Abstract

1. Способ повышения урожайности резистентных к 2,4-D сельскохозяйственных культур, включающий обработку растений стимулирующим количеством гербицида, содержащего остаток арилоксиалканоата.2. Способ по п. 1, где резистентные к 2,4-D сельскохозяйственные культуры являются трансгенными растениями, трансформированными арилоксиалканоатдиоксигеназой (AAD).3. Способ по п. 2, где арилоксиалканоатдиоксигеназа (AAD) является AAD-12.4. Способ по п. 1, где гербицид, содержащий остаток арилоксиалканоата, является фенокси-гербицидом или феноксиуксусным гербицидом.5. Способ по п. 1, где гербицид, содержащий остаток арилоксиалканоата, является 2,4-D.6. Способ по п. 5, где 2,4-D содержит 2,4-D-холин или 2,4-D-диметиламин (DMA).7. Способ по п. 1, где обработку осуществляют по меньшей мере один раз при норме внесения 2,4-D, которую также применяют для борьбы с сорняками.8. Способ по п. 1, где обработку осуществляют дважды при норме внесения 2,4-D, которую также применяют для борьбы с сорняками.9. Способ по п. 8, где 2,4-D наносят в фазы роста V3 и R2 сои с устойчивостью к 2,4-D.10. Способ по п. 1, где обработку осуществляют по меньшей мере три раза при норме внесения 2,4-D, которую также применяют для борьбы с сорняками.11. Способ по п. 1, где резистентные к 2,4-D сельскохозяйственные культуры находятся в состоянии стресса.12. Способ по п. 1, где резистентные к 2,4-D сельскохозяйственные культуры для контроля сорняков также обрабатывают гербицидом, иным, чем 2,4-D.13. Способ по п. 12, где гербицид, иной, чем 2,4-D, является фосфорным гербицидом или арилоксифеноксипропионовым гербицидом.14. Способ по п. 13, где фосфорный гербицид содержит глифосат, глуфосинат, их производные или их комбинации.15. Способ по п. 13, где фосфорный гербицид находится в форме соли аммония, соли изопропиламмония, изопропиламиновой с1. A method of increasing the yield of 2,4-D-resistant crops, comprising treating the plants with a stimulating amount of a herbicide containing an aryloxyalkanoate residue. 2. The method of claim 1, wherein the 2,4-D resistant crops are transgenic plants transformed with aryloxyalkanoate dioxygenase (AAD). The method of claim 2, wherein the aryloxyalkanoate dioxygenase (AAD) is AAD-12.4. The method of claim 1, wherein the herbicide containing the aryloxyalkanoate residue is a phenoxy herbicide or phenoxyacetic herbicide. The method of claim 1, wherein the herbicide containing the aryloxyalkanoate residue is 2,4-D.6. The method of claim 5, wherein 2,4-D contains 2,4-D-choline or 2,4-D-dimethylamine (DMA). A method according to claim 1, wherein the treatment is carried out at least once at a rate of application of 2,4-D, which is also used for weed control. The method according to claim 1, wherein the treatment is carried out twice at a rate of application of 2,4-D, which is also used for weed control. The method of claim 8, wherein 2,4-D is applied to soybean growth phases V3 and R2 with resistance to 2,4-D.10. The method according to claim 1, wherein the treatment is carried out at least three times with a rate of application of 2,4-D, which is also used for weed control. The method of claim 1, wherein the 2,4-D resistant crops are under stress. The method of claim 1, wherein the 2,4-D resistant crops for weed control are also treated with a herbicide other than 2,4-D.13. The method of claim 12, wherein the herbicide other than 2,4-D is a phosphoric herbicide or an aryloxyphenoxypropionic herbicide. The method of claim 13, wherein the phosphoric herbicide comprises glyphosate, glufosinate, derivatives thereof, or combinations thereof. The method of claim 13, wherein the phosphoric herbicide is in the form of an ammonium salt, an isopropylammonium salt, an isopropylamine salt with

Claims (32)

