RU1822860C - Nutrient medium for biosynthesis of penicillium rubrum pigment - Google Patents

Abstract

Usage: in biotechnology, in obtaining a nutrient medium for the biosynthesis of the pigment Penicllllum rubrum. The essence of the invention: the nutrient medium contains a source of phosphorus - KH2P04, microelements - MgS04 and ZnS04, beer wort 8 ° B - 12 ° B, rice flour and a nitrogen source - Ca (MOH) 2 4H20. 2 tab.

Description

The invention relates to biotechnology, in particular to a nutrient medium for the synthesis of food colors.

The purpose of the invention is to reduce the cost of the nutrient medium for a given strain and to expand the raw material base for the microbiological process.

This goal is achieved by the fact that in a nutrient medium containing sources of carbon, nitrogen, rice flour is used as a source of carbon, nitrogen - Ca (MO3) 2. phosphorus - KHaPO-i, trace elements - MgS04 and ZnS04. In addition, beer wort is used for dilution, with the following ratio of components, g / l: Rice flour 150-200

Ca (MOH) 2.4H201.5-2.5

KNaR040.8-1.10

MgS040.4-0.60

Zr S040.008-0.012

Beer Wort 8-12 °

Comparative analysis with the prototype allows us to conclude that there are signs that are absent in the prototype: the use of rice flour, nitrogen - Ca (MO3) g -4Н20, phosphorus - KNaROz, trace elements, ZnS04, and beer wort as a carbon source. This allows us to conclude that the claimed technical solution meets the criterion of novelty. Beer wort is widely used in microbiology (A.S. 1036048 with 12 N 1/00). Rice flour was previously used as a carbon source in the cultivation of Streptovertlcllllum hlroshimense VKPM5-894 (Z. N: 4730900/13 111245 Pol. Decision of 06/28/90) related to actinacetam. They mainly use starch-containing compounds. We did not meet the cultivation of Penlcllllum rubrum fungi in this medium in accessible sources of information. The nitrogen source Ca (NOj} 2 was previously used for the growth of microorganisms, that is, for the accumulation of biomass (Tobacco M.Ya. Effect of cultivation conditions on the biosynthesis of Oospora lactls lipase).

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Under the influence of these salts, pigment formation occurs. This property of Ca (MOH) 2 from available sources is not known to us. Trace elements MgS04, ZnS04 and KhtePO salt in a composition with carbon and nitrogen sources enhance the biosynthesis of Penlcllllum rubrum pigment. From literature data it is known that MgS04, ZnS04 and KH2P04 were used as a nutrient medium as sources of nutrients and to accelerate the accumulation of biomass (AS No. 1021679). Ca (MOH) 2.4H20 is used to isolate accumulative cultures of Galilonella Jerruglnea iron bacteria (Grand Microbiology Workshop, M., High School, 1962). This salt is found in the Liske synthetic nutrient medium). In addition, Ca (MOH) 2.4H20 is often used to cultivate soil microalgae. This allows us to conclude that the claimed technical solution meets the criterion of significant differences. The culture medium is prepared as follows. Rice flour is poured 8-12 ° B with beer wort containing inorganic salts, in the following ratio of ingredients, g / l;

Rice flour 160-200

Ca (-N03) 2-4H201.5-2.5

KH2R040.8-1.10

MgS040.4-0.60

ZnS040.008-0.012

Beer Wort

The pH of the medium was adjusted to 6.2-6.5, sterilized in an autoclave at 0.6 atm for 40 minutes.

.Example 1. 200 g of rice flour is poured 12 ° B beer wort containing, g / l:

Ca (N03) 2.4H202.5

KH2R041.10

MgS040.60

ZnS040.012

and the pH of the medium was adjusted to 6.5, sterilized in an autoclave at 0.6 atm for 40 minutes. 3% of Penlcilllum rubrum F-413 spores from the volume of medium are introduced into the sterile medium and incubated for 12 days at 30 ° C. The resulting semi-finished product is extracted with 25 ml of 96 ° alcohol and units. o.p. measured on CPK at 490 nm.

Example 2. 160 g of rice flour pour 8 ° Bl beer wort containing, g / l:

Ca (Moh) .4H201-5

KN2R040.8

MgSO40,4

ZnSCU0.008

and pH adjusted to 6.2. The remaining steps are carried out analogously to example 1

Example 3. 120 g of rice flour is poured 6 ° Bl beer wort containing, g / l:

Se (Moh) 2.4N201.0

5KN2R040.6

MgS040.2

ZnS040.006

and pH adjusted to 6.9. The remaining steps are carried out analogously to example 1. UP Example 4. 240 g of rice flour

pour 15 ° B beer wort containing, g / l:

Ca (MOH) 2.4H203.0

KN2R041.40

5MgS040.80

ZnS040.016

and the pH was adjusted to 6.8, the remaining steps are carried out analogously to example 1.

In the table. 1 shows the results of the pigment activity of Penlcilllum rubrum on known and suspected nutrient media. After culturing for 12 days on the medium of the prototype, the pigment activity was 8.0 units, and on the assumed 5, 8.8 units. The Penlcilllum rubrum F-413 strain is cultivated by a surface method. In the examples, the optimal conditions for pigment biosynthesis are given and high pigment activity is revealed. The media indicated in Examples 3, 4 showed a negative effect on pigment activity, which was accompanied by low pigment synthesis. In Example 3, pigment activity was reduced due to the minimum content of medium components. Due to which the growth and development of microorganisms is impaired. In Example 4, as a result of the high concentration of the carbon source (rice flour), the nutrient medium hardens rapidly; this impairs the development of the producer, which is accompanied by a decrease in pigment activity. Pigment activity is very weak on rice flour soaked in beer wort without mineral components. Pigment activity was measured on CPK at 480 nm.

Claims (1)

Table 2 gives the results of the cost of the proposed environment and prototype. 0 Claims Penlcilllum rubrum pigment biosynthesis medium containing a carbon source and a nitrogen source, characterized in that, in order to reduce the cost of the 5 nutrient medium, it additionally contains a phosphorus source KNgRS, trace elements MgS04 and ZnSU4 and personal wort 8 ° B-12 ° B , as a source of GLROG.DG, rice flour is used, and Ca (NaO3) 2.4H20 is used in the source of nitrogen source; in the following ratio of ingredients, g / l: Rice flour 160-200 Ca (Moh) 2.4H201.5-2.5 KN2R04 MgSO ZnSO4 Beer Wort 8 ° B-12 ° B Table 1 table 2