RU2004110632A - METHOD FOR ACCUMULATING A LISTERIOUS MICROBE (LISTERIA MONOCYTOGENES) - Google Patents

METHOD FOR ACCUMULATING A LISTERIOUS MICROBE (LISTERIA MONOCYTOGENES) Download PDF

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Publication number
RU2004110632A
RU2004110632A RU2004110632/13A RU2004110632A RU2004110632A RU 2004110632 A RU2004110632 A RU 2004110632A RU 2004110632/13 A RU2004110632/13 A RU 2004110632/13A RU 2004110632 A RU2004110632 A RU 2004110632A RU 2004110632 A RU2004110632 A RU 2004110632A
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RU
Russia
Prior art keywords
solution
microbe
accumulating
listeria monocytogenes
listerious
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RU2004110632/13A
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Russian (ru)
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RU2268307C1 (en
Inventor
Валери Евгеньевна Терехова (RU)
Валерия Евгеньевна Терехова
Нина Александровна Айздайчер (RU)
Нина Александровна Айздайчер
Любовь Степановна Бузолева (RU)
Любовь Степановна Бузолева
Ангелина Михайловна Кривошеева (RU)
Ангелина Михайловна Кривошеева
Георгий Павлович Сомов (RU)
Георгий Павлович Сомов
Original Assignee
Научно-исследовательский институт эпидемиологии и микробиологии Сибирского отделени Российской Академии Медицинских наук (RU)
Научно-исследовательский институт эпидемиологии и микробиологии Сибирского отделения Российской Академии Медицинских наук
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Priority to RU2004110632/13A priority Critical patent/RU2268307C1/en
Publication of RU2004110632A publication Critical patent/RU2004110632A/en
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Способ накопления листериозного микроба (Listeria monocytogenes) включающий культивирование листерий в среде обогащения, отличающийся тем, что накопление бактерий проводят при температуре 18-20°С, а среда обогащения содержит растворитель и питательную основу, причем в качестве растворителя используют стерильную морскую воду, а в качестве питательной основы минеральные соли и при следующем соотношении компонентов в маточных растворах, мг/л дистиллированной воды:A method of accumulating listeriosis microbe (Listeria monocytogenes) comprising culturing listeria in an enrichment medium, characterized in that the accumulation of bacteria is carried out at a temperature of 18-20 ° C, and the enrichment medium contains a solvent and a nutrient base, and sterile sea water is used as the solvent, and as a nutrient base, mineral salts and in the following ratio of components in the mother liquor, mg / l of distilled water: Раствор №1:Solution No. 1: NaH2PO4 - 10NaH 2 PO 4 - 10 NaNO3 - 150NaNO 3 - 150 Раствор №2:Solution No. 2: Na2SiO3·9Н2О - 30-60Na 2 SiO 3 · 9H 2 O - 30-60 Раствор №3:Solution No. 3: CuSO4·5H2O - 0,0196CuSO 4 · 5H 2 O - 0.0196 ZnSO4·7H2O - 0,044ZnSO 4 · 7H 2 O - 0.044 CoCl2·6Н2О - 0,020CoCl 2 · 6H 2 O - 0.020 MnCl2·4H2O - 0,360MnCl 2 · 4H 2 O - 0.360 Na2MoO4·2Н2О - 0,0126Na 2 MoO 4 · 2H 2 O - 0.0126 Раствор №4:Solution No. 4: Na2EDTA - 6,8Na 2 EDTA - 6.8 Раствор №5:Solution No. 5: FeCl3·6H2O - 5,3FeCl 3 · 6H 2 O - 5.3 причем 1 л среды обогащения состоит из маточных растворов в следующем количестве:moreover, 1 l of enrichment medium consists of mother liquors in the following quantity: Раствор №1 - 0,5-2 млSolution No. 1 - 0.5-2 ml Раствор №2 - 0,5-2 млSolution No. 2 - 0.5-2 ml Раствор №3 - 0,5-2 млSolution No. 3 - 0.5-2 ml Раствор №4 - 0,5-2 млSolution No. 4 - 0.5-2 ml Раствор №5 - 0,5-2 мл,Solution No. 5 - 0.5-2 ml, Стерильная морская вода Остальное.Sterile Sea Water Else.
RU2004110632/13A 2004-04-07 2004-04-07 Method for accumulation of listeriosis microorganism (listeria monocytogenes) RU2268307C1 (en)

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RU2004110632/13A RU2268307C1 (en) 2004-04-07 2004-04-07 Method for accumulation of listeriosis microorganism (listeria monocytogenes)

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RU2004110632/13A RU2268307C1 (en) 2004-04-07 2004-04-07 Method for accumulation of listeriosis microorganism (listeria monocytogenes)

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RU2004110632A true RU2004110632A (en) 2005-10-10
RU2268307C1 RU2268307C1 (en) 2006-01-20

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2444567C1 (en) * 2011-02-02 2012-03-10 Учреждение Российской академии медицинских наук Научно-исследовательский институт эпидемиологии и микробиологии Сибирского отделения РАМН NUTRITIVE MEDIUM FOR DETERMINATION OF Listeria GENUS BACTERIA LECITHINASE ACTIVITY

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