RU2011102403A - DEVICE AND METHOD OF BIOTESTING TOXICITY OF WATERS AND AQUEOUS SOLUTIONS - Google Patents
DEVICE AND METHOD OF BIOTESTING TOXICITY OF WATERS AND AQUEOUS SOLUTIONS Download PDFInfo
- Publication number
- RU2011102403A RU2011102403A RU2011102403/15A RU2011102403A RU2011102403A RU 2011102403 A RU2011102403 A RU 2011102403A RU 2011102403/15 A RU2011102403/15 A RU 2011102403/15A RU 2011102403 A RU2011102403 A RU 2011102403A RU 2011102403 A RU2011102403 A RU 2011102403A
- Authority
- RU
- Russia
- Prior art keywords
- light
- delayed fluorescence
- low
- conditions
- intensity
- Prior art date
Links
- 239000007864 aqueous solution Substances 0.000 title claims abstract 3
- 238000000034 method Methods 0.000 title claims abstract 3
- 230000001988 toxicity Effects 0.000 title claims abstract 3
- 231100000419 toxicity Toxicity 0.000 title claims abstract 3
- 239000003643 water by type Substances 0.000 title 1
- 230000003111 delayed effect Effects 0.000 claims abstract 6
- 229930002875 chlorophyll Natural products 0.000 claims abstract 4
- 235000019804 chlorophyll Nutrition 0.000 claims abstract 4
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 claims abstract 4
- 230000003287 optical effect Effects 0.000 claims abstract 4
- 206010021033 Hypomenorrhoea Diseases 0.000 claims abstract 2
- 230000005284 excitation Effects 0.000 claims abstract 2
- 230000006698 induction Effects 0.000 claims abstract 2
- 230000001678 irradiating effect Effects 0.000 claims abstract 2
- 238000004020 luminiscence type Methods 0.000 claims abstract 2
- 238000005259 measurement Methods 0.000 claims abstract 2
- 238000001228 spectrum Methods 0.000 claims abstract 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract 2
Landscapes
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
1. Устройство для регистрации замедленной флуоресценции хлорофилла растительных объектов, включающее светогерметичный корпус, источник возбуждающего света, модулятор света, оптический блок, держатель для объекта и двигатель, отличающееся тем, что в оптическом блоке в качестве источника возбуждающего света использованы светодиоды синего спектра излучения, и перед фотоприемником установлен красный светофильтр, а модулятор света выполнен в виде электронного блока. ! 2. Устройство по п.1, отличающееся тем, что держатель для объекта выполнен в виде дисковой кассеты, в которой кюветы располагаются по кругу. ! 3. Способ биотестирования токсичности вод и водных растворов, включающий облучение хлорофиллсодержащего растительного тест-организма, помещенного в анализируемую пробу, сначала импульсным светом высокой интенсивности, а затем низкой, и измерение величины отношения интенсивностей его миллисекундной замедленной флуоресценции в индукционных максимумах в данных световых условиях, отличающийся тем, что условия низкого светового облучения создают за счет сокращения длительности световых периодов возбуждения свечения при одновременном увеличении темновых интервалов между ними, а условия высокого светового облучения обеспечивают через увеличение соотношения длительности световых периодов к темновым, при этом измерение интенсивности замедленной флуоресценции тест-организма в режиме высокого света производят в начале каждой кривой затухания, а в режиме низкого - в конце. 1. A device for recording the delayed fluorescence of chlorophyll of plant objects, including a light-tight housing, an exciting light source, a light modulator, an optical unit, an object holder and an engine, characterized in that the blue light spectrum LEDs are used as an exciting light source in the optical unit, and A red filter is installed in front of the photodetector, and the light modulator is made in the form of an electronic unit. ! 2. The device according to claim 1, characterized in that the holder for the object is made in the form of a disk cassette in which the cuvettes are arranged in a circle. ! 3. A method for biotesting the toxicity of water and aqueous solutions, including irradiating a chlorophyll-containing plant test organism, placed in the analyzed sample, first with high-intensity pulsed light and then low, and measuring the intensity ratio of its millisecond delayed fluorescence at induction maxima under given light conditions, characterized in that the conditions of low light exposure are created by reducing the duration of light periods of excitation of luminescence while velichenii dark intervals between them, and a high light irradiation conditions ensure duration by increasing the ratio of light to the dark periods, the measurement of delayed fluorescence intensity of the test organism to produce a high beam mode at the beginning of each decay curve, and low regime - in the end.
