RU1779331C - Apparatus for liquid desquamation of tissue cells of tubular organs - Google Patents

Abstract

Usage: medicine, in devices for liquid desquamation of tissue cells of any cavity formations. The device comprises a housing in the form of a cylinder 2 with end caps, in which fittings 5 and 6 are installed to secure the ends of the investigated hollow organ 1 and a pump included in a closed circuit. One of the caps has a through channel for communication of the cylinder cavity with the atmosphere, and in the second plug - a channel with a fitting for supplying a working medium to the cylinder cavity. 2 ill.

Description

2

The invention relates to the field of medicine, namely to devices for liquid desquamation of animal tissue cells of any cavity formations.

A known method for determining the adhesion of animal cells to tissues, defined by the term Liquid Disintegration, the essence of which is to determine the rate of separation of cells by a stream of isotonic liquid with the determination of the breaking force of the stream. To implement the method proposed above, the authors used two types of disintegrants. They are recognized by us as analogues.

The closest analogue we have chosen is the Method for determining the limitation of the onset of death of A.S. USSR No. 602547.1977, MKI 2 C 12 4 1/00. V.I. Nevodnik, V.I. Arkhipenko, V.D. Vasyuchenko (p.ototp and p). The device used in this method contains a structure that provides liquid desquamation of the cells of the tissues of the tubular organs, which makes it possible to use it as a prototype. When using this device, cells are removed from pieces of tissue from the surface of the piece with a moving stream of liquid. At the same time, a piece of tissue has a rectangular shape of a strictly defined size, respectively, of the area of the disintegrator chamber. A piece of tissue is fixed in the chamber so that a narrow gap is located between the tissue surface and the chamber wall. The fluid passing over the surface of the tissue in this gap tears off the cells. A stream of fluid is directed to the surface of the tissue by the plunger of the syringe; therefore, cell detachment occurs cyclically when the plunger moves the syringe. Up is the phase of expelling the liquid, down is the phase of filling the syringe with liquid. The movement data of the syringe (or rather, its piston) is produced by an electromechanical drive consisting of an electric motor, a fixture for fixing the chamber and a syringe, the piston of which is connected to the gearbox of the motor. In this case, the piston is given a cyclic movement: up and down.

The disadvantages of the known method based on the work of the disintegrator include: 1) the possibility of using it to study the adhesion of animal tissue cells only flat pieces, which is due to the design features of the disintegrator; 2) the impossibility of using the known disintegrator to study the adhesion of cells of hollow cavity formations, since the design of the chamber excludes the placement and fixation of cavity formations in it; 3)

the possibility of using the known disintegrator only for a certain standard size of the studied pieces of tissue, limited by the size

the chamber of the disintegrator; 4) the impossibility of conducting the study under conditions as close as possible to physiological, which is explained by the design features of not only the tissue of the chamber itself, but also by the design of the electromechanical drive; 5) the cyclic movement of the jet of desquamating fluid (up and down) over the surface of a piece of the tissue under study . which does not create a fluid movement in

the direction that corresponds to its movement in a living organism; 6) the inability to provide the desquamating fluid of those parameters that are presented during the experiment to

liquids in biological research (parameters: speed, pressure, flow, temperature, etc.).

The aim of the invention is to maintain stable conditions for the desquamation of cells throughout the internal volume of the test organ.

The goal is achieved in that the device contains two fittings on which the cavity formation is fixed

(for example, a vessel, intestine, duct), placed in a system of hermetically closed circuits (circuits), in which the movement of desquamating fluid in a direction adequate to the movement of

other substrates in a living organism. The essence of the proposed device is illustrated by drawings and graphs, photographs. Figure 1 schematically depicts. wife device, namely: hermetically

a closed loop of the desquamating liquid with the disintegrator / 2 / turned on, in which the studied object / 3 / was fixed. As the feed pump / 1 / can be used any pump volume-pulsating type, providing pumping desquamating fluid through the pipeline / 4 / and cavity formation / 3 /. In FIG. 2 shows a sectional view of a disintegrator with a fixed cavity formation / 1 / included in a hermetically closed system of desquamating fluid. In FIG. 2 shows that the disintegrator consists of a rigid hollow transparent cylinder / 2 /, the ends

which are glued into the grooves of the metal tips / 3,4 / (epoxy adhesive can be used as a bonding material). The investigated cavity formation / 1 / is fixed on removable

