RU2002117336A - METHOD FOR ACCUMULATING A LISTERIOUS MICROBE (LISTERIA MONOCYTOGENES) - Google Patents

METHOD FOR ACCUMULATING A LISTERIOUS MICROBE (LISTERIA MONOCYTOGENES)

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Publication number
RU2002117336A
RU2002117336A RU2002117336/13A RU2002117336A RU2002117336A RU 2002117336 A RU2002117336 A RU 2002117336A RU 2002117336/13 A RU2002117336/13 A RU 2002117336/13A RU 2002117336 A RU2002117336 A RU 2002117336A RU 2002117336 A RU2002117336 A RU 2002117336A
Authority
RU
Russia
Prior art keywords
solution
microbe
listeria monocytogenes
listerious
accumulating
Prior art date
Application number
RU2002117336/13A
Other languages
Russian (ru)
Inventor
Валерия Евгеньевна Терехова
Нина Александровна Айздайчер
Любовь Степановна Бузолева
Ангелина Михаиловна Кривошеева
Георгий Павлович Сомов
Original Assignee
Научно-исследовательский институт эпидемиологии и микробиологии СО РАМН
Filing date
Publication date
Application filed by Научно-исследовательский институт эпидемиологии и микробиологии СО РАМН filed Critical Научно-исследовательский институт эпидемиологии и микробиологии СО РАМН
Publication of RU2002117336A publication Critical patent/RU2002117336A/en

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Claims (1)

Способ накопления листериозного микроба Listeria monocytogenes, включающий культивирование листерий в среде обогащения, отличающийся тем, что накопление бактерий проводят при температуре 18-20°С, а среда обогащения содержит растворитель и питательную основу, причем в качестве растворителя используют морскую воду, а в качестве питательной основы минеральные соли и при следующем соотношении компонентов в маточных растворах, мг/л:The method of accumulation of Listeria monocytogenes listeriosis microbe, including the cultivation of Listeria in an enrichment medium, characterized in that the accumulation of bacteria is carried out at a temperature of 18-20 ° C, and the enrichment medium contains a solvent and a nutrient base, and seawater is used as a solvent, and nutrient mineral salts and the following ratio of components in mother liquors, mg / l: Раствор №1:Solution No. 1: NaH2PO4 10NaH 2 PO 4 10 NaNO3 150NaNO 3 150 Раствор №2:Solution No. 2: Na2SiO3 × 9H2O 30-60Na 2 SiO 3 × 9H 2 O 30-60 Раствор №3:Solution No. 3: CuSO4 × 5H2O 0,0196CuSO 4 × 5H 2 O 0.0196 ZnSO4 × 7H2O 0,044ZnSO 4 × 7H 2 O 0.044 CoCl2 × 6H2O 0,020CoCl 2 × 6H 2 O 0.020 MnCl2 × 4H2O 0,360MnCl 2 × 4H 2 O 0.360 Na2MoO4 × 2H2O 0,0126Na 2 MoO 4 × 2H 2 O 0.0126 Раствор №4:Solution No. 4: Na2EDTA 6,8Na 2 EDTA 6.8 Раствор №5:Solution No. 5: FeCl3 × 6H2O 5,3FeCl 3 × 6H 2 O 5.3 причем 1 л среды обогащения состоит из маточных растворов в следующем количестве, мл:moreover, 1 l of enrichment medium consists of mother liquors in the following quantity, ml: Раствор №1 0,5-2Solution No. 1 0.5-2 Раствор №2 0,5-2Solution No. 2 0.5-2 Раствор №3 0,5-2Solution No. 3 0.5-2 Раствор №4 0,5-2Solution No. 4 0.5-2 Раствор №5 0,5-2Solution No. 5 0.5-2 Морская вода ОстальноеSea water Else
RU2002117336/13A 2002-06-28 METHOD FOR ACCUMULATING A LISTERIOUS MICROBE (LISTERIA MONOCYTOGENES) RU2002117336A (en)

Publications (1)

Publication Number Publication Date
RU2002117336A true RU2002117336A (en) 2004-05-10

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