RU2013104059A - METHOD FOR PRODUCING BIOLOGICAL PRODUCT FOR CLEANING AND RESTORING FERTILITY OF SOIL SOIL CONTAMINATED WITH OIL PRODUCTS - Google Patents

METHOD FOR PRODUCING BIOLOGICAL PRODUCT FOR CLEANING AND RESTORING FERTILITY OF SOIL SOIL CONTAMINATED WITH OIL PRODUCTS Download PDF

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RU2013104059A
RU2013104059A RU2013104059/13A RU2013104059A RU2013104059A RU 2013104059 A RU2013104059 A RU 2013104059A RU 2013104059/13 A RU2013104059/13 A RU 2013104059/13A RU 2013104059 A RU2013104059 A RU 2013104059A RU 2013104059 A RU2013104059 A RU 2013104059A
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Russia
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titer
mixture
strains
soil
cleaning
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RU2013104059/13A
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Russian (ru)
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RU2529735C1 (en
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Наталья Александровна Галкина
Валентин Юрьевич Каплунов
Юрий Валентинович Каплунов
Евгений Юрьевич Глоба
Евгений Аркадьевич Галкин
Ирина Валерьяновна Катаева
Владимир Николаевич Шафран
Ольга Александровна Назаренко
Константин Александрович Вяткин
Марина Владимировна Мальцева
Евгений Александрович Ковальчук
Татьяна Александровна Кузнецова
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Общество с ограниченной ответственностью "Уралэкоресурс" ООО "Уралэкоресурс"
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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Processing Of Solid Wastes (AREA)
  • Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)

Abstract

Способ получения биопрепарата для очистки и восстановления плодородия почвогрунтов, загрязненных нефтепродуктами, на основе наполнителя и бактериальных культур, отличающийся тем, что готовят смесь из жидких бактериальных культур, в качестве которых берут штаммы Pseudomonas fluorescens ВКГ RCAM00538 с титром 10, КОА-4 Pseudomonas fluorescens ND-610 ВНИИСХМ с титром 10, Azotobacter chroococcum АИН RCAM00539 с титром 10в соотношении соответственно 3:1,5:0,5-3:2:1, и осуществляют обработку отходов обогащения бурого угля путем добавления к ним полученной жидкой смеси штаммов с последующим перемешиванием и высушиванием приготовленной смеси в течение не менее 2,5 суток при комнатной температуре, при этом отходы обогащения бурого угля и предварительно подготовленную смесь штаммов берут соответственно в соотношении, мас.%: 40-50:50-60.A method of obtaining a biological product for cleaning and restoring the fertility of soil contaminated with oil products based on filler and bacterial cultures, characterized in that a mixture of liquid bacterial cultures is prepared, which are taken as strains of Pseudomonas fluorescens VKH RCAM00538 with a titer of 10, KOA-4 Pseudomonas fluorescens ND -610 VNIISCHM with a titer of 10, Azotobacter chroococcum AIN RCAM00539 with a titer of 10 in a ratio of 3: 1.5: 0.5-3: 2: 1, respectively, and brown coal is treated by adding the resulting liquid mixture of strains, followed by changing suturing and drying the prepared mixture for at least 2.5 days at room temperature, while the waste from brown coal enrichment and the previously prepared mixture of strains are taken respectively in the ratio, wt.%: 40-50: 50-60.

Claims (1)

Способ получения биопрепарата для очистки и восстановления плодородия почвогрунтов, загрязненных нефтепродуктами, на основе наполнителя и бактериальных культур, отличающийся тем, что готовят смесь из жидких бактериальных культур, в качестве которых берут штаммы Pseudomonas fluorescens ВКГ RCAM00538 с титром 10-13, КОА-4 Pseudomonas fluorescens ND-610 ВНИИСХМ с титром 10-10, Azotobacter chroococcum АИН RCAM00539 с титром 10-12 в соотношении соответственно 3:1,5:0,5-3:2:1, и осуществляют обработку отходов обогащения бурого угля путем добавления к ним полученной жидкой смеси штаммов с последующим перемешиванием и высушиванием приготовленной смеси в течение не менее 2,5 суток при комнатной температуре, при этом отходы обогащения бурого угля и предварительно подготовленную смесь штаммов берут соответственно в соотношении, мас.%: 40-50:50-60. A method of obtaining a biological product for cleaning and restoring fertility of soil contaminated with oil products, based on filler and bacterial cultures, characterized in that a mixture of liquid bacterial cultures is prepared, which are taken as strains of Pseudomonas fluorescens VKH RCAM00538 with a titer of 10 -13 , KOA-4 Pseudomonasas fluorescens ND-610 VNIISCHM with a titer of 10 -10 , Azotobacter chroococcum AIN RCAM00539 with a titer of 10 -12 in a ratio of 3: 1.5: 0.5-3: 2: 1, respectively, and brown coal wastes are processed by adding to them the resulting liquid mixture of strains followed by m stirring and drying the prepared mixture for at least 2.5 days at room temperature, while the waste from brown coal enrichment and the previously prepared mixture of strains are taken respectively in the ratio, wt.%: 40-50: 50-60.
RU2013104059/13A 2013-01-30 2013-01-30 Method of producing biopreparation for cleaning and restoring fertility of soil contaminated with petroleum products RU2529735C1 (en)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2688282C1 (en) 2018-07-23 2019-05-21 Юрий Борисович Толкачников Method for remediation of contaminated land
RU2705290C1 (en) * 2019-05-13 2019-11-06 Федеральное государственное бюджетное учреждение науки СИБИРСКИЙ ИНСТИТУТ ФИЗИОЛОГИИ И БИОХИМИИ РАСТЕНИЙ СИБИРСКОГО ОТДЕЛЕНИЯ РОССИЙСКОЙ АКАДЕМИИ НАУК Microbial preparation for bioremediation of soil contaminated with oil and oil products

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* Cited by examiner, † Cited by third party
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RU2176164C2 (en) * 1999-06-30 2001-11-27 Чекасина Елизавета Васильевна Method of biological remediation of soil polluted with oil
RU2191643C1 (en) * 2001-07-09 2002-10-27 Закрытое акционерное общество "Полиинформ" Method of removing crude oil and petroleum product impurities from ground
RU2290270C1 (en) * 2005-07-27 2006-12-27 Федеральное государственное унитарное предприятие Межотраслевой научно-исследовательский институт экологии топливно-энергетического комплекса (ФГУП МНИИЭКО ТЭК) Method of biological remediation of oil-polluted lands
CN101603018A (en) * 2009-07-07 2009-12-16 南开大学 Degraded oil, restoring petroleum polluted soil ecology bacteria preparation and preparation method thereof

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