KR101675320B1 - Marine bacteria having excellent algicidal activity and method for removing red tide using the same - Google Patents

Abstract

The present invention relates to a marine bacteria having excellent algicidal activity, and to a method for removing red tide using the same. More particularly, the present invention relates to a Neptunomonas sp. marine bacteria strain having excellent algicidal activity, a bacterial formulation prepared by fixating the strain to a carrier for removing red tide, and to a method for removing red tide comprising a step of injecting at least one among the strain or the bacterial formulation to a sea area having red tide.

Description

TECHNICAL FIELD [0001] The present invention relates to a marine microorganism having excellent fungicidal activity and a method for removing red tide using the same. BACKGROUND ART < RTI ID = 0.0 >

The present invention relates to a marine microorganism having excellent fungicidal activity and a method for removing red tide using the same. More particularly, the present invention relates to a marine microorganism having excellent selective fecundity against cochlodinium polykrikoides And a red tide removing method using the same.

Red tide is a phenomenon in which microorganisms such as phytoplankton, protozoa, and bacteria, which live in the ocean in general, are massively multiplied or integrated at a time to discolor the sea water and adversely affect marine life. Red tide phenomenon causes serious damage to the fisheries industry by causing death or poisoning of fish and shellfish, and even affecting human beings, directly or indirectly causing serious problems for us.

These red tide phenomena occur widely in all coastal waters of the world, and occur regularly irrespective of seasons. Especially, red tide creatures tend to change from diatoms to monocotyledons and become denser.

The creatures that cause red tide are phytoplankton, diatom, dinoflagellate, blue-green algae, microalgae, protozoa, and more than 50 species found in Korea . Depending on the type of plankton, the color of the seawater is not limited to red but may also appear as brown, blue, or white. The representative red tide creatures in Korea are analyzed from April, 2010 to July, 2013 by the red tide bulletin of the National Fisheries Research and Development Institute and then selected by focusing on the vegetative red tide creatures with frequent occurrence, Cochlodinium polykrikoides) are the most abundant in 118 and are known to cause the most damage to fisheries due to harmful monocotyledonous birds. In addition to these, there are also other types of fish such as Chattonella marina, Heterocapasa triquetra, Scrippsiella trochoides, Skeletonema costatum, Heterosigma akashiwo, (Prorocentrum minimum) and so on.

On the other hand, known methods for removing bivalent organisms include chemical spraying, ultrasonic and ozone treatment, sea surface recovery and sedimentation, and loess spraying.

Among these methods, the most widely used chemical spraying method has been used since the past as a method of spraying copper sulfate (CuSO ₄ ), chlorine dioxide (ClO ₂ ), simazine (Simazine) and the like, The copper sulfate used is not specific to the harmful zooplankton, and it affects other marine life besides the cause of red tide. Therefore, it may cause problems in terms of toxicity and corrosion to other organisms in the water, And it is disadvantageous in that it is uneconomical because the copper sulfate is unstable under high alkaline environmental conditions accompanied with the occurrence of red tide of algae, and therefore it is required to treat a large amount.

Korean Patent No. 0407829 discloses a red tide removing method using an ultrasonic treatment method. This technique is a method of destroying cells of an organism causing red tide by ultrasonic waves. In the ozone treatment method, high pressure ozone is injected into a red tide occurrence water zone, Both methods are not yet in practical use.

The sea surface recovery and sedimentation method is widely used in Korea as a method of generating bubbles by using a pressurized floating separator composed of a centrifuge, an agglomerate, a mixing tank, and a pressurized float tank to adsorb and float the red tide organisms and recover them from the sea surface .

Korean Patent No. 0325396 discloses a red tide removing method by a loess spraying method. That is, a red tide (interfacial clay / montmorillonite) is sprayed in seawater to adsorb and settle red tide creatures, and aluminum ions in the loess It is widely used in Korea by using the property of destroying biological cells. However, caution should be exercised when the loess is sprayed in the seawater, since suspended matter increases in the fish farm and fisheries where settlement organisms live in the lower layer, which may affect the organisms such as respiratory disorders caused by fish gill closure. In addition, loess is believed to cause secondary pollution in the oceans, as the red tide creatures sink under the ocean.