1. Способ повышения урожайности резистентных к 2,4-D сельскохозяйственных культур, включающий обработку растений стимулирующим количеством гербицида, содержащего остаток арилоксиалканоата.1. A method of increasing the yield of 2,4-D-resistant crops, comprising treating the plants with a stimulating amount of a herbicide containing an aryloxyalkanoate residue. 2. Способ по п. 1, где резистентные к 2,4-D сельскохозяйственные культуры являются трансгенными растениями, трансформированными арилоксиалканоатдиоксигеназой (AAD).2. The method of claim 1, wherein the 2,4-D resistant crops are transgenic plants transformed with aryloxyalkanoate dioxygenase (AAD). 3. Способ по п. 2, где арилоксиалканоатдиоксигеназа (AAD) является AAD-12.3. The method of claim 2, wherein the aryloxyalkanoate dioxygenase (AAD) is AAD-12. 4. Способ по п. 1, где гербицид, содержащий остаток арилоксиалканоата, является фенокси-гербицидом или феноксиуксусным гербицидом.4. The method of claim 1, wherein the herbicide containing the aryloxyalkanoate residue is a phenoxy herbicide or phenoxyacetic herbicide. 5. Способ по п. 1, где гербицид, содержащий остаток арилоксиалканоата, является 2,4-D.5. The method of claim 1, wherein the herbicide containing the aryloxyalkanoate residue is 2,4-D. 6. Способ по п. 5, где 2,4-D содержит 2,4-D-холин или 2,4-D-диметиламин (DMA).6. The method of claim 5, wherein 2,4-D contains 2,4-D-choline or 2,4-D-dimethylamine (DMA). 7. Способ по п. 1, где обработку осуществляют по меньшей мере один раз при норме внесения 2,4-D, которую также применяют для борьбы с сорняками.7. The method according to p. 1, where the processing is carried out at least once with a rate of application of 2,4-D, which is also used for weed control. 8. Способ по п. 1, где обработку осуществляют дважды при норме внесения 2,4-D, которую также применяют для борьбы с сорняками.8. The method according to p. 1, where the treatment is carried out twice at a rate of application of 2,4-D, which is also used to control weeds. 9. Способ по п. 8, где 2,4-D наносят в фазы роста V3 и R2 сои с устойчивостью к 2,4-D.9. The method of claim 8, wherein 2,4-D is applied to soybean growth phases V3 and R2 with resistance to 2,4-D. 10. Способ по п. 1, где обработку осуществляют по меньшей мере три раза при норме внесения 2,4-D, которую также применяют для борьбы с сорняками.10. The method according to p. 1, where the processing is carried out at least three times at a rate of application of 2,4-D, which is also used for weed control. 11. Способ по п. 1, где резистентные к 2,4-D сельскохозяйственные культуры находятся в состоянии стресса.11. The method of claim 1, wherein the 2,4-D resistant crops are under stress. 12. Способ по п. 1, где резистентные к 2,4-D сельскохозяйственные культуры для контроля сорняков также обрабатывают гербицидом, иным, чем 2,4-D.12. The method of claim 1, wherein the 2,4-D resistant crops for weed control are also treated with a herbicide other than 2,4-D. 13. Способ по п. 12, где гербицид, иной, чем 2,4-D, является фосфорным гербицидом или арилоксифеноксипропионовым гербицидом.13. The method of claim 12, wherein the herbicide other than 2,4-D is a phosphoric herbicide or an aryloxyphenoxypropionic herbicide. 14. Способ по п. 13, где фосфорный гербицид содержит глифосат, глуфосинат, их производные или их комбинации.14. The method of claim 13, wherein the phosphoric herbicide comprises glyphosate, glufosinate, derivatives thereof, or combinations thereof. 15. Способ по п. 13, где фосфорный гербицид находится в форме соли аммония, соли изопропиламмония, изопропиламиновой соли или соли калия.15. The method of claim 13, wherein the phosphoric herbicide is in the form of an ammonium salt, an isopropylammonium salt, an isopropylamine salt, or a potassium salt. 16. Способ по п. 13, где арилоксифеноксипропионовый гербицид содержит хлоразифоп, феноксапроп, флуазифоп, галоксифоп, квизалофоп, их производные или их комбинации.16. The method of claim 13, wherein the aryloxyphenoxypropionic herbicide comprises chlorazifop, fenoxaprop, fluazifop, haloxifop, quizalofop, their derivatives, or combinations thereof. 17. Способ по п. 1, где резистентные к 2,4-D сельскохозяйственные культуры обрабатывают по меньшей мере один раз при норме внесения от 25 г кэ/га до 5000 г кэ/га 2,4-D.17. The method of claim 1, wherein the 2,4-D resistant crops are treated at least once at a rate of application of 25 g ke / ha to 5,000 g ke / ha 2,4-D. 18. Способ по п. 1, где резистентные к 2,4-D сельскохозяйственные культуры обрабатывают по меньшей мере один раз при норме внесения от 100 г кэ/га до 2500 г кэ/га 2,4-D.18. The method according to p. 1, where resistant to 2,4-D crops are treated at least once at a rate of application of from 100 g ke / ha to 2500 g ke / ha of 2,4-D. 19. Способ по п. 1, где гербицид, содержащий остаток арилоксиалканоата, попадает в резистентные к 2,4-D сельскохозяйственные культуры посредством поглощения корнями.19. The method of claim 1, wherein the herbicide containing the aryloxyalkanoate residue enters 2,4-D-resistant crops through root uptake. 