RU2000126996A - METHOD FOR DETECTION AND DIFFERENTIATION OF RNA ENTEROVIRUSES - Google Patents

METHOD FOR DETECTION AND DIFFERENTIATION OF RNA ENTEROVIRUSES

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Publication number
RU2000126996A
RU2000126996A RU2000126996/13A RU2000126996A RU2000126996A RU 2000126996 A RU2000126996 A RU 2000126996A RU 2000126996/13 A RU2000126996/13 A RU 2000126996/13A RU 2000126996 A RU2000126996 A RU 2000126996A RU 2000126996 A RU2000126996 A RU 2000126996A
Authority
RU
Russia
Prior art keywords
rna
identification
differentiation
evr
poliovirus
Prior art date
Application number
RU2000126996/13A
Other languages
Russian (ru)
Other versions
RU2189396C2 (en
Inventor
Людмила Николаевна Голицина
Надежда Алексеевна Новикова
Original Assignee
Федеральное государственное учреждение науки и здравоохранения "Нижегородский научно-исследовательский институт эпидемиологии и микробиологии им. акад. И.Н.Блохиной"
Filing date
Publication date
Application filed by Федеральное государственное учреждение науки и здравоохранения "Нижегородский научно-исследовательский институт эпидемиологии и микробиологии им. акад. И.Н.Блохиной" filed Critical Федеральное государственное учреждение науки и здравоохранения "Нижегородский научно-исследовательский институт эпидемиологии и микробиологии им. акад. И.Н.Блохиной"
Priority to RU2000126996A priority Critical patent/RU2189396C2/en
Priority claimed from RU2000126996A external-priority patent/RU2189396C2/en
Application granted granted Critical
Publication of RU2189396C2 publication Critical patent/RU2189396C2/en
Publication of RU2000126996A publication Critical patent/RU2000126996A/en

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Claims (1)

Способ выявления и дифференциации РНК энтеровирусов, включающий проведение реакций обратной транскрипции ДНК методом "полугнездовой" ПЦР с последующим сравнением электрофоретических профилей кДНК, гидролизованной специфическими эндонуклеазами (рестриктазами), отличающийся тем, что используют олигонуклеотидные праймеры следующего состава: EVR 1: 5' - caccggatggccaatccaa; EVF: 5' - cggtacctttgtgcgcctgtttt; EVR 2: 5'-aattgtcaccataagcagcca; ограничивающие участок 546 нуклеотидов 5'-NTR области генома энтеровирусов, содержащий сайты для дифференцирующих рестриктаз, и набор рестриктаз применяемых для дифференциации: Sty I (для идентификации геногруппы полиовирусов), Bam HI (для идентификации РНК полиовируса 1), Ava I, Sma I (Cla I), Pvu II (для идентификации РНК штаммов полиовирусов вакцинного происхождения I, II, III типов).A method for detecting and differentiating enterovirus RNAs, including carrying out DNA reverse transcription reactions by the method of “semi-nested” PCR followed by comparison of electrophoretic cDNA profiles hydrolyzed by specific endonucleases (restrictase enzymes), characterized in that oligonucleotide primers of the following composition are used: EVR 1: 5'aaccacc; EVF: 5 '- cggtacctttgtgcgcctgtttt; EVR 2: 5'-aattgtcaccataagcagcca; bounding the 546 nucleotide region of the 5'-NTR region of the enterovirus genome containing sites for differentiating restriction enzymes and a set of restriction enzymes used for differentiation: Sty I (for identification of the poliovirus geno group), Bam HI (for identification of poliovirus 1 RNA), Ava I, Sma I ( Cla I), Pvu II (for identification of RNA strains of polioviruses of vaccine origin of types I, II, III).
RU2000126996A 2000-10-27 2000-10-27 Method of detection and differentiation of enterovirus rna RU2189396C2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
RU2000126996A RU2189396C2 (en) 2000-10-27 2000-10-27 Method of detection and differentiation of enterovirus rna

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RU2000126996A RU2189396C2 (en) 2000-10-27 2000-10-27 Method of detection and differentiation of enterovirus rna

Publications (2)

Publication Number Publication Date
RU2189396C2 RU2189396C2 (en) 2002-09-20
RU2000126996A true RU2000126996A (en) 2002-11-10

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RU2000126996A RU2189396C2 (en) 2000-10-27 2000-10-27 Method of detection and differentiation of enterovirus rna

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Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2459830C1 (en) * 2011-06-17 2012-08-27 Федеральное бюджетное учреждение науки "Государственный научный центр вирусологии и биотехнологии "Вектор" (ФБУН ГНЦ ВБ "Вектор") Set of oligodeoxyribonucleotide primers and fluorescent-marked probes for enterovirus rna identification by reverse transcription - polymerase chain reaction with hybridisation fluorescent detection
EP3878979A3 (en) * 2014-04-28 2022-01-05 Quest Diagnostics Investments Incorporated Molecular detection of enterovirus and parechovirus

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