RU2003105830A - METHOD OF DETECTION AND METHOD OF QUANTITATIVE ANALYSIS FOR HYDROGEN RADICALS - Google Patents

METHOD OF DETECTION AND METHOD OF QUANTITATIVE ANALYSIS FOR HYDROGEN RADICALS

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Publication number
RU2003105830A
RU2003105830A RU2003105830/04A RU2003105830A RU2003105830A RU 2003105830 A RU2003105830 A RU 2003105830A RU 2003105830/04 A RU2003105830/04 A RU 2003105830/04A RU 2003105830 A RU2003105830 A RU 2003105830A RU 2003105830 A RU2003105830 A RU 2003105830A
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RU
Russia
Prior art keywords
hydrogen radicals
calibration curve
absorption coefficient
dbnbs
water
Prior art date
Application number
RU2003105830/04A
Other languages
Russian (ru)
Other versions
RU2244919C2 (en
Inventor
Санетака СИРАХАТА
Казумити ОЦУБО
Original Assignee
Нихон Трим Ко., Лтд.
Санетака СИРАХАТА
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Priority claimed from JP2001160915A external-priority patent/JP3657535B2/en
Application filed by Нихон Трим Ко., Лтд., Санетака СИРАХАТА filed Critical Нихон Трим Ко., Лтд.
Publication of RU2003105830A publication Critical patent/RU2003105830A/en
Application granted granted Critical
Publication of RU2244919C2 publication Critical patent/RU2244919C2/en

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Claims (6)

1. Способ детектирования водородных радикалов, присутствующих в воде или в водном растворе, путем добавления натриевой соли 3,5-дибром-4-нитрозобензолсульфоновой кислоты (ДБНБС) к образцу для детектирования водородных радикалов по окрашиванию указанного образца.1. A method for detecting hydrogen radicals present in water or in an aqueous solution by adding the sodium salt of 3,5-dibromo-4-nitrosobenzenesulfonic acid (DBNBS) to a sample for detecting hydrogen radicals by staining said sample. 2. Способ детектирования водородных радикалов по п.1, в котором вода или водный раствор представляет собой электролитически восстановленную воду.2. The method for detecting hydrogen radicals according to claim 1, wherein the water or aqueous solution is electrolytically reduced water. 3. Способ детектирования водородных радикалов по п.1, в котором окрашивание возникает из-за пика поглощения азосоединения ДБНБС, образующегося при взаимодействии между натриевой солью 3,5-дибром-4-нитрозобензолсульфоновой кислоты (ДБНБС) и водородными радикалами.3. The method for detecting hydrogen radicals according to claim 1, wherein the staining occurs due to the absorption peak of the DBNBS azo compound formed by the interaction between the 3,5-dibromo-4-nitrosobenzenesulfonic acid (DBNBS) sodium salt and hydrogen radicals. 4. Способ детектирования водородных радикалов по п.1, детектирующий пик поглощения при длине волны от 425 до 450 нм.4. The method for detecting hydrogen radicals according to claim 1, the detecting absorption peak at a wavelength of from 425 to 450 nm. 5. Способ количественного анализа водородных радикалов, присутствующих в воде или в водном растворе, состоящий из следующих стадий (1)-(4): (1) продувки газообразного водорода через раствор 1,1-дифенил-2-пикрилгидразила (ДФПГ), имеющий поглощение вблизи 517 нм, с постоянной скоростью в присутствии платиновой черни для получения графика корреляции между уменьшением коэффициента поглощения вблизи 517 нм и временем продувки газообразного водорода (создание калибровочной кривой A); (2) взаимодействия цистеина и ДФПГ друг с другом для получения графика корреляции между уменьшением коэффициента поглощения ДФПГ вблизи 517 нм и концентрацией цистеина (создание калибровочной кривой B); (3) продувки газообразного водорода через раствор натриевой соли 3,5-дибром-4-нитрозобензолсульфоновой кислоты (ДБНБС) с постоянной скоростью в присутствии платиновой черни в течение заданного времени при таких же условиях, как в указанной стадии (1), и после этого измерения коэффициента поглощения вблизи 450 нм для получения графика корреляции между значением коэффициента поглощения и концентрацией водородных радикалов, образовавшихся в течение времени продувки газообразного водорода, вычисленной по калибровочной кривой A и калибровочной кривой B (создание калибровочной кривой C); (4) добавления натриевой соли 3,5-дибром-4-нитрозобензолсульфоновой кислоты (ДБНБС) к образцу и измерения коэффициента поглощения вблизи 450 нм для получения значения концентрации водородных радикалов по значению указанного коэффициента поглощения с помощью указанной калибровочной кривой C.5. A method for the quantitative analysis of hydrogen radicals present in water or in an aqueous solution, consisting of the following steps (1) to (4): (1) purging hydrogen gas through a solution of 1,1-diphenyl-2-picrylhydrazyl (DPPH) having absorption near 517 nm, at a constant speed in the presence of platinum black to obtain a graph of the correlation between a decrease in the absorption coefficient near 517 nm and the time of purging hydrogen gas (creating a calibration curve A); (2) interactions of cysteine and DPPH with each other to obtain a correlation graph between a decrease in the absorption coefficient of DPPH near 517 nm and the concentration of cysteine (creating a calibration curve B); (3) blowing gaseous hydrogen through a solution of the sodium salt of 3,5-dibromo-4-nitrosobenzenesulfonic acid (DBNBS) at a constant speed in the presence of platinum black for a predetermined time under the same conditions as in the indicated step (1), and then measuring the absorption coefficient near 450 nm to obtain a graph of the correlation between the absorption coefficient and the concentration of hydrogen radicals formed during the hydrogen gas purge time, calculated from calibration curve A and calibration curve B (creating a calibration curve C); (4) adding the sodium salt of 3,5-dibromo-4-nitrosobenzenesulfonic acid (DBNBS) to the sample and measuring the absorption coefficient near 450 nm to obtain the concentration of hydrogen radicals from the value of the specified absorption coefficient using the specified calibration curve C. 6. Способ количественного анализа по п.5, в котором вода или водный раствор концентрируют в 10-500 раз.6. The method of quantitative analysis according to claim 5, in which water or an aqueous solution is concentrated 10-500 times.
RU2003105830/04A 2001-05-29 2002-05-27 Method of detection and method for quantitative analysis of hydrogen radicals RU2244919C2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2001-160915 2001-05-29
JP2001160915A JP3657535B2 (en) 2001-05-29 2001-05-29 Hydrogen radical detection method and quantitative analysis method

Publications (2)

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RU2003105830A true RU2003105830A (en) 2004-06-20
RU2244919C2 RU2244919C2 (en) 2005-01-20

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US (1) US7276379B2 (en)
EP (1) EP1416271B1 (en)
JP (1) JP3657535B2 (en)
KR (1) KR100490808B1 (en)
CN (1) CN1226623C (en)
CA (1) CA2420213C (en)
DE (1) DE60235170D1 (en)
HK (1) HK1060767A1 (en)
RU (1) RU2244919C2 (en)
TW (1) TWI239394B (en)
WO (1) WO2002097427A1 (en)

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CN109884046A (en) * 2019-04-03 2019-06-14 深圳市埃克斯生物科技有限公司 A kind of rapid detection method of type of respiration acquisition free radical marker
CN112082902B (en) * 2020-07-25 2024-02-13 东北电力大学 Device for improving density of OH free radicals discharged in water and measuring method thereof
CN112946160B (en) * 2021-02-09 2022-10-25 新疆大学 Method, system and equipment for calculating contribution rate of active substance and storage medium thereof
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