RU2003127668A - METHOD FOR OPTIMIZING CLONAL MICROPROPORTATION OF GRAPES IN VITRO - Google Patents

METHOD FOR OPTIMIZING CLONAL MICROPROPORTATION OF GRAPES IN VITRO Download PDF

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Publication number
RU2003127668A
RU2003127668A RU2003127668/13A RU2003127668A RU2003127668A RU 2003127668 A RU2003127668 A RU 2003127668A RU 2003127668/13 A RU2003127668/13 A RU 2003127668/13A RU 2003127668 A RU2003127668 A RU 2003127668A RU 2003127668 A RU2003127668 A RU 2003127668A
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Russia
Prior art keywords
grapes
vitro
microproportation
clonal
optimizing
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RU2003127668/13A
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Russian (ru)
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RU2264706C2 (en
Inventor
Наталь Петровна Дорошенко (RU)
Наталья Петровна Дорошенко
Галина Владиславовна Соколова (RU)
Галина Владиславовна Соколова
Original Assignee
ГНУ Всероссийский НИИвиноградарства и винодели им. Я.И. Потапенко (ВНИИВиВ) (RU)
ГНУ Всероссийский НИИвиноградарства и виноделия им. Я.И. Потапенко (ВНИИВиВ)
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Priority to RU2003127668/13A priority Critical patent/RU2264706C2/en
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  • Cultivation Of Plants (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Claims (1)

Способ микроклонального размножения винограда in vitro, включающий микрочеренкование пробирочных растений с 8-10 междоузлиями на фрагменты длиной 10-12 мм с глазком и листом, высадку их на питательную среду Мурасиге и Скуга, отличающийся тем, что посадку и культивирование осуществляют на жидкой питательной среде Мурасиге и Скуга с уменьшенным количеством макроэлементов и витаминов: NH4NO3-130-140мг/л, KNO3-920-950 мг/л, Cacl22Н2О - 290-300 мг/л, MgSO47H2O - 180-191 мг/л, КН2PO4 - 65-70 мг/л, мезоинозита - 48-50 мг/л, тиамина - 0,20-0,22-мг/л, индолилуксусной кислоты 0,1-0,3 мг/л с добавлением в ее состав препарата эмистим в концентрации 10-7-10-10%.The method of microclonal propagation of grapes in vitro, including microcentering of test plants with 8-10 internodes into fragments 10-12 mm long with an eye and a leaf, planting them on a Murasige and Skoog medium, characterized in that planting and cultivation is carried out on a liquid Murasige medium and Skoog with a reduced amount of macronutrients and vitamins: NH 4 NO 3 -130-140 mg / l, KNO 3 -920-950 mg / l, Cacl 2 2Н 2 О - 290-300 mg / l, MgSO47H 2 O - 180-191 mg / l, KH 2 PO 4 - 65-70 mg / l, meso-inositol - 48-50 mg / l, thiamine - 0.20-0.22-mg / l, indolylacetic acid 0.1-0.3 mg / l with lowering the composition of the drug emistim in a concentration of 10 -7 -10 -10 %.
RU2003127668/13A 2003-09-11 2003-09-11 Method for optimization of clonal in vitro micro multiplication of grape RU2264706C2 (en)

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RU2003127668/13A RU2264706C2 (en) 2003-09-11 2003-09-11 Method for optimization of clonal in vitro micro multiplication of grape

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RU2003127668A true RU2003127668A (en) 2005-04-10
RU2264706C2 RU2264706C2 (en) 2005-11-27

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MD705Z (en) * 2013-04-19 2014-07-31 Институт Генетики И Физиологии Растений Академии Наук Молдовы Process for microclonal propagation of grapevine

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2521992C1 (en) * 2013-01-09 2014-07-10 Федеральное Государственное Бюджетное Образовательное Учреждение Высшего Профессионального Образования "Чеченский Государственный Университет" METHOD OF MICROGRAFTING GRAPES in vitro
RU2541769C1 (en) * 2013-07-30 2015-02-20 Федеральное Государственное Бюджетное Научное Учреждение "Всероссийский Научно-Исследовательский Институт Виноградарства И Виноделия Имени Я.И. Потапенко" Method of clonal microreproduction of grapes in vitro with decontamination from mycoplasmal infection
RU2538859C1 (en) * 2013-11-15 2015-01-10 Федеральное государственное бюджетное учреждение науки Институт органической и физической химии им. А.Е. Арбузова Казанского научного центра Российской академии наук Method of clonal micropropagation of grape in vitro

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MD705Z (en) * 2013-04-19 2014-07-31 Институт Генетики И Физиологии Растений Академии Наук Молдовы Process for microclonal propagation of grapevine

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RU2264706C2 (en) 2005-11-27

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Effective date: 20060912