RU2013130607A - METHOD FOR DETECTING MYCOBACTERIA TUBERCULOSIS GENETIC CLUSTER Beijing B0 / W148 - Google Patents
METHOD FOR DETECTING MYCOBACTERIA TUBERCULOSIS GENETIC CLUSTER Beijing B0 / W148 Download PDFInfo
- Publication number
- RU2013130607A RU2013130607A RU2013130607/10A RU2013130607A RU2013130607A RU 2013130607 A RU2013130607 A RU 2013130607A RU 2013130607/10 A RU2013130607/10 A RU 2013130607/10A RU 2013130607 A RU2013130607 A RU 2013130607A RU 2013130607 A RU2013130607 A RU 2013130607A
- Authority
- RU
- Russia
- Prior art keywords
- beijing
- sec
- strains
- cluster
- mycobacteria tuberculosis
- Prior art date
Links
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Способ идентификации микобактерий туберкулеза кластера Beijing B0/W148, отличающийся тем, что применяют полимеразную цепную реакцию для амплификации фрагментов ДНК М. tuberculosis с подобранными праймерами P1, P2 и РЗ, последовательности которых представлены SEQ ID NO 1, 2, 3, соответственно, в режиме амплификации 98°С - 10 мин., 30 циклов: 98°С - 30 сек., 64°С - 30 сек., 72°С - 50 сек., 72°С - 10 мин., 4°С, разделение продуктов амплификации проводят электрофорезом в 2% агарозном геле с получением фрагмента размером 1021 пн для штаммов Beijing B0/W148 или размером 320 пн - для штаммов, не относящихся к данному семейству.A method for identifying mycobacterium tuberculosis of the Beijing B0 / W148 cluster, characterized in that a polymerase chain reaction is used to amplify M. tuberculosis DNA fragments with selected primers P1, P2 and RE, the sequences of which are represented by SEQ ID NO 1, 2, 3, respectively, in the mode amplification 98 ° С - 10 min., 30 cycles: 98 ° С - 30 sec., 64 ° С - 30 sec., 72 ° С - 50 sec., 72 ° С - 10 min., 4 ° С, product separation amplifications are carried out by electrophoresis in a 2% agarose gel to obtain a fragment of 1021 bp for strains of Beijing B0 / W148 or 320 bp for strains without who are members of this family.
Claims (1)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
RU2013130607/10A RU2551764C2 (en) | 2013-07-04 | 2013-07-04 | METHOD FOR DETECTING TUBERCULOUSIS MYCOBACTERIA OF GENETIC CLUSTER Beijing B0/W148 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
RU2013130607/10A RU2551764C2 (en) | 2013-07-04 | 2013-07-04 | METHOD FOR DETECTING TUBERCULOUSIS MYCOBACTERIA OF GENETIC CLUSTER Beijing B0/W148 |
Publications (2)
Publication Number | Publication Date |
---|---|
RU2013130607A true RU2013130607A (en) | 2015-01-10 |
RU2551764C2 RU2551764C2 (en) | 2015-05-27 |
Family
ID=53279064
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
RU2013130607/10A RU2551764C2 (en) | 2013-07-04 | 2013-07-04 | METHOD FOR DETECTING TUBERCULOUSIS MYCOBACTERIA OF GENETIC CLUSTER Beijing B0/W148 |
Country Status (1)
Country | Link |
---|---|
RU (1) | RU2551764C2 (en) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
RU2684314C2 (en) * | 2017-06-30 | 2019-04-05 | федеральное государственное бюджетное учреждение "Санкт-Петербургский научно-исследовательский институт фтизиопульмонологии" Министерства здравоохранения Российской Федерации | Method of identification of mycobacteria of tuberculosis of the beijing genotype b0 cluster in real time format |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
RU2405836C2 (en) * | 2008-05-12 | 2010-12-10 | Федеральное государственное учреждение "Санкт-Петербургский научно-исследовательский институт фтизиопульмонологии Федерального агентства по высокотехнологичной медицинской помощи" | Method for mycobacterium tuberculosis beijing genotype detection |
-
2013
- 2013-07-04 RU RU2013130607/10A patent/RU2551764C2/en not_active IP Right Cessation
Also Published As
Publication number | Publication date |
---|---|
RU2551764C2 (en) | 2015-05-27 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
EP4303314A3 (en) | Polypeptide tagged nucleotides and use thereof in nucleic acid sequencing by nanopore detection | |
MX2017005159A (en) | Compositions and methods for detecting an rna virus. | |
MX2017003228A (en) | Methods for pathogen detection and disease management on meats, plants, or plant parts. | |
WO2015081229A3 (en) | Selective amplification of nucleic acid sequences | |
SA516371691B1 (en) | Methods and compositions for dna profiling | |
EP3216867A4 (en) | Method for modifying genome sequence to introduce specific mutation to targeted dna sequence by base-removal reaction, and molecular complex used therein | |
WO2014116729A3 (en) | Haplotying of hla loci with ultra-deep shotgun sequencing | |
MX2016007541A (en) | Modified nucleic acid molecules and uses thereof. | |
BR112016016916A2 (en) | hereditary angiodema treatment method (hae) | |
WO2018170614A8 (en) | New technique for genomic large fragment direct cloning and dna multi-molecular assembly | |
WO2015058008A3 (en) | Enhanced nucleic acid identification and detection | |
WO2014143714A3 (en) | Methods for one step nucleic acid amplification of non-eluted samples | |
SG10201806000YA (en) | Primer set for pcr for bacterial dna amplification, kit for detection and/or identification of bacterial species, and, method of detection and/or identification of bacterial species | |
BR112016029907A2 (en) | genetically adapted enrichment and polymerase chain reaction for rapid detection of microorganisms ". | |
GB2519465A (en) | Solid matrix for one step nucleic acid amplification | |
EA038921B9 (en) | Detection of target nucleic acid and variants thereof | |
MY184632A (en) | A method of identifying parentage in freshwater prawn macrobrachium rosenbergii | |
MX2016006602A (en) | Nucleic Acid Amplification. | |
CA2869033C (en) | Nucleic acids for nucleic acid amplification | |
MX2017003473A (en) | Hybrid multi-step nucleic acid amplification. | |
RU2013130607A (en) | METHOD FOR DETECTING MYCOBACTERIA TUBERCULOSIS GENETIC CLUSTER Beijing B0 / W148 | |
MY182547A (en) | Polymerase chain reaction detection system | |
WO2014136124A3 (en) | Multiplex real time pcr testing kit for the simultaneous detection of hepatitis virus | |
WO2012151599A3 (en) | Lamp method for detecting erwinia amylovora | |
EA202190758A1 (en) | COMPOSITION FOR INDUCTION OF CELL DEATH HAVING MUTATED GENE, AND METHOD FOR INDUCTION OF CELL DEATH HAVING MUTATED GENE USING COMPOSITION |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
MM4A | The patent is invalid due to non-payment of fees |
Effective date: 20150705 |