Many biological methods have been attempted as another method for controlling the red tide phenomenon, and the role of bacteria in red tide phenomenon has become important.

Therefore, when the combination of new microorganisms capable of eliminating the above-mentioned problems and simultaneously removing the red tide is provided, it is expected that the present invention can be usefully applied in related fields.

Accordingly, one aspect of the present invention is to provide a marine microorganism strain having excellent lyophilicity.

Another aspect of the present invention is to provide a microorganism preparation for removing red tide comprising a marine microorganism strain having excellent lyophilicity.

Another aspect of the present invention is to provide a method for effectively removing red tide using a marine microorganism strain or microbial agent having excellent lyophilicity.

According to one aspect of the present invention, there is provided a marine microorganism strain belonging to Neptunomonas having excellent fungicidal activity.

Preferably, the strain comprises Neptunomonas sp. 2R1 (Accession No. KACC92080P).

It is preferred that the strain has a very good selective kill ability against Cochlodinium polykrikoides in red tide occurrence birds.

According to another aspect of the present invention, there is provided a microorganism preparation for vetching, wherein the marine microorganism strain of the present invention is immobilized on a carrier.

According to another aspect of the present invention, there is provided a red tide control system using the marine microorganism strain.

According to another aspect of the present invention, there is provided a red tide removing method comprising the step of putting the strain or microbial agent of the present invention into a sea area where red tide occurs.

According to the present invention, there is provided a microorganism excellent in new liveliness capable of effectively removing red tide microorganisms while causing no secondary pollution or harmful effect to other organisms, and a method of removing red tide using the microorganisms. According to the present invention, It is possible to control the red tide occurring in seawater, a farm, and the like.

Fig. 1 (a) is a graph showing the effect of the algae culture of the present invention on the growth of algae (algae) (Cochlodinium polykrikoides) is removed (microscope magnification: × 100).
FIG. 2 is a graph showing the survival rate of Cochlodinium polykrikoides (Cochlodinium polykrikoides) of marine microorganism having excellent lyophilicity according to cell number change.
FIG. 3 is a graph showing the time-dependent growth of each bacterium during the fungicidal experiment.
4 is a graph showing that Neptunomons sp. 2R1 has a selective lyophilicity against Cochlodinium polykrikoides in red tide occurrence birds.
FIG. 5 shows a diagram of a bio / eco reactor system for the red tide control, and the results thereof, applying the shale and marine microorganism strain of the present invention and a ceramic carrier.
FIG. 6 is an experimental photograph of a bio / eco reactor system for red tide control, to which the present invention is applied to a marine microorganism strain and a ceramic carrier.
FIG. 7 is a graph showing the results of an experiment (Ceramic + Bacteria) in which a bacterium was adsorbed on a carrier, in a bio / eco reactor system for the red tide control, in which a salmon and marine microorganism strain of the present invention and a ceramic carrier were applied. Cochlodinium polykrikoides < RTI ID = 0.0 > .

Hereinafter, preferred embodiments of the present invention will be described with reference to the accompanying drawings. However, the embodiments of the present invention can be modified into various other forms, and the scope of the present invention is not limited to the embodiments described below.

According to the present invention, there is provided a strain having excellent lyophilicity, a microorganism preparation in which the strain is immobilized on a carrier, and a red tide removing method using the same.

In the present invention, a strain having excellent lyophilicity means effectively killing (killing) the algae causing red tide. By such an action, the strain of the present invention can be widely used for controlling activities such as suppression or elimination of red tide.

According to the present invention, there is provided a marine microorganism strain belonging to the genus Neptunomonas, which has excellent fungicidal activity. In particular, it is preferable that the strain contains Neptunomonas sp. 2R1 (Accession No. KACC92080P). The strain is characterized by flowable anaerobic.