nipples / 5, 6 / (for secure fit

impose ligatures). Depending on the object of study, part of the removable fittings on which the object under investigation / 5a, 66 / is fixed can be made of different sizes, i.e., of different diameters, without changing its other components. The removable fittings / 4, 5 / and the corner fittings / 7, 21 / are equipped with fixed, freely rotating clamping nuts / 8 / and O-rings / 9 / for connecting to the corner fittings / 7.21 / and the removable fittings / 4, 5 / that provides a hermetic connection to the pipeline / 10 /. In addition, a removable nozzle / 5 / along the circumference of the circumference from the outside is provided with slots (not shown in the drawing) for convenience when tightening the specified nozzle / 5 / in the end / 3 /. For a better understanding of the essence of the proposed invention, we pay attention to the fact that the connection between the tips / 3,4 / and the removable fittings / 5, 6 / is provided with sealing ring gaskets / 11, 12 /, and the removable fitting / 6 / in the tip / 4 / - clamping nut / 13 /. Through the hole / 18 /, air is released from the system, after which the hole / 18 / is closed by a plug / 19 /, which is threaded. The channel / 15 / is connected south with the hole / 20 / by means of a flexible pipe, thereby achieving the same pressure in the circulating fluid system in the desquamating fluid circuit and in the annular cavity. /14/. For this, channels / 16, 17 / are made in the tips / 3, 4 /. The annular cavity (14) is filled through the hole (15) with a liquid identical to the desquamating one to create pressure on the cavity formation. After filling the annular cavity / 14 / with liquid and venting the air through the hole / 18 /, the channel / 17 / is closed with a threaded plug / 19 /. The channel / 16 / is connected by a flexible conduit to the hole / 20 / in the corner fitting / 21 /, which creates pressure on the studied cavity formation (identical in the system of desquamating fluid), balancing the pressure in its lumen, adequate pressure on it with side of soft tissues, organs in physiological conditions of a living organism. The hole / 20 / in the corner fitting / 21 / simultaneously serves to release the desquamating liquid after examination (system, liquid discharge on the drains is not shown). An opening / 22 / in the corner fitting / 7 / is provided for discharging air from the system when it is filled with a desquamating liquid. The outlet / 22 / is equipped with a threaded plug. The removable nozzle / 6 / has an internal thread / 23 / for connection with the auxiliary rod when conducting the cavity formation fixed on the nozzles through the nozzles / 3 /. After pumping the liquid (the exposure to pumping the desquamating liquid is selected depending on the object and purpose of the study), through the cavity formation, it is discharged through the hole / 20 / in the corner fitting / 21 / for further research. At the same time, open the plug / 22 /, upon opening which conditions are created for the release of the desquamating fluid from the system. Depending on the purpose, objectives and object of study, the disintegrator can be made of any size. The movement of the flow of desquamating fluid in a closed circuit in a direction adequate to its movement and the conditions of a living organism, its pressure, flow rate, speed and temperature are ensured by the use of the electromechanical part of the apparatus manufactured by the domestic industry, artificial heart-lung ISL-2. in accordance with the conditions of its circulation in the body (for example, for the femoral artery, the blood pressure is 120 mmHg with systole and 80 mmHg with diastole; speed 660 mm / s with systole and 600 mm / s with diastole; flow rate 5 l per minute; temperature 37 ° C). The device YSL-2 is equipped with a volume-pulsating-type roller pump, which provides a predetermined flow rate of desquamating fluid, as well as a system for monitoring its parameters, which allows monitoring its pressure (pressure gauge), speed (tachometer) and flow rate, which is controlled in depending on engine speed. The shaft of the electric motor is connected by means of a gearbox to the crown of the pump, equipped with rollers, the degree of pressure of which on the polyurethane pipes is also regulated. The polyurethane pipelines are heated from the outside by friction of the pump rollers, which ensures that the desquamation fluid inside the pipelines is heated to a temperature of 37 ° C. The mounted disintegrator is included in a hermetically closed loop system. Desquamating fluid by screwing union nuts / 8 / with elbow fittings / 7. 2 1 /. After that, the system is filled with desquamating fluid (e.g., saline) through openings / 20, 22 / made in corner fittings. After the study (exposure in

Depending on the purpose and object of the study, the desquamating fluid containing the plucked cells is drained, stained, the volume is measured, and the counting chamber is filled with it to count the desquamated cells per unit time. The system in each study is washed and prepared for the next experiment,

In addition, the design principle of a disintegrator included in a hermetically closed circuit with a flowing liquid can be used not only in the study of biological objects, but also in studying the effects of various chemical and other liquids on the surface (both external and internal) of hollow objects of different forms and quantities.

Using the proposed device - Disintegrator for liquid desquamation of tissue cells of cavity formations in comparison with existing disintegrators has the following advantages:

1) the possibility of its use in studying the adhesion of cells of integral cavity formations (for example, a vessel, cell, duct, etc.);

2) the ability to conduct research under conditions as close to physiological as possible;

3) the ability to provide a desquamating fluid of those parameters that are as close as possible to the physiological conditions for the existence of the investigated organ.

All of the above features can improve the accuracy of the study.

adhesion of cellular elements, and therefore increase the accuracy of determining the age of death. The device is easy to use, accessible for operation. For its manufacture no additional

Claims (1)

instruments and devices, other than those produced by domestic industry. SUMMARY OF THE INVENTION A device for liquid desquamation of tissue cells of tubular organs, comprising a housing with means for securing the ends of the tubular organ and a pump included in a closed circuit, characterized in that, in order to maintain stable conditions for desquamation of cells throughout the internal volume of the investigated organ by creating back pressure on the outside of its walls, the housing is made in the form of a cylinder with end caps, in which there are placed along the cylinder axis of the hole for installing means for fastening the ends of the tubular organ in the form of fittings protruding into the cylinder cavity, while in one of the plugs there is a through channel for communication cavity of the cylinder with the atmosphere, having a socket for installing the plug, and in the other plug is a channel co. a nozzle for supplying a working medium to the cylinder cavity.