Claims (3)
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RU2011102403/15A RU2482474C2 (en) | 2011-01-21 | 2011-01-21 | Method for biotesting toxicity of water and aqueous solutions |
| EA201101386A EA021097B1 (en) | 2011-01-21 | 2011-10-25 | Method for biotesting toxicity of water and aqueous solutions |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RU2011102403/15A RU2482474C2 (en) | 2011-01-21 | 2011-01-21 | Method for biotesting toxicity of water and aqueous solutions |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| RU2011102403A true RU2011102403A (en) | 2012-07-27 |
| RU2482474C2 RU2482474C2 (en) | 2013-05-20 |
Family
ID=46614808
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2011102403/15A RU2482474C2 (en) | 2011-01-21 | 2011-01-21 | Method for biotesting toxicity of water and aqueous solutions |
Country Status (2)
| Country | Link |
|---|---|
| EA (1) | EA021097B1 (en) |
| RU (1) | RU2482474C2 (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106248882B (en) * | 2016-08-19 | 2018-04-17 | 河北神通光电科技有限公司 | The method for obtaining the red blue spectrum ratios of plant illumination LED |
| RU2732203C1 (en) * | 2019-12-26 | 2020-09-14 | федеральное государственное автономное образовательное учреждение высшего образования «Национальный исследовательский Томский политехнический университет» | Method of determining concentration of cells in suspension of microalgae |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3873273A (en) * | 1973-10-15 | 1975-03-25 | Hycel Inc | Automatic chemical testing system |
| SU492805A1 (en) * | 1974-03-20 | 1975-11-25 | Всесоюзный научно-исследовательский институт химических средств защиты растений | Method for determining herbicidal activity |
| RU2069851C1 (en) * | 1992-07-15 | 1996-11-27 | Григорьев Юрий Сергеевич | Process of detection of content of phytotoxic substances |
| RU2065722C1 (en) * | 1992-09-17 | 1996-08-27 | Раменское приборостроительное конструкторское бюро | Device for evaluation of eye fundus blood supply |
| ES2259277T3 (en) * | 1993-09-24 | 2006-10-01 | Abbott Laboratories | METHOD OF OPERATING AN AUTOMATIC ANALYTICAL SYSTEM OF CONTINUOUS RANDOM ACCESS ABLE TO SIMULTANEOUSLY MULTIPLE TESTS IN A PLURALITY OF LIQUID SAMPLES. |
| HRP20031022A2 (en) * | 2001-05-09 | 2005-10-31 | Axis Shield Asa | Assay system |
| WO2003023379A1 (en) * | 2001-09-12 | 2003-03-20 | Apprise Technologies, Inc. | Multichannel fluorosensor |
| RU2349355C2 (en) * | 2006-11-07 | 2009-03-20 | Андрей Андреевич Ворона | Physiotherapeutic device for light-beam therapy |
| WO2009111834A1 (en) * | 2008-03-14 | 2009-09-17 | Runcie John W | Automated shutter for dark acclimating samples |
-
2011
- 2011-01-21 RU RU2011102403/15A patent/RU2482474C2/en active
- 2011-10-25 EA EA201101386A patent/EA021097B1/en not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| EA021097B1 (en) | 2015-04-30 |
| RU2482474C2 (en) | 2013-05-20 |
| EA201101386A1 (en) | 2012-07-30 |
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