20. Способ по п. 13, где фосфорный гербицид попадает в резистентные к 2,4-D сельскохозяйственные культуры посредством поглощения корнями.20. The method of claim 13, wherein the phosphoric herbicide enters 2,4-D resistant crops through root uptake. 21. Способ по п. 13, где арилоксифеноксипропионовый гербицид попадает в резистентные к 2,4-D сельскохозяйственные культуры посредством поглощения корнями.21. The method of claim 13, wherein the aryloxyphenoxypropionic herbicide enters 2,4-D resistant crops through root uptake. 22. Способ по п. 2, где трансгенные растения, трансформированные с использованием арилоксиалканоатдиоксигеназы (AAD), выбирают из хлопка, сои и канолы.22. The method of claim 2, wherein the transgenic plants transformed using aryloxyalkanoate dioxygenase (AAD) are selected from cotton, soy, and canola. 23. Способ повышения урожайности резистентных к 2,4-D сельскохозяйственных культур, включающий23. A method of increasing the yield of 2,4-D resistant crops, comprising (a) трансформацию растительных клеток молекулой нуклеиновой кислоты, содержащей нуклеотидную последовательность, кодирующую арилоксиалканоатдиоксигеназу (AAD);(a) transforming plant cells with a nucleic acid molecule containing a nucleotide sequence encoding an aryloxyalkanoate dioxygenase (AAD); (b) селекцию трансформированных клеток;(b) selection of transformed cells; (c) регенерацию растений из трансформированных клеток; и(c) plant regeneration from transformed cells; and (d) обработку растений стимулирующим количеством гербицида, содержащего остаток арилоксиалканоата.(d) treating the plants with a stimulating amount of a herbicide containing an aryloxy alkanoate residue. 24. Способ по п. 23, где арилоксиалканоатдиоксигеназа (AAD) является AAD-12.24. The method of claim 23, wherein the aryloxyalkanoate dioxygenase (AAD) is AAD-12. 25. Способ по п. 23, где молекула нуклеиновой кислоты содержит селективный маркер, не являющийся арилоксиалканоатдиоксигеназой (AAD).25. The method of claim 23, wherein the nucleic acid molecule comprises a non-aryloxyalkanoate dioxygenase (AAD) selective marker. 26. Способ по п. 25, где селективный маркер является геном фосфинотрицинацетилтрансферазы (pat) или геном резистентности к биалафосу (bar).26. The method of claim 25, wherein the selective marker is a phosphinotricin acetyltransferase (pat) gene or a bialaphos resistance gene (bar). 27. Способ по п. 23, где молекула нуклеиновой кислоты оптимизирована для растений.27. The method of claim 23, wherein the nucleic acid molecule is optimized for plants. 28. Применение 2,4-D в производстве трансгенных растений с резистентностью к 2,4-D с повышенной урожайностью по сравнению с их нетрансгенными родительскими растениями.28. The use of 2,4-D in the production of transgenic plants with resistance to 2,4-D with increased yield compared to their non-transgenic parent plants. 29. Применение по п. 28, где 2,4-D наносят по меньшей мере один раз при норме внесения от 25 г кэ/га до 5000 г кэ/га 2,4-D.29. The application of claim 28, wherein 2,4-D is applied at least once at a rate of application of 25 g ke / ha to 5,000 g ke / ha 2,4-D. 30. Применение по п. 28, где 2,4-D наносят по меньшей мере один раз при норме внесения от 100 г кэ/га до 2500 г кэ/га 2,4-D.30. The application of claim 28, wherein 2,4-D is applied at least once at a rate of application of from 100 g ke / ha to 2500 g ke / ha of 2,4-D. 31. Применение по п. 28, где 2,4-D содержит 2,4-D-холин или 2,4-D-диметиламин (DMA).31. The application of claim 28, wherein 2,4-D contains 2,4-D-choline or 2,4-D-dimethylamine (DMA). 32. Применение по п. 28, где резистентные к 2,4-D сельскохозяйственные культуры обрабатывают 2,4-D по меньшей мере два раза перед цветением. 32. The application of claim 28, wherein the 2,4-D resistant crops are treated with 2,4-D at least two times before flowering.
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