On the other hand, each of the strains contained in the mixed strain of the present invention was individually deposited in Korea Environmental Microorganisms Bank (KEMB). More specifically, the Neptunomonas sp. 2R1 was deposited with KEMB 3-825.

Particularly, while the marine bacterium belonging to the gamma-proteobacteria is well known as the killed bacteria, the genus Neptunomonas having the live-fighting ability according to the present invention belongs to the alpha-proteobacteria, It is almost unknown.

Furthermore, it is preferable that the marine microorganism strain having excellent survival ability is Neptunomonas sp. 2R1. The strain was deposited at the National Institute of Agricultural Science and Technology (KACC) on June 22, 2015 with P15-086, and on June 29, 2015 with the deposit number KACC92080P.

The marine microorganism strain according to the present invention has excellent survival ability against cochlodinium polykrikoides among red tide microorganisms.

According to the present invention, there is provided a microorganism preparation for red tide removal, wherein the marine microorganism strain of the present invention having the above-described superior germicidal activity is immobilized on a carrier. According to the present invention, when a strain is immobilized on a carrier and applied to a red tide generation area, stable distribution of the strain is enabled and the dilution degree in the seawater is reduced, so that the living ability can be maintained continuously.

In this case, the immobilization of the strain is carried out by adsorption. Specifically, the strain may be immobilized on the carrier by aerobically culturing the strain together with the ceramic carrier for 4 hours

Meanwhile, it is preferable that the carrier of the present invention includes at least one or more selected from the group consisting of red mud, sludge sludge, clay loam, EAF dust, expanded shale, volcanic stone and fly ash.

In particular, the carrier preferably comprises from 40 to 60% by weight of red mud, from 20 to 40% by weight of sludge sludge and from 5 to 20% by weight of pulverulent soot and further comprises from 1 to 10% by weight of EAF dust Is more preferable.

The carrier according to the present invention preferably has a water absorption rate of 15 to 25%, and when the water absorption rate is less than 15%, the bacterial retention time is shortened and the treatment time is shortened. When the water absorption rate is more than 25% There is a phenomenon that the hardness of the carrier becomes weak.

Further, the carrier preferably has a specific gravity of 0.8 to 1.6, and when the specific gravity is less than 0.8, there is a problem that the hardness is weak and it breaks well. When the specific gravity exceeds 1.6, the carrier has a good hardness but has a problem of being precipitated in water.

On the other hand, the carrier preferably has an apparent porosity of 17 to 26%. When the apparent porosity is less than 17%, there is a problem in adsorption of the strain. When the apparent porosity is more than 26%, the carrier has a low hardness.

According to another aspect of the present invention, there is provided a method for oil degradation comprising the step of introducing the mixed strain or microbial agent of the present invention as described above into a sea area where red tide occurs.

Hereinafter, the present invention will be described more specifically by way of specific examples. The following examples are provided to aid understanding of the present invention, and the scope of the present invention is not limited thereto.

Example

1. Sample collection

Four areas (Masan Bay, Jinhae Bay, Jamsom, Caguri) were selected from the offshore Tongyeong coast of the South Sea, and seawater and sediments were collected from each region as shown in Table 1 below. In the case of seawater, the conditions of dissolved oxygen and temperature are different according to the depth of seawater. Therefore, various depths of seawater were collected and mixed in consideration of different microbial distribution.

Cage Masan Bay 돝 Jinhae Bay Latitude 34 ° 59'38.19 "N 35 ° 6'12.88 "N 35 ° 11'0.44 "N 35 ° 6'55.77 "N Hardness 128 ° 40'26.38 "E 128 ° 36'14.31 "E 128 ° 34'59.31 "E 128 ° 41'7.16 "E Depth of sea water 8, 10, 15m 8, 10, 15m 8, 10, 15m 8, 10, 15m Sediment depth Sediment surface Sediment surface Sediment surface Sediment surface

The seawater samples were sampled at 20 L from each spot using a vat. The collected seawater and sediments were carried in the ice box.

