RU1802374C - Method for forming smaller ventriculus of animals - Google Patents

Description

 The invention relates to experimental biology, in particular to methods for the operative preparation of animals for study} and gastric function.

 The aim of the invention is to bring the experimental conditions closer to the natural physiological conditions by preserving the CBJ of the small ventricle with the main stomach through the intramural nerve plexuses.

 In FIG. 1 shows a general view of the stomach in the first stage of formation of the small stomach; in FIG. Figures 2 and 3 show the options for removing one of the subsequent steps of the walkie-talkie; in FIG. 4 - a general view of the formed small ventricle upon completion

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walkie-talkies: in FIG. 5 - the location of the small ventricle on the abdominal wall of the dog; on phi (. 6 - a method of temporarily isolating the small ventricle using clamps: in Fig. 7, a clamp for clamping the neck of the small ventricle.

 The invention is carried out as follows

In an animal, such as a dog, surgery is performed under general anesthesia. Stomach 1 is removed through the middle section of the abdominal wall (Fig. 1.4), its walls are stretched and two pulp are applied parallel to the general curvature. Then parallel to the pulp and at the same distance from them, an incision is made in the anterior and posterior walls of the stomach, not bringing the incision to the edges on both sides by about 3 cm. After that, on the free edges of the dissected walls of the big stomach 1 and separately the small ventricle 2 (Fig. 1 ) impose two-story seams. The small ventricle 2 maintains communication with the large stomach 1 through the tubular isthmuses 3 and 4 (Figs. 1-4), which

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adjoin, respectively, to the fundus and pyloric sections of the stomach. A fistula tube 5 is inserted into the small ventricle 2 (Fig. 2-4) and fixed with purse string sutures. Part of the blood vessels approaching the greater curvature of the stomach through the omentum is ligated and intersected so that the middle part of the small ventricle 2 is freed from the omentum, which may interfere with subsequent stages of the operation. Then, in both corners of the operative wound, metal arches 6 and 7 (Fig. 2) are fixed with ligatures to the edges of the abdominal muscles, with which the surgical wound in these areas is expanded. Then, the middle sections of the opposite muscle edges of the surgical wound are stitched together for about 5 cm so that the resulting muscle jumper 8 (Fig. 2-4) passes through the slotted window 9 (Fig. 1) formed in the body of the stomach during the formation of the small ventricle 2. Due to the fact that the rigid metal arches 6 and 7 (Fig. 2) in the places of their overlap do not allow the edges of the incision to come closer to each other, the pinching of the isthmuses 3 and 4 is prevented and their patency is preserved . The formation of widened holes in the abdominal wall through which the neck of the small ventricle passes can also be achieved by tightening sutures 10 (Fig. 3) on the free edges of the abdominal muscles adjacent to the neck.

At the next stage of the operation, the skin on the right and left sides of the surgical wound is dissected, resulting in the formation of pockets with a depth of 7-8 cm. Then, at a distance of about 6 cm from the skin edges at the level of the bridge 8 (Fig. 2, 3) and parallel to the median the lines make two through skin incisions 11 (Figs. 2, 3), approximately 5 cm long. Next, the lateral and separately medial edges of these sections along their entire length are sutured to each other over the jumper 8. Then the free edges of the skin are sutured together along the middle cut, thereby completing the formations the skin clutch 12 (Fig. 4), into which the small ventricle 2 is enclosed. In this case, a sap-like skin window 13 is formed between the abdominal wall and the small ventricle 2 (Fig. 4, 6).

5-7 days after surgery, skin sutures are removed. Experiments are started in 3-4 weeks. During the experiment, clamps 14 (Fig. 6, 7) with parallel jaws are placed on the isthmuses of the small ventricle 2. In this case, the walls of the isthmuses come together (do not squeeze), as a result of which the cavities

the large and small ventricles are disconnected. Then the fistula tube 5 is opened. If food remains are left in the isolated small ventricle, then

eat washed with warm saline. Gastric juice, which is separated in response to a particular effect, is collected in a vessel tied to the body of the animal (not shown in the figure). The peculiarity of the clamps 14 used is that they do not squeeze the tissue, and only close the walls of the isthmuses 3 and 4 rather densely. At the same time, neither the blood supply nor the neural connections of the small ventricle are broken, as evidenced, in particular, juice removal curves in response to nutritional, hormonal and other influences.

Beyond experience, when the cavity is large and

Since the small ventricles communicate freely through the tubular isthmuses 3 and 4, all parts of the stomach, as before the operation, take part in digestion. This eliminates chronic loss of gastric juice. The intactness of the anatomical relationship between the small ventricle and the cardiac and pyloric regions of the rest of the stomach determines the preservation of the normal nerve connections of the isolated

0 ventricle, which is a very important circumstance, since in addition to the direct innervation of the small ventricle for its adequate functioning, it also matters how widely the intramural neural plexuses of the small ventricle are connected with the intramural plexuses of the big stomach. The proposed method, due to the presence of two isthmuses formed from all layers of the wall of the stomach, in comparison with the known methods provides the greatest safety of the connections of the small and large stomachs through the intramural nerve plexuses. All these

5, the features of the new method make it more physiological and, unlike the prototype method, provide more complete information about the functioning of the digestive system.

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One of. significant disadvantages of the known methods is that animals out of experience constantly lose gastric juice, which through the fistula enters

5 skin, causing irritation and ulceration. This makes it very difficult to care for such animals. In animals operated on by the proposed method, the fistula tube is closed by a stopper outside the experiment and the loss of gastric juice is excluded.

Thanks to this, the care of such animals is greatly facilitated.

Claims (1)

SUMMARY OF THE INVENTION A method of forming a small ventricle in animals, comprising mechanically isolating part of the stomach from the cavity of the rest of the stomach, applying a fistula to the small ventricle, characterized in that, in order to bring the experimental conditions closer to natural physiological conditions due to the preservation of the connections of the small ventricle with the rest of the stomach through the intramural nerve plexuses, the small ventricle is formed in the region of large curvature, the tubular isthmuses at the border to the pyloric and cardinal parts of the remaining stomach are preserved, the small ventricle is removed from the abdominal cavity and enclose it in a skin clutch. W , x /4 ШШ