SU1557165A1 - Method of obtaining biologically active substances - Google Patents

Abstract

The invention relates to the microbiological industry and can be used to obtain biologically active substances, such as vitamins, biomass, and aromatics. The aim of the invention is to increase the yield of the target products. The product of a biologically active substance is cultivated under conditions of aeration on a nutrient medium containing sources of hydrocarbon, nitrogen, mineral salts and a liquid fraction separated from waste after hydrodistillation of essential oil froz at the rate of 0.05-3.0 g of dry substances per 1 liter of medium. The introduction of this waste into the composition of the medium allows an increase in the biomass yield by 16.0-35.6%, of an essential oil by 20.1-99.6%, of a vitamin by 14.8-53.8% compared with known methods. 2 tab.

Description

The invention relates to the microbiological industry and can be used to obtain biologically active substances, such as vitamins, biomass, and aromatics.

The purpose of the invention is to increase the g-si course of the target products of microbiological synthesis.

The method consists in cultivating the producer of the biologically active substance under conditions of aeration on a nutrient medium containing sources of carbon, nitrogen, mineral salts and a liquid fraction separated from the waste after hydrodistillation of essential rose oil, containing 4.8-5.4 % water soluble metabolites

rose flowers, while the waste is introduced into the environment at the rate of 0.05-3.0 g of dry matter per 1 l of medium.

These experiments are presented in table 1 and 2.

Example 1 For the cultivation of a producer, 1% glucose, 0.3% peptone, 0.05% yeast extract, 0.15% sodium hydroxide, 0.05% K.LPO and 14 mg% inositol and 2 ml are mixed in water. / l (0.1 g DM) of the liquid phase of waste rose processing. The pH is adjusted to 6.5 with KOH solution. The medium prepared in this way is sterilized, cooled and seeded with the drozek-like fungus Eremo- thecium ashbyi. Cultivated with

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with aeration for 48 hours. 3.30 g / l of dry biomass is obtained,

54.8mg / l riboflavin and 145.6 mg / l aromatherapy substances.

When growing this culture on the medium of the indicated composition under the conditions described, but without adding the liquid phase of rose waste, 2.36 g / l of dry biomass was obtained,

35.9mg / l riboflavin and 124.4 mg / l aromatherapy substances

| Example 2. Prepare the medium in Example 1. Sow the medium with the mycelium Ashbya gossipii fungus and culture it under the conditions described. 1.38 g / l of dry biomass, 56.0 mg / l of riboflavin are obtained.

On the medium prepared without water-soluble substances of rose flowers, 1.19 g / l of dry biomass and 48.8 mg / l of riboflavin were obtained.

Example 3. Prepare the medium by dissolving the malt wort with water up to 7B and add 1 ml / l (0.05 g C of the liquid phase of the rose rose plant. The initial pH 6.6 of the medium is adjusted with KOH solution. The prepared medium is sterilized, cooled, given and sow E. ashbyi. Cultivate at 27 ° C with aeration for 49 h. Obtain 2.21 g / l of dry biomass, 45.0 mg / l of riboflavin and 150.7 mg / l of air-forming substances In the control experiment (without adding liquid phase of waste from rose processing) 1.63 g / l of dry biomass, 20.2 mg / l of riboflavin, 124.0 mg / l of aromatheating agents were obtained

Example 4. Mixed in water 2%, soy flour, 1% sucrose and 60 ml / l (3.0 g DM) of the liquid phase of the rose flower processing waste, bring the volume of the medium to 1 l with water. pH 6.8 is adjusted with a solution of HCl. The medium thus obtained is sterilized, cooled to 28 ° C, and E. ashbyi is seeded. Cultivated by aeration for 49 hours. I get a deep culture with a content of 28.6 mg / l riboflavin and 212.2 mg / l aromatherapy substances.

In the control, 25.2 mg / L of riboflavin and 138.0 mg / L of aromatherapeutic substances were obtained (medium without the addition of water-soluble substances of flowers

roses).

Example 5. Prepare the medium in Example 4, but additionally add 2 ml / l of the liquid phase of the waste.

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five

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ki roses. The medium is sown with E. ashbyi and cultured under the conditions described for 68 hours. A depth culture is obtained with a content of 430.0 mg / l | Riboflavin and 544.2 mg / l of aromatic product.

In the control experiment (without adding water-soluble substances of the rose), 424.0 mg / l of riboflavin and 473.1 mg / l of aromatic product were obtained.

Example 6. Prepare the medium of Example 5. A. gossipii medium is seeded and cultured under the conditions described. 39.8 mg / l riboflavin is obtained (in the control experiment 32.4 mg / l).

Example 7. In water, 1% glucose, 0.4% peptone, 0.2% succinic acid, 0.1% NH4C1, 0.05%, 0.01% MgSO4 are mixed, and 2 ml / l of the liquid phase is additionally added. waste recycling. The medium is sown with E. ashbyi and cultured at 29 ° C with aeration for 50 hours. A deep culture is obtained containing 3.02 g / l of dry birmass, 31.6 mg / l of riboflavin and 135.0 mg / l of aromatherapeutic substances.

In the control experiment (without adding water-soluble substances of rose flowers) the amount of biomass is 2.56 g / l, riboflavin - 26.8 mg / l, aromatherapeutic substances - 112.5 mg / l.

Example 8. The culture of the literary bacterium E. coli is grown under aeration conditions at 37ffC on a nutrient medium of the following composition, g / l: peptone 100.0; sodium chloride 50, O; sucrose 5.0; water-soluble substances of rose flowers 0.05; water plumbing rest. After 24 hours of growth, the biomass concentration is 1.43 g W / l, in the control - 1.13 g W / l.

Example 9. The culture of the gram-positive bacteria Staphylococcus epidermidis is grown under aeration conditions at 37 ° C on a nutrient medium of the following composition, g / l: peptone 100.0; sodium chloride 50.0; sucrose 5.0; water-soluble substances of rose flowers 0.5; water plumbing rest. The concentration of biomass after 24 hours of growth is 2.92 g W / l, in the control - 2.37 g W / l.

The method allows to increase the biomass yield by 16.0-35.6%, of the essential oil - by 20.1-99.6%, of vitamin by 14.8-53.8% compared with the known methods, while reducing the cost price of the target product 1.2-2.0 times.

Claims (1)

Invention Formula The method of obtaining biologically active substances, involving the cultivation of the producer on a nutrient medium containing sources carbon, nitrogen and mineral salts} with aeration up to maximum accumulation of the target product, characterized in that, in order to increase the yield of the target product, the liquid phase, separated from the waste after hydrodistillation of the essential rose, is additionally introduced into the nutrient medium, 05 - 3.0 g of dry matter per 1 liter of medium. Table I The effect of the amount of dry and environmental othola in the medium on the biosynthesis of biologically active substances Table2 Efficiency of application of waste processing of raw essential oil Microorganism name i Soyeva Wednesday + 3.0 g / l VVTSR Malt wort + + 0.05 g / l VVTSR Soyeva Wednesday + 0.1 g / l VVCR Peptone-yeast medium + 0.1 g / l VVCR. Neptone-sugar environment + 0.05 g / l of VVCRR der-Peptone-sugar Wednesday +0.5 g / l VVCR as part of the environment 153.8 121.5 122.8 114.8