2. Strain isolation

Seawater and submarine sediment samples were collected from marine areas where red tides frequently occur in the southern coast to isolate marine bacteria.

Based on the collected samples, various media were used to obtain algae microorganisms. The medium used was 10% marine agar (Difco 2216, Table 2), 10% R2A agar (MB cell, Table 3), 100% marine agar, 100% R2A agar and all the media used were microorganisms isolated from seawater For the cultivation, the composition was adjusted to 35 g / L of salinity and 35 g / L of NaCl.

The medium composition of the Marine Broth 2216 (control pH 7.6 ± 0.2) peptone 5 g Yeast extract 1 g Ferric citrate 0.1 g Sodium chloride 35 g Magnesium chloride 5.9 g Magnesium sulfate 3.24 g Calcium chloride 1.8 g Potassium chloride 0.55 g Sodium bicarbonate 0.16 g Potassium bromide 0.08 g Strontium chloride 34 mg Boric acid 22 mg Sodium silicate 4 mg Sodium fluoride 2.4 mg Ammonium nitrate 1.6 mg Disodium phosphate 8 mg D.W. 1000 ml

Composition of R2A broth (adjusted pH 7.2 ± 0.2) Proteose peptone 0.5 g Yeast extract 0.5 g The casein oxygen digest (acid digest) 0.5 g Glucose 0.5 g Soluble starch 0.5 g Dipotassium phosphate 0.3 g Magnesium sulfate 0.024 g Sodium pyruvate 0.3 g D.W. 1000 ml

The number of isolates by harvesting area and media is shown in Table 4 for aerobic conditions and in Table 5 for anaerobic conditions. The samples were collected three times in aerobic condition and anaerobic condition. The number of isolates was 3490 and 1160, respectively. Experiments were carried out using isolates, aerobic, anaerobic and anaerobic strains.

sea water Subsea sediment (Sediment) synthesis 10% MB 10% R2A 100% MB 100% R2A 10% MB 10% R2A 100% MB 100% R2A Masan Bay 63 (51) 86 (95) 88 (75) 3 (50) 113 (81) 17 (25) 15 (50) 20 (46) 405 (473) Jinhae Bay 48 (83) 28 (42) 16 (61) 16 (22) 104 (107) 24 (32) 56 (61) 16 (71) 268 (478) Cage 54 (91) 144 (125) - (18) - (41) 126 (81) 12 (18) 54 (72) - (52) 390 (598) 돝 32 (72) 24 (32) 60 (54) 60 (69) 126 (145) 12 (21) 39 (41) 39 (48) 392 (482) synthesis 197 (297) 282 (293) 164 (208) 79 (182) 469 (514) 65
(96) 124 (224) 75 (217) 3490

sea water Subsea sediment (Sediment) MB R2A 27s MB R2A 27s synthesis Masan Bay 22 (30) - (41) - (28) 45 (51) 29 (29) 28 (30) 124 (209) Jinhae Bay 39 (31) 26 (28) 10 (19) 9 (24) 10 (20) - (21) 94 (143) Cage 20 (11) 76 (11) - (32) 16 (18) 21 (51) - (18) 133 (141) 돝 52 (25) 15 (20) 7 (19) 42 (50) 38 (24) - (24) 154 (162) synthesis 133 (97) 117 (100) 17 (98) 112 (143) 98 (124) 28 (93) 1160

3. Selection and culture of red tide creatures

(1) Selection of red tide creature and medium

In order to select the most frequently occurring red tide microorganisms on the coast, the frequency of each species was investigated based on existing papers and data. At this time, we used database of the red tide related papers published in Korea, and the database of the marine fisheries science institute and the ocean science and technology institute. Among them, Cochlodinium polykrikoides (Cochlodinium polykrikoides), which is experiencing a steady increase in the frequency of occurrence and severe difficulty in control of the wide area in a short time in a short time, was used for the killing experiment. The selected red tide was sold from Seoul National University. F / 2 medium (Table 6) was used for the algae.

