RU2003134686A - METHOD FOR PRODUCING HYDROLYSES FROM BEES PRODUCTS FOR CULTIVATION OF SPIROCHETS - Google Patents

METHOD FOR PRODUCING HYDROLYSES FROM BEES PRODUCTS FOR CULTIVATION OF SPIROCHETS Download PDF

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Publication number
RU2003134686A
RU2003134686A RU2003134686/13A RU2003134686A RU2003134686A RU 2003134686 A RU2003134686 A RU 2003134686A RU 2003134686/13 A RU2003134686/13 A RU 2003134686/13A RU 2003134686 A RU2003134686 A RU 2003134686A RU 2003134686 A RU2003134686 A RU 2003134686A
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RU
Russia
Prior art keywords
products
cultivation
producing
spirochets
hydrolyses
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RU2003134686/13A
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Russian (ru)
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RU2279470C2 (en
Inventor
Ирина Григорьевна Кохонова (RU)
Ирина Григорьевна Кохонова
Светлана Николаевна Луцук (RU)
Светлана Николаевна Луцук
ев Валерий Анатольевич Бел (RU)
Валерий Анатольевич Беляев
Original Assignee
ФГОУ ВПО Ставропольский государственный аграрный университет (RU)
ФГОУ ВПО Ставропольский государственный аграрный университет
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Priority to RU2003134686/13A priority Critical patent/RU2279470C2/en
Publication of RU2003134686A publication Critical patent/RU2003134686A/en
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Publication of RU2279470C2 publication Critical patent/RU2279470C2/en

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  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Claims (1)

Способ получения гидролизатов из продуктов пчелиного производства для культивирования спирохет, предусматривающий обработку белоксодержащего сырья ферментативным гидролизом нативной поджелудочной железой, измельчение, отделение от растворимых примесей, нейтрализацию до рН 7,0-7,2, автоклавирование при 120 С° в течение 1 часа, отличающийся тем, что в качестве белоксодержащего сырья используют продукты пчелиного производства (обрезки, подмор, личинки трутней), при этом сырье измельчают на гомогенизаторе при 3 тыс.об./мин, затем его разводят мясо печеночным бульоном (МПБ) в соотношении 1:5, полученную суспензию обрабатывают ультразвуком мощностью 400 вт, причем экспозиция ультразвукового облучения составляет 2-3 минуты при токе 0,7 А и частоте 35 кГц, по окончании ультразвукового облучения, суспензию нейтрализуют раствором соляной кислоты или двууглекислым натрием с последующим гидролизом, центрифугирование при 3 тыс. оборотов в течение 5 мин.A method of producing hydrolysates from bee products for the cultivation of spirochetes, comprising treating protein-containing raw materials with enzymatic hydrolysis of the native pancreas, grinding, separation from soluble impurities, neutralization to pH 7.0-7.2, autoclaving at 120 ° C for 1 hour, characterized the fact that as a protein-containing raw material, bee production products are used (pruning, death, drones larvae), while the raw material is crushed on a homogenizer at 3 thousand rpm, then it is bred with hepatic broth (MPB) in a ratio of 1: 5, the resulting suspension is treated with ultrasound with a power of 400 W, and the exposure of ultrasonic irradiation is 2-3 minutes at a current of 0.7 A and a frequency of 35 kHz, at the end of ultrasonic irradiation, the suspension is neutralized with hydrochloric acid solution or sodium bicarbonate, followed by hydrolysis, centrifugation at 3 thousand revolutions for 5 minutes
RU2003134686/13A 2003-12-01 2003-12-01 Method for production of hydrolysate from apiculture products for spirochaeta cultivation RU2279470C2 (en)

Priority Applications (1)

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RU2003134686/13A RU2279470C2 (en) 2003-12-01 2003-12-01 Method for production of hydrolysate from apiculture products for spirochaeta cultivation

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RU2003134686/13A RU2279470C2 (en) 2003-12-01 2003-12-01 Method for production of hydrolysate from apiculture products for spirochaeta cultivation

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RU2003134686A true RU2003134686A (en) 2005-05-10
RU2279470C2 RU2279470C2 (en) 2006-07-10

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RU2279470C2 (en) 2006-07-10

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MM4A The patent is invalid due to non-payment of fees

Effective date: 20051202