ingredient Stock Solution amount NaNO ₃ 75 g / L dH ₂ O 1 mL NaH ₂ PO ₄ H ₂ O 5 g / L dH ₂ O 1 mL Na ₂ SiO ₃ 9H ₂ O 30 g / L dH ₂ O 1 mL Trace metal solution (see recipe below) 1 mL Vitamin solution (see recipe below) 0.5 ml sea water 1L

Table 7 shows the composition of the trace metal solution in the f / 2 medium. Table 8 shows the composition of the vitamin solution in the f / 2 medium.

ingredient Primary Stock Solution amount FeCl ₃ 6H ₂ O - 3.15 g Na ₂ EDTA ₂ H ₂ O - 4.36 g CuSO ₄ 5H ₂ O 9.8 g / L dH ₂ O 1 mL Na ₂ MoO ₄ 2H ₂ O 6.3 g / L dH ₂ O 1 mL ZnSO ₄ 7H ₂ O 22.0 g / L dH ₂ O 1 mL CoCl ₂ 6H ₂ O 10.0 g / L dH ₂ O 1 mL MnCl ₂ 4H ₂ O 180.0 g / L dH ₂ O 1 Ml D.W 1L

ingredient Primary Stock Solution amount Thiamine HCl (vit. B ₁ ) - 200 mg Biotin (vit. H) 0.1 g / L dH ₂ O 10 mL Cyanocobalamin (vit. B ₁₂ ) 1.0 g / L dH ₂ O 1 ml D.W 1L

Because the selected red algae grow much higher density in the natural environment than in the laboratory, the culture medium and culture conditions for culturing the higher density in the laboratory have been established. The algae were cultivated at 20 ℃, 10000Lux and 3.5% (w / v) NaCl concentration. The media conditions were as follows: 1) F (2), which was prepared by high temperature and high pressure sterilization by adding F / 2 component based on seawater filtered with 0.2um filter paper (2) a medium in which the F / 2 composition is added to the seawater / filter medium, (2) the medium is not sterilized by high temperature and high pressure, (3) the vitamin solution and trace element (SL-6) Filtration of seawater filtered with 0.2um filter paper, and filtration of seawater without filter paper.

Experimental results showed that Co clonidine Polycrycoides was best grown on F / 2 medium and filtered seawater with F / 2 composition and not in high temperature and high pressure sterilized medium. The cells were cultured in a F / 2 medium sterilized at a high temperature and a high pressure for single culture.

(2) Screening

The viability of the isolates was investigated using a mixed culture of isolated marine microbial strains and selected red algae. At this time, the method used for direct counting of algae cells under a microscope was used. The strains were cultured in 0.9 ml of algae culture medium, 0.2 ml of agar and R2A agar in 24 transplate wells, collected and washed with 3.5% NaCl, and then lysed with a solution of OD 1.0 in 3.5% NaCl After 24 hours, 1 ml was transferred to the Sedgwick-Rafter chamber and the algae were fixed with 1% lugol's solution and the algae count was directly counted on the microscope.

The survival rate was calculated by referring to Equation 1 below. On the screen, strains with more than 50% viability were screened for survival and high efficiency strains.

..식(1)

(1)

The strains selected as having excellent lyophilicity against Cochlodinium polykrikoides by the above process are as shown in Table 9, and in particular, marine bacteria belonging to the γ-proteobacteria have been known as live bacteria Neptunomonas genus, which has a life-enhancing ability, belongs to α-proteobacteria, and the life-giving power of the genus Neptunomonas is almost unknown.

Strain Co-clodinium policycloides Removal rate (%) Count (number of cells / mL) Vibrio alginolyticus DS8 98.7 8.0 x 10 (control: 6.49 x 10 ³ ) Neptune Nomonas genus 2R1 98.9 7.0 x 10 (control: 6.29 x 10 ³ ) Neptunian Nosnap Toborans 2R7 98.2 1.1 × 10 ² (control: 6.20 × 10 ³ ) Swanella yupinai GM5 98.7 8.0 x 10 (control: 6.27 x 10 ³ ) Vbrionio Caledonics MS7 98.4 1.0 × 10 ² (control: 6.27 × 10 ³ )

4. High-efficiency strain By time A life force  Confirm

FIG. 1 shows the viability of each strain of the present invention by counting for 12 hours each time in order to see the change in the viability of the killed bacteria by the time of 24-hour mixed culture. At this time, changes in the survival rate were confirmed by measuring the number of cochlodinium polykrikoides.

Furthermore, 1.8 mL of the algae culture, 0.2 mL of the strain solution which had been incubated in marine agar and R2A agar, washed with 3.5% NaCl and adjusted to OD 1.0 with 3.5% NaCl was mixed and cultured, To determine the number of cochlodinium polykrikoides, the number of bacteria was counted using a hemocytometer and the number of cochlodinium polykrikoides Fig.

FIG. 1 (a) shows the chewing of Cochlodinium polykrikoides, which is algae, by the salting out of the present invention. In FIG. 1, Is a photograph on a microscope which confirms that Cochlodinium polykrikoides is removed after 12 hours of treatment with killed bacteria and C after 24 hours of treatment with killed bacteria. Fig. 1 (b) shows that cochlodinium polykrikoides, which is a red tide-induced algae, is killed by the broth culture, and A shows the treatment time 0 hours after the start of the experiment, 12 hours after treatment, and C is a microscope photograph showing that Cochlodinium polykrikoides is removed after 24 hours of treatment with the killed bacteria.

In order to confirm the reproducibility of the experiment, re-experiments were conducted based on five strains with high lyophilicity and the results are shown in FIGS. 2 and 3.

The strains used were as shown in Table 9. As can be seen from FIG. 2, Neptunomonas sp. 2R1 showed the highest survival rate as 98.9% As shown, the growth of the strain was stable at 8.29 × 10 ⁶ cells / ml.

5. Suitable for the strain of the invention Carrier  selection

The existing application method of the typhoid strains tended to be applied to the field by cultivating the killing bacteria but diluted in seawater and the effect was reduced. Therefore, we have devised a new method to eliminate the effect of dilution by continuously attaching and stabilizing a high-killing strain on the carrier and maintaining the survival ability continuously.

As a carrier for the strain having excellent lyophilicity of the present invention, five types of multi-pore ceramic carriers were prepared as shown in Table 10 below.

The strain was inoculated in 4L of optimal culture medium and incubated for 3 days at 28 ℃, which is the optimal culture temperature, for attaching the killed bacteria to the ceramic carrier. Each of the ceramic substrates is well washed to give Removal of the powder, saljo microorganism neptyu eggplant grandma (Neptunomonas sp. 2R1) the OD (600nm) focused by 0.8 strain culture ceramic aggregate of 1.26kg (about 10 ⁸ cells / ml) And the total volume was adjusted to about 3 L. The mixture was mixed at room temperature for 4 hours and adsorbed.

Red spot Sirloin Lung white EAF dust Coal fly ash Ceramic carrier-1 60 wt% 30 wt% 10 wt% - - Ceramic Carrier -2 Liapor products from Germany (expanded shale) Ceramic Carrier -3 GETPC (volcanic stone) Ceramic Carrier-4 60 wt% 25 wt% 10 wt% 5 wt% - Ceramic Carrier -5 50 wt% - - - 50 wt%

Among them, the carrier of the ceramic carrier 4 which floated on the seawater and had the best adsorption rate of the strain was used to carry out the experiment.

4, the carrier of the ceramic carrier 4 has a specific gravity of 1 or less and an absorption rate of 20% or more and a porosity (apparent porosity) of 60% or more. On the other hand, when the carrier of Production Example 4 and the 2R1 strain having the highest lyophilicity were adsorbed, it was confirmed that an average of 2.31x10 ⁷ cells / g of the bacteria was adsorbed. Therefore, when applied to algae or applied in the field, It was expected that it would be possible to maintain the survival ability by supplying strains.

6. Red tide removal experiment using the strain of the present invention

For the red tide removal experiment using the strain of the present invention, an inlet tank, a reaction tank and a flow-out tank were provided, and 2,961 cells / ml of Cochlodinium polykrikoides was introduced into the inlet tank. In the reaction tank, the salted and marine microorganism strain 2R1 of the present invention adsorbed on the ceramic carrier 2 was disposed, and as a result, 80.2% salivation was obtained in the reaction tank. Finally, finally, the effluent tank was passed through the reaction tank to contain the removed seawater and finally discharged. As a result, it was finally able to obtain a killing effect of 97.2%.

FIG. 4 is a graph showing that Neptunomons sp. 2R1 has a selective lyophilicity against Cochlodinium polykrikoides in red tide occurrence birds. FIG. 4 (a) ) Shows the results of the live performance of Neptunomons sp. 2R1 against Cochlodinium polykrikoides and Fig. 4 (b) shows results of Neptunomons sp. 2R1 against Chattonella marina Figure 4 (c) shows the results of the live performance of Neptunomons sp. 2R1 against the Prorocentrum minimum, Figure 4 (d) shows the results of the live performance of Heterocarpa triquatra (Neptunomons sp. 2R1) against Heterocapasa triquetra, Fig. 4 (e) shows the result of the firing of Neptunomons sp. 2R1 against Heterosigma akashiwo, FIG. 4 (f) (g) of the Neptunomons sp. 2R1 against the Scorpioella sp. costatum, and Figure 4 (g) shows the spawning performance of Neptunomons sp. 2R1 against the Scrippsiella trochoides .

FIG. 5 shows a diagram and a result of a bio / eco reactor system for controlling red tide, applying the shale and marine microorganism strain of the present invention and a ceramic carrier.

FIG. 6 is a photograph of a bio / eco reactor system for red tide control applying the salmon and microbial strains of the present invention and a ceramic carrier as described above.

FIG. 7 is a graph showing the results of a comparison between the results of the experiment (Ceramic + Bacteria) adsorbed on the carrier and the contents of the clodroid policycloides Cochlodinium polykrikoides) .

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments, but, on the contrary, It will be obvious to those of ordinary skill in the art.

National R & D project supporting this invention

Assignment number: NRF-2013M3A2A1067498

Department: Future Creation Science Division

Research Management Institution: Korea Research Foundation

Research Project Name: Source Technology Development Project> Public Welfare Safety Technology Development Project

Research title: Development of system for pollution control and red tide control of polluted aquaculture farms using bio / nano ceramic membrane

Contribution rate: 80

Host organization: Kyonggi University

Research period: 2013.10.01-2018.07.31

Assignment number: NRF-2015M3A9B8029697

Department: Future Creation Science Division

Research Management Institution: Korea Research Foundation

Research Project Name: Source Technology Development Project> Bio / Medical Technology Development Project> Research Material Support Project

Research Project: Microbiology and gene conservation center for environmental industry

Contribution rate: 20

Host organization: Kyonggi University

Research period: 2015.05.01-2020.04.30

Claims (7)

Neptunomonas spp., With selective fungicidal action against cochlodinium polykrikoides, among red tide outbreaks. 2R1 (Neptunomonas sp. 2R1, Accession No. KACC92080P)
A red tide generated by an algae including cochlodinium polykrikoides, which is fixed on a carrier containing 60 wt% of red mud, 25 wt% of sludge sludge, 10 wt% of scavenged soil and 5 wt% of electric furnace dust Microbial preparation for removal.
delete delete delete delete delete CLAIMS What is claimed is: 1. A method for producing a microorganism, comprising the step of introducing the microorganism preparation of claim 1 into a watershed where the blooming occurs by an algae comprising Cochlodinium polykrikoides, A method of removing red tide